公开(公告)号：US20050136464A1

公开(公告)日：2005-06-23

申请号：US11016588

申请日：2004-12-17

申请人： Peter Berndt ,  Stefan Evers ,  Hanno Langen

发明人： Peter Berndt ,  Stefan Evers ,  Hanno Langen

IPC分类号： G01N27/62 ,  C12Q1/06 ,  C12Q1/68 ,  G01N1/38 ,  G01N27/447 ,  G01N30/02 ,  G01N30/72 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/68 ,  G01N33/92 ,  G01N35/00

CPC分类号： G01N33/6803

摘要： The invention provides a method for the quantification of a biomolecule in a complex mixture of biomolecules which comprises a fractionation of the mixture of biomolecules providing at least two fractions with at least one distinct component each. These fractions are then subjected to serial combinatorial dilution. Subsequently, the biomolecule is detected and identified in the fractions by a method providing a sensitivity threshold and identify information. The quantity of the biomolecule is determined by summarizing the number of identifications of the biomolecule in each fraction on each dilution level in consideration of the respective dilution factor. For purpose of normalization this sum may be divided by the total number of identifications of all biomolecules in all fractions on all dilution levels.

摘要翻译： 本发明提供了一种在生物分子的复杂混合物中定量生物分子的方法，其包括生物分子的混合物的分馏，其提供至少两个级分和至少一种不同的组分。 然后对这些级分进行连续组合稀释。 随后，通过提供灵敏度阈值和识别信息的方法在分数中检测和鉴定生物分子。 考虑各自的稀释因子，通过总结每个稀释级别中每个级分中的生物分子的鉴定数来确定生物分子的量。 为了归一化，这个总和可以除以所有稀释水平上所有级分中所有生物分子的标识总数。

公开(公告)号：US09394528B2

公开(公告)日：2016-07-19

申请号：US11616319

申请日：2006-12-27

申请人： Cornelius Bessler ,  Jörg Feesche ,  Stefan Evers ,  Karl-Heinz Maurer ,  Armin Ehrenreich ,  Birgit Veith ,  Heiko Liesegang ,  Anke Henne ,  Christina Herzberg ,  Gerhard Gottschalk

发明人： Cornelius Bessler ,  Jörg Feesche ,  Stefan Evers ,  Karl-Heinz Maurer ,  Armin Ehrenreich ,  Birgit Veith ,  Heiko Liesegang ,  Anke Henne ,  Christina Herzberg ,  Gerhard Gottschalk

IPC分类号： C12N15/74 ,  C12N1/20 ,  C12P21/06 ,  C12N9/06 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/78 ,  C12N9/80 ,  C12N9/88 ,  C12N9/00 ,  C12N15/52 ,  C12P7/16 ,  C12P7/40 ,  C12P7/52 ,  C12P13/00

CPC分类号： C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1217 ,  C12N9/16 ,  C12N9/78 ,  C12N9/80 ,  C12N9/84 ,  C12N9/88 ,  C12N9/93 ,  C12N15/75 ,  C12P7/16 ,  C12P7/40 ,  C12P7/42 ,  C12P7/52 ,  C12P13/001 ,  C12Y101/01 ,  C12Y101/01035 ,  C12Y101/01157 ,  C12Y102/04002 ,  C12Y103/99 ,  C12Y203/01019 ,  C12Y206/01042 ,  C12Y207/02007 ,  C12Y305/01001 ,  C12Y305/01011 ,  C12Y401/01018 ,  C12Y401/01019 ,  C12Y402/01017 ,  C12Y402/01055 ,  C12Y602/01 ,  C12Y602/01001 ,  Y02E50/10 ,  Y02P20/52

摘要： The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived therefrom and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesized via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

摘要翻译： 本发明涉及地衣芽孢杆菌的25个迄今未描述的基因及其衍生的基因产物及其全部充分同源的核酸及其蛋白质。 它们发生在五种不同的代谢途径中，用于形成气味物质。 所讨论的代谢途径是用于合成：1）异戊酸（作为亮氨酸分解代谢的一部分），2）2-甲基丁酸和/或异丁酸（作为缬氨酸和/或异亮氨酸分解代谢的一部分） 3）丁醇和/或丁酸（作为丁酸代谢的一部分），4）丙酸（作为丙酸代谢的一部分）和/或5）尸胺和/或腐胺（作为赖氨酸分解代谢的一部分） 和/或精氨酸）。 这些基因的鉴定允许开发生物技术生产方法，其被改进到对于辅助这些核酸的程度，可以通过使微生物中相应的基因失活来减少通过这些代谢途径合成的恶臭物质的形成 用于生物技术生产。 此外，这些基因产物因此可用于制备反应或根据其各自生物化学性质的方法。

公开(公告)号：US07807443B2

公开(公告)日：2010-10-05

申请号：US11611945

申请日：2006-12-18

申请人： Jörg Feesche ,  Cornelius Bessler ,  Stefan Evers ,  Karl-Heinz Maurer ,  Armin Ehrenreich ,  Birgit Veith ,  Heiko Liesegang ,  Anke Henne ,  Christina Herzberg ,  Gerhard Gottschalk

发明人： Jörg Feesche ,  Cornelius Bessler ,  Stefan Evers ,  Karl-Heinz Maurer ,  Armin Ehrenreich ,  Birgit Veith ,  Heiko Liesegang ,  Anke Henne ,  Christina Herzberg ,  Gerhard Gottschalk

