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公开(公告)号：US5318680A
公开(公告)日：1994-06-07
申请号：US847176
申请日：1992-03-06
CPC分类号： G01N30/6039 , G01N27/44717 , G01N27/44773 , G01N30/16 , G01N2030/027 , G01N2030/162 , G01N2030/285 , G01N2030/8435 , G01N30/02 , G01N30/6034
摘要： An on-column derivatization scheme where a liquid or solid labeling reagent is provided in a capillary to react chemically with a sample in order to label the sample. Electrophoretic separation may be carried out immediately thereafter to simplify the derivatization, separation and detection process. By localizing the labeling process to a small area within the capillary, fast kinetics and high reaction yield are achieved.
摘要翻译： 柱内衍生方案，其中在毛细管中提供液体或固体标记试剂以与样品进行化学反应以标记样品。 此后可以进行电泳分离，以简化衍生化，分离和检测过程。 通过将标记过程定位在毛细管内的一个小区域，实现了快速动力学和高反应产率。
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2.发明授权Method and device employing time-delayed integration for detecting sample components after separation 失效方法和装置采用时间延迟积分检测分离后的样品成分
公开(公告)号：US5141609A
公开(公告)日：1992-08-25
申请号：US614830
申请日：1990-11-16
IPC分类号： G01J3/28 , G01N27/447 , G01N30/62 , G01N30/74 , G01N30/86
CPC分类号： G01N30/74 , G01N27/44721 , G01N30/8603 , G01J3/2803 , G01N2021/6417 , G01N2030/621 , G01N21/6408 , G01N30/8606
摘要： This invention relates to a detection method and apparatus useful in capillary electrophoresis and capillary chromatography that employs an array of solid state detector such as a charge-coupled device operating in the time-delayed integration mode which allows more exposure time of the moving analyte zones. The CCD is synchronized so that after a normal exposure of the CCD, the charge information in every row of the CCD is shifted toward one end of the CCD and the charge/signal information in the last row is quantified. Applying the CCD and the time-delayed integration method in effect increases the effective sampling volume of the flow cell without introducing band broadening. Use of the CCD as a fluorescence detection in capillary electrophoresis separations allows analytes to be differentiated both in migration time and fluorescence emission, yielding detection limits for fluorophores in the 1-8.times.10.sup.-20 mole range.
摘要翻译： 本发明涉及一种用于毛细管电泳和毛细管色谱法的检测方法和装置，其采用固态检测器阵列，例如以时间延迟积分模式工作的电荷耦合器件，其允许移动的分析物区域的更多的暴露时间。 CCD被同步，使得在CCD的正常曝光之后，CCD的每行中的电荷信息向CCD的一端移动，并且对最后一行的充电/信号信息进行量化。 应用CCD和时间延迟积分法有效地增加了流动池的有效采样体积，而不引入带宽。 在毛细管电泳分离中使用CCD作为荧光检测可以使分析物在迁移时间和荧光发射中均有区别，从而产生1-8x10-20摩尔范围的荧光团的检测限。
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3.发明授权
公开(公告)号：US5654636A
公开(公告)日：1997-08-05
申请号：US706924
申请日：1996-09-03
IPC分类号： G01R33/46
CPC分类号： G01R33/46
摘要： A method and apparatus for structural elucidation and determination of a nanoliter size sample of analyte. The method includes separating the analyte using a microseparation technique and then substantially simultaneously analyzing or detecting the sample using a nuclear magnetic resonance (NMR) spectrometer. The apparatus includes an RF microcoil positioned for operable communication with a capillary member of the microseparation system and the NMR spectrometer to provide enhanced sensitivity and improved signal-to-noise ratio of the NMR spectrometer necessary to accurately analyze or detect nanoliter size samples.
