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1.发明授权High sensitivity fluorescent single particle and single molecule detection apparatus and method 失效高灵敏度荧光单粒子和单分子检测仪器及方法
公开(公告)号：US4979824A
公开(公告)日：1990-12-25
申请号：US358782
申请日：1989-05-26
申请人： Richard A. Mathies , Konan Peck , Lubert Stryer
发明人： Richard A. Mathies , Konan Peck , Lubert Stryer
IPC分类号： G01N21/63 , G01N15/02 , G01N15/14 , G01N21/64 , G01N33/543
CPC分类号： G01N21/645 , G01N15/147 , G01N15/1429 , G01N15/1475 , G01N2015/0222 , G01N21/6456
摘要： Apparatus is described for ultrasensitive detection of single fluorescent particles down to the single fluorescent molecule limit in a fluid or on a substrate comprising means for illuminating a predetermined volume of the fluid or area of the substrate whereby to emit light including background light from the fluid and burst of photons from particles residing in the area. The photon burst is detected in real time to generate output representative signal. The signal is received and the burst of energy from the fluorescent particles is distinguished from the background energy to provide an indication of the number, location or concentration of the particles or molecules.
摘要翻译： 描述了用于在流体或基板上的单个荧光分子极限处的超灵敏检测的装置，其包括用于照射预定体积的基板的流体或区域的装置，从而发射包括来自流体的背景光的光，以及 从位于该地区的颗粒中发射光子。 实时检测光子脉冲以产生输出代表信号。 信号被接收，并且来自荧光颗粒的能量爆发与背景能区别开来，以提供颗粒或分子的数量，位置或浓度的指示。
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2.发明授权公开(公告)号：US06428667B1
公开(公告)日：2002-08-06
申请号：US09686147
申请日：2000-10-10
IPC分类号： B01D5702
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747 , Y10T436/143333 , C12Q2565/102 , C12Q2563/173
摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。
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公开(公告)号：US5763162A
公开(公告)日：1998-06-09
申请号：US161231
申请日：1993-12-02
IPC分类号： C07D221/12 , C07D405/12 , C07D417/06 , C07D417/14 , C09B23/04 , C12Q1/68 , G01N27/447 , G01N27/26
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747 , Y10T436/143333
摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。
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公开(公告)号：US5091652A
公开(公告)日：1992-02-25
申请号：US531900
申请日：1990-06-01
申请人： Richard A. Mathies , Konan Peck
发明人： Richard A. Mathies , Konan Peck
CPC分类号： G02B21/0076 , G01N21/6458 , G01N27/44721 , G01N30/74 , G02B21/0028 , G02B21/004 , G01N2021/6439 , G01N2021/6476 , G01N21/6456 , G01N30/95
摘要： A fluorescent scanner for scanning the fluorescence from a fluorescence labeled separated sample on a sample carrier including a confocal microscope for illuminating a predetermined volume of the sample carrier and/or receiving and processing fluorescence emissions from said volume to provide a display of the separated sample.
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5.发明授权公开(公告)号：US06280933B1
公开(公告)日：2001-08-28
申请号：US08966398
申请日：1997-11-07
IPC分类号： C12Q168
CPC分类号： C07D417/14 , C07D221/12 , C07D405/12 , C07D417/06 , C09B23/04 , C12Q1/6816 , G01N27/44726 , G01N27/44747 , Y10T436/143333 , C12Q2565/102 , C12Q2563/173
摘要： Novel fluorescent labeling techniques and fluorescent labels are provided, employing high affinity non-covalently binding and intercalating fluorescent dyes and dsDNA. The dyes find application to provide highly sensitive labeling of nucleic acids in electrophoretic gels and as pre-prepared labels for binding to a wide variety of specific binding pair members. The DNA-dye fluorescer complex can be used for labels in diagnostic assays, detection of specific nucleic acid sequences, and the like.
摘要翻译： 提供新的荧光标记技术和荧光标记，采用高亲和力非共价结合和插入荧光染料和dsDNA。 染料可用于提供电泳凝胶中核酸的高灵敏度标记，以及预先制备的用于结合多种特异性结合对成员的标记。 DNA染料荧光剂复合物可用于诊断测定中的标记，特异性核酸序列的检测等。
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公开(公告)号：US20100252123A1
公开(公告)日：2010-10-07
申请号：US12819094
申请日：2010-06-18
CPC分类号： F16K99/0059 , B01L3/5025 , B01L3/502738 , B01L2200/12 , B01L2300/0816 , B01L2300/0887 , B01L2400/0638 , F16K99/0001 , F16K99/0015 , F16K99/0034 , F16K2099/0071 , F16K2099/0074 , F16K2099/0084 , Y10T137/2218 , Y10T137/3115 , Y10T137/5474 , Y10T137/87249
摘要： Membrane valves and latching valve structures for microfluidic devices are provided. A demultiplexer can be used to address the latching valve structures. The membrane valves and latching valve structures may be used to form pneumatic logic circuits, including processors.
