公开(公告)号：US5231005A

公开(公告)日：1993-07-27

申请号：US849481

申请日：1992-03-10

申请人： Thomas Russell ,  Kenneth H. Kortright ,  Wallace H. Coulter ,  Carlos M. Rodriguez ,  Ronald Paul ,  Constance M. Hajek ,  James C. Hudson

发明人： Thomas Russell ,  Kenneth H. Kortright ,  Wallace H. Coulter ,  Carlos M. Rodriguez ,  Ronald Paul ,  Constance M. Hajek ,  James C. Hudson

IPC分类号： G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N35/00

CPC分类号： G01N15/12 ,  G01N33/5002 ,  G01N33/5094 ,  G01N33/5302 ,  G01N33/54326 ,  G01N33/56972 ,  G01N15/14 ,  G01N2015/008 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/1081 ,  G01N35/0098 ,  Y10T436/101666 ,  Y10T436/117497 ,  Y10T436/24

摘要： A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

摘要翻译： 一种用于自动并快速地检索，计数和/或分析全血样本或其部分的至少一个选定细胞群体或形成体，例如白细胞群体及其至少一个子集的方法和装置。 制备含有白细胞的体积的生物培养基，并将至少一种至少一种选择的生物细胞特异性或优先选择的反应物引入其中并在短时间内快速混合。 可以修改细胞的不透明度和/或体积参数，然后在一个或多个步骤中对混合物进行计数和分析，以获得所需的白细胞群体和子集分析。 生物样品可以是全血样品，并且反应物可以包括结合微球的裂解液或单克隆抗体，其将与特定细胞结合，或与结合抗体的溶血和微球体的组合。 微球可以是磁性的，并且结合的细胞可被磁性去除，用于检索和分析剩余的血细胞群体。

公开(公告)号：US4791355A

公开(公告)日：1988-12-13

申请号：US921654

申请日：1986-10-21

申请人： Wallace H. Coulter ,  Carlos M. Rodriguez

发明人： Wallace H. Coulter ,  Carlos M. Rodriguez

IPC分类号： G01N15/12 ,  G01N33/48 ,  G01N27/00

CPC分类号： G01N15/1227 ,  G01N2015/129

摘要： A circuit, which is useful to provide data needed to measure the electrical opacity of a particle, for instance a blood cell, passing through a Coulter type transducer, includes a current source for providing a conventional d.c. current through the sensing aperture of the transducer, as well as an oscillator for providing a high frequency current through the aperture. The oscillator includes an active device and a resonant circuit, and the aperture is coupled in parallel with the resonant circuit of the oscillator. The oscillator can be any type of oscillator, such as a Hartley oscillator. As a particle passes through the aperture, the resistance of the aperture increases, which in turn, increases the Q of the oscillator circuit, whereby the oscillator output signal is amplitude modulated in accordance with the increased Q. This amplitude modulated signal can be detected to provide a value based on the high frequency reactance of the particle being detected. The change in d.c. resistance due to a particle passing through the aperture is detected in a conventional manner. The detected amplitudes of the a.c. and d.c. signals then can be compared to determine the opacity of each particle. Multiple oscillators of different frequencies can be coupled in parallel with the transducer through a coupling circuit, which connects each oscillator to the transducer through a low impedance path and isolates each oscillator from signals having frequencies of the other oscillators.

摘要翻译： 用于提供测量通过Coulter型换能器的颗粒（例如血细胞）的电气不透明度所需的数据的电路包括用于提供常规直流电流的电流源。 通过传感器的感测孔径的电流，以及用于提供通过孔径的高频电流的振荡器。 振荡器包括有源器件和谐振电路，并且该孔与振荡器的谐振电路并联耦合。 振荡器可以是任何类型的振荡器，例如Hartley振荡器。 当颗粒通过孔径时，孔径的电阻增加，这又增加了振荡器电路的Q，由此根据增加的Q对振荡器输出信号进行幅度调制。该幅度调制信号可以被检测为 提供基于所检测的粒子的高频电抗的值。 d.c.的变化 以常规方式检测通过孔径的颗粒的电阻。 检测到的a.c.的振幅。 和d.c. 然后可以比较信号来确定每个粒子的不透明度。 不同频率的多个振荡器可以通过耦合电路与传感器并联耦合，该耦合电路通过低阻抗路径将每个振荡器连接到换能器，并将每个振荡器与具有其他振荡器频率的信号隔离开来。

公开(公告)号：US06579685B1

公开(公告)日：2003-06-17

申请号：US08303924

申请日：1994-09-09

申请人： Thomas Russell ,  James Carey Hudson ,  Wallace H. Coulter ,  Carlos M. Rodriguez ,  Constance Mary Hajek

