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    Proteins encoded by ble genes and antibiotics from the bleomycin family
    4.
    发明申请
    Proteins encoded by ble genes and antibiotics from the bleomycin family 审中-公开
    来自博来霉素家族的可溶性基因和抗生素编码的蛋白质

    公开(公告)号：US20060194715A1

    公开(公告)日：2006-08-31

    申请号：US10532834

    申请日：2003-10-27

    申请人： Jonathan Blackburn ,  Darren Hart ,  Ben Godber ,  Mike McAndrews

    发明人： Jonathan Blackburn ,  Darren Hart ,  Ben Godber ,  Mike McAndrews

    IPC分类号： G01N33/554 ,  C07K9/00 ,  A61K38/14

    CPC分类号： G01N33/9446 ,  A61K47/6809 ,  C07K14/00 ,  G01N33/58 ,  G01N2415/00

    摘要： The present invention provides use of a protein conjugate comprising a “ble” protein, which has specific binding properties. The protein conjugates are capable of binding reversibly to an antibiotic from the bleomycin family, which property is exploited in a variety of immobilisation methods. In preferred aspects of the invention, the conjugates are used as markers for protein expression and/or folding, or for affinity tagging. The present invention also provides a probe comprising an array of an immobilised antibody from the bleomycin family, which acts as an analyte capture moiety. In another aspect, a purification media is provided, which comprises an antibiotic of the bleomycin family as an analyte capture moiety. Also provided is a method for generating soluble forms of an insoluble protein by expressing the protein as a “ble” fusion protein and selecting in the presence of an antibiotic from the bleomycin family. In a further aspect, the “ble” protein is expressed as a fusion protein in a cell into which is introduced a labelled antibiotic of the bleomycin family, thereby allowing identification of the cellular localisation of the protein.

    摘要翻译： 本发明提供了包含具有特异性结合特性的“可见”蛋白质的蛋白质缀合物的用途。 蛋白质缀合物能够可逆地结合来自博来霉素家族的抗生素，该特性在各种固定化方法中被利用。 在本发明的优选方面，缀合物用作蛋白质表达和/或折叠或亲和标记的标记物。 本发明还提供了包含来自博来霉素家族的固定化抗体阵列的探针，其作为分析物捕捉部分。 另一方面，提供了一种纯化介质，其包含作为分析物捕获部分的博来霉素家族的抗生素。 还提供了通过将蛋白质表达为“可见”融合蛋白并在来自博来霉素家族的抗生素存在下选择来产生可溶性形式的不溶性蛋白质的方法。 在另一方面，“可溶”蛋白质在细胞中表达为融合蛋白，其中引入了博来霉素家族的标记抗生素，从而允许鉴定蛋白质的细胞定位。

    Enzyme array and assay
    5.
    发明申请
    Enzyme array and assay 审中-公开
    酶阵列和测定

    公开(公告)号：US20060275855A1

    公开(公告)日：2006-12-07

    申请号：US10532787

    申请日：2003-10-27

    申请人： Jonathan Blackburn ,  Jens-Oliver Koopmann

    发明人： Jonathan Blackburn ,  Jens-Oliver Koopmann

    IPC分类号： C12Q1/48 ,  C12Q1/37 ,  C12Q1/26

    CPC分类号： C12Q1/00

    摘要： The present invention relates to an enzyme array and assay for use with a mass spectrometer, particularly, though not exclusively, a laser desorption/ionisation, such as a MALDI mass spectrometer. It includes a method of determining the activity of an enzyme, or the effect a test compound has on the activity of the enzyme, using mass spectrometry comprising: providing a probe carrying an immobilised enzyme; optionally introducing the test compound; introducing one or more reactants to the immobilised enzyme for a time, and in a form sufficient for a reaction to take place; drying the probe; subjecting the probe to mass spectrometry; and determining the activity of the enzyme, or the effect the test compound had on the activity of the enzyme, by detecting the presence and/or absence of one or more products and/or the one or more reactants and is exemplified with reference to a kinase assay. It also provides an array for use with the method.

