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    Manufacture of arrays with varying deposition parameters
    2.
    发明授权
    Manufacture of arrays with varying deposition parameters 失效
    制造具有不同沉积参数的阵列

    公开(公告)号：US07078505B2

    公开(公告)日：2006-07-18

    申请号：US10165783

    申请日：2002-06-06

    申请人： Jay K. Bass ,  Maryam Mobed-Miremadi ,  Michelle M. Maranowski ,  Roy H. Kanemoto ,  Bill J. Peck ,  Eric M. Leproust ,  Michael P. Caren

    发明人： Jay K. Bass ,  Maryam Mobed-Miremadi ,  Michelle M. Maranowski ,  Roy H. Kanemoto ,  Bill J. Peck ,  Eric M. Leproust ,  Michael P. Caren

    IPC分类号： C07H21/04 ,  C12Q1/68 ,  C12M1/36

    CPC分类号： C03C17/32 ,  B01J2219/00286 ,  B01J2219/00378 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00698 ,  B01J2219/00722 ,  B01L3/0241 ,  C03C17/002 ,  C40B40/06 ,  C40B60/14

    摘要： Methods are disclosed for synthesizing a plurality of compounds on the surface of supports. The synthesis comprises a series of cycles of steps in which reagents for conducting the synthesis are deposited on the surface of the support to form the chemical compounds. At least one physical parameter of the deposition varies between cycles The method comprises conducting the synthesis in at least two sets of cycles, arbitrarily designated as a first set and a second set. Each cycle comprises at least one step of depositing reagents on a surface by means of droplet dispensing elements that traverse the surface of the support. At least one, and desirably all, of the following deposition parameters are employed as indicated: The number of times of repetition for a step of depositing reagents in at least one cycle of the second set is less than the number of times of repetition for a corresponding step in at least one cycle of the first set. The rate of deposition in at least one cycle of the second set is slower than or equal to the rate of deposition in at least one cycle of the first set. The rate of traversal of the dispensing elements over the surface of the support in at least one cycle of the second set is greater than such rate of traversal in at least one cycle of the first set.

    摘要翻译： 公开了在载体表面上合成多种化合物的方法。 该合成包括一系列循环的步骤，其中用于进行合成的试剂沉积在载体的表面上以形成化合物。 沉积物的至少一个物理参数在循环之间变化。该方法包括以任意指定为第一组和第二组的至少两组循环进行合成。 每个循环包括至少一个步骤，其通过穿过载体表面的液滴分配元件在表面上沉积试剂。 如下所示使用以下沉积参数中的至少一个，期望地全部以下沉积参数：在第二组的至少一个循环中沉积试剂的步骤的重复次数小于第二组的重复次数 在第一组的至少一个循环中的对应步骤。 在第二组的至少一个循环中的沉积速率慢于或等于第一组的至少一个循环中的沉积速率。 在第二组的至少一个循环中，分配元件在支撑体表面上的遍历速率大于在第一组的至少一个循环中的穿过速率。

    Preparation of biopolymer arrays
    3.
    发明申请
    Preparation of biopolymer arrays 审中-公开
    生物聚合物阵列的制备

    公开(公告)号：US20080085511A1

    公开(公告)日：2008-04-10

    申请号：US11543369

    申请日：2006-10-05

    申请人： Bill J. Peck ,  Eric M. Leproust

    发明人： Bill J. Peck ,  Eric M. Leproust

    IPC分类号： C12Q1/68 ,  B41J2/01 ,  G06F19/00 ,  C12M3/00

    CPC分类号： C07K1/047 ,  B01J19/0046 ,  B01J2219/0036 ,  B01J2219/00378 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B82Y30/00