发明人： Thomas Russell ,  James Carey Hudson ,  Wallace H. Coulter ,  Carlos M. Rodriguez ,  Constance Mary Hajek

IPC分类号： G01N3353

CPC分类号： G01N33/56972 ,  Y10S435/808 ,  Y10S435/962 ,  Y10S436/805 ,  Y10S436/824 ,  Y10S436/825

摘要： A method for performing screening of one or more cell groups of interest obscured by a cell population such as one or more subsets of interest of a WBC population utilizing at least one light sensing parameter. The cell group of interest is enumerated by utilizing microspheres having monoclonal antibodies bound thereto to modify the sensed characteristics of specified cells to differentiate the cell group of interest from the obscuring cell population.

摘要翻译： 利用至少一个光感测参数，用于进行筛选感兴趣的一个或多个感兴趣的细胞群的方法被细胞群体例如WBC群体的一个或多个感兴趣的亚群遮蔽。 通过利用与其结合的具有单克隆抗体的微球来修饰感兴趣的特定细胞的特征以区分感兴趣的细胞群和模糊细胞群体，来计算感兴趣的细胞群。

公开(公告)号：US6159740A

公开(公告)日：2000-12-12

申请号：US525231

申请日：1990-05-17

申请人： James Carey Hudson ,  Thomas Russell ,  Carlos M. Rodriguez ,  Wallace H. Coulter

发明人： James Carey Hudson ,  Thomas Russell ,  Carlos M. Rodriguez ,  Wallace H. Coulter

IPC分类号： C12M1/34 ,  G01N1/28 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/577 ,  G01N35/00 ,  G01N33/80

CPC分类号： G01N1/28 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/5094 ,  G01N33/5302 ,  G01N33/54326 ,  G01N33/56972 ,  G01N35/0098 ,  G01N2015/008 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/1081 ,  G01N2015/1486 ,  G01N2015/149

摘要： A method and apparatus for performing screening of obscured or partially obscured cells to enumerate one or more cell population subsets. For example, a whole blood sample or portion thereof is screened to provide the desired analysis of one or more white blood cell population subsets in the sample. A white blood cell population including the obscured subset of interest is first counted, along with a standard population. The standard population can be one of the total number of white blood cell populations, a second white blood cell population which does not obscure the shifted or non-shifted sensed characteristic of the subset of interest, an artificial population formed by microspheres which also do not obscure the shifted or non-shifted sensed characteristic of the subset of interest or a white blood cell population into which the sensed characteristic of the subset will be wholly or partially shifted. The sensed characteristic of the white blood cell population subset of interest then is shifted by binding microspheres having monoclonal antibodies specific to the white blood cell population subset of interest to the cell population. The white blood cell population, and the standard population then are again counted and compared to the original counts to obtain an enumeration of the white blood cell population subset of interest.

摘要翻译： 用于执行遮蔽或部分模糊的细胞的筛选以枚举一个或多个细胞群体亚群的方法和装置。 例如，筛选全血样品或其一部分以提供样品中一种或多种白细胞群体亚群的期望分析。 首先计算包括所隐藏的感兴趣亚群的白细胞群，以及标准群体。 标准群体可以是白细胞群体总数中的一个，第二白血球群体不掩盖感兴趣亚群的移位或非移位感知特征，由微球形成的人造群体也不 模糊感兴趣子集的移位或未移位的感测特征或者该子集的感测特征将被完全或部分移位到其中的白细胞群。 感兴趣的白血细胞群体子集的感测特征然后通过将具有特异于感兴趣的白细胞群体子集的单克隆抗体的微球结合到细胞群体来移位。 然后再次对白细胞群体和标准群体进行计数，并将其与原始计数进行比较，以获得感兴趣的白细胞群体子集的计数。

公开(公告)号：US5260192A

公开(公告)日：1993-11-09

申请号：US929156

申请日：1992-08-12

申请人： Thomas Russell ,  Constance M. Hajek ,  Carlos M. Rodriguez ,  Wallace H. Coulter

发明人： Thomas Russell ,  Constance M. Hajek ,  Carlos M. Rodriguez ,  Wallace H. Coulter

IPC分类号： G01N1/28 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N35/00 ,  C12Q1/00 ,  C12Q1/04 ,  C12Q1/06 ,  C12Q1/24

CPC分类号： G01N15/10 ,  G01N1/28 ,  G01N15/12 ,  G01N33/5094 ,  G01N33/5302 ,  G01N33/54326 ,  G01N33/56972 ,  G01N15/14 ,  G01N2015/008 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/1081 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N35/0098 ,  Y10T436/101666 ,  Y10T436/117497

