公开(公告)号：US20090263870A1

公开(公告)日：2009-10-22

申请号：US12428901

申请日：2009-04-23

申请人： Juergen Pipper ,  Pavel Neuzil ,  Yi Zhang ,  Muhammad Khidhir Khairudin ,  Tseng-Ming Hsieh

发明人： Juergen Pipper ,  Pavel Neuzil ,  Yi Zhang ,  Muhammad Khidhir Khairudin ,  Tseng-Ming Hsieh

IPC分类号： C12P19/34 ,  C12M1/00

CPC分类号： C12P19/34 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2563/137

摘要： There is provided a method and/or system which allow on-chip preconditioning of complex real-world samples and/or handling of limited amounts of target material, and/or on-chip nucleic acid amplification process, using a free droplet containing magnetic attractable material. The nucleic acid amplification process comprises controlling the position of the magnetic attractable material and performing the nucleic acid amplification in a thermocycling droplet located onto at least one temperature zone. The low thermal masses of the herein described heaters/temperature sensors come along with fast temperature transitions within the corresponding temperature zones allowing impressing temperature gradients in at least one temperature zone between subsequent or within the same thermocycle(s). Additionally, the variable residence times of the droplet in a given temperature zone permit to customize the denaturation, annealing and/or extension times within the same or between different PCR runs. Additionally, the herein described method and/or system allow amplification of one or more nucleic acid molecules. Additionally, the herein described method and/or system allow real-time monitoring with or without the presence of magnetic attractable material bound to said nucleic acid molecule.

摘要翻译： 提供了一种方法和/或系统，其允许使用包含磁性可吸引的自由液滴来对复杂的现实世界样品进行片上预处理和/或处理有限量的靶材料和/或片上核酸扩增过程 材料。 核酸扩增方法包括控制磁性可吸引材料的位置并在位于至少一个温度区域上的热循环液滴中进行核酸扩增。 本文描述的加热器/温度传感器的低热质量伴随着相应温度区域内的快速温度转变，允许在相同或相同的热循环之间的至少一个温度区域内施加温度梯度。 此外，液滴在给定温度区域的可变停留时间允许在相同或不同PCR运行之间定制变性，退火和/或延伸时间。 另外，本文所述的方法和/或系统允许扩增一个或多个核酸分子。 另外，本文描述的方法和/或系统允许使用或不存在与所述核酸分子结合的可磁性吸引材料的实时监测。

公开(公告)号：US08124033B2

公开(公告)日：2012-02-28

申请号：US11356511

申请日：2006-02-17

申请人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

发明人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

IPC分类号： B01L9/00

CPC分类号： B01L3/5088 ,  B01J2219/00495 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01N21/0332 ,  G01N25/18

摘要： The invention provides an apparatus for regulating the temperature of a chemical and/or biological sample and a method of using the same. The apparatus includes at least one temperature control module. The temperature control module includes a heater, a conductor of caloric, and a temperature sensor. The heater of the temperature control module is adapted to thermally communicate with a removable substrate, on which said chemical and/or biological sample is placed, via the conductor of caloric. The temperature sensor of the temperature control module is adapted to detect and control the temperature of the substrate via the conductor of caloric. The apparatus is designed such that the substrate is situated above said temperature control module to entirely cover said temperature control module.

摘要翻译： 本发明提供了一种用于调节化学和/或生物样品的温度的装置及其使用方法。 该装置包括至少一个温度控制模块。 温度控制模块包括加热器，热量导体和温度传感器。 温度控制模块的加热器适于通过热量导体与其上放置所述化学和/或生物样品的可移除基板热连通。 温度控制模块的温度传感器适于通过热量导体检测和控制基板的温度。 设备被设计成使得基板位于所述温度控制模块上方以完全覆盖所述温度控制模块。

公开(公告)号：US20070196237A1

公开(公告)日：2007-08-23

申请号：US11356511

申请日：2006-02-17

申请人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

发明人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

IPC分类号： G01N31/00

CPC分类号： B01L3/5088 ,  B01J2219/00495 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01N21/0332 ,  G01N25/18

摘要： The invention provides an apparatus for regulating the temperature of a chemical and/or biological sample and a method of using the same. The apparatus includes at least one temperature control module. The temperature control module includes a heater, a conductor of caloric, and a temperature sensor. The heater of the temperature control module is adapted to thermally communicate with a removable substrate, on which said chemical and/or biological sample is placed, via the conductor of caloric. The temperature sensor of the temperature control module is adapted to detect and control the temperature of the substrate via the conductor of caloric. The apparatus is designed such that the substrate is situated above said temperature control module to entirely cover said temperature control module.

