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公开(公告)号：US11203018B2
公开(公告)日：2021-12-21
申请号：US16160816
申请日：2018-10-15
Applicant: Berkeley Lights, Inc.
Inventor： Troy A. Lionberger , Matthew E. Fowler , Phillip J. M. Elms , Kevin D. Loutherback , Randall D. Lowe, Jr. , Jian Gong , J. Tanner Nevill , Gang F. Wang , Gregory G. Lavieu , John A. Tenney , Aathavan Karunakaran , Anupam Singhal , I-Jong Lin
Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.
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2.发明申请公开(公告)号：US20200171501A1
公开(公告)日：2020-06-04
申请号：US16661310
申请日：2019-10-23
Applicant: Berkeley Lights, Inc.
Inventor： Jason M. McEwen , Magali Soumillon , Shao Ning Pei , Randall D. Lowe, Jr. , Samira A. Nedungadi , Volker L.S. Kurz , Jian Gong , Yara X. Mejia Gonzalez , Mckenzi S. Toh , Brian A. Rabkin , Jason C. Briggs , Darcy K. Kelly-Greene , James M. Porter, Jr.
Abstract: Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided for processing biological cells, e.g., for use in nucleic acid library preparation and/or synthesis (including amplification). The devices include a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode. Methods of nucleic acid library preparation and/or synthesis can involve providing reagents to cells or nucleic acids by merging appropriate droplets on a droplet actuation surface within a water-immiscible organic liquid and can be performed in the presence of appropriate surfactants. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are systems for temperature control of the microfluidic device during nucleic acid library preparation and/or synthesis which can reduce temperature overshooting during heating and cooling steps.
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公开(公告)号：US10973227B2
公开(公告)日：2021-04-13
申请号：US15136777
申请日：2016-04-22
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, Jr.
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
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公开(公告)号：US11007520B2
公开(公告)日：2021-05-18
申请号：US16196649
申请日：2018-11-20
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, Jr. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC: B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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公开(公告)号：US10723988B2
公开(公告)日：2020-07-28
申请号：US15135707
申请日：2016-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, Jr. , Kristin Beaumont , Aathavan Karunakaran , Natalie Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
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Patent Agency Ranking
6.发明授权公开(公告)号：US10712344B2
公开(公告)日：2020-07-14
申请号：US15406289
申请日：2017-01-13
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , George L. Fox , Peggy A. Radel , Mark P. White , Xiaohua Wang , Minha Park , Guido K. Stadler , Randall D. Lowe, Jr. , Xiao Guan Radstrom , Jason M. McEwen , Gang F. Wang
IPC: B01L3/00 , G01N33/574 , C07K16/00 , G01N33/569 , C07K16/28 , G01N33/50 , C07K16/30 , C12Q1/6886 , G01N33/543
Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.
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公开(公告)号：US10101250B2
公开(公告)日：2018-10-16
申请号：US15136763
申请日：2016-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Randall D. Lowe, Jr.
IPC: G01N1/34 , B01L3/00 , B03C5/00 , B03C5/02 , C12Q1/6806 , G01N27/447
Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.
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公开(公告)号：US12102083B2
公开(公告)日：2024-10-01
申请号：US17228058
申请日：2021-04-12
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, Jr.
CPC classification number: A01N1/0284 , A01N1/0221 , A01N1/0263 , B01L3/502715 , B01L3/502761 , C12M47/04 , B01L2200/0668 , B01L2300/021 , B01L2300/024 , B01L2300/0864 , B01L2300/16 , B01L2300/163 , B01L2300/18 , B01L2300/1894
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
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公开(公告)号：US11789016B2
公开(公告)日：2023-10-17
申请号：US16849811
申请日：2020-04-15
Applicant: Berkeley Lights, Inc.
Inventor： Troy A. Lionberger , Phillip J. M. Elms , Anupam Singhal , Randall D. Lowe, Jr. , Volker L. S. Kurz , Paul M. Lebel
IPC: B01L3/00 , G01N33/543 , G01N33/68
CPC classification number: G01N33/54366 , B01L3/502761 , G01N33/6854 , B01L2200/0668 , B01L2300/0654 , B01L2300/0864 , B01L2300/165 , B01L2300/1822 , B01L2400/0424 , B01L2400/0457 , B01L2400/0472 , B01L2400/086
Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.
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公开(公告)号：US11365381B2
公开(公告)日：2022-06-21
申请号：US16920365
申请日：2020-07-02
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, Jr. , Kristin G. Beaumont , Aathavan Karunakaran , Natalie C. Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
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