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    Fluorescence standard, and the use thereof
    3.
    发明授权
    Fluorescence standard, and the use thereof 有权
    荧光标准及其用途

    公开(公告)号：US08704158B2

    公开(公告)日：2014-04-22

    申请号：US12937009

    申请日：2009-04-17

    申请人： Klaus Haberstroh ,  Konrad Faulstich

    发明人： Klaus Haberstroh ,  Konrad Faulstich

    IPC分类号： G01D18/00 ,  G01J1/58 ,  G01N21/00 ,  C12M1/34

    CPC分类号： G01N21/278 ,  B01L3/50857 ,  B01L3/5088 ,  B01L2200/148 ,  B01L2300/0822 ,  B01L2300/0829 ,  G01K11/14 ,  G01N1/28 ,  G01N21/6452 ,  G01N2001/2893 ,  G01N2015/1018 ,  G01T1/362 ,  Y10T436/10

    摘要： The invention concerns fluorescence standards, and in particular fluorescence standards for calibrating optical detectors. According to the invention, a fluorescent mineral or mixtures of minerals are employed for use as a fluorescence standard. The fluorescent mineral can be a naturally occurring mineral or a synthetically produced mineral. Preferred fluorescent minerals for use as fluorescence standards are corundum, fluorite, turquoise, amber, zircon, zoisite, iolite or cordierite, spinel, topaz, calcium fluorite, sphalerite or zincblende, calcite or calcspar, apatite, scheelite or calcium tungstate, willemite, feldspars, sodalite, a uranium mineral, a mineral containing Al3+, and in particular ruby and sapphire.

    摘要翻译： 本发明涉及荧光标准，特别是用于校准光学检测器的荧光标准。 根据本发明，荧光矿物或矿物质的混合物用作荧光标准。 荧光矿物可以是天然存在的矿物质或合成产生的矿物质。 用作荧光标准的优选的荧光矿物是刚玉，萤石，绿松石，琥珀，锆石，堇青石，石英或堇青石，尖晶石，黄玉，萤石，闪锌矿或闪锌矿，方解石或钙质，磷灰石，白钨矿或钨酸钙，硅锌矿，长石 ，钠盐，铀矿物质，含有Al3 +的矿物，特别是红宝石和蓝宝石。

    METHODS AND SYSTEMS FOR DETECTING NUCLEIC ACIDS
    4.
    发明申请
    METHODS AND SYSTEMS FOR DETECTING NUCLEIC ACIDS 审中-公开
    检测核酸的方法和系统

    公开(公告)号：US20080241838A1

    公开(公告)日：2008-10-02

    申请号：US11965837

    申请日：2007-12-28

    申请人： Kristian Scaboo ,  Vissarion Aivazachvili ,  Timothy Liu ,  Robert Eason ,  Konrad Faulstich

    发明人： Kristian Scaboo ,  Vissarion Aivazachvili ,  Timothy Liu ,  Robert Eason ,  Konrad Faulstich

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6834 ,  C12Q1/6823 ,  C12Q1/6825 ,  C12Q2531/113 ,  C12Q2521/307 ,  C12Q2525/204 ,  C12Q2565/607 ,  C12Q2561/109 ,  C12Q2565/519

    摘要： Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.

    摘要翻译： 描述了用于检测样品中靶核酸的方法和试剂盒。 在一些实施方案中，待分析的样品包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测的第二区 标记，扩增杂交引物的聚合酶和包含核酸酶活性的酶，其可以切割杂交的杂交探针，从而释放标记的探针片段。 在一些实施方案中，然后可以将样品与包含可以与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。 这些捕获探针比完整的杂交探针更容易与探针片段结合。 然后可以在支撑表面上检测标签。 以这种方式，可以实现探针片段与完整杂交探针之间的改善的区别。

    Compositions, methods, and kits for selective amplification of nucleic acids
    5.
    发明申请
    Compositions, methods, and kits for selective amplification of nucleic acids 审中-公开
    用于选择性扩增核酸的组合物，方法和试剂盒

    公开(公告)号：US20070212695A1

    公开(公告)日：2007-09-13

    申请号：US11331599

    申请日：2006-01-12

    申请人： Vissarion Aivazachvili ,  Konrad Faulstich ,  Tony Tran

    发明人： Vissarion Aivazachvili ,  Konrad Faulstich ,  Tony Tran

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/6853 ,  Y02A50/54 ,  C12Q2525/307 ,  C12Q2531/125 ,  C12Q2525/117 ,  C12Q2561/125 ,  C12Q2537/143

    摘要： The current teachings are directed to compositions, methods, and kits for selectively amplifying and for detecting target sequences. In some embodiments, a circularizable probe and/or a probe pair are disclosed for selectively amplifying target sequences. Methods for selectively amplifying target sequences are also disclosed, as are methods for detecting selectively amplified target sequences. Certain embodiments of the disclosed methods comprise a circularizable probe, a probe pair, comprising a first probe and a second probe, or both. In certain embodiments, a multiplicity of different circularizable probes, a multiplicity of different probe sets, or a multiplicity of different circularizable probes and a multiplicity of different probe sets are provided to selectively amplify or to detect a multiplicity of different target sequences, typically in a multiplex reaction. According to certain disclosed methods, surrogates of the target sequences are selectively amplified, including without limitation ligated probes, first amplification products, second amplification products, or combinations thereof. In some embodiments, selectively amplified target sequences or their surrogates are detected, directly or indirectly, indicating the presence of the corresponding target sequence. Kits to facilitate the performance of the disclosed methods are also provided.