摘要： A method and apparatus for automatically and rapidly, retrieving counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. A multipart blood cell analysis is obtained with a single sensing parameter by depleting at least one WBC subset population. The percentage of a desired WBC population subset or the overlapping of WBC subset populations also can be obtained by subtracting one or more obscuring WBC subset populations.The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

摘要翻译： 一种用于自动和快速地检索计数和/或分析全血样品或其部分的至少一个选定细胞群体或形成体（例如白细胞群体及其至少一个子集）的方法和装置。 制备含有白细胞的体积的生物培养基，并将至少一种至少一种选择的生物细胞特异性或优先选择的反应物引入其中并在短时间内快速混合。 通过消耗至少一个WBC子群体，通过单个感测参数获得多部分血细胞分析。 通过减去一个或多个模糊的WBC子集群，可以获得期望的WBC群体子集的百分比或WBC子群体的重叠。 生物样品可以是全血样品，并且反应物可以包括结合微球的裂解液或单克隆抗体，其将与特定细胞结合，或与结合抗体的溶血和微球体的组合。 微球可以是磁性的，并且结合的细胞可被磁性去除，用于检索和分析剩余的血细胞群体。

公开(公告)号：US5125737A

公开(公告)日：1992-06-30

申请号：US479199

申请日：1990-02-13

申请人： Carlos M. Rodriguez ,  Wallace H. Coulter

发明人： Carlos M. Rodriguez ,  Wallace H. Coulter

IPC分类号： G01N33/49 ,  G01N1/38 ,  G01N15/00 ,  G01N15/10 ,  G01N15/14 ,  G01N21/53

CPC分类号： G01N15/1459 ,  G01N21/53 ,  G01N1/38 ,  G01N2015/008 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/129 ,  G01N2015/1413 ,  G01N2015/1477 ,  G01N2021/4711

摘要： Disclosed is a flow through particle analyzing cell differentiating apparatus for optical and electronic measurements on a stream of particles in which a hydrodynamically focoussed stream is passed into and through a point focussed beam of radiated energy whereby the beam is scattered by the stream to impinge upon light responsive signal operating members disposed adjacent the cell at angular positions relative to the beam axis of from about 10.degree. to 70.degree..

摘要翻译： 公开了一种用于在粒子流上进行光学和电子测量的流动粒子分析单元分辨装置，其中流体动力聚焦的流流入并通过点聚焦的辐射能量束，由此光束被流散射以照射到光 响应信号操作构件相对于梁轴线在约10°至70°的角度位置处邻近电池设置。

公开(公告)号：US20090035731A1

公开(公告)日：2009-02-05

申请号：US12189449

申请日：2008-08-11

申请人： Patrick Murphy ,  Todd Conard ,  Waldemar H. Tunkis ,  Michael Goldenberg ,  Carlos M. Rodriguez

发明人： Patrick Murphy ,  Todd Conard ,  Waldemar H. Tunkis ,  Michael Goldenberg ,  Carlos M. Rodriguez

IPC分类号： G09B21/00

CPC分类号： G09B21/003

摘要： The present invention discloses an electromechanical tactile cell assembly comprising a plurality of piezoelectric element reeds, each one of the piezoelectric element reeds being bendable at an elongated end portion when a voltage is applied to the reed, a plurality of conductive fulcrum pins secured to a printed circuit board, and a plurality of multiple element conductive supports secured to a printed circuit board, each multiple element conductive support, in combination with the plurality of conductive fulcrum pins, adapted to secure a plurality of piezoelectric reeds, corresponding to the plurality of conductive fulcrum pins, to the printed circuit board. The electromechanical tactile cell is adaptable for use with a Braille display.

摘要翻译： 本发明公开了一种机电触觉电池组件，其包括多个压电元件簧片，当将电压施加到所述簧片时，所述压电元件簧片中的每一个可在细长端部处弯曲，多个导电支点销固定到印刷 电路板和固定到印刷电路板的多个多元件导电支撑件，每个多个元件导电支撑件与多个导电支点销组合，适于固定多个对应于多个导电支点的压电簧片 引脚到印刷电路板。 机电触觉电池适用于盲文显示屏。

公开(公告)号：US08360778B2

公开(公告)日：2013-01-29

申请号：US12645654

申请日：2009-12-23

申请人： Patrick Murphy ,  Todd Conard ,  Waldemar H. Tunkis ,  Michael Goldenberg ,  Carlos M. Rodriguez

发明人： Patrick Murphy ,  Todd Conard ,  Waldemar H. Tunkis ,  Michael Goldenberg ,  Carlos M. Rodriguez

