利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

31 条记录 (耗时 0.02 秒)

    Particle analyzer for measuring the resistance and reactance of a particle
    2.
    发明授权
    Particle analyzer for measuring the resistance and reactance of a particle 失效
    用于测量颗粒的电阻和电抗的粒子分析仪

    公开(公告)号：US4791355A

    公开(公告)日：1988-12-13

    申请号：US921654

    申请日：1986-10-21

    申请人： Wallace H. Coulter ,  Carlos M. Rodriguez

    发明人： Wallace H. Coulter ,  Carlos M. Rodriguez

    IPC分类号： G01N15/12 ,  G01N33/48 ,  G01N27/00

    CPC分类号： G01N15/1227 ,  G01N2015/129

    摘要： A circuit, which is useful to provide data needed to measure the electrical opacity of a particle, for instance a blood cell, passing through a Coulter type transducer, includes a current source for providing a conventional d.c. current through the sensing aperture of the transducer, as well as an oscillator for providing a high frequency current through the aperture. The oscillator includes an active device and a resonant circuit, and the aperture is coupled in parallel with the resonant circuit of the oscillator. The oscillator can be any type of oscillator, such as a Hartley oscillator. As a particle passes through the aperture, the resistance of the aperture increases, which in turn, increases the Q of the oscillator circuit, whereby the oscillator output signal is amplitude modulated in accordance with the increased Q. This amplitude modulated signal can be detected to provide a value based on the high frequency reactance of the particle being detected. The change in d.c. resistance due to a particle passing through the aperture is detected in a conventional manner. The detected amplitudes of the a.c. and d.c. signals then can be compared to determine the opacity of each particle. Multiple oscillators of different frequencies can be coupled in parallel with the transducer through a coupling circuit, which connects each oscillator to the transducer through a low impedance path and isolates each oscillator from signals having frequencies of the other oscillators.

    摘要翻译： 用于提供测量通过Coulter型换能器的颗粒（例如血细胞）的电气不透明度所需的数据的电路包括用于提供常规直流电流的电流源。 通过传感器的感测孔径的电流，以及用于提供通过孔径的高频电流的振荡器。 振荡器包括有源器件和谐振电路，并且该孔与振荡器的谐振电路并联耦合。 振荡器可以是任何类型的振荡器，例如Hartley振荡器。 当颗粒通过孔径时，孔径的电阻增加，这又增加了振荡器电路的Q，由此根据增加的Q对振荡器输出信号进行幅度调制。该幅度调制信号可以被检测为 提供基于所检测的粒子的高频电抗的值。 d.c.的变化 以常规方式检测通过孔径的颗粒的电阻。 检测到的a.c.的振幅。 和d.c. 然后可以比较信号来确定每个粒子的不透明度。 不同频率的多个振荡器可以通过耦合电路与传感器并联耦合，该耦合电路通过低阻抗路径将每个振荡器连接到换能器，并将每个振荡器与具有其他振荡器频率的信号隔离开来。

    Method and apparatus for screening obscured or partially obscured cells
    4.
    发明授权
    Method and apparatus for screening obscured or partially obscured cells 失效
    用于筛选模糊或部分模糊的细胞的方法和装置

    公开(公告)号：US6159740A

    公开(公告)日：2000-12-12

    申请号：US525231

    申请日：1990-05-17

    申请人： James Carey Hudson ,  Thomas Russell ,  Carlos M. Rodriguez ,  Wallace H. Coulter

    发明人： James Carey Hudson ,  Thomas Russell ,  Carlos M. Rodriguez ,  Wallace H. Coulter

    IPC分类号： C12M1/34 ,  G01N1/28 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569 ,  G01N33/577 ,  G01N35/00 ,  G01N33/80

    CPC分类号： G01N1/28 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14 ,  G01N33/5094 ,  G01N33/5302 ,  G01N33/54326 ,  G01N33/56972 ,  G01N35/0098 ,  G01N2015/008 ,  G01N2015/1037 ,  G01N2015/1062 ,  G01N2015/1081 ,  G01N2015/1486 ,  G01N2015/149