摘要翻译： 本发明提供了一种用于调节化学和/或生物样品的温度的装置及其使用方法。 该装置包括至少一个温度控制模块。 温度控制模块包括加热器，热量导体和温度传感器。 温度控制模块的加热器适于通过热量导体与其上放置所述化学和/或生物样品的可移除基板热连通。 温度控制模块的温度传感器适于通过热量导体检测和控制基板的温度。 设备被设计成使得基板位于所述温度控制模块上方以完全覆盖所述温度控制模块。

公开(公告)号：US20120244605A1

公开(公告)日：2012-09-27

申请号：US13346418

申请日：2012-01-09

申请人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

发明人： Pavel Neuzil ,  Tseng-Ming Hsieh ,  Juergen Pipper

IPC分类号： C12M1/38 ,  G01N1/28

CPC分类号： B01L3/5088 ,  B01J2219/00495 ,  B01L3/5027 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01N21/0332 ,  G01N25/18

摘要： The invention provides an apparatus for regulating the temperature of a chemical and/or biological sample and a method of using the same. The apparatus includes at least one temperature control module. The temperature control module includes a heater, a conductor of caloric, and a temperature sensor. The heater of the temperature control module is adapted to thermally communicate with a removable substrate, on which said chemical and/or biological sample is placed, via the conductor of caloric. The temperature sensor of the temperature control module is adapted to detect and control the temperature of the substrate via the conductor of caloric. The apparatus is designed such that the substrate is situated above said temperature control module to entirely cover said temperature control module.

摘要翻译： 本发明提供了一种用于调节化学和/或生物样品的温度的装置及其使用方法。 该装置包括至少一个温度控制模块。 温度控制模块包括加热器，热量导体和温度传感器。 温度控制模块的加热器适于通过热量导体与其上放置所述化学和/或生物样品的可移除基板热连通。 温度控制模块的温度传感器适于通过热量导体检测和控制基板的温度。 设备被设计成使得基板位于所述温度控制模块上方以完全覆盖所述温度控制模块。

公开(公告)号：US20090289213A1

公开(公告)日：2009-11-26

申请号：US12279308

申请日：2006-02-13

申请人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

发明人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

IPC分类号： H01F1/00

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/28009 ,  B01J2220/54 ,  B03C1/01 ,  C12M25/01 ,  G01N33/5432 ,  G01N33/5436 ,  G01N2446/00

摘要： The invention provides a method of processing a biological and/or chemical sample. The method includes providing a fluid droplet, which includes an inner phase and an outer phase. The outer phase is immiscible with the inner phase, and the outer phase is surrounding the inner phase. The inner phase includes the biological and/or chemical sample. The fluid droplet furthermore comprises magnetically attractable matter. The method also includes providing at least one surface, which is of such a texture and such a wettability for the fluid of the inner phase of the fluid droplet, that the fluid droplet remains intact upon being contacted therewith. The method further includes disposing the fluid droplet onto the at least one surface. The method also includes performing a process on the biological and/or chemical sample in the fluid droplet.

摘要翻译： 本发明提供了一种处理生物和/或化学样品的方法。 该方法包括提供包括内相和外相的流体液滴。 外相与内相不混溶，外相围绕内相。 内相包括生物和/或化学样品。 流体液滴还包括磁吸引物质。 该方法还包括提供至少一个表面，其具有这样的质地和对流体液滴的内相的流体的润湿性，使得流体液滴在与其接触时保持完整。 该方法还包括将流体液滴设置在至少一个表面上。 该方法还包括对流体液滴中的生物和/或化学样品进行处理。

公开(公告)号：US08216855B2

公开(公告)日：2012-07-10

申请号：US12279308

申请日：2006-02-13

申请人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

发明人： Juergen Pipper ,  Tseng-Ming Hsieh ,  Pavel Neuzil

IPC分类号： G01N33/553

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/28009 ,  B01J2220/54 ,  B03C1/01 ,  C12M25/01 ,  G01N33/5432 ,  G01N33/5436 ,  G01N2446/00

摘要： The invention provides a method of processing a biological and/or chemical sample. The method includes providing a fluid droplet, which includes an inner phase and an outer phase. The outer phase is immiscible with the inner phase, and the outer phase is surrounding the inner phase. The inner phase includes the biological and/or chemical sample. The fluid droplet furthermore comprises magnetically attractable matter. The method also includes providing at least one surface, which is of such a texture and such a wettability for the fluid of the inner phase of the fluid droplet, that the fluid droplet remains intact upon being contacted therewith. The method further includes disposing the fluid droplet onto the at least one surface. The method also includes performing a process on the biological and/or chemical sample in the fluid droplet.