    摘要： Methods and apparatus are disclosed for preparing an array of biopolymer features on a substrate by initiating multiple cycles of drop deposition of biopolymer forming reagents to feature locations on the surface of the substrate. Each cycle comprises at least one step of deposition of biopolymer monomers or biopolymer subunits. For at least one of the biopolymer forming reagents and for at least a portion of the method, a dispensing protocol is employed wherein the biopolymer forming reagent is dispensed from a separate drop dispenser of a drop dispensing device and each drop dispenser comprises multiple nozzles. Multiple passes of the drop dispensing devices are employed for each cycle. In each cycle of the dispensing protocol drops of reagents are dispensed to feature locations from at least two different nozzles for each reagent in at least two different passes of a drop dispensing device. The cycles are repeated a sufficient number of times to prepare the array of biopolymer features.

    摘要翻译： 公开了用于通过引发多个循环的生物聚合物形成试剂的滴加沉积在基底表面上的特征位置来制备基底上生物聚合物特征阵列的方法和装置。 每个循环包括沉积生物聚合物单体或生物聚合物亚单元的至少一个步骤。 对于生物聚合物形成试剂和至少一部分方法中的至少一种，使用分配方案，其中生物聚合物形成试剂从滴剂分配装置的单独的液滴分配器分配，并且每个滴液分配器包括多个喷嘴。 每个循环采用多次滴液分配装置。 在分配方案的每个循环中，在液滴分配装置的至少两个不同通道中，每个试剂滴从多个不同喷嘴的至少两个不同喷嘴分配到特征位置。 循环重复足够次数以制备生物聚合物特征阵列。

    Array assay devices and methods for making and using the same
    5.
    发明申请
    Array assay devices and methods for making and using the same 审中-公开
    阵列测定装置及其制造和使用方法

    公开(公告)号：US20080085512A1

    公开(公告)日：2008-04-10

    申请号：US11544395

    申请日：2006-10-05

    申请人： Petula N. D'Andrade ,  Eric M. Leproust

    发明人： Petula N. D'Andrade ,  Eric M. Leproust

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M3/00

    CPC分类号： B01L3/508 ,  B01J2219/00619 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01L2200/0689 ,  B01L2300/0636 ,  B01L2300/0822 ,  B01L2300/161

    摘要： Aspects of the invention include methods for producing a backing element for use in an array assay device. An embodiment of the invention includes providing a precursor backing element, e.g., a solid support having a fluid retaining structure on a planar surface thereof, subjecting the precursor backing element to a solvent/plasma treatment step and then contacting the solvent/plasma treated backing element with a surface energy modification agent. Also provided are backing elements produced in accordance with embodiments of the invention, as well as array assay devices that include the backing elements. In addition, methods of using the array assay devices in analyte detection applications, as well as kits for use therein are provided.

    摘要翻译： 本发明的方面包括用于制备用于阵列测定装置的背衬元件的方法。 本发明的一个实施例包括提供前体背衬元件，例如在其平面表面上具有流体保持结构的固体支撑体，对前体背衬元件进行溶剂/等离子体处理步骤，然后使溶剂/等离子体处理的背衬元件 具有表面能改性剂。 还提供了根据本发明的实施方案生产的背衬元件以及包括背衬元件的阵列测定装置。 此外，提供了在分析物检测应用中使用阵列测定装置的方法以及用于其中的试剂盒。

    Silico design of chemical arrays
    8.
    发明申请
    Silico design of chemical arrays 审中-公开
    化学阵列的硅胶设计

    公开(公告)号：US20070021919A1

    公开(公告)日：2007-01-25

    申请号：US11185207

    申请日：2005-07-19

    申请人： Eric M. Leproust ,  Michael G. Booth

    发明人： Eric M. Leproust ,  Michael G. Booth

    IPC分类号： G06F19/00

    CPC分类号： G16B25/00 ,  G16B50/00

    摘要： A system is described which comprises an input manager for receiving probe request information from a probe requester and a processing module configured to identify a probe sequence associated with the probe request information, wherein the processing module associates a flag with one or more nucleic acid sequences corresponding to the probe request associated with a predefined sequence characteristic. Methods of using the system and computer program products for implementing the methods are also described.

