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公开(公告)号:US20050194316A1
公开(公告)日:2005-09-08
申请号:US11121541
申请日:2005-05-03
申请人: Farzad Pourahmadi , William McMillan , Jesus Ching , Ronald Chang , Lee Christel , Gregory Kovacs , M. Northrup , Kurt Petersen
发明人: Farzad Pourahmadi , William McMillan , Jesus Ching , Ronald Chang , Lee Christel , Gregory Kovacs , M. Northrup , Kurt Petersen
IPC分类号: G01N31/20 , B01D11/00 , B01J19/00 , B01L3/00 , B01L7/00 , C12M1/00 , C12M1/26 , C12M1/33 , C12M1/34 , C40B60/14 , G01N1/28 , G01N30/60 , G01N33/50 , G01N35/08 , G01N37/00
CPC分类号: G01N30/6095 , B01J2219/00432 , B01J2219/00529 , B01L3/502715 , B01L3/502753 , B01L7/52 , B01L2200/0621 , B01L2200/0631 , B01L2200/0636 , B01L2200/10 , B01L2300/0816 , B01L2300/0883 , B01L2300/1827 , B01L2400/0421 , B01L2400/046 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , B01L2400/086 , C12M47/02 , C12M47/06 , C40B60/14 , G01N30/6091 , Y10T436/25375 , Y10T436/255
摘要: An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.
摘要翻译: 将样品通过将样品引入具有样品端口和从样品端口延伸的第一流路的盒中而将分析物与流体样品分离。 第一流路包括提取室,其包含用于从样品捕获分析物的固体支持物。 所述筒具有用于从所述提取室洗脱所捕获的分析物的第二流动路径,所述第二流在通过所述提取室之后从所述第一流动路径发散。 样品被迫流过提取室并进入废物室,从而当样品流过提取室时用固体支持物捕获分析物。 然后通过迫使洗脱液体流过提取室并沿着第二流动路径,将捕获的分析物从萃取室洗脱。
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公开(公告)号:US20060014200A1
公开(公告)日:2006-01-19
申请号:US11225247
申请日:2005-09-12
申请人: William McMillan , Lee Christel , David Borkholder , Steven Young
发明人: William McMillan , Lee Christel , David Borkholder , Steven Young
CPC分类号: C12Q1/6851 , B01L7/52 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6471 , G01N2035/00366 , G01N2035/00376 , G01N2035/0097 , G01N2201/062 , G01N2201/0627 , C12Q2537/165 , C12Q2545/107 , C12Q2545/113 , C12Q2545/101
摘要: An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. A controller in communication with the detection mechanism is programmed to store signal values defining a growth curve for the nucleic acid sequence, determine a derivative of the growth curve, and calculate a cycle number or time value associated with a characteristic of the derivative.
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公开(公告)号:US20050255516A1
公开(公告)日:2005-11-17
申请号:US11170620
申请日:2005-06-28
申请人: William McMillan , Lee Christel , David Borkholder , Steven Young
发明人: William McMillan , Lee Christel , David Borkholder , Steven Young
IPC分类号: B01L7/00 , C07H21/02 , C07H21/04 , C12M1/00 , C12M1/34 , C12N9/98 , C12P19/34 , C12Q1/00 , C12Q1/68 , G01N21/27 , G01N21/64 , G01N33/48 , G01N33/50 , G01N35/00 , G06F19/00
CPC分类号: C12Q1/6851 , B01L7/52 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/645 , G01N2021/6417 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6471 , G01N2035/00366 , G01N2035/00376 , G01N2035/0097 , G01N2201/062 , G01N2201/0627 , C12Q2537/165 , C12Q2545/107 , C12Q2545/113 , C12Q2545/101
摘要: A method for determining an unknown starting quantity of a target nucleic acid sequence in a test sample comprises the steps of amplifying the unknown starting quantity of the target nucleic acid sequence in the test sample and known starting quantities of a calibration nucleic acid sequence in respective calibration samples; and determining a respective threshold value for each of the nucleic acid sequences using a derivative of a growth curve derived for the sequence. The starting quantity of the target nucleic acid sequence in the test sample is determined using the threshold value determined for the target sequence and a calibration curve derived from the threshold values determined for the known starting quantities of the calibration nucleic acid sequences. The invention also provides methods for determining a starting quantity of a nucleic acid sequence in a sample using quantitative internal controls or using internal standards.
摘要翻译: 用于确定测试样品中目标核酸序列的未知起始量的方法包括以下步骤:扩增测试样品中目标核酸序列的未知起始量和已校准核酸序列的开始量在相应的校准 样品; 以及使用由该序列导出的生长曲线的衍生物来确定每个核酸序列的相应阈值。 使用针对靶序列确定的阈值和从针对校准核酸序列的已知起始量确定的阈值得到的校准曲线来确定测试样品中靶核酸序列的起始量。 本发明还提供了使用定量内部对照或使用内部标准确定样品中核酸序列的起始量的方法。
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