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公开(公告)号:US20100136529A1
公开(公告)日:2010-06-03
申请号:US12230628
申请日:2008-09-02
申请人: Daniel Shoemaker , Martin Fuchs , Neil X. Krueger , Mehmet Toner , Darren Gray , Ravi Kapur , Zihua Wang
发明人: Daniel Shoemaker , Martin Fuchs , Neil X. Krueger , Mehmet Toner , Darren Gray , Ravi Kapur , Zihua Wang
CPC分类号: C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
摘要: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
摘要翻译: 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常的系统,装置和方法,例如, 非整倍体 本发明涉及用不同的标记标记所述混合样品中每个细胞中的基因组DNA的区域,其中每个标记对每个细胞是特异性的,并且定量混合样品中每个细胞的基因组DNA的标记区域。 更具体地,本发明涉及从混合样品中的每个细胞定量标记的DNA多态性。
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公开(公告)号:US20080220422A1
公开(公告)日:2008-09-11
申请号:US11763421
申请日:2007-06-14
申请人: Daniel Shoemaker , Martin Fuchs , Neil X. Krueger , Mehmet Toner , Darren Gray , Ravi Kapur , Zihua Wang
发明人: Daniel Shoemaker , Martin Fuchs , Neil X. Krueger , Mehmet Toner , Darren Gray , Ravi Kapur , Zihua Wang
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6881 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6883 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
摘要: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
摘要翻译: 本发明提供了当与样品中的母体细胞群混合时检测胎儿细胞的存在并测试胎儿异常的系统,装置和方法,例如, 非整倍体 本发明涉及用不同的标记标记所述混合样品中每个细胞中的基因组DNA的区域,其中每个标记对每个细胞是特异性的,并且定量混合样品中每个细胞的基因组DNA的标记区域。 更具体地,本发明涉及从混合样品中的每个细胞定量标记的DNA多态性。
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公开(公告)号:US20120171666A1
公开(公告)日:2012-07-05
申请号:US13306640
申请日:2011-11-29
申请人: Daniel Shoemaker , Martin Fuchs , Neil X. Krueger , Mehmet Toner , Darren Gray , Ravl Kapur , Zihua Wang
发明人: Daniel Shoemaker , Martin Fuchs , Neil X. Krueger , Mehmet Toner , Darren Gray , Ravl Kapur , Zihua Wang
IPC分类号: G01N27/62
CPC分类号: C12Q1/6883 , B01L3/502761 , C12Q1/6809 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/158 , C12Q2600/16 , G01N2015/1006 , G01N2015/1087 , Y10T436/143333
摘要: The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.
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公开(公告)号:US20080090239A1
公开(公告)日:2008-04-17
申请号:US11762747
申请日:2007-06-13
申请人: DANIEL SHOEMAKER , Martin Fuchs , Neil Krueger , Mehmet Toner , Darren Gray , Ravi Kapur , Zihua Wang
发明人: DANIEL SHOEMAKER , Martin Fuchs , Neil Krueger , Mehmet Toner , Darren Gray , Ravi Kapur , Zihua Wang
CPC分类号: C12Q1/6886 , C12Q2600/156 , G01N1/405 , G01N33/5005 , G01N33/5091 , G01N33/57484 , G01N2015/1006 , G01N2015/1087 , G01N2800/385
摘要: Described herein are methods to diagnose or prognose cancer in a subject by enriching, detecting, and analyzing individual rare cells, e.g., epithelial cells, in a sample from the subject. Also described are methods for labeling regions of genomic DNA in individual cells in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the method includes detecting the presence of gene mutations in individual rare cells in a subsample.
摘要翻译: 本文描述的是通过在受试者的样品中富集,检测和分析个体稀有细胞(例如上皮细胞)来诊断或预测受试者的癌症的方法。 还描述了用于在具有不同标记的所述混合样品中标记个别细胞中基因组DNA区域的方法,其中每个标记对于每个细胞是特异性的,并且定量来自混合样品中每个细胞的基因组DNA的标记区域。 更具体地,该方法包括检测子样品中个体稀有细胞中基因突变的存在。
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公开(公告)号:US20070059781A1
公开(公告)日:2007-03-15
申请号:US11229336
申请日:2005-09-15
申请人: Ravi Kapur , Mehmet Toner , Lotion Huang , Tom Barber , Bruce Carvalho , Darren Gray
发明人: Ravi Kapur , Mehmet Toner , Lotion Huang , Tom Barber , Bruce Carvalho , Darren Gray
IPC分类号: G01N33/567 , G06F19/00 , C12M3/00
CPC分类号: B01L3/502753 , B01L3/502746 , B01L2200/0647 , B01L2300/0816 , B01L2300/0864 , B01L2400/0409 , B01L2400/0472 , B01L2400/086 , B82Y15/00 , B82Y30/00 , G01N1/40 , G01N2035/00237
摘要: The invention relates to one or more size-based separation modules adapted to increase a concentration of a first analyte in a sample by at least 10,000 fold, wherein said first analyte has an initial concentration in said sample of less than 1×10−3 analytes/μL, and an analyzer for analyzing said first analytes in an enriched medium.