    摘要翻译： 本发明涉及用于质谱仪的酶阵列和测定法，特别是但不限于激光解吸/电离，例如MALDI质谱仪。 其包括测定酶的活性的方法或测试化合物对酶的活性的影响，使用质谱法，其包括：提供携带固定化酶的探针; 任选地引入测试化合物; 将一种或多种反应物引入固定化酶一段时间，并以足以使反应发生的形式; 干燥探头; 对探针进行质谱分析; 并通过检测一种或多种产物和/或一种或多种反应物的存在和/或不存在来确定酶的活性或测试化合物对酶的活性的影响，并且参考 激酶测定。 它还提供了一个用于该方法的数组。

    Internal vent channel in ejection head assemblies and methods relating thereto
    6.
    发明申请
    Internal vent channel in ejection head assemblies and methods relating thereto 有权
    喷射头组件中的内部排气通道和与其相关的方法

    公开(公告)号：US20070206067A1

    公开(公告)日：2007-09-06

    申请号：US11365193

    申请日：2006-03-01

    申请人： Jonathan Blackburn ,  Edgar Diaz ,  Thomas Romine ,  Jeanne Singh ,  Mary Smoot ,  Jason Stokesbary

    发明人： Jonathan Blackburn ,  Edgar Diaz ,  Thomas Romine ,  Jeanne Singh ,  Mary Smoot ,  Jason Stokesbary

    IPC分类号： B41J2/175

    CPC分类号： B41J2/1753

    摘要： Fluid ejection head assemblies, fluid ejection devices, and methods for improving fluid sealing of fluid ejection head assemblies. One such fluid ejection head assembly includes a substrate cavity and a substantially planar surface surrounding the substrate cavity. The substantially planar surface contains at least one external vent, at least one internal vent channel, and a plurality of vents in fluid flow communication with the substrate cavity and providing fluid flow communication between the internal vent channel and the external vent. The plurality of vents, the at least one external vent and the at least one internal vent channel are disposed in fluid flow communication with an environment external to the substrate cavity for flow of a gas associated with an adhesive at least partially disposed in the substrate cavity, to the environment during the curing of the adhesive.

    摘要翻译： 流体喷射头组件，流体喷射装置和用于改善流体喷射头组件的流体密封的方法。 一个这样的流体喷射头组件包括衬底空腔和围绕衬底空腔的基本平坦的表面。 基本平坦的表面包含至少一个外部通气口，至少一个内部通气通道和与衬底空腔流体连通的多个通气口，并且在内部通气通道和外部通风口之间提供流体流动连通。 多个通气口，至少一个外部通气口和至少一个内部通气通道设置成与衬底空腔外部的环境流体流动连通，用于与至少部分地设置在衬底空腔中的粘合剂相关联的气体流动 ，在粘合剂固化期间的环境。

    Probe for mass spectrometry
    7.
    发明申请
    Probe for mass spectrometry 审中-公开
    探测质谱

    公开(公告)号：US20050196791A1

    公开(公告)日：2005-09-08

    申请号：US11054050

    申请日：2005-02-08

    申请人： Jens-Oliver Koopman ,  Jonathan Blackburn

    发明人： Jens-Oliver Koopman ,  Jonathan Blackburn

    IPC分类号： G01N27/64 ,  A61K47/48 ,  C07K14/00 ,  G01N27/62 ,  G01N33/483 ,  G01N33/53 ,  G01N33/94 ,  G01N37/00 ,  H01J49/04 ,  H01J49/16 ,  C12Q1/68 ,  C12M1/34 ,  H01J49/00

    CPC分类号： H01J49/0418 ,  A61K47/6809 ,  B82Y30/00 ,  C07K14/00 ,  G01N33/9446 ,  G01N2415/00 ,  Y10T436/24

    摘要： The present invention relates to a probe for the analysis of one or more analytes, particularly proteins or compounds capable of binding or otherwise interacting therewith, by laser desorption/ionisation mass spectrometry, more particularly MALDI MS. It also relates to a protein microarray, a method of producing a protein microarray and a method of analysing a protein microarray. The probe comprises a support having an electroconductive target surface thereon characterized in that the target surface comprises a micro array having a plurality of discrete target areas presenting one or more analyte capture moieties. Each discrete target area has an area of less than 1000 μm2, more preferably still less than 500 μm2, and more preferably still less than 100 μm2.

    摘要翻译： 本发明涉及通过激光解吸/电离质谱法，更具体地说是MALDI MS分析一种或多种分析物，特别是能够结合或以其它方式相互作用的蛋白质或化合物的探针。 它还涉及蛋白质微阵列，产生蛋白质微阵列的方法和分析蛋白质微阵列的方法。 探针包括其上具有导电目标表面的支撑体，其特征在于，目标表面包括具有呈现一个或多个分析物捕获部分的多个离散靶区域的微阵列。 每个离散目标区域的面积小于1000μm2，更优选小于500μm2，更优选小于100μm2， / SUP>。