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    METHOD AND ARRANGEMENT FOR CALIBRATING A SENSOR ELEMENT
    2.
    发明申请
    METHOD AND ARRANGEMENT FOR CALIBRATING A SENSOR ELEMENT 审中-公开
    用于校准传感器元件的方法和装置

    公开(公告)号：US20100248243A1

    公开(公告)日：2010-09-30

    申请号：US12743316

    申请日：2008-10-29

    申请人： Katja Friedrich ,  Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel ,  Renee Weber

    发明人： Katja Friedrich ,  Walter Gumbrecht ,  Peter Paulicka ,  Manfred Stanzel ,  Renee Weber

    IPC分类号： C12Q1/68 ,  C12M1/40

    CPC分类号： C12Q1/6837 ,  C12Q2527/107

    摘要： A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p] Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)

    摘要翻译： 公开了一种用于校准具有固定化探针寡核苷酸的传感器元件的方法，通过该探针寡​​核苷酸可以通过传感器检测靶核酸（Z）的结合。 在至少一个实施方案中，该方法包括：a）使传感器元件与对照核酸（K）接触，其熔解温度Tm（K）小于靶核酸的解链温度Tm（Z） 酸（Z）; b）在温度T [p] Tm（K）并且在温度T [n]处检测负控制信号。 根据本发明的至少一个实施方案的改进，在测量温度T [mess]测量目标核酸（Z）的测量信号，其中Tm（K）

    AUTOMATED CTC DETECTION
    4.
    发明申请
    AUTOMATED CTC DETECTION 审中-公开
    自动CTC检测

    公开(公告)号：US20140106388A1

    公开(公告)日：2014-04-17

    申请号：US14118701

    申请日：2012-04-13

    申请人： Joachim Bangert ,  Katja Friedrich ,  Walter Gumbrecht ,  Karsten Hiltawsky ,  Peter Paulicka ,  Manfred Stanzel

    发明人： Joachim Bangert ,  Katja Friedrich ,  Walter Gumbrecht ,  Karsten Hiltawsky ,  Peter Paulicka ,  Manfred Stanzel

    IPC分类号： G01N33/50

    CPC分类号： G01N33/5091 ,  C12Q1/6806 ,  G01N1/30

    摘要： An embodiment relates to a method and to an array for detecting live circulating or disseminated cells in body fluids (for example blood, urine) or tissue samples (for example bone marrow) mixed with liquid. The method includes: a) filtering the liquid sample through a porous membrane that is suitable for retaining cells to be detected, such that cells to be detected come to rest upon at least a part of the surface of the membrane and the sample liquid passes the membrane; b) applying a first process liquid containing a first agent for marking the cells to be detected with a first marker; c) incubating the process liquid on the membrane for a predetermined time period, wherein the cells to be detected are marked; and d) detecting the marked cells to be detected on the surface of the membrane.

    摘要翻译： 实施方案涉及用于检测与液体混合的体液（例如血液，尿液）或组织样品（例如骨髓）中的活的循环或播散细胞的方法和阵列。 该方法包括：a）通过适于保留待检测细胞的多孔膜过滤液体样品，使待检测细胞停留在膜表面的至少一部分上，样品液体通过 膜; b）施加含有用于用第一标记标记待检测细胞的第一试剂的第一处理液; c）将所述工艺液体在所述膜上孵育预定时间段，其中所述待检测的细胞被标记; 以及d）检测在膜的表面上待检测的标记细胞。