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公开(公告)号：US08338093B2
公开(公告)日：2012-12-25
申请号：US11323067
申请日：2005-12-30
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00452 , B01J2219/00454 , B01J2219/00497 , B01J2219/00527 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00626 , B01J2219/00659 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C40B80/00
摘要： Methods are presented for generating large sets for polymers. The methods employ high density oligonucleotide array.
摘要翻译： 提出了用于生成大量聚合物的方法。 该方法采用高密度寡核苷酸阵列。
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2.发明申请Methods and Compositions for Selective Labeling of Different Biotinylated Targets within Multicolor or Multilabel Assays 有权用于多色或多标记测定中不同生物素化靶标的选择性标记的方法和组合公开(公告)号：US20120226027A1
公开(公告)日：2012-09-06
申请号：US13329175
申请日：2011-12-16
IPC分类号： C07K1/13
CPC分类号： C07K1/13 , G01N33/582 , Y10T436/10 , Y10T436/105831 , Y10T436/107497 , Y10T436/25125 , Y10T436/25375
摘要： Disclosed are compositions and methods for the labeling of two or more targets with different labels. Specifically, disclosed are compositions for biotin and the protection of biotin within multilabel assays which employ the biotin-biotin binding protein binding relationship for each distinct label in relation to targets such as nucleic acids, polypeptides, antibodies or cells. These multilabel assays are enabled through the use of biotin with desthiobiotin, orthogonal protecting schemes for biotin, or a combination of the approaches.
摘要翻译： 公开了用于标记具有不同标签的两个或更多个靶标的组合物和方法。 具体地，公开了生物素的组合物和在多标记测定中保护生物素，其与针对诸如核酸，多肽，抗体或细胞的靶相对应的每个不同标记使用生物素 - 生物素结合蛋白结合关系。 通过使用具有脱硫生物素的生物素，生物素的正交保护方案或这些方法的组合来实现这些多标记测定。
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公开(公告)号：US20120094872A1
公开(公告)日：2012-04-19
申请号：US13315078
申请日：2011-12-08
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00434 , B01J2219/00439 , B01J2219/00497 , B01J2219/00576 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00675 , B01J2219/00677 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C08G77/16 , C08G77/26 , C09D183/06 , C09D183/08 , C40B50/18 , C08L83/00
摘要： Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.
摘要翻译： 含有两种或更多种硅烷化试剂的混合物的硅烷化组合物，其中至少一种硅烷化试剂包括能够支持聚合物合成的官能团和至少一种硅烷化试剂，不包括能够支持聚合物合成的官能团，可用于调节活性部位密度 和表面的水解稳定性。 这些组合物在制备聚合物阵列之前特别可用于将表面硅烷化，并提供增加的杂交结果。
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公开(公告)号：US09556360B2
公开(公告)日：2017-01-31
申请号：US13315078
申请日：2011-12-08
IPC分类号： C12M1/34 , G01N33/552 , G01N33/554 , C12Q1/00 , C09D183/08 , B01J19/00 , B82Y30/00 , C08G77/16 , C08G77/26 , C09D183/06 , C40B50/18
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00434 , B01J2219/00439 , B01J2219/00497 , B01J2219/00576 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00675 , B01J2219/00677 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C08G77/16 , C08G77/26 , C09D183/06 , C09D183/08 , C40B50/18 , C08L83/00
摘要： Silanation compositions containing a mixture of two or more silanation reagents, where at least one silanation reagent includes a functional group capable of supporting polymer synthesis and at least one silanation reagent includes no functional group capable of supporting polymer synthesis are useful in modulating the active site density and hydrolytic stability of a surface. These compositions are particularly useful in silanating a surface prior to preparation of a polymer array and provide for increased hybridization results.
摘要翻译： 含有两种或更多种硅烷化试剂的混合物的硅烷化组合物，其中至少一种硅烷化试剂包括能够支持聚合物合成的官能团和至少一种硅烷化试剂，不包括能够支持聚合物合成的官能团，可用于调节活性部位密度 和表面的水解稳定性。 这些组合物在制备聚合物阵列之前特别可用于将表面硅烷化，并提供增加的杂交结果。
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5.发明授权Use of acid scavengers for the synthesis of standard length and long-mer nucleic acid arrays 有权使用酸清除剂合成标准长度和长链核酸阵列公开(公告)号：US08062844B2
公开(公告)日：2011-11-22
申请号：US13011030
申请日：2011-01-21
CPC分类号： B01J19/0046 , B01J2219/00317 , B01J2219/00432 , B01J2219/00439 , B01J2219/00441 , B01J2219/00448 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00646 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00693 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C07H21/00 , C07K1/047 , C40B50/14 , Y02P20/55
摘要： Protective groups which may be cleaved with an activatable deprotecting reagents are employed to achieve a highly sensitive, high resolution, combinatorial synthesis of pattern arrays of diverse polymers. In preferred embodiments of the instant invention, the activatable deprotecting reagent is a photoacid generator and the protective groups are DMT for nucleic acids and tBOC for amino acids. This invention has a wide variety of applications and is particularly useful for the solid phase combinatorial synthesis of polymers.
