公开(公告)号：US06858186B2

公开(公告)日：2005-02-22

申请号：US10035907

申请日：2001-12-24

申请人： Richard O. Hilson ,  Bill J. Peck ,  Eric M. Leproust

发明人： Richard O. Hilson ,  Bill J. Peck ,  Eric M. Leproust

IPC分类号： A61B17/06 ,  A61B19/02 ,  B01J19/00 ,  B01L9/00 ,  B05D3/00 ,  C12M1/34 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B50/14 ,  C40B60/14

CPC分类号： C40B50/14 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00328 ,  B01J2219/00353 ,  B01J2219/00369 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00518 ,  B01J2219/00527 ,  B01J2219/00536 ,  B01J2219/00538 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00664 ,  B01J2219/00675 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B60/14

摘要： Apparatus and methods are disclosed for storing a plurality of supports having a plurality of chemical compounds bound to the surfaces of the supports. In the apparatus, a mechanism for diffusively introducing pressurized gas into the apparatus is in fluid communication with an outlet element comprising a plurality of openings. A holding chamber for the supports is in fluid communication with the outlet element. The outlet element and the holding chamber are disposed such that gas flow through the chamber is substantially uniform and unidirectional. The holding chamber comprises an opening sufficient to permit movement of the supports to and from the holding chamber and comprises a plurality of holding elements for holding the supports.

摘要翻译： 公开了用于存储多个支撑体的装置和方法，所述多个支撑件具有结合到支撑体表面的多个化学化合物。 在该装置中，用于将加压气体扩散地引入到装置中的机构与包括多个开口的出口元件流体连通。 用于支撑件的保持室与出口元件流体连通。 出口元件和保持室被布置成使得通过腔室的气体流体基本上均匀且单向。 保持室包括足以允许支撑件移动到保持室和从保持室移动的开口，并且包括用于保持支撑件的多个保持元件。

公开(公告)号：US07220573B2

公开(公告)日：2007-05-22

申请号：US10177358

申请日：2002-06-21

申请人： Lawrence R. Shea ,  Douglas G. Summers ,  Richard O. Hilson

发明人： Lawrence R. Shea ,  Douglas G. Summers ,  Richard O. Hilson

IPC分类号： C07H21/04 ,  C12Q1/68

CPC分类号： B01L9/52 ,  B01J19/0046 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502 ,  B01L2300/043 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0877 ,  C40B40/06 ,  C40B40/10 ,  G01N33/54366

摘要： Array assay devices and methods for using the same in array based assays are provided. The subject devices are characterized by having a base dimensioned to hold a substrate having at least one array, a cover dimensioned to cover the substrate and a snap-fit for holding the cover and the base together to enclose a space between the base and the cover. The subject invention also includes methods for performing an array assay. In the subject methods, a subject device is provided and a substrate having at least one array is positioned on the base. The snap-fit is engaged to hold the cover and the base together to enclose a space between the base and the cover. Next, a sample is contacted with the at least one array and an array assay is performed. The subject invention also includes kits which include the subject devices.

摘要翻译： 提供了阵列测定装置及其在基于阵列的测定中的使用方法。 主题装置的特征在于具有尺寸适于保持具有至少一个阵列的基板的基座，尺寸适于覆盖基板的盖子和用于将盖子和基座保持在一起以将基部和盖子之间的空间封闭的卡扣配合 。 本发明还包括用于进行阵列测定的方法。 在本发明的方法中，提供了一种主体装置，并且具有至少一个阵列的基板位于基座上。 卡扣配合被接合以将盖和基座保持在一起以封闭基座和盖之间的空间。 接下来，使样品与至少一个阵列接触并进行阵列测定。 本发明还包括包括本发明装置的试剂盒。

公开(公告)号：US06881579B2

公开(公告)日：2005-04-19

申请号：US09919073

申请日：2001-07-30

申请人： Richard O. Hilson ,  Edward P. Donlon ,  Donald J. Schremp ,  Laurence R. Shea ,  Carol T. Schembri ,  Joseph P. Fredrick