摘要翻译： 用于结构阐明和测定分析物的纳升尺寸样品的方法和装置。 该方法包括使用微分离技术分离分析物，然后使用核磁共振（NMR）光谱仪基本上同时分析或检测样品。 该装置包括被定位用于与微分离系统的毛细管构件和NMR光谱仪可操作连通的RF微线圈，以提供精确分析或检测纳升尺寸样品所需的NMR光谱仪的增强的灵敏度和改善的信噪比。
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公开(公告)号：US07220345B2
公开(公告)日：2007-05-22
申请号：US10273935
申请日：2002-10-18
IPC分类号： B67D5/00
CPC分类号： B01D57/02 , B01J19/0093 , B01J2219/00783 , B01J2219/00907 , B01J2219/0093 , B01L3/502707 , B01L3/502761 , B01L2200/027 , B01L2300/0681 , B01L2300/0874 , B01L2300/0896 , B01L2400/0415 , B01L2400/06 , B01L2400/0627 , B82Y30/00 , G01N27/4473 , G01N27/44791
摘要： A fluid circuit includes a membrane having a first side, a second side opposite the first side, and a pore extending from the first side to the second side. The circuit also includes a first channel containing fluid extending along the first side of the membrane and a second channel containing fluid extending along the second side of the membrane and crossing the first channel. The circuit also includes an electrical source in electrical communication with at least one of the first fluid and second fluid for selectively developing an electrical potential between fluid in the first channel and fluid in the second channel. This causes at least one component of fluid to pass through the pore in the membrane from one of the first channel and the second channel to the other of the first channel and the second channel.
摘要翻译： 流体回路包括具有第一侧，与第一侧相对的第二侧和从第一侧向第二侧延伸的孔的膜。 电路还包括含有沿着膜的第一侧延伸的流体的第一通道和包含沿膜的第二侧延伸并与第一通道交叉的流体的第二通道。 电路还包括与第一流体和第二流体中的至少一个电连通的电源，用于选择性地在第一通道中的流体和第二通道中的流体之间形成电势。 这使得流体的至少一个成分从第一通道和第二通道中的一个通过膜中的孔到第一通道和第二通道中的另一个。
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5.发明授权公开(公告)号：US06762134B2
公开(公告)日：2004-07-13
申请号：US09989050
申请日：2001-11-20
IPC分类号： H01L21302
CPC分类号： H01L21/30612 , H01L21/0332 , H01L21/3081 , H01L33/18 , H01L33/30
摘要： A thin discontinuous layer of metal such as Au, Pt, or Au/Pd is deposited on a Group III-V material surface. The surface is then etched in a solution including HF and an oxidant for a preferably brief period, as little as a couple seconds to one hour. A preferred oxidant is H2O2. Morphology and light emitting properties of porous Group III-V material can be selectively controlled as a function of the type of metal deposited, doping type, doping level, metal thickness, whether emission is collected on or off the metal coated areas and/or etch time. Electrical assistance is unnecessary during the chemical etching of the invention, which may be conducted in the presence or absence of illumination.
摘要翻译： 在III-V族材料表面上沉积诸如Au，Pt或Au / Pd之类的金属薄的不连续层。 然后将表面蚀刻在包含HF和氧化剂的溶液中，优选短暂的时间，几小时至几小时。 优选的氧化剂是H 2 O 2。 可以选择性地控制多孔III-V族材料的形态和发光性质，作为金属沉积类型，掺杂类型，掺杂水平，金属厚度，是否在金属涂层区域或/或金属涂层区域上收集发射的函数的函数 时间。 在本发明的化学蚀刻期间不需要电辅助，其可以在存在或不存在照明的情况下进行。
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公开(公告)号：US6097188A
公开(公告)日：2000-08-01
申请号：US381230
申请日：1995-01-31
CPC分类号： G01R33/5604
摘要： The present invention provides an NMR apparatus comprising an analyte sample holder having a containment region that holds a volume of less than about 10 microliters of the analyte sample, a microcoil, which encloses the containment region of the analyte sample holder and the analyte sample contained therein, the microcoil having an inside dimension of less than about 1 mm, and the microcoil operatively associated with the analyte sample contained in the containment region of the analyte sample holder such that the microcoil can transmit and/or receive energy from the analyte sample in the containment region of the analyte sample holder, and a magnet having a mass less than about 50 kg, the magnet positioned about the microcoil and the analyte sample in the containment region of the analyte sample holder to provide a static magnetic field in which the microcoil and the analyte sample in the containment region of the analyte sample holder are located, wherein the microcoil and the magnet provide for the obtainment of an NMR spectrum. The present invention also provides a method of analysis and elucidation of the chemical structure of an analyte using NMR, the method comprising providing an analyte sample that has a volume of less than about 10 microliters, enclosing the analyte sample in a microcoil, generating a static magnetic field about the analyte sample using a magnet, transmitting energy to the analyte sample to excite the sample from its equilibrium state to its excited state, detecting the energy emitted from the analyte sample when the sample relaxes from its excited state to its equilibrium state using the microcoil, and analyzing and graphically displaying the detected energy as a time or frequency domain spectrum.