摘要翻译： 提供了用于微流体装置的膜阀和闭锁阀结构。 解复用器可用于解决闭锁阀结构。 膜阀和闭锁阀结构可用于形成包括处理器在内的气动逻辑电路。
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公开(公告)号：US07766033B2
公开(公告)日：2010-08-03
申请号：US11726701
申请日：2007-03-21
IPC分类号： F16K31/128
CPC分类号： F16K99/0059 , B01L3/5025 , B01L3/502738 , B01L2200/12 , B01L2300/0816 , B01L2300/0887 , B01L2400/0638 , F16K99/0001 , F16K99/0015 , F16K99/0034 , F16K2099/0071 , F16K2099/0074 , F16K2099/0084 , Y10T137/2218 , Y10T137/3115 , Y10T137/5474 , Y10T137/87249
摘要： Membrane valves and latching valve structures for microfluidic devices are provided. A demultiplexer can be used to address the latching valve structures. The membrane valves and latching valve structures may be used to form pneumatic logic circuits, including processors.
摘要翻译： 提供了用于微流体装置的膜阀和闭锁阀结构。 解复用器可用于解决闭锁阀结构。 膜阀和闭锁阀结构可用于形成气动逻辑电路，包括处理器。
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8.发明授权Electrochemical detector integrated on microfabricated capilliary electrophoresis chips 失效电化学检测器集成在微细毛细管电泳芯片上
公开(公告)号：US6045676A
公开(公告)日：2000-04-04
申请号：US916557
申请日：1997-08-22
IPC分类号： G01N27/447 , G01N30/60 , G01N37/00 , G01N27/26
CPC分类号： G01N27/4473 , G01N27/44791 , G01N30/6095
摘要： A microfabricated capillary electrophoresis chip which includes an integral thin film electrochemical detector for detecting molecules separated in the capillary.
摘要翻译： 一种微细毛细管电泳芯片，其包括用于检测在毛细管中分离的分子的整体薄膜电化学检测器。
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公开(公告)号：US5436130A
公开(公告)日：1995-07-25
申请号：US143377
申请日：1993-10-26
IPC分类号： C12Q1/68 , G01N27/447 , C12P19/34 , G01N33/50
CPC分类号： C12Q1/6869 , G01N27/44721 , Y10T436/143333 , Y10T436/24
摘要： A DNA sequencing method described which uses single lane or channel electrophoresis. Sequencing fragments are separated in said lane and detected using a laser-excited, confocal fluorescence scanner. Each set of DNA sequencing fragments is separated in the same lane and then distinguished using a binary coding scheme employing only two different fluorescent labels. Also described is a method of using radio-isotope labels.
摘要翻译： 描述使用单泳道或通道电泳的DNA测序方法。 在所述泳道中分离测序片段并使用激光激发的共聚焦荧光扫描仪进行检测。 将每组DNA测序片段在相同的泳道中分离，然后使用仅使用两种不同荧光标记的二进制编码方案进行区分。 还描述了使用放射性同位素标记的方法。
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10.发明授权Inline-injection microdevice and microfabricated integrated DNA analysis system using same 有权在线注射微型装置和微制造综合DNA分析系统使用公开(公告)号：US08841116B2
公开(公告)日：2014-09-23
申请号：US11978224
申请日：2007-10-25
IPC分类号： C12M1/00 , C12M1/34 , C12M3/00 , G01N15/06 , B01D57/02 , C02F1/40 , B01L3/00 , G01N27/447 , G01N1/40 , B01L7/00
CPC分类号： G01N27/44743 , B01L3/502715 , B01L3/502753 , B01L3/502784 , B01L7/52 , B01L2200/027 , B01L2200/0631 , B01L2200/0673 , B01L2200/10 , B01L2300/0803 , B01L2400/0421 , B01L2400/0487 , G01N1/405
摘要： Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing.
摘要翻译： 提供用于毛细管电泳分析的用于在线注射核酸的方法和微流体电路。 根据各种实施方式，提供包括与分离通道一致的基于亲和性捕获矩阵的微制造结构。 基于亲和性的捕获矩阵提供在线样品塞形成和注入毛细管电泳通道。 还提供了用于DNA测序的基于微珠的在线注射系统的方法和装置。
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