    摘要： A method and apparatus for performing screening of obscured or partially obscured cells to enumerate one or more cell population subsets. For example, a whole blood sample or portion thereof is screened to provide the desired analysis of one or more white blood cell population subsets in the sample. A white blood cell population including the obscured subset of interest is first counted, along with a standard population. The standard population can be one of the total number of white blood cell populations, a second white blood cell population which does not obscure the shifted or non-shifted sensed characteristic of the subset of interest, an artificial population formed by microspheres which also do not obscure the shifted or non-shifted sensed characteristic of the subset of interest or a white blood cell population into which the sensed characteristic of the subset will be wholly or partially shifted. The sensed characteristic of the white blood cell population subset of interest then is shifted by binding microspheres having monoclonal antibodies specific to the white blood cell population subset of interest to the cell population. The white blood cell population, and the standard population then are again counted and compared to the original counts to obtain an enumeration of the white blood cell population subset of interest.

    摘要翻译： 用于执行遮蔽或部分模糊的细胞的筛选以枚举一个或多个细胞群体亚群的方法和装置。 例如，筛选全血样品或其一部分以提供样品中一种或多种白细胞群体亚群的期望分析。 首先计算包括所隐藏的感兴趣亚群的白细胞群，以及标准群体。 标准群体可以是白细胞群体总数中的一个，第二白血球群体不掩盖感兴趣亚群的移位或非移位感知特征，由微球形成的人造群体也不 模糊感兴趣子集的移位或未移位的感测特征或者该子集的感测特征将被完全或部分移位到其中的白细胞群。 感兴趣的白血细胞群体子集的感测特征然后通过将具有特异于感兴趣的白细胞群体子集的单克隆抗体的微球结合到细胞群体来移位。 然后再次对白细胞群体和标准群体进行计数，并将其与原始计数进行比较，以获得感兴趣的白细胞群体子集的计数。

    Closed vial transfer method and system
    7.
    发明授权
    Closed vial transfer method and system 失效
    封闭瓶子转移方法和系统

    公开(公告)号：US5653686A

    公开(公告)日：1997-08-05

    申请号：US372196

    申请日：1995-01-13

    申请人： Wallace H. Coulter ,  Charles R. Shambaugh

    发明人： Wallace H. Coulter ,  Charles R. Shambaugh

    IPC分类号： A61J1/00 ,  A61J1/20 ,  B01L3/14 ,  G01N1/00 ,  A61M31/00

    CPC分类号： G01N35/1079 ,  A61J1/2089 ,  B01L3/5082 ,  A61J1/201 ,  A61J1/2017 ,  A61J1/2058 ,  A61J1/2072 ,  A61J1/2075 ,  A61J1/2082 ,  A61J1/2086 ,  A61J1/2096

    摘要： A method and transfer device for transferring fluids between closed vials. The transfer device includes a cylinder having a pair of opposing open tubes or tubular portions into which a pair of vials can be inserted to transfer fluid therebetween. The tubes are separated by a wall through which extends a fluid transfer needle and a vent needle which first is inserted into a first vial to vent the first vial into a vent chamber or reservoir formed in the transfer device before the transfer needle is inserted into the first vial. The first vial, stopper first, is inserted into first tube until seated onto an exposed end of the transfer needle. A second vial, stopper first, then is inserted into the second tube until seated onto the opposite exposed end of the transfer needle. A pressure differential between the closed vials preferably can be utilized to transfer a desired fluid portion between the vials.

    摘要翻译： 一种用于在密封小瓶之间传送流体的方法和传送装置。 转移装置包括具有一对相对的开放管或管状部分的圆柱体，一对小瓶可插入其中以在其间传送流体。 管被壁分开，通过该壁将流体输送针和通气针延伸，首先将其插入第一小瓶中，以将第一小瓶排出到形成在转移装置中的通气室或储存器中，然后将输送针插入 第一个小瓶。 首先将第一个小瓶，塞子插入第一个管子，直到坐到转移针的暴露端上。 首先将第二个小瓶，塞子插入第二个管子中，直到安置在转移针的相对的暴露端上。 密封小瓶之间的压力差优选地可用于在小瓶之间转移期望的流体部分。