IPC分类号： G09B21/00

CPC分类号： G09B21/004

摘要： An electromechanical Braille cell assembly includes a plurality of parallel bimorph reeds. A first plurality of bimorph reeds is mounted to a first side of a printed circuit board by a first plurality of clips and a second plurality of bimorph reeds is mounted to a second side of the printed circuit board by a second plurality of clips. A frame has a top wall, a bottom wall, a first side wall in the form of an angle wall and a second side wall in the form of a flat wall. Pinholes are formed in the angle wall to accommodate Braille pins and the flat wall is slotted to accommodate a plurality of printed circuit boards. A monolithic cap covers all of the Braille pins. Pinholes formed in the cap receive the respective tips of the Braille cells when actuated bimorph reeds cause displacement of the Braille pins.

摘要翻译： 机电盲孔单元组件包括多个平行的双压电晶片。 第一多个双压电晶片通过第一多个夹子安装到印刷电路板的第一侧，并且第二多个双压电晶片通过第二多个夹子安装到印刷电路板的第二侧。 框架具有顶壁，底壁，呈角壁形式的第一侧壁和呈平壁形式的第二侧壁。 针孔形成在角壁中以适应盲文销，并且平壁开槽以容纳多个印刷电路板。 单片盖覆盖所有盲文引脚。 当致动的双压电晶片引起盲文引脚的位移时，形成在帽中的针孔接收盲文单元的各个尖端。

公开(公告)号：US20120307026A1

公开(公告)日：2012-12-06

申请号：US13584204

申请日：2012-08-13

申请人： Carlos M. Rodriguez ,  Patrick Murphy ,  Waldemar Tunkis ,  Todd Conard ,  Michael Goldenberg ,  Jeffrey McDaniel

发明人： Carlos M. Rodriguez ,  Patrick Murphy ,  Waldemar Tunkis ,  Todd Conard ,  Michael Goldenberg ,  Jeffrey McDaniel

IPC分类号： H04N7/18

CPC分类号： H04N5/23293 ,  G03B17/563 ,  H04N1/00129 ,  H04N5/2252 ,  H04N5/23296 ,  H04N5/2628 ,  H04N9/64 ,  H04N2101/00

摘要： Disclosed is a magnifier for use by blind or low vision users. The magnifier includes a camera, such as a CMOS image sensor, that displays enlarged images upon a screen for easy viewing. The magnifier further includes a handle that is pivotally interconnected to a housing to allow for handheld use in a variety of different configurations.

摘要翻译： 公开了一种用于盲人或低视力用户使用的放大镜。 放大镜包括诸如CMOS图像传感器的相机，其在屏幕上显示放大的图像以便于观看。 放大镜还包括手柄，该手柄枢转地互连到壳体以允许以各种不同构造的手持使用。

公开(公告)号：US07929013B2

公开(公告)日：2011-04-19

申请号：US11379327

申请日：2006-04-19

申请人： Michael Goldenberg ,  Bradley S. Davis ,  Lee Hamilton ,  Carlos M. Rodriguez ,  Patrick Murphy ,  Waldemar H. Tunkis

发明人： Michael Goldenberg ,  Bradley S. Davis ,  Lee Hamilton ,  Carlos M. Rodriguez ,  Patrick Murphy ,  Waldemar H. Tunkis

IPC分类号： H04N7/18

CPC分类号： H04N5/2251 ,  G02B27/026 ,  G02B27/027 ,  G03B27/323 ,  G06F1/1607 ,  H04N5/232 ,  H04N5/23293 ,  H04N5/247 ,  H04N5/2628

摘要： A desktop electronic magnifier in a first embodiment includes a monitor and a control panel mounted to the monitor so that access to the control panel cannot be blocked by any position of the monitor. In a second embodiment, a monitor is positioned atop a flat platform that surmounts the camera so that access to the control panel cannot be blocked by any position of the monitor. In a third embodiment, the control panel is mounted to the camera and the monitor is supported by a support surface independent of the magnifier. Access to the control panel is blocked only if the user positions the monitor in blocking relation to the control panel.

摘要翻译： 在第一实施例中的台式电子放大镜包括监视器和安装到监视器的控制面板，使得对控制面板的访问不能被监视器的任何位置阻挡。 在第二实施例中，监视器位于平坦平台的顶部，其超越照相机，使得对控制面板的访问不能被监视器的任何位置阻挡。 在第三实施例中，控制面板安装到照相机，监视器由与放大镜无关的支撑表面支撑。 只有当用户将显示器与控制面板保持关联的位置时，控制面板的访问才会被阻止。