摘要翻译: 本发明涉及一种或多种基于尺寸的分离模块,其适于将样品中的第一分析物的浓度增加至少10,000倍,其中所述第一分析物在所述样品中的初始浓度小于1×10 -3 分析物/ muL,以及用于分析富集介质中的所述第一分析物的分析器。
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公开(公告)号:US20050282293A1
公开(公告)日:2005-12-22
申请号:US11071270
申请日:2005-03-03
申请人: Maury Cosman , Ravi Kapur , Bruce Carvalho , Tom Barber , Ulysses Balis , Mehmet Toner , Lotien Huang , Darren Gray
发明人: Maury Cosman , Ravi Kapur , Bruce Carvalho , Tom Barber , Ulysses Balis , Mehmet Toner , Lotien Huang , Darren Gray
CPC分类号: G01N1/14 , B01L3/5027 , B01L3/50825 , B01L2200/026 , G01N1/38 , G01N35/1095 , Y10T436/2575
摘要: The invention features methods and devices for the delivery of a fluid medium containing analytes, e.g., particles, solutes, or solvents, to an analytical device. The systems are designed to minimize contact with potentially hazardous, fragile, or valuable samples. The systems allow for the dilution, mixing, and introduction of the fluid medium to an analytical device, followed by possible further analysis or sample manipulation.
摘要翻译: 本发明的特征在于将含有分析物例如颗粒,溶质或溶剂的流体介质输送到分析装置的方法和装置。 这些系统旨在最大程度地减少与潜在的危险,易碎或有价值的样品的接触。 该系统允许将流体介质稀释,混合和引入分析装置,随后进行可能的进一步分析或样品操作。
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公开(公告)号:US20070059683A1
公开(公告)日:2007-03-15
申请号:US11229359
申请日:2005-09-15
申请人: Tom Barber , Lotien Huang , Darren Gray , Ravi Kapur
发明人: Tom Barber , Lotien Huang , Darren Gray , Ravi Kapur
IPC分类号: C12Q1/70 , C12Q1/68 , G01N33/567 , G01N33/53
CPC分类号: C12Q1/24 , B82Y15/00 , B82Y30/00 , G01N33/5002 , G01N33/5091
摘要: The invention relates to a method for diagnosing an animal for a condition by obtaining a fluid sample from the animal, enriching a first analyte having a concentration of less than 1×10−3 analytes/μL from said sample by a factor of at least 10,000 fold; and analyzing one or more enriched first analytes to determine a condition in said animal. Enrichment is preferably performed using one or more size-based separation modules.
摘要翻译: 本发明涉及一种用于通过从动物获得流体样品来诊断动物的方法,所述方法从所述样品富集浓度低于1×10 -3分析物/μL的第一分析物, 至少10,000倍的因子; 以及分析一种或多种富集的第一分析物以确定所述动物的状况。 富集优选使用一个或多个基于尺寸的分离模块进行。
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公开(公告)号:US20110272282A1
公开(公告)日:2011-11-10
申请号:US13128387
申请日:2009-11-10
申请人: Andrea Marziali , David John Broemeling , Joel Pel , Jason Donald Thompson , Jaryn Perkins , Thomas Willis , Herbert Heyneker , Darren Gray , Carolina Tropini
发明人: Andrea Marziali , David John Broemeling , Joel Pel , Jason Donald Thompson , Jaryn Perkins , Thomas Willis , Herbert Heyneker , Darren Gray , Carolina Tropini
CPC分类号: C07K1/24 , B01D57/02 , C12N15/101 , C12Q1/6806 , C12Q2565/125 , C12Q2525/125
摘要: Methods and apparatus for concentrating particles may be applied, for example, to concentrating DNA, RNA, proteins and the like. Proteins may be pre-treated to facilitate concentration by scodaphoresis. The pre-treatment may comprise, for example, heating or chemical treatment to denature and/or apply a net charge to the protein, binding handle particles to the protein and combinations thereof. High-conductivity samples may be subjected to a conductivity-reduction step to facilitate electrical injection of target particles into scodaphoresis media. The conductivity-reduction step may comprise a buffer exchange process or a salt extraction process, for example. Methods and apparatus can allow two or more different types of target particles to be extracted from the same sample and separately concentrated. These various aspects may be applied individually or in any combination.
摘要翻译: 用于浓缩颗粒的方法和装置可以用于例如浓缩DNA,RNA,蛋白质等。 可以预先处理蛋白质以促进通过scopaphoresis的浓缩。 预处理可以包括例如加热或化学处理以使蛋白质变性和/或施加净电荷,将手段颗粒结合到蛋白质及其组合。 可以对高电导率样品进行电导率降低步骤,以便于将目标颗粒电注射到电泳培养基中。 导电性降低步骤可以包括例如缓冲液交换过程或盐提取过程。 方法和装置可以允许从相同样品中提取两种或更多种不同类型的靶颗粒,并分别浓缩。 这些各个方面可以单独地或以任何组合应用。
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公开(公告)号:US20090071831A1
公开(公告)日:2009-03-19
申请号:US10587817
申请日:2005-02-04
申请人: Christopher S. Chen , Darren Gray , John Tan , Joel Voldman
发明人: Christopher S. Chen , Darren Gray , John Tan , Joel Voldman
CPC分类号: G01N27/447
摘要: The invention provides apparatus and methods for arraying particles on a surface using dielectrophoresis.
摘要翻译: 本发明提供了使用介电电泳在颗粒表面上排列颗粒的装置和方法。
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