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    Method for preferentially removing protein over nucleic acids using physical as well as chemical means of removal of the protein
    2.
    发明授权
    Method for preferentially removing protein over nucleic acids using physical as well as chemical means of removal of the protein 有权
    使用物理以及去除蛋白质的化学方法优先去除蛋白质的方法

    公开(公告)号：US07435811B2

    公开(公告)日：2008-10-14

    申请号：US11396761

    申请日：2006-04-03

    Applicant: Myo-yong Lee ,  Ki-woong Han

    Inventor： Myo-yong Lee ,  Ki-woong Han

    IPC: C07H21/00

    CPC classification number: C07K1/36 ,  C07K1/20 ,  C12Q1/6806 ,  C12Q2527/125

    Abstract: Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom or a substituted or unsubstituted C1-C6 alkyl group, a is an integer of 1 to 6, and b is 0 or 1, wherein b is 0 when a is not 1; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.

    Abstract translation: 提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中至少两个R R 1，R 2，R 3和R 3取代基是取代或未取代的C 1 -C 6烷基，而另一个取代基是氢原子或取代或未取代的 C1-C6烷基，a为1〜6的整数，b为0或1，a为1时，b为0; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。 通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。

    Microarray reaction device and method of using the same
    3.
    发明授权
    Microarray reaction device and method of using the same 失效
    微阵列反应装置及其使用方法

    公开(公告)号：US08647591B2

    公开(公告)日：2014-02-11

    申请号：US13007750

    申请日：2011-01-17

    Applicant: Won-seok Chung ,  Myo-yong Lee ,  Kak Namkoong ,  Woochang Lee

    Inventor： Won-seok Chung ,  Myo-yong Lee ,  Kak Namkoong ,  Woochang Lee

    IPC: B01L3/00 ,  B01L99/00 ,  F16K3/00 ,  C40B40/00 ,  C40B60/00 ,  F16K31/00

    CPC classification number: C40B30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/12

    Abstract: A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber.

    Abstract translation: 微阵列反应装置包括流体容器，反应室，与流体容器连接的第一通道，与反应室连接的第二通道和阀。 阀包括第一和第二支撑单元，分别包括延伸穿过其第一和第二表面的第一和第二穿透开口单元。 第一和第二穿透开口单元分别连接到第一和第二通道的第二端。 第二支撑单元包括延伸穿过其第二表面的第三穿透开口单元。 第一和第二表面彼此接触，使得第一支撑单元和第二支撑单元彼此可滑动地布置。 微阵列反应装置还包括与第三穿透开口单元连接的储存室，以及连接到储存室并向储存室提供压力的泵。

    Methods of isolating and amplifying nucleic acids using silanized solid support
    4.
    发明申请
    Methods of isolating and amplifying nucleic acids using silanized solid support 有权
    使用硅烷化固体支持物分离和扩增核酸的方法

    公开(公告)号：US20060264620A1

    公开(公告)日：2006-11-23

    申请号：US11345174

    申请日：2006-02-01

    Applicant: Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

    Inventor： Myo-yong Lee ,  Joong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Yeon-ja Cho ,  Shin-i Yoo

    IPC: C07H21/04

    CPC classification number: C07H21/04 ,  C12N15/1006

    Abstract: Provided are methods of isolating and amplifying nucleic acids from and in a nucleic acid-containing sample. The nucleic acid isolation method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support and treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14. The nucleic acid amplification method includes contacting a nucleic acid-containing sample to a silanized solid support to capture nucleic acids to the silanized solid support; treating the nucleic acid-captured solid support with an alkaline solution of pH 9 to 14; and adding a nucleic acid amplification solution to the resultant solution after the alkaline solution treatment to perform nucleic acid amplification.

