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公开(公告)号:US07040144B2
公开(公告)日:2006-05-09
申请号:US10207735
申请日:2002-07-26
申请人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill , Josh Molho , Anubhav Tripathi , Matthew B. Kerby
发明人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill , Josh Molho , Anubhav Tripathi , Matthew B. Kerby
CPC分类号: G01N27/44791 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L9/527 , B01L2200/027 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N11/08 , G01N2035/1044 , G05D7/0694
摘要: Microfluidic devices, systems, and methods measure viscosity, flow times, and/or pressures, other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.
摘要翻译: 微流体装置,系统和方法测量通道内的粘度,流动时间和/或压力,其它流动特性,并且所测量的流动特性可用于产生所需的流量。 多储层压力调节器和压力控制器系统,电动系统和/或其它流体输送机构可以产生流动,可控地混合流体等。
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公开(公告)号:US06681616B2
公开(公告)日:2004-01-27
申请号:US10008604
申请日:2001-11-09
申请人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
发明人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
IPC分类号: G01N1106
CPC分类号: G05D7/0694 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L9/527 , B01L2200/027 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N11/08 , G01N27/44791 , G01N2035/1044 , Y10T137/8158
摘要: Microfluidic devices, systems, and methods measure viscosity, flow times, and/or other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.
摘要翻译: 微流体装置,系统和方法测量通道内的粘度,流动时间和/或其他流动特性,并且可以使用测量的流动特性来产生期望的流量。 多储层压力调节器和压力控制器系统,电动系统和/或其它流体输送机构可以产生流动,可控地混合流体等。
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公开(公告)号:US06990851B2
公开(公告)日:2006-01-31
申请号:US10728528
申请日:2003-12-05
申请人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
发明人: Michael Spaid , Andrea W. Chow , Benjamin N. Wang , Ring-Ling Chien , J. Wallace Parce , Anne R. Kopf-Sill
CPC分类号: G05D7/0694 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L9/527 , B01L2200/027 , B01L2300/0663 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N11/08 , G01N27/44791 , G01N2035/1044 , Y10T137/8158
摘要: Microfluidic devices, systems, and methods measure viscosity, flow times, and/or other flow characteristics within the channels, and the measured flow characteristics can be used to generate a desired flow. Multi-reservoir pressure modulator and pressure controller systems, electrokinetic systems and/or other fluid transport mechanisms can generate the flow, controllably mix fluids, and the like.
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公开(公告)号:US06447661B1
公开(公告)日:2002-09-10
申请号:US09416288
申请日:1999-10-12
申请人: Andrea W. Chow , Robert S. Dubrow , J. Wallace Parce , Steven A. Sundberg , Jeffrey A. Wolk , Ring-Ling Chien , Steven James Gallagher , Michael R. Knapp , Anne R. Kopf-Sill , Tammy Burd Mehta
发明人: Andrea W. Chow , Robert S. Dubrow , J. Wallace Parce , Steven A. Sundberg , Jeffrey A. Wolk , Ring-Ling Chien , Steven James Gallagher , Michael R. Knapp , Anne R. Kopf-Sill , Tammy Burd Mehta
IPC分类号: G01N2726
CPC分类号: B01L3/502715 , B01L3/50273 , B01L3/502746 , B01L2200/027 , B01L2300/0816 , B01L2300/0838 , B01L2300/0867 , B01L2400/0406 , B01L2400/0415 , B01L2400/0487 , B01L2400/084 , G01N2035/00237 , G01N2035/1062 , Y10S366/02 , Y10T436/2575
摘要: Methods, apparatus and systems are provided for introducing large numbers of different materials into a microfluidic analytical device rapidly, efficiently and reproducibly. In particular, improved integrated pipettor chip configurations, e.g. sippers or electropipettors, are described which are capable of sampling extremely small amounts of material for which analysis is desired, transporting material into a microfluidic analytical channel network, and performing the desired analysis on the material.
摘要翻译: 提供了方法,装置和系统,用于将大量不同的材料快速,有效和可再现地引入微流体分析装置。 特别地,改进的集成移液器芯片配置,例如 描述了能够对需要分析的极少量材料进行取样,将材料输送到微流体分析通道网络中并对材料进行所需分析的能力。
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公开(公告)号:US06592821B1
公开(公告)日:2003-07-15
申请号:US09853887
申请日:2001-05-10
申请人: H. Garrett Wada , Anne R. Kopf-Sill , Marja Liisa Alajoki , J. Wallace Parce , Benjamin N. Wang , Andrea W. Chow , Robert S. Dubrow , Yevgeny Yurkovetsky , Javier Anibal Farinas
发明人: H. Garrett Wada , Anne R. Kopf-Sill , Marja Liisa Alajoki , J. Wallace Parce , Benjamin N. Wang , Andrea W. Chow , Robert S. Dubrow , Yevgeny Yurkovetsky , Javier Anibal Farinas
IPC分类号: G01N3348
CPC分类号: B01F13/0062 , B01J19/0093 , B01L3/502776 , B01L2200/0636 , B01L2400/0406 , B01L2400/0487 , G01N15/1404 , G01N27/44704 , G01N27/44791 , G01N2015/1409 , G01N2015/149 , G01N2333/4718 , Y10T436/11
摘要: Methods and systems for particle focusing to increase assay throughput and sensitivity in microscale systems are provided. The invention includes methods for providing substantially uniform flow velocity to flowing particles in microfluidic devices. Methods of sorting members of particle populations, such as cells and various subcellular components are also provided. Integrated systems in which particles are focused and/or sorted are additionally included.