    Particle analyzer of the Coulter type including coincidence error correction circuitry
    8.
    发明授权
    Particle analyzer of the Coulter type including coincidence error correction circuitry 失效
    Coulter型粒子分析仪包括重合误差校正电路

    公开(公告)号：US3968429A

    公开(公告)日：1976-07-06

    申请号：US594209

    申请日：1975-07-09

    申请人： Wallace H. Coulter ,  Walter R. Hogg

    发明人： Wallace H. Coulter ,  Walter R. Hogg

    IPC分类号： G01N15/12 ,  G01N27/00

    CPC分类号： G01N15/1245 ,  G01N15/1227

    摘要： A particle analyzer of the Coulter type has at least first and second sensing zone arrangements, each having substantially the same dimensions and each generating a train of particle pulses in response to passage through the respective sensing zone of a sample containing a plurality of particles to be counted. A summing circuit connected to the output of each sensing zone sums together each of the trains of particle pulses to develop a summed train of pulses. Additional circuitry is connected to each sensing zone and the summing circuitry. This circuitry is operative to change mathematically at least the number of particle pulses in the summed train of particle pulses. The mathematically changed summed train of pulses and the train of pulses from the first and second sensing zones then are accumulated to produce an error corrected particle pulse count.

    摘要翻译： 库尔特型的粒子分析仪具有至少第一和第二感测区域布置，每个具有基本上相同的尺寸，并且每个产生一系列粒子脉冲以响应于通过包含多个颗粒的样品的相应感测区域而成 算了 连接到每个感测区域的输出的求和电路将每个粒子脉冲列组合在一起，以产生一个总和的脉冲序列。 附加电路连接到每个感测区域和求和电路。 该电路用于在数学上改变粒子脉冲的总和列中的至少粒子脉冲的数量。 然后累积数学改变的脉冲序列和来自第一和第二感测区域的脉冲串，以产生误差校正的粒子脉冲计数。

    Method of selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation
    9.
    发明授权
    Method of selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation 失效
    选择使用粒子和重力沉降的样品群体或亚群体的方法

    公开(公告)号：US06900029B1

    公开(公告)日：2005-05-31

    申请号：US08556667

    申请日：1995-11-13

    申请人： Wallace H. Coulter ,  Robert K. Zwerner ,  Robert J. Schmittling ,  Thomas R. Russell

    发明人： Wallace H. Coulter ,  Robert K. Zwerner ,  Robert J. Schmittling ,  Thomas R. Russell

    IPC分类号： B01D21/00 ,  G01N33/543 ,  G01N33/553 ,  G01N33/569 ,  G01N33/574 ,  A01N1/02 ,  C12Q1/18 ,  G01N33/53

    CPC分类号： G01N33/54333 ,  G01N33/553 ,  G01N33/56972 ,  G01N33/574 ,  Y10S436/824

    摘要： A separation procedure for separating a selected desired or undesired population from a biological sample utilizing relatively heavy, dense particles and gravity sedimentation. The particles have one or more reactants bound thereto which are specific to and will bind with the selected population. The particles preferably are mixed with the sample by repeatedly causing the particles to settle through a substantial portion of the sample to bind to the selected population. The particles with the bound selected population then are allowed to preferentially settle in the sample and the supernatant including an enriched population is separated from the particles with the selected population bound thereto. The enriched populations in the biological sample supernatant can be further enriched by multiple removal steps.

    摘要翻译： 用于使用相对重的，致密的颗粒和重力沉降从生物样品中分离所选择的所需或不需要的群体的分离程序。 颗粒具有一个或多个与其结合的反应物，其特异于并将与所选群体结合。 颗粒优选通过重复使颗粒沉淀通过样品的大部分与样品结合而与样品混合。 然后允许具有结合的所选群体的颗粒优先沉降在样品中，并且将包含富集群体的上清液从结合有所选群体的颗粒中分离。 通过多次去除步骤，可以进一步富集生物样品上清液中的富集群体。