    Abstract translation: 提供从含核酸样品中分离和扩增核酸的方法。 核酸分离方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化的固体支持物，并用pH9至14的碱性溶液处理核酸捕获的固体支持物。核酸扩增 方法包括将含核酸的样品与硅烷化的固体支持物接触以将核酸捕获到硅烷化固体支持物; 用pH9至14的碱性溶液处理核酸捕获的固体支持物; 并在碱溶液处理后向所得溶液中加入核酸扩增溶液进行核酸扩增。

    Nucleic acid purification method using hydrogen bonding and electric field
    6.
    发明申请
    Nucleic acid purification method using hydrogen bonding and electric field 审中-公开
    使用氢键和电场的核酸纯化方法

    公开(公告)号：US20060118417A1

    公开(公告)日：2006-06-08

    申请号：US11281226

    申请日：2005-11-16

    Applicant: Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

    Inventor： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

    IPC: C07H21/04

    CPC classification number: C07H21/04

    Abstract: Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.

    Abstract translation: 提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。 根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

    Apparatus for and method of purifying nucleic acids by different laser absorption of beads
    7.
    发明申请
    Apparatus for and method of purifying nucleic acids by different laser absorption of beads 审中-公开
    通过不同激光吸收珠粒来纯化核酸的装置和方法

    公开(公告)号：US20060110725A1

    公开(公告)日：2006-05-25

    申请号：US11190169

    申请日：2005-07-26

    Applicant: Jeong-gun Lee ,  Young-nam Kwon ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Young-a Kim

    Inventor： Jeong-gun Lee ,  Young-nam Kwon ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Young-a Kim

    IPC: C12Q1/70 ,  C12Q1/68 ,  C12N7/02 ,  C12N1/08 ,  C12M1/34

    CPC classification number: B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487

    Abstract: An apparatus for and method of purifying nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples, magnetic beads, and a solid support are introduced; a vibrator attached to the capillary and mixing the samples, magnetic beads, and solid support in the capillary; a laser generator attached to the capillary and irradiating a laser beam onto the capillary; a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall; a waste chamber attached to the capillary and discharging a lysate; an elution buffer chamber attached to the capillary and eluting nucleic acids from the solid support having nucleic acids bound thereto; and a neutralization buffer chamber attached to the capillary and supplying a neutralization buffer for neutralizing the eluted nucleic acid solution. According to the apparatus and method, PCR inhibitors can be removed to increase PCR yield and nucleic acids can be purified using a silicon substrate or silica beads. Thus, the apparatus and method can be applied to LOC fabrication.

    Abstract translation: 提供了一种纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品入口，通过所述样品入口引入样品，磁珠和固体支持物; 连接到毛细管并将样品，磁珠和固体支持物混合在毛细管中的振动器; 激光发生器附接到毛细管并将激光束照射到毛细管上; 附着到毛细管并将磁珠固定到毛细管壁的磁力发生器; 连接到毛细管并排出裂解物的废物室; 洗脱缓冲室连接到毛细管并从具有与其结合的核酸的固体支持物洗脱核酸; 以及中和缓冲室，其连接到毛细管并提供用于中和洗脱的核酸溶液的中和缓冲液。 根据该装置和方法，可以除去PCR抑制剂以增加PCR产量，并且可以使用硅底物或二氧化硅珠纯化核酸。 因此，该装置和方法可以应用于LOC制造。

    Method of purifying RNA using kosmotropic salt
    9.
    发明授权
    Method of purifying RNA using kosmotropic salt 有权
    使用去离子水纯化RNA的方法

    公开(公告)号：US07923551B2

    公开(公告)日：2011-04-12

    申请号：US12110391

    申请日：2008-04-28

    Applicant: Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

    Inventor： Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

    IPC: C07H21/00

    CPC classification number: C07H21/02 ,  C12N15/1003 ,  C12N15/1006

    Abstract: The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

    Abstract translation: 本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。 根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。 本发明在纯化200个或更少核苷酸的RNA中特别有效。

    Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser
    10.
    发明授权
    Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
    使用微磁珠和激光快速破坏细胞或病毒的方法和装置

    公开(公告)号：US07855069B2

    公开(公告)日：2010-12-21

    申请号：US11253541

    申请日：2005-10-19

    Applicant: Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

    Inventor： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

    IPC: C12M1/42 ,  C12M1/33

    CPC classification number: C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

    Abstract: A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

    Abstract translation: 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题 裂解芯片可应用于LOC。

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