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    EXPRESSION METHOD
    1.
    发明申请
    EXPRESSION METHOD 有权
    表达方法

    公开(公告)号：US20140220628A1

    公开(公告)日：2014-08-07

    申请号：US14110724

    申请日：2012-04-05

    申请人： Lukas Maksym ,  Ramona Knab ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

    发明人： Lukas Maksym ,  Ramona Knab ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

    IPC分类号： C12N15/87 ,  C12N9/54 ,  C12P21/00

    CPC分类号： C12N15/87 ,  C12N9/48 ,  C12N9/54 ,  C12N15/67 ,  C12P21/00 ,  C12P21/02

    摘要： The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.

    摘要翻译： 目的是增加微生物发酵中的蛋白质产物产量。 这通过引入微生物的方法来实现，不仅是编码蛋白质的第一表达构建体，而且还编码与蛋白质不同的辅助蛋白酶的第二表达构建体，其具有蛋白水解活性，并且其包含氨基酸序列， 与SEQ ID NO：1所示的氨基酸序列至少50％相同。 1。

    Expression Vector for an Improved Protein Secretion
    4.
    发明申请
    Expression Vector for an Improved Protein Secretion 有权
    用于改善蛋白质分泌的表达载体

    公开(公告)号：US20140356929A1

    公开(公告)日：2014-12-04

    申请号：US14122562

    申请日：2012-05-25

    申请人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

    发明人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

    IPC分类号： C12N9/50

    CPC分类号： C12N9/50 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75

    摘要： The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

    摘要翻译： 本发明的目的是改善蛋白质从宿主细胞的分泌，以增加发酵过程中蛋白质的产物产率。 这通过包含a）启动子序列和b）编码蛋白质的核酸序列的表达载体来实现。 该蛋白质包含信号肽和另外的氨基酸序列，并且信号肽包含与SEQ ID NO 2中指定的氨基酸序列至少80％相同的氨基酸序列，与氨基酸至少80％相同 SEQ ID NO 4中指定的序列与SEQ ID NO 6中指定的氨基酸序列至少80％相同，或者信号肽包含与至少一个所述序列结构同源的氨基酸序列。

    NUCLEIC ACID-BINDING CHIPS FOR DETECTING NITROGEN DEFICIENCIES AS PART OF BIOPROCESS CONTROL
    6.
    发明申请
    NUCLEIC ACID-BINDING CHIPS FOR DETECTING NITROGEN DEFICIENCIES AS PART OF BIOPROCESS CONTROL 审中-公开
    用于检测氮缺乏症的核酸结合剂作为生物控制的一部分

    公开(公告)号：US20100112552A1

    公开(公告)日：2010-05-06

    申请号：US11991761

    申请日：2006-09-07

    申请人： Stefan Evers ,  Jorg Feesche ,  Karl-Heinz Maurer ,  Thomas Schweder ,  Michael Hecker ,  Birgit Voigt ,  Britta Jurgen ,  Le Thi Hoi

    发明人： Stefan Evers ,  Jorg Feesche ,  Karl-Heinz Maurer ,  Thomas Schweder ,  Michael Hecker ,  Birgit Voigt ,  Britta Jurgen ,  Le Thi Hoi

    IPC分类号： C12Q1/68 ,  G01N33/00

    CPC分类号： C12Q1/689 ,  C12Q1/6837 ,  C12Q2600/158

    摘要： The invention relates to nucleic acid-binding chips for monitoring bioprocesses, specifically for detecting nitrogen deficiencies. Said chips carry probes that are sensitive to at least three of the following 50 genes: kdgR, citA, htrA, ycn1, yppF, trpB, ggt, alsR, glnA, nrgA, yciC, yvtA, nrgB, ycnJ, glnR, yvlA, yncE, yvlB, trpF, ydfS, trpD, ycnK, trpB, trpC, nasD, ycdH, nasC, nasB, trpE, pckA, nasF, yrkC, and tnrA or the homolgs to SEQ ID NO: 91, 41, 53, 19, 55, 47, 21, 17, 9, 85, 45, 49, 95, 63, 15, 93, or 81 at a maximum of 80 different probes that are specific of nitrogen metabolism. The invention also relates to the use of corresponding gene probes, especially on the aforementioned chips, to corresponding methods and possible uses.

    摘要翻译： 本发明涉及用于监测生物过程的核酸结合芯片，专门用于检测氮缺乏。 所述芯片携带对以下50个基因中的至少三个基因敏感的探针：kdgR，citA，htrA，ycn1，yppF，trpB，ggt，alsR，glnA，nrgA，yciC，yvtA，nrgB，ycnJ，glnR，yv1A，yncE ，yvlB，trpF，ydfS，trpD，ycnK，trpB，trpC，nasD，ycdH，nasC，nasB，trpE，pckA，nasF，yrkC和tnrA或与SEQ ID NO：91,41,53,19,55 ，47,21,17,9,55,45,49,95,63,15,133或81，最多80个不同的氮代谢特异性探针。 本发明还涉及相应的基因探针，特别是在上述芯片上的应用与相应的方法和可能的用途。