摘要翻译： 本发明提供了一种处理生物和/或化学样品的方法。 该方法包括提供包括内相和外相的流体液滴。 外相与内相不混溶，外相围绕内相。 内相包括生物和/或化学样品。 流体液滴还包括磁吸引物质。 该方法还包括提供至少一个表面，其具有这样的质地和对流体液滴的内相的流体的润湿性，使得流体液滴在与其接触时保持完整。 该方法还包括将流体液滴设置在至少一个表面上。 该方法还包括对流体液滴中的生物和/或化学样品进行处理。

公开(公告)号：US20130136671A1

公开(公告)日：2013-05-30

申请号：US13696063

申请日：2011-05-04

申请人： Mo-Huang Li ,  Jackie Y. Yang ,  Guolin Xu ,  Yoke San Daniel Lee ,  Emril Mohamed Ali ,  Tseng-Ming Hsieh

发明人： Mo-Huang Li ,  Jackie Y. Yang ,  Guolin Xu ,  Yoke San Daniel Lee ,  Emril Mohamed Ali ,  Tseng-Ming Hsieh

IPC分类号： B01L3/00 ,  B01L3/02

CPC分类号： B01L3/52 ,  F04F1/18

摘要： According to various embodiments, a reagent fluid dispensing device may be provided. The reagent fluid dispensing device may include a chamber for receiving a reagent fluid, the chamber having a first opening and a second opening; a first fluid conduit connected to the first opening of the chamber; a reservoir connected to the first fluid conduit, the reservoir having a first opening, wherein the first opening of the reservoir is connected to the first fluid conduit to form a passive valve, wherein the reservoir is dimensionalized for storing a predetermined volume of the reagent fluid; and a pneumatic conduit connected to the second opening of the chamber, wherein selective application of pneumatic pressure to the chamber through the pneumatic conduit transfers the reagent fluid from the reservoir to the chamber through the first fluid conduit. According to various embodiments, a microfluidic device including the reagent fluid dispensing device, and a method of dispensing a reagent fluid may be provided.

摘要翻译： 根据各种实施例，可以提供试剂流体分配装置。 试剂流体分配装置可以包括用于接收试剂流体的腔室，该腔室具有第一开口和第二开口; 连接到所述室的第一开口的第一流体导管; 连接到第一流体管道的储存器，储存器具有第一开口，其中储存器的第一开口连接到第一流体导管以形成被动阀，其中储存器被尺寸化以存储预定体积的试剂流体 ; 以及连接到所述腔室的第二开口的气动导管，其中通过所述气动导管选择性地将气压施加到所述腔室通过所述第一流体导管将所述试剂流体从所述储存器传送到所述腔室。 根据各种实施例，可以提供包括试剂流体分配装置的微流体装置和分配试剂流体的方法。

公开(公告)号：US20100248245A1

公开(公告)日：2010-09-30

申请号：US12746510

申请日：2008-11-06

申请人： Jackie Y. Ying ,  Tseng-Ming Hsieh ,  Jeremy Ming Hock Loh ,  Chunyan Zhang

发明人： Jackie Y. Ying ,  Tseng-Ming Hsieh ,  Jeremy Ming Hock Loh ,  Chunyan Zhang

IPC分类号： C12Q1/68 ,  C12M1/34 ,  C12M1/38

CPC分类号： B01L3/50851 ,  B01J2219/00283 ,  B01J2219/00306 ,  B01J2219/00495 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2300/0654 ,  B01L2300/1822 ,  B01L2300/1827 ,  G01N21/03 ,  G01N2021/0346

摘要： Apparatus for conducting and monitoring chemical reactions comprises a base and a thermal cycler mounted on the base. A plurality of heat-conducting receptacles are mounted on the thermal cycler and in heat-communication therewith. Each receptacle comprises an opaque body defining a bore having an open end, a first window, and a second window. A cartridge is removably mounted on the receptacles. The cartridge comprises a plurality of light-transmitting reaction vessels, and conduits connected to the reaction vessels for processing and transferring fluid. The reaction vessels are received in the bores of the receptacles through the open ends of the bores. A light emitter is mounted on the base for illuminating the reaction vessels through the first windows of the reaction vessels. A light detector is mounted on the base for selectively receiving and detecting light emitted from the reaction vessels through the second windows of the receptacles.