    摘要翻译： 描述了一种系统，其包括用于从探测请求者接收探测请求信息的输入管理器和被配置为识别与探测请求信息相关联的探测序列的处理模块，其中所述处理模块将标记与相应的一个或多个核酸序列相关联 到与预定义的序列特征相关联的探测请求。 还描述了使用系统和计算机程序产品来实现该方法的方法。

    Apparatuses and methods for oligonucleotide preparation
    9.
    发明授权
    Apparatuses and methods for oligonucleotide preparation 有权
    寡核苷酸制备的设备和方法

    公开(公告)号：US08415138B2

    公开(公告)日：2013-04-09

    申请号：US11469405

    申请日：2006-08-31

    申请人： Eric M. Leproust

    发明人： Eric M. Leproust

    IPC分类号： C12M1/00 ,  C12Q1/68 ,  C07H19/00 ,  C07H21/00 ,  B01J10/00 ,  B01J19/00 ,  B01J19/18

    CPC分类号： C07H21/00

    摘要： Provided are improved processes and apparatuses for solid phase oligonucleotide synthesis, the improvements comprising carrying out detritylation of the nascent oligonucleotide using a composition of an organic solvent, a protic solvent and a selected concentration, or selected concentration range, of water. In some embodiments, an oligonucleotide synthesis apparatus includes means for adding water to a detritylation reagent. In some embodiments, an apparatus can include a water detector for analyzing the water concentration of a detritylation reagent to be reacted with a nascent oligonucleotide. An apparatus can comprise a feedback loop to control the concentration of water at the point of the detritylation reaction and/or to control the detritylation reaction time. The apparatuses and methods reduce batch-to-batch variations in the manufacture of oligonucleotides immobilized on the surface of various substrates.

    摘要翻译： 提供了用于固相寡核苷酸合成的改进的方法和装置，其改进包括使用有机溶剂，质子溶剂和所选浓度或选定浓度范围的水进行新生寡核苷酸的去结合。 在一些实施方案中，寡核苷酸合成装置包括向去离子化试剂中加入水的装置。 在一些实施例中，装置可以包括用于分析与新生寡核苷酸反应的去离子试剂的水浓度的水检测器。 装置可以包括反馈回路以控制在去离子化反应的点处的水的浓度和/或控制不稳定反应时间。 这些装置和方法减少了固定在各种底物表面上的寡核苷酸制造中的批次间变化。

    Chemical Arrays and Methods of Using the Same
    10.
    发明申请
    Chemical Arrays and Methods of Using the Same 有权
    化学数组及其使用方法

    公开(公告)号：US20110230371A1

    公开(公告)日：2011-09-22

    申请号：US13116998

    申请日：2011-05-26

    申请人： Mel N. Kronick ,  Eric M. Leproust

    发明人： Mel N. Kronick ,  Eric M. Leproust

    IPC分类号： C40B40/04 ,  C40B50/14

    CPC分类号： C40B30/04 ,  G01N33/54366

    摘要： Methods and compositions for generating mixtures of product molecules from an initial chemical array are provided. In the subject methods, a chemical array of surface immobilized first moieties is subjected to cleavage conditions such that a composition of solution phase first moieties is produced. The resultant composition of solution phase first moieties is then contacted with one or more reactants to produce a mixture of product molecules that are different from the first moieties. Also provided are the arrays employed in the subject methods and kits for practicing the subject methods.

    摘要翻译： 提供了从初始化学阵列产生产物分子的混合物的方法和组合物。 在本发明方法中，表面固定的第一部分的化学阵列经受切割条件，使得产生溶液相第一部分的组合物。 然后将所得到的溶液相第一部分的组合物与一种或多种反应物接触以产生不同于第一部分的产物分子的混合物。 还提供了用于实践本发明方法的主题方法和试剂盒中使用的阵列。