摘要翻译: 提供了粒子聚焦的方法和系统,以增加微量体系中的分析产量和灵敏度。 本发明包括为微流体装置中的流动颗粒提供基本均匀的流速的方法。 还提供了分选颗粒群体成员的方法,例如细胞和各种亚细胞组分。 另外包括其中聚焦和/或分选颗粒的集成系统。
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公开(公告)号:US06506609B1
公开(公告)日:2003-01-14
申请号:US09569747
申请日:2000-05-11
申请人: H. Garrett Wada , Anne R. Kopf-Sill , Marja Liisa Alajoki , J. Wallace Parce , Benjamin N. Wang , Andrea W. Chow , Robert S. Dubrow
发明人: H. Garrett Wada , Anne R. Kopf-Sill , Marja Liisa Alajoki , J. Wallace Parce , Benjamin N. Wang , Andrea W. Chow , Robert S. Dubrow
IPC分类号: G01N700
CPC分类号: B01F13/0062 , B01J19/0093 , B01J2219/00889 , B01J2219/00891 , B01L3/502761 , B01L3/502776 , B01L2200/0636 , B01L2200/0652 , B01L2400/0406 , B01L2400/0487 , G01N15/1404 , G01N27/44704 , G01N27/44791 , G01N2015/1409 , G01N2015/149 , G01N2333/4718 , Y10T436/117497 , Y10T436/2575
摘要: Methods and systems for particle focusing to increase assay throughput in microscale systems are provided. The invention includes methods for providing substantially uniform flow velocity to flowing particles in microfluidic devices. Methods of sorting members of particle populations, such as cells and various subcellular components are also provided. Integrated systems in which particles are focused and/or sorted are additionally included.
摘要翻译: 提供了用于增加微尺度系统中测定通过量的粒子聚焦的方法和系统。 本发明包括为微流体装置中的流动颗粒提供基本均匀的流速的方法。 还提供了分选颗粒群体成员的方法,例如细胞和各种亚细胞组分。 另外包括其中聚焦和/或分选颗粒的集成系统。
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公开(公告)号:US20080131904A1
公开(公告)日:2008-06-05
申请号:US12017859
申请日:2008-01-22
申请人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
发明人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
摘要翻译: 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止,用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序,通过掺入荧光标记的核苷酸进行检测,任选地随后进行光漂白,并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件,包括粒子阵列,用于测序方法。
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公开(公告)号:US07105300B2
公开(公告)日:2006-09-12
申请号:US10413049
申请日:2003-04-14
申请人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
发明人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
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公开(公告)号:US06632655B1
公开(公告)日:2003-10-14
申请号:US09510626
申请日:2000-02-22
申请人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
发明人: Tammy Burd Mehta , Anne R. Kopf-Sill , J. Wallace Parce , Andrea W. Chow , Luc J. Bousse , Michael R. Knapp , Theo T. Nikiforov , Steve Gallagher
IPC分类号: C12M140
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Arrays of flowable or fixed particle sets are used in microfluidic systems for performing assays and modifying hydrodynamic flow. Also provided are assays utilizing flowable or fixed particle sets within a microfluidic system, as well as kits, apparatus and integrated systems comprising arrays and array members.
摘要翻译: 可流动或固定的颗粒组的数组用于微流体系统中,用于进行测定和改变流体动力学流动。 还提供了利用微流体系统内的可流动或固定的颗粒组的测定法,以及包括阵列和阵列成员的试剂盒,装置和集成系统。 PTEXT>
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公开(公告)号:US07566538B2
公开(公告)日:2009-07-28
申请号:US12017859
申请日:2008-01-22
申请人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
发明人: J. Wallace Parce , Theo T. Nikiforov , Tammy Burd Mehta , Anne R. Kopf-Sill , Andrea W. Chow , Michael R. Knapp
CPC分类号: C12Q1/6874 , B01F13/0059 , B01J2219/00317 , B01J2219/00459 , B01J2219/00468 , B01L3/5027 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C07H21/02 , C07H21/04 , C12Q1/6869 , C40B60/14 , G01N27/44756 , G01N35/0098 , G01N2035/00574 , Y10T436/143333 , C12Q2565/629 , C12Q2535/101 , C12Q2563/173 , C12Q2525/186 , C12Q2527/125
摘要: Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.
摘要翻译: 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止,用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序,通过掺入荧光标记的核苷酸进行检测,任选地随后进行光漂白,并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件,包括粒子阵列,用于测序方法。
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