发明人： Richard O. Hilson ,  Edward P. Donlon ,  Donald J. Schremp ,  Laurence R. Shea ,  Carol T. Schembri ,  Joseph P. Fredrick

IPC分类号： G01N35/00 ,  G01N35/04 ,  G01N35/02 ,  G01N33/50

CPC分类号： G01N35/04 ,  G01N35/00029 ,  G01N2035/00158 ,  G01N2035/0444 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114998 ,  Y10T436/142222 ,  Y10T436/143333

摘要： Apparatus and methods are disclosed for processing samples on the surface of supports that are contained in support housings. Biopolymer features are attached to the surfaces of the supports. An apparatus comprises an input element, a holding device for holding a plurality of support housings, one or more fluid dispensing stations, and an output element. Each of the support housings contains a support having attached thereto a plurality of biopolymer features. The holding device is movably mounted with respect to other components of the apparatus. The holding device is adapted to receive a support housing from the input element. The output element is adapted to receive a support housing from the holding device. The apparatus is adapted to index each support housing for a predetermined operation. In use, each of the support housings is moved to one or more processing stations by means of the movable holding device. The location and identity of each of the support housings is indexed. Fluid is applied to the surface of each of the supports at the processing stations to process the samples. Each of the support housings is moved away from the fluid dispensing stations, and fluid is physically removed from each of the supports within the support housings.

摘要翻译： 公开了用于在载体表面上包含在支撑壳体中的样品进行处理的装置和方法。 生物聚合物特征附着在支撑体的表面上。 一种装置包括输入元件，用于保持多个支撑壳体的保持装置，一个或多个流体分配站以及输出元件。 每个支撑壳体包含附着有多个生物聚合物特征的支撑体。 保持装置相对于装置的其它部件可移动地安装。 保持装置适于从输入元件接收支撑壳体。 输出元件适于从保持装置接纳支撑壳体。 该装置适于为每个支撑壳体索引预定的操作。 在使用中，通过可移动保持装置将每个支撑壳体移动到一个或多个处理站。 每个支撑壳体的位置和身份被索引。 将流体施加到处理站处的每个支撑件的表面以处理样品。 每个支撑壳体移动离开流体分配站，并且流体物理地从支撑壳体内的每个支撑件移除。

公开(公告)号：US07008788B2

公开(公告)日：2006-03-07

申请号：US09919062

申请日：2001-07-30

申请人： Donald J. Schremp ,  Richard O. Hilson ,  Edward P. Donlon

发明人： Donald J. Schremp ,  Richard O. Hilson ,  Edward P. Donlon

IPC分类号： C12M3/00

CPC分类号： B01L9/52 ,  B01F11/0014 ,  B01F11/0022 ,  B01F13/0059 ,  B01F13/02 ,  B01F13/0272 ,  B01F2005/0017 ,  B01F2215/0454 ,  B01F2215/0468 ,  B01L3/502 ,  B01L3/508 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/0851 ,  C12M23/04 ,  C12Q1/6834 ,  Y10S436/809 ,  Y10S436/823

摘要： Devices and methods are disclosed for containing and processing samples on the surface of supports. Biopolymer features are attached to the surfaces of the supports. A device in accordance with the invention comprises a housing and a support confined by the housing. The housing comprises a well having walls and at least one wall extending from the edge of the well. The height of the walls of the well is at least great enough, and the design of the at least one wall is such, that liquid contained in the well is not drawn out of the well to any substantial degree by surface tension or small movements or small mechanical vibrations. In one embodiment, the at least one wall is designed such that corners thereof are curved or are distant from the edge of the well to substantially eliminate wicking of liquid from the well. Also disclosed are methods for mixing materials on the surface of a support. A sample is incubated with the surface of the support of the aforementioned device. The sample is removed from the surface, a residual amount of the sample remaining on the surface. The surface is contacted with a wash solution. A stream of gas is directed at the surface of the support. The pressure of the stream and the angle of the stream create a vortex on the surface sufficient to mix the sample and the wash solution. The wash solution is then removed from the surface. Also disclosed are methods for conducting binding reactions using the devices of the invention.