摘要翻译： 可以用可活化的去保护试剂切割的保护基用于实现多种聚合物的图案阵列的高灵敏度，高分辨率，组合合成。 在本发明的优选实施方案中，可活化的去保护试剂是光酸产生剂，保护基是核酸的DMT和氨基酸的tBOC。 本发明具有广泛的应用，并且对聚合物的固相组合合成特别有用。
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公开(公告)号：US20110269631A1
公开(公告)日：2011-11-03
申请号：US12899540
申请日：2010-10-06
申请人： Glenn K. Fu , Glenn H. McGall , Robert G. Kuimelis , Jing Hu , Pei-Hua Wang
发明人： Glenn K. Fu , Glenn H. McGall , Robert G. Kuimelis , Jing Hu , Pei-Hua Wang
CPC分类号： C12Q1/6837 , C12Q1/6844 , C12Q1/6858 , C12Q1/6874 , C12Q1/6876 , C12Q2600/106 , C12Q2600/154 , C12Q2600/156 , C40B50/06 , C12Q2531/125 , C12Q2565/513 , C12Q2537/143 , C12Q2531/113 , C12Q2521/307
摘要： Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number.
摘要翻译： 提供了用于选择靶的多重放大和扩增靶的分析的方法。 在优选的方面，扩增和分析发生在相同的固体支持物上，优选在局部区域例如珠粒或阵列特征中。 通过与探针杂交，然后连接靶的末端以形成闭合圆，靶标被循环。 然后将目标用作扩展阵列绑定探针的模板，导致具有多个目标拷贝的扩展探针。 然后可以分析扩展的探针。 该方法可用于拷贝数的基因分型，测序和分析。
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公开(公告)号：US20110046343A1
公开(公告)日：2011-02-24
申请号：US12938231
申请日：2010-11-02
CPC分类号： B01J19/0046 , B01J2219/00452 , B01J2219/00454 , B01J2219/00497 , B01J2219/00527 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00626 , B01J2219/00659 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B82Y30/00 , C40B80/00
摘要： Methods are presented for generating large sets for polymers. The methods employ high density oligonucleotide array.
摘要翻译： 提出了用于生成大量聚合物的方法。 该方法采用高密度寡核苷酸阵列。
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公开(公告)号：US07803934B2
公开(公告)日：2010-09-28
申请号：US12360945
申请日：2009-01-28
CPC分类号： B01J19/0046 , B01J2219/00427 , B01J2219/00527 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C40B40/06 , C40B50/14 , C40B80/00
摘要： The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from about 101 to about 105 different sequences, generally between 25-160 bases in length).
摘要翻译： 本发明提供大量平行的寡核苷酸合成和纯化，用于利用大量定义的高保真寡核苷酸（例如约101至约105个不同序列，通常在25-160个碱基之间）的应用。
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公开(公告)号：US07547775B2
公开(公告)日：2009-06-16
申请号：US11324834
申请日：2006-01-03
CPC分类号： B01J19/0046 , B01J2219/00427 , B01J2219/00527 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C40B40/06 , C40B50/14 , C40B80/00
摘要： The present invention provides massively parallel oligonucleotide synthesis and purification for applications that utilize large collections of defined high-fidelity oligonucleotides (e.g., from about 101 to about 105 different sequences, generally between 25-160 bases in length).
摘要翻译： 本发明提供大量平行的寡核苷酸合成和纯化，用于利用大量定义的高保真寡核苷酸（例如约101至约105个不同序列，通常在25-160个碱基之间）的应用。
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10.发明申请Oxide Layers on Silicone Substrates for Effective Confocal Laser Microscopy 审中-公开氧化层在有机硅基底上有效共焦激光显微镜公开(公告)号：US20120264644A1
公开(公告)日：2012-10-18
申请号：US13533594
申请日：2012-06-26
申请人： Robert G. Kuimelis , Zihui Chen , Glenn H. McGall
发明人： Robert G. Kuimelis , Zihui Chen , Glenn H. McGall
IPC分类号： C40B30/04
CPC分类号： G01N21/6452 , G01N21/6428 , G01N21/6458 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , Y10T436/25
摘要： Methods of performing confocal laser microscopy on a polymer array disposed on a silicon wafer substrate, the method comprising the steps of providing a silicon wafer substrate having a top side and a bottom side, coating the top side of the silicon wafer with an oxide coating to provide an oxide coated wafer, covalently coupling a plurality of probes to the top side of the coated wafer to provide a fixed polymer array, hybridizing the fixed polymer array with a plurality of labeled ligands, and assaying for one or more hybridized ligands using confocal laser fluorescence microscopy to detect hybridization are provided.
摘要翻译： 在设置在硅晶片基板上的聚合物阵列上进行共焦激光显微镜的方法，所述方法包括以下步骤：提供具有顶侧和底侧的硅晶片衬底，用氧化物涂层涂覆硅晶片的顶侧 提供氧化物涂覆的晶片，将多个探针共价耦合到涂覆的晶片的顶侧，以提供固定的聚合物阵列，将固定的聚合物阵列与多个标记的配体杂交，以及使用共焦激光测定一个或多个杂交配体 提供荧光显微镜检测杂交。
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