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1.发明授权Cytochrome p450 monooxygenases and their use for oxidizing organic compounds 有权细胞色素p450单加氧酶及其用于氧化有机化合物的用途公开(公告)号：US07960155B1
公开(公告)日：2011-06-14
申请号：US10031146
申请日：2000-07-27
申请人： Bernhard Hauer , Juergen Pleiss , Ulrich Schwaneberg , Jutta Schmitt , Markus Fischer , Rolf Schmid , Qing-shan Li , Sabine Lutz-Wahl , Daniel Appel
发明人： Bernhard Hauer , Juergen Pleiss , Ulrich Schwaneberg , Jutta Schmitt , Markus Fischer , Rolf Schmid , Qing-shan Li , Sabine Lutz-Wahl , Daniel Appel
IPC分类号： C12N9/02 , C12N1/20 , C12N15/00 , C12P7/00 , C12P21/04 , C12Q1/26 , C12Q1/00 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/0071 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/6409 , C12P17/10 , C12P17/165 , C12Q1/26 , G01N2333/90245
摘要： The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.
摘要翻译： 本发明涉及包含修饰的底物特异性的新型细胞色素P450单加氧酶，其编码的核苷酸序列，含有这些序列的表达构建体和载体以及与其转化的微生物。 本发明还涉及用于微生物氧化不同有机底物的方法，例如生产靛蓝和靛玉红的方法。
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公开(公告)号：US20100267083A1
公开(公告)日：2010-10-21
申请号：US12422666
申请日：2009-04-13
申请人： Bernhard Hauer , Juergen Pleiss , Ulrich Schwaneberg , Jutta Schmitt , Markus Fischer , Rolf Schmid , Qing-shan Li
发明人： Bernhard Hauer , Juergen Pleiss , Ulrich Schwaneberg , Jutta Schmitt , Markus Fischer , Rolf Schmid , Qing-shan Li
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0077 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/42 , C12P17/10 , C12P17/165 , C12Q1/26 , G01N2333/90245
摘要： The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.
摘要翻译： 本发明涉及具有改变的底物轮廓的修饰的细胞色素P450单加氧酶，其编码的核酸序列，表达构建体和载体，包含这些载体的重组微生物，以及用于微生物生产末端或中性羟基化脂族羧酸的方法 酸。
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公开(公告)号：US07981652B2
公开(公告)日：2011-07-19
申请号：US12422666
申请日：2009-04-13
申请人： Bernhard Hauer , Juergen Pleiss , Ulrich Schwaneberg , Jutta Schmitt , Markus Fischer , Rolf Schmid , Qing-shan Li
发明人： Bernhard Hauer , Juergen Pleiss , Ulrich Schwaneberg , Jutta Schmitt , Markus Fischer , Rolf Schmid , Qing-shan Li
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0077 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/42 , C12P17/10 , C12P17/165 , C12Q1/26 , G01N2333/90245
摘要： The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.
摘要翻译： 本发明涉及具有改变的底物轮廓的修饰的细胞色素P450单加氧酶，其编码的核酸序列，表达构建体和载体，包含这些载体的重组微生物，以及用于微生物生产末端或中性羟基化脂族羧酸的方法 酸。
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公开(公告)号：US07531335B1
公开(公告)日：2009-05-12
申请号：US10031695
申请日：2000-07-27
申请人： Bernhard Hauer , Juergen Pleiss , Ulrich Schwaneberg , Jutta Schmitt , Markus Fischer , Rolf Schmid , Qing-shan Li
发明人： Bernhard Hauer , Juergen Pleiss , Ulrich Schwaneberg , Jutta Schmitt , Markus Fischer , Rolf Schmid , Qing-shan Li
IPC分类号： C12N9/02 , C12N15/00 , C12N1/20 , C12P7/00 , C12P21/04 , C12Q1/26 , C07H21/04 , C12Q1/00 , C12Q1/68
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0077 , C12P7/02 , C12P7/04 , C12P7/22 , C12P7/42 , C12P17/10 , C12P17/165 , C12Q1/26 , G01N2333/90245
摘要： The present invention relates to modified cytochrome P450 monooxygenases with an altered substrate profile, to nucleic acid sequences coding therefor, to expression constructs and vectors, to recombinant microorganisms which comprise these vectors, and to processes for the microbiological production of terminally or subterminally hydroxylated aliphatic carboxylic acids.
摘要翻译： 本发明涉及具有改变的底物轮廓的修饰的细胞色素P450单加氧酶，其编码的核酸序列，表达构建体和载体，包含这些载体的重组微生物，以及用于微生物生产末端或中性羟基化脂族羧酸的方法 酸。
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公开(公告)号：US20100151502A1
公开(公告)日：2010-06-17
申请号：US12295889
申请日：2007-04-05
申请人： Bernhard Hauer , Dietmar Häring , Cecilia Kvarnstroem-Branneby , Rolf Schmid , Monika Müller , Danni Liu
发明人： Bernhard Hauer , Dietmar Häring , Cecilia Kvarnstroem-Branneby , Rolf Schmid , Monika Müller , Danni Liu
IPC分类号： G01N33/554 , C12N9/20 , C07H21/00 , C12N15/74 , C12N1/21
CPC分类号： C12N9/20 , C12Q1/44 , C12Q1/61 , G01N2333/918
摘要： The present invention relates to nucleic acids that code for triacylglycerol lipases, vectors comprising said nucleic acids, host cells comprising said nucleic acids or vectors, methods for the expression of triacylglycerol lipases in prokaryotes, methods for the detection and for the production of triacylglycerol lipases, triacylglycerol lipases obtainable thereby, and the use of triacylglycerol lipase-encoding nucleic acids, vectors and recombinant host cells for said methods.