摘要翻译： 本发明提供一种NMR装置，其包括分析物样品保持器，其具有容纳小于约10微升分析物样品的容纳区域，包围分析物样品架的容纳区域和其中包含的分析物样品的微线圈 ，所述微线圈具有小于约1mm的内部尺寸，并且所述微线圈与分析物样品保持器的容纳区域中包含的分析物样品可操作地相关联，使得所述微线圈可以从所述分析物样品中传输和/或接收能量 分析物样品保持器的容纳区域和质量小于约50kg的磁体，磁体围绕微线圈和分析物样品位于分析物样品保持器的容纳区域中，以提供静态磁场，其中微线圈和 位于分析物样品架的容纳区域中的分析物样品，其中微线圈和磁体 提供获得NMR光谱。 本发明还提供了使用NMR分析和分析分析物的化学结构的方法，所述方法包括提供具有小于约10微升的体积的分析物样品，将分析物样品包封在微线圈中，产生静电 使用磁体关于分析物样品的磁场，将能量传递到分析物样品以将样品从其平衡态激发到其激发态，当样品从其激发态松弛至其平衡状态时，检测从分析物样品发射的能量，使用 微线圈，并分析和图形显示检测到的能量作为时域或频域光谱。
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7.发明授权Method and apparatus for simultaneous acquisition of high resolution NMR spectra from multiple samples 有权用于从多个样品同时获取高分辨率NMR光谱的方法和装置公开(公告)号：US06456072B1
公开(公告)日：2002-09-24
申请号：US09579827
申请日：2000-05-26
IPC分类号： G01V300
CPC分类号： G01R33/30
摘要： An apparatus for obtaining multiple spectra from a plurality of samples through the use of nuclear magnetic resonance (NMR) spectroscopy. A plurality of microcoils are coupled to and are in operable communication with an NMR spectrometer. The coils are operable in both a transmit mode and a receive mode to transmit RF energy to a sample being analyzed and to receive the return data for analysis by the NMR spectrometer. At least one switch is coupled to the plurality of coils and the NMR spectrometer. A controller for the NMR spectrometer is coupled to the switch and is used to control alternative activation of the coils for NMR data acquisition of multiple samples.
摘要翻译： 一种用于通过使用核磁共振（NMR）光谱从多个样品获得多个光谱的装置。 多个微线圈耦合到NMR光谱仪并与之可操作地连通。 线圈可以在发射模式和接收模式两者中操作，以将RF能量发送到正在分析的样本，并接收用于通过NMR光谱仪进行分析的返回数据。 至少一个开关耦合到多个线圈和NMR光谱仪。 用于NMR光谱仪的控制器耦合到开关，并且用于控制用于多个样品的NMR数据采集的线圈的替代激活。
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8.发明授权
公开(公告)号：US5684401A
公开(公告)日：1997-11-04
申请号：US595269
申请日：1996-02-01
CPC分类号： G01R33/56536 , G01R33/5604 , G01R33/302 , G01R33/34053 , G01R33/341
摘要： An apparatus and method for compensation of magnetic susceptibility variation in NMR microspectroscopy detection microcoils. NMR detection microcoils are formed with side-by-side windings in order to approximate a uniform cylindrical sheet coil. The wire may have a thin layer of insulation in order to prevent shorting between adjacent turns, or (if the wire diameter is small enough) uninsulated wire may be wound with the adjacent turns touching without fear of shorting between coil turns. Additionally, a susceptibility matching medium, such as a perfluorinated hydrocarbon, may be placed around the microcoil to minimize susceptibility-induced variations in the B.sub.0 magnetic field.
