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21 条记录 (耗时 0.029 秒)

    Glycoprotein and process for producing the same
    5.
    发明授权
    Glycoprotein and process for producing the same 失效
    糖蛋白及其制备方法

    公开(公告)号：US07579166B2

    公开(公告)日：2009-08-25

    申请号：US10480790

    申请日：2002-06-14

    申请人： Yasunori Chiba ,  Yoshifumi Jigami ,  Hitoshi Sakuraba ,  Kazuo Kobayashi ,  Makoto Takeuchi ,  Yoriko Takeuchi, legal representative

    发明人： Yasunori Chiba ,  Yoshifumi Jigami ,  Hitoshi Sakuraba ,  Kazuo Kobayashi ,  Makoto Takeuchi

    IPC分类号： C12N15/00 ,  C12P21/00 ,  C12Q1/68 ,  C07K14/00

    CPC分类号： C12P21/005 ,  A61K36/064 ,  A61K38/17 ,  C12N9/1051 ,  A61K2300/00

    摘要： A process for producing a lysosomal enzyme having a mannose-6-phosphate-containing acidic sugar chain, wherein the process comprising: culturing in a medium yeast cells obtained by introducing a lysosomal enzyme gene into a sugar chain biosynthetic enzyme gene mutant strain of yeast, collecting a lysosomal enzyme having a phosphate-containing sugar chain from the culture, and then treating the enzyme with α-mannosidase; and pharmaceutical compositions for treatment of human lysosomal enzyme deficiencies produced by the process. The genetic engineering technique using the yeast according to the present invention allows large-amount and high-purity production of a glycoprotein having a phosphate-containing acidic sugar chain which can serve as a labeling marker for transporting into lysosomes in cells of mammals such as human. The glycoprotein having a phosphate-containing acidic sugar chain according to the invention may be utilized as a drug effective in treatment of human lysosomal enzyme deficiencies, etc.

    摘要翻译： 一种生产含有甘露糖-6-磷酸的酸性糖链的溶酶体酶的方法，其中所述方法包括：在通过将溶酶体酶基因导入酵母的糖链生物合成酶基因突变菌株获得的培养基中培养酵母， 从培养物中收集含有含磷酸盐糖链的溶酶体酶，然后用α-甘露糖苷酶处理酶; 以及用于治疗由该方法产生的人溶酶体酶缺陷的药物组合物。 使用根据本发明的酵母的基因工程技术允许大量和高纯度产生具有含磷酸盐的酸性糖链的糖蛋白，其可以用作在诸如人类的哺乳动物的细胞中输送到溶酶体的标记标记 。 具有根据本发明的含磷酸盐酸性糖链的糖蛋白可以用作治疗人溶酶体酶缺陷等的药物。

    Glycoprotein and process for producing the same
    6.
    发明申请
    Glycoprotein and process for producing the same 失效
    糖蛋白及其制备方法

    公开(公告)号：US20050064539A1

    公开(公告)日：2005-03-24

    申请号：US10480790

    申请日：2002-06-14

    申请人： Yasunori Chiba ,  Yoshifumi Jigami ,  Hitoshi Sakuraba ,  Kazuo Kobayashi ,  Makoto Takeuchi ,  Yoriko Takeuchi

    发明人： Yasunori Chiba ,  Yoshifumi Jigami ,  Hitoshi Sakuraba ,  Kazuo Kobayashi ,  Makoto Takeuchi ,  Yoriko Takeuchi

    IPC分类号： C12N15/09 ,  A61K36/064 ,  A61K38/00 ,  A61K38/17 ,  A61P9/00 ,  A61P9/14 ,  A61P13/12 ,  A61P17/00 ,  A61P19/00 ,  A61P27/02 ,  A61P43/00 ,  C08B37/00 ,  C12N9/10 ,  C12N9/40 ,  C12P21/00 ,  C12P21/06 ,  C12N1/18