摘要翻译： 本发明涉及编码三酰基甘油脂肪酶的核酸，包含所述核酸的载体，包含所述核酸或载体的宿主细胞，在原核生物中表达三酰甘油脂肪酶的方法，用于检测和产生三酰甘油脂肪酶的方法， 由此获得的三酰基甘油脂酶，以及用于所述方法的用于酰基甘油脂肪酶编码核酸，载体和重组宿主细胞的用途。
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公开(公告)号：US20050048484A1
公开(公告)日：2005-03-03
申请号：US10398178
申请日：2001-10-16
申请人： Bernhard Hauer , Rolf Schmid , Rainer Merkl , Francesca Blasco
发明人： Bernhard Hauer , Rolf Schmid , Rainer Merkl , Francesca Blasco
IPC分类号： C12N15/09 , C12M1/40 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/53 , C12P1/04 , C12P7/00 , C12R1/01 , C12Q1/68 , C07H21/04 , C12N15/74
CPC分类号： C12N9/0077 , C12P1/04
摘要： The invention relates to novel cytochrome P450 monooxygenases from thermophilic bacteria, in particular the genus Thermus sp., to nucleotide sequences encoding them, to the recombinant production of these monooxygenases and to their use for the microbiological oxidation of organic compounds.
摘要翻译： 本发明涉及嗜热细菌，特别是栖热菌属的新型细胞色素P450单加氧酶与编码它们的核苷酸序列，重组生产这些单加氧酶及其用于微生物氧化有机化合物的新型细胞色素P450单加氧酶。
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公开(公告)号：US07364884B2
公开(公告)日：2008-04-29
申请号：US10555719
申请日：2004-05-05
申请人： Bernhard Hauer , Tilo Habicher , Rolf Schmid , Steffen Christian Maurer , Vlada Beniaminovna Urlacher , Holger Schulze , Norbert Huber , Till T. Bachmann
发明人： Bernhard Hauer , Tilo Habicher , Rolf Schmid , Steffen Christian Maurer , Vlada Beniaminovna Urlacher , Holger Schulze , Norbert Huber , Till T. Bachmann
CPC分类号： C12N9/0077
摘要： A process for preparing a hydroxylation catalyst by i) embedding a cystochrome P450 monooxygenase in a sol-gel matrix, ii) embedding an enzymatic NADPH-regenerating system in a sol-gel matrix, and combining the two components i) and ii) unless they were already mixed together before the embedding.
摘要翻译： 一种制备羟基化催化剂的方法，其方法是i）将囊状P450单加氧酶包埋在溶胶 - 凝胶基质中，ii）将酶NADPH再生系统包埋在溶胶 - 凝胶基质中，并将两种组分i）和ii）组合，除非它们 在嵌入之前已经混合在一起。
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8.发明申请Method for the biotransformation of carotenoids by means of a cytochrome p450 monooxygnase 审中-公开通过细胞色素p450单氧化酶生物转化类胡萝卜素的方法公开(公告)号：US20060063226A1
公开(公告)日：2006-03-23
申请号：US10521916
申请日：2003-07-25
申请人： Markus Matuschek , Bernhard Hauer , Rolf Schmid , Isabelle Kauffmann , Francesca Blasco , Claudia Schmidt-Dannert
发明人： Markus Matuschek , Bernhard Hauer , Rolf Schmid , Isabelle Kauffmann , Francesca Blasco , Claudia Schmidt-Dannert
IPC分类号： C12P23/00 , A61K31/12 , A61K31/336
CPC分类号： C12N9/0077 , C12P23/00
摘要： The invention relates to a process for the biotransformation of carotenoids using enzymes having cytochrome P450 monooxygenase activity; in particular monooxygenases from thermophilic bacteria, in particular of the genus Thermus sp.
摘要翻译： 本发明涉及使用具有细胞色素P450单加氧酶活性的酶生物转化类胡萝卜素的方法; 特别是嗜热细菌，特别是嗜热栖热菌属的单加氧酶。
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公开(公告)号：US08206969B2
公开(公告)日：2012-06-26
申请号：US12809118
申请日：2008-12-18
申请人： Bernhard Hauer , Cecilia Kvarnström Branneby , Rolf Schmid , Steffen Maurer , Peter Trodler , Danni Liu , Monika Müller
发明人： Bernhard Hauer , Cecilia Kvarnström Branneby , Rolf Schmid , Steffen Maurer , Peter Trodler , Danni Liu , Monika Müller
IPC分类号： C12N9/84
摘要： A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.
摘要翻译： 具有丙烯酸酯化活性的蛋白质通过引入至少一种下列氨基酸取代：具有SEQ ID NO：1或NO：2的多肽序列：L278A，L278V，W104F，T42A，S47A。
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公开(公告)号：US20100273223A1
公开(公告)日：2010-10-28
申请号：US12809118
申请日：2008-12-18
申请人： Bernhard Hauer , Cecilia Kvarnström Branneby , Rolf Schmid , Steffen Maurer , Peter Trodler , Danni Liu , Monika Müller
发明人： Bernhard Hauer , Cecilia Kvarnström Branneby , Rolf Schmid , Steffen Maurer , Peter Trodler , Danni Liu , Monika Müller
摘要： A protein having acrylation activity having a polypeptide sequence derived from SEQ ID NO: 1 or NO:2 by introducing at least one of the following amino acid substitutions: L278A, L278V, W104F, T42A, S47A.
摘要翻译： 具有丙烯酸酯化活性的蛋白质通过引入至少一种下列氨基酸取代：具有SEQ ID NO：1或NO：2的多肽序列：L278A，L278V，W104F，T42A，S47A。
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