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    Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation
    7.
    发明授权
    Labeled peptides, proteins and antibodies and processes and intermediates useful for their preparation 失效
    标记的肽，蛋白质和抗体以及用于其制备的方法和中间体

    公开(公告)号：US07176037B2

    公开(公告)日：2007-02-13

    申请号：US10455713

    申请日：2003-06-03

    申请人： Klaus M. Hahn ,  Alexei Toutchkine ,  Rajeev Muthyala ,  Vadim Kraynov ,  Steven J. Bark ,  Dennis R. Burton ,  Chester Chamberlain

    发明人： Klaus M. Hahn ,  Alexei Toutchkine ,  Rajeev Muthyala ,  Vadim Kraynov ,  Steven J. Bark ,  Dennis R. Burton ,  Chester Chamberlain

    IPC分类号： G01N33/533 ,  G01N33/532 ,  G01N21/76 ,  G01N33/53 ,  C07K1/13 ,  C07D277/62

    CPC分类号： C07K1/13 ,  C07K1/1077

    摘要： The invention provides peptide synthons having protected functional groups for attachment of desired moieties (e.g. functional molecules or probes). Also provided are peptide conjugates prepared from such synthons, and synthon and conjugate preparation methods including procedures for identifying optimum probe attachment sites. Biosensors are provided having functional molecules that can locate and bind to specific biomolecules within living cells. Biosensors can detect chemical and physiological changes in those biomolecules as living cells are moving, metabolizing and reacting to its environment. Methods are included for detecting GTP activation of a Rho GTPase protein using polypeptide biosensors. When the biosensor binds GTP-activated Rho GTPase protein, an environmentally sensitive dye emits a signal of a different lifetime, intensity or wavelength than when not bound. New fluorophores whose fluorescence responds to environmental changes are also provided that have improved detection and attachment properties, and that can be used in living cells, or in vitro.

    摘要翻译： 本发明提供具有用于连接所需部分（例如功能分子或探针）的保护的官能团的肽合成子。 还提供了由这种合成子制备的肽缀合物，以及合成子和缀合物制备方法，包括鉴定最佳探针附着位点的程序。 生物传感器具有能够定位并结合活细胞内的特定生物分子的功能分子。 生物传感器可以检测生物分子中的化学和生理变化，因为活细胞正在移动，代谢并对其环境作出反应。 包括使用多肽生物传感器检测Rho GTPase蛋白的GTP活化的方法。 当生物传感器结合GTP激活的Rho GTPase蛋白质时，环境敏感的染料会发出与未结合不同的寿命，强度或波长的信号。 还提供了荧光对环境变化的响应的新荧光团，其具有改进的检测和附着性质，并且可用于活细胞或体外。

    METHODS AND COMPOSITIONS FOR INDENTIFYING BINDING PARTNERS FROM LIBRARIES OF BIOMOLECULES
    10.
    发明申请
    METHODS AND COMPOSITIONS FOR INDENTIFYING BINDING PARTNERS FROM LIBRARIES OF BIOMOLECULES 审中-公开
    用于从生物分子图书馆中鉴别合伙人的方法和组合

    公开(公告)号：US20100216659A1

    公开(公告)日：2010-08-26

    申请号：US12452969

    申请日：2008-06-16

    申请人： Diana R. Bowley ,  Teresa Jones ,  Dennis R. Burton ,  Richard A. Lerner

    发明人： Diana R. Bowley ,  Teresa Jones ,  Dennis R. Burton ,  Richard A. Lerner

    IPC分类号： C40B30/04 ,  C40B40/02

    CPC分类号： C12N15/1037 ,  B01J2219/00725 ,  B01J2219/00743 ,  C40B40/02

    摘要： The present invention provides methods for identifying cognate binding pairs from two libraries of biomolecules (e.g., polypeptides). The methods typically involve displaying a first library of candidate biomolecules (e.g., receptors or epitopes) on a first replicable genetic package (e.g., a cell surface display platform) and displaying a second library of candidate biomolecules (e.g., ligands) on a second replicable genetic package (e.g., a phage display platform), contacting the first library with the second library, and then selecting members of the first library to which a member of the second library is bound. Also provided in the invention are compositions and kits for carrying out the methods of the invention.

    摘要翻译： 本发明提供了从两个生物分子文库（例如，多肽）鉴定同源结合对的方法。 所述方法通常涉及在第一可复制遗传包（例如，细胞表面展示平台）上显示候选生物分子（例如，受体或表位）的第一文库，并在第二可复制物上显示候选生物分子（例如，配体）的第二文库 遗传包（例如噬菌体展示平台），将第一个库与第二个库联系起来，然后选择第二个库的成员绑定到的第一个库的成员。 本发明还提供了用于实施本发明方法的组合物和试剂盒。