    摘要翻译： 目前的教导涉及用于选择性扩增和检测靶序列的组合物，方法和试剂盒。 在一些实施方案中，公开了可圆化探针和/或探针对，用于选择性扩增靶序列。 还公开了选择性扩增靶序列的方法，以及用于检测选择性扩增的靶序列的方法。 所公开方法的某些实施方案包括可圆化的探针，包括第一探针和第二探针的探针对，或两者。 在某些实施方案中，提供多个不同的可圆化探针，多个不同的探针组或多个不同的可环化探针和多个不同的探针组，以选择性地扩增或检测多种不同靶序列，通常在 多重反应。 根据某些公开的方法，选择性扩增靶序列的替代物，包括但不限于连接的探针，第一扩增产物，第二扩增产物或其组合。 在一些实施方案中，直接或间接检测选择性扩增的靶序列或其替代物，表明存在相应的靶序列。 还提供了用于促进所公开方法的性能的套件。

    Methods and Systems for Detecting Nucleic Acids
    8.
    发明申请
    Methods and Systems for Detecting Nucleic Acids 审中-公开
    检测核酸的方法和系统

    公开(公告)号：US20110014617A1

    公开(公告)日：2011-01-20

    申请号：US12816006

    申请日：2010-06-15

    申请人： Kristian Scaboo ,  Vissarion Aivazachvili ,  Timothy Liu ,  Robert Eason ,  Konrad Faulstich

    发明人： Kristian Scaboo ,  Vissarion Aivazachvili ,  Timothy Liu ,  Robert Eason ,  Konrad Faulstich

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6834 ,  C12Q1/6823 ,  C12Q1/6825 ,  C12Q2531/113 ,  C12Q2521/307 ,  C12Q2525/204 ,  C12Q2565/607 ,  C12Q2561/109 ,  C12Q2565/519

    摘要： Methods and kits for detecting a target nucleic acid in a sample are described. In some embodiments, the sample to be analyzed includes a primer which hybridizes to at least a portion of the target nucleic acid, a probe having a first region which hybridizes to at least a portion of the target nucleic acid and a second region having a detectable label, a polymerase which extends the hybridized primer and an enzyme comprising nuclease activity that can cleave the hybridized hybridization probe to thereby release a labeled probe fragment. In some embodiments, the sample can then be contacted with a solid support comprising surface bound capture probes which can hybridize to the labeled probe fragment(s). These capture probes more readily bind to the probe fragment(s) than to the intact hybridization probe. The label can then be detected on the support surface. In this manner, improved discrimination between the probe fragments and the intact hybridization probes can be achieved.

    摘要翻译： 描述了用于检测样品中靶核酸的方法和试剂盒。 在一些实施方案中，待分析的样品包括与靶核酸的至少一部分杂交的引物，具有与靶核酸的至少一部分杂交的第一区的探针和具有可检测的第二区 标记，扩增杂交引物的聚合酶和包含核酸酶活性的酶，其可以切割杂交的杂交探针，从而释放标记的探针片段。 在一些实施方案中，然后可以将样品与包含可以与标记的探针片段杂交的表面结合捕获探针的固体支持物接触。 这些捕获探针比完整的杂交探针更容易与探针片段结合。 然后可以在支撑表面上检测标签。 以这种方式，可以实现探针片段与完整杂交探针之间的改善的区别。

    5′-modified nucleotides and the application thereof in molecular biology and medicine
    9.
    发明授权
    5′-modified nucleotides and the application thereof in molecular biology and medicine 失效
    5'-修饰的核苷酸及其在分子生物学和医学中的应用

    公开(公告)号：US06627416B1

    公开(公告)日：2003-09-30

    申请号：US09646890

    申请日：2001-01-10

    申请人： Konrad Faulstich ,  Wilhelm Ansorge

    发明人： Konrad Faulstich ,  Wilhelm Ansorge

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6869 ,  C12Q2527/119 ,  C12Q2527/101 ,  C12Q2525/113

    摘要： The invention relates to 5′-modified nucleotides and to nucleic acids which contain these nucleotides. Processes for incorporating the 5′-modified nucleotides into nucleic acids, and the subsequent site-specific cleavage of the nucleic acids at the 5′-modified monomer building blocks, are also disclosed. These processes can be employed for nucleic acid sequencing, for generating nucleic acid libraries, for detecting mutations, for preparing support-bound nucleic acids and for pharmaceutical purposes.

    摘要翻译： 本发明涉及5'-修饰的核苷酸和含有这些核苷酸的核酸。 还公开了将5'-修饰的核苷酸并入核酸的方法，以及随后的5'-修饰的单体结构单元上的核酸的位点特异性切割。 这些方法可用于核酸测序，产生核酸文库，检测突变，制备载体结合核酸和用于制药目的。