摘要翻译： 用于进行和监测化学反应的装置包括安装在基座上的底座和热循环仪。 多个导热容器安装在热循环仪上并与其热连通。 每个容器包括限定具有开口端，第一窗口和第二窗口的孔的不透明体。 容器可拆卸地安装在容器上。 盒包括多个透光反应容器和连接到反应容器的管道，用于处理和传送流体。 反应容器通过孔的开口端容纳在容器的孔中。 光发射器安装在基座上，用于通过反应容器的第一窗口照亮反应容器。 光检测器安装在基座上，用于通过容器的第二窗口选择性地接收和检测从反应容器发射的光。

公开(公告)号：US20100240117A1

公开(公告)日：2010-09-23

申请号：US12749831

申请日：2010-03-30

申请人： Jackie Y. Ying ,  Shyi Herng Kan ,  Jeremy Ming Hock Loh ,  Karl Schumacher ,  Tseng-Ming Hsieh

发明人： Jackie Y. Ying ,  Shyi Herng Kan ,  Jeremy Ming Hock Loh ,  Karl Schumacher ,  Tseng-Ming Hsieh

IPC分类号： C12M3/00 ,  C12M1/00

CPC分类号： A61L27/38 ,  A61L27/56 ,  B33Y80/00 ,  C12N5/0068 ,  C12N2533/30

摘要： Methods and apparatuses involving biocompatible structures for tissue engineering and organ replacement and, more specifically, biocompatible structures formed by three-dimensional fabrication, are described. In some embodiments, the biocompatible structures are scaffolds for cells that can be used as tissue engineering templates and/or as artificial organs. The structures may be three-dimensional and can mimic the shapes and dimensions of tissues and/or organs, including the microarchitecture and porosities of the tissues and organs. Pores in the structure may allow delivery of molecules across the structure, and may facilitate cell migration and/or generation of connective tissue between the structure and its host environment. Structures of the invention can be implanted into a mammal and/or may be used ex vivo as bioartificial assist devices.

摘要翻译： 描述了涉及用于组织工程和器官置换的生物相容性结构的方法和装置，更具体地，涉及通过三维制造形成的生物相容性结构。 在一些实施方案中，生物相容性结构是可用作组织工程模板和/或作为人造器官的细胞的支架。 结构可以是三维的并且可以模拟组织和/或器官的形状和尺寸，包括组织和器官的微结构和孔隙率。 结构中的孔可以允许跨结构传递分子，并且可以促进细胞迁移和/或结构与其宿主环境之间的结缔组织的产生。 本发明的结构可以植入哺乳动物和/或作为生物人造辅助装置离体使用。

公开(公告)号：US20120228802A1

公开(公告)日：2012-09-13

申请号：US12669466

申请日：2008-07-21

申请人： Shyi-Herng Kan ,  Tseng-Ming Hsieh ,  Jackie Y. Ying

发明人： Shyi-Herng Kan ,  Tseng-Ming Hsieh ,  Jackie Y. Ying

IPC分类号： B29C35/08 ,  G02B27/30

CPC分类号： B29C64/135

摘要： A system for focusing a light beam may be used for multi-photon stereolithography. It comprises a collimator or expander for adjusting the beam divergence and a scanner for directing the beam onto a focusing device to focus the beam to a focal point or beam waist and to scan the focused beam. A controller controls adjustment of the beam divergence so that the focal point or beam waist is scanned substantially in a plane. A light source may be provided to generate the light beam. The expander may comprise a diverging lens and a converging lens for expanding the beam to produce a collimated beam. The divergence of the collimated beam is dependent on the distance between the diverging lens and the converging lens, which may be adjusted to adjust the beam divergence. The focusing device may comprise a dry objective lens to focus the collimated beam onto the target material to induce multi-photon absorption in the target material at the beam waist of the focused beam.

摘要翻译： 用于聚焦光束的系统可以用于多光子立体光刻。 它包括用于调整光束发散的准直器或扩展器和用于将光束引导到聚焦装置上以将光束聚焦到焦点或光束腰部并扫描聚焦光束的扫描器。 控制器控制光束发散的调节，使得焦点或光束腰部基本上在平面中扫描。 可以提供光源以产生光束。 扩展器可以包括发散透镜和用于展开光束以产生准直光束的会聚透镜。 准直光束的发散度取决于发散透镜和会聚透镜之间的距离，其可以被调整以调节光束发散。 聚焦装置可以包括干物镜，以将准直光束聚焦到目标材料上，以在聚焦光束的束腰处的目标材料中引起多光子吸收。