摘要翻译： NMR显微光谱检测微线圈磁化率变化的装置和方法。 NMR检测微线圈由并排绕组形成，以便近似均匀的圆柱形片状线圈。 导线可以具有薄的绝缘层，以防止相邻匝之间的短路，或者（如果导线直径足够小），非绝缘线可以在相邻匝之间缠绕而不用担心线圈匝之间的短路。 此外，易位性匹配介质，例如全氟化烃，可放置在微线圈周围，以最小化磁化率诱导的B0磁场变化。
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公开(公告)号：US06790785B1
公开(公告)日：2004-09-14
申请号：US09662682
申请日：2000-09-15
申请人： Xiuling Li , Paul W. Bohn , Jonathan V. Sweedler
发明人： Xiuling Li , Paul W. Bohn , Jonathan V. Sweedler
IPC分类号： H01L21302
CPC分类号： H01L21/0203
摘要： A thin discontinuous layer of metal such as Au, Pt, or Au/Pd is deposited on a silicon surface. The surface is then etched in a solution including HF and an oxidant for a brief period, as little as a couple seconds to one hour. A preferred oxidant is H2O2. Morphology and light emitting properties of porous silicon can be selectively controlled as a function of the type of metal deposited, Si doping type, silicon doping level, and/or etch time. Electrical assistance is unnecessary during the chemical etching of the invention, which may be conducted in the presence or absence of illumination.
摘要翻译： 金属如Au，Pt或Au / Pd的薄的不连续层沉积在硅表面上。 然后将表面在包含HF和氧化剂的溶液中蚀刻短暂时间，只需几秒至1小时。 优选的氧化剂是H 2 O 2。 可以选择性地控制多孔硅的形态和发光性质，作为沉积金属，Si掺杂类型，硅掺杂水平和/或蚀刻时间的函数。 在本发明的化学蚀刻期间不需要电辅助，其可以在存在或不存在照明的情况下进行。
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公开(公告)号：US06788061B1
公开(公告)日：2004-09-07
申请号：US10252655
申请日：2002-09-23
IPC分类号： G01V300
CPC分类号： G01R33/302 , G01R33/46
摘要： The present invention provides and NMR apparatus comprising an analyte sample holder having a containment region that holds a volume of less than about 1 microliters of the analyte sample, a coil, which encloses the containment region of the analyte sample holder and the analyte sample contained therein, the coil operatively associated with the analyte sample contained in the containment region of the analyte sample holder such that the coil can transmit and/or receive energy from the analyte sample in the containment region of the analyte sample holder, and a magnet to provide a static magnetic field in which the coil and the analyte sample in the containment region of the analyte sample holder are located, wherein the coil and the magnet provide for the obtainment of an NMR spectrum having a spectral line width resolution of less than about 0.1 parts per million.
摘要翻译： 本发明提供了包括具有容纳区域的分析物样品保持器的NMR装置，所述容纳区域保持小于约1微升的分析物样品的体积，包围分析物样品保持器的容纳区域和其中包含的分析物样品的线圈 ，所述线圈与分析物样品保持器的容纳区域中包含的分析物样品可操作地相关联，使得线圈可以从分析物样品保持器的容纳区域中的分析物样品和磁体传输和/或接收能量，以提供 其中线圈和分析物样品架的容纳区域中的分析物样品位于其中的静磁场，其中线圈和磁体提供获得具有小于约0.1份的谱线宽度分辨率的NMR光谱 百万。
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