    CPC分类号： C12P21/005 ,  A61K36/064 ,  A61K38/17 ,  C12N9/1051 ,  A61K2300/00

    摘要： A process for producing a lysosomal enzyme having a mannose-6-phosphate-containing acidic sugar chain, wherein the process comprising: culturing in a medium yeast cells obtained by introducing a lysosomal enzyme gene into a sugar chain biosynthetic enzyme gene mutant strain of yeast, collecting a lysosomal enzyme having a phosphate-containing sugar chain from the culture, and then treating the enzyme with α-mannosidase; and pharmaceutical compositions for treatment of human lysosomal enzyme deficiencies produced by the process. The genetic engineering technique using the yeast according to the present invention allows large-amount and high-purity production of a glycoprotein having a phosphate-containing acidic sugar chain which can serve as a labeling marker for transporting into lysosomes in cells of mammals such as human. The glycoprotein having a phosphate-containing acidic sugar chain according to the invention may be utilized as a drug effective in treatment of human lysosomal enzyme deficiencies, etc.

    摘要翻译： 一种生产含有甘露糖-6-磷酸的酸性糖链的溶酶体酶的方法，其中所述方法包括：在通过将溶酶体酶基因导入酵母的糖链生物合成酶基因突变菌株获得的培养基中培养酵母， 从培养物中收集含有含磷酸盐糖链的溶酶体酶，然后用α-甘露糖苷酶处理酶; 以及用于治疗由该方法产生的人溶酶体酶缺陷的药物组合物。 使用根据本发明的酵母的基因工程技术允许大量和高纯度产生具有含磷酸盐的酸性糖链的糖蛋白，其可以用作在诸如人类的哺乳动物的细胞中输送到溶酶体的标记标记 。 具有根据本发明的含磷酸盐酸性糖链的糖蛋白可以用作治疗人溶酶体酶缺陷等的药物。

    Methylotrophic yeast producing mammalian type sugar chain
    7.
    发明授权
    Methylotrophic yeast producing mammalian type sugar chain 有权
    甲基营养酵母产生哺乳动物型糖链

    公开(公告)号：US07972809B2

    公开(公告)日：2011-07-05

    申请号：US10511436

    申请日：2003-04-28

    申请人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasunori Chiba

    发明人： Kazuo Kobayashi ,  Yoshinori Kitagawa ,  Toshihiro Komeda ,  Nagako Kawashima ,  Yoshifumi Jigami ,  Yasunori Chiba

    IPC分类号： C12P21/00 ,  C12N15/01

    CPC分类号： C12P19/26 ,  C07K16/243 ,  C07K2317/21 ,  C12N1/14 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1048 ,  C12N9/1051 ,  C12N9/2488 ,  C12N9/60 ,  C12N9/88 ,  C12N9/93 ,  C12P21/02 ,  C12Y101/01085 ,  C12Y101/03013 ,  C12Y102/01012 ,  C12Y302/01024 ,  C12Y402/01019 ,  C12Y603/02006

    摘要： This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an α-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the α-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity. By introducing a mammalian type sugar chain biosynthesizing gene into the above-described mutant, a mammalian type sugar chain, such as a hybrid or complex, or a protein comprising a mammalian type sugar chain can be efficiently produced.

    摘要翻译： 本发明提供一种生产包含哺乳动物型糖链的糖蛋白的方法，其特征在于该方法包括将α-1,2-甘露糖苷酶基因导入具有糖链生物合成酶基因突变的甲基营养酵母中，因此 α-1,2-甘露糖苷酶基因在酵母中有效启动子的控制下表达; 在介质中培养具有转移到其中的异源基因的甲基营养酵母细胞; 从培养物中获得包含哺乳动物型糖链的糖蛋白。 使用新制造的具有糖链突变的甲基营养酵母，可以大量生产与由哺乳动物细胞如人细胞产生的高甘露糖型糖链或包含这种中性糖链的糖蛋白相同的中性糖链 纯度高。 通过将哺乳动物型糖链生物合成基因导入到上述突变体中，可以有效地制备哺乳动物型糖链，例如杂合体或复合物，或包含哺乳动物型糖链的蛋白质。