IPC分类号： C12N1/21 ,  C07H21/00 ,  C07K14/00 ,  C12N15/00 ,  C12N9/00 ,  C12P21/00

CPC分类号： C07K14/32 ,  C12N15/52 ,  C12P13/04

摘要： The invention relates to five or four novel genes and the gene products thereof from Bacillus licheniformis and sufficiently similar genes and proteins which are involved in vivo in the formation of polyamino acids. The gene in question is ywsC, ywsC′, ywtA, ywtB and ywtD or proteins coded thereby. The gene ywsC, ywsC′, ywtA and ywtB can be used to improve biotechnological production methods by microorganisms, wherein they are functionally inactivated; the gene ywtD which codes for a peptide decomposing poly-gamma glutamate can, inversely, contribute to the improvement of biotechnological production methods by increased expression. Said genes can be used positively, preferably to result in a modification or decomposition of poly-gamma glutamate.

摘要翻译： 本发明涉及5或4种新型基因及其从地衣芽孢杆菌中得到的基因产物以及与体内在聚氨基酸形成有关的充分相似的基因和蛋白质。 所讨论的基因是ywsC，ywsC'，ywtA，ywtB和ywtD或由此编码的蛋白质。 基因ywsC，ywsC'，ywtA和ywtB可用于通过微生物改善生物技术生产方法，其中它们在功能上失活; 编码分解聚-γ谷氨酸的肽的基因ywtD可以相反地通过增加表达来促进生物技术生产方法的改进。 所述基因可以积极使用，优选导致聚-γ谷氨酸的修饰或分解。

公开(公告)号：US09803183B2

公开(公告)日：2017-10-31

申请号：US14122562

申请日：2012-05-25

申请人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

发明人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

IPC分类号： A61K38/00 ,  C12N9/50 ,  C12N15/62 ,  C12N15/70 ,  C12N15/75

CPC分类号： C12N9/50 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75

摘要： The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a promoter sequence and a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

公开(公告)号：US09663798B2

公开(公告)日：2017-05-30

申请号：US14110724

申请日：2012-04-05

申请人： Lukas Maksym ,  Ramona Knab ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

发明人： Lukas Maksym ,  Ramona Knab ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

IPC分类号： C12N15/87 ,  C12P21/00 ,  C12N9/48 ,  C12N15/67 ,  C12P21/02 ,  C12N9/54

CPC分类号： C12N15/87 ,  C12N9/48 ,  C12N9/54 ,  C12N15/67 ,  C12P21/00 ,  C12P21/02

摘要： The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.

公开(公告)号：US20100009462A1

公开(公告)日：2010-01-14

申请号：US11658517

申请日：2005-07-19

申请人： Andreas Christ ,  Stefan Evers ,  Kurt Krapfenbauer ,  Elena Sebokova

发明人： Andreas Christ ,  Stefan Evers ,  Kurt Krapfenbauer ,  Elena Sebokova

IPC分类号： G01N33/566 ,  G01N33/68

CPC分类号： G01N33/6893 ,  G01N2333/8146 ,  G01N2500/04 ,  G01N2800/042 ,  G01N2800/52

摘要： The present invention relates to the monitoring of disease progression and diagnosis of beta-cell failure in diabetes by measuring levels of TIMP-2 in a liquid sample, and to screening for novel compounds for the prevention and/or treatment of diabetes.

摘要翻译： 本发明涉及通过测量液体样品中TIMP-2的水平以及筛选用于预防和/或治疗糖尿病的新化合物来监测糖尿病中疾病进展和β细胞衰竭的诊断。

公开(公告)号：US06569622B1

公开(公告)日：2003-05-27

申请号：US09291046

申请日：1999-04-14

申请人： Michel Arthur ,  Sylvie Dutka-Malen ,  Stefan Evers ,  Patrice Courvalin

发明人： Michel Arthur ,  Sylvie Dutka-Malen ,  Stefan Evers ,  Patrice Courvalin

IPC分类号： C12Q168

CPC分类号： C12N9/93 ,  C07K14/315

摘要： The invention relates to a protein VanB involved, in Gram-positive bacteria, in resistance to glycopeptides, particularly to vancomycine, said resistance being of the type inducible by the vancomycine and non-inducible by teicoplanine. The invention also relates to the utilisation of fragments of nucleotides of the gene van B for the detection of resistances to glycopeptides.

摘要翻译： 本发明涉及在革兰氏阳性菌中涉及对糖肽，特别是对万古霉素的抗性的蛋白质VanB，所述抗性是由万古霉素诱导的并且不被泰可菌素诱导的类型。 本发明还涉及使用基因van B的核苷酸片段来检测对糖肽的抗性。

公开(公告)号：US20140356929A1

公开(公告)日：2014-12-04

申请号：US14122562

申请日：2012-05-25

申请人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

发明人： Christian Degering ,  Thorsten Eggert ,  Stefan Evers ,  Karl-Heinz Maurer ,  Johannes Bongaerts

IPC分类号： C12N9/50

CPC分类号： C12N9/50 ,  C12N15/625 ,  C12N15/70 ,  C12N15/75

摘要： The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

摘要翻译： 本发明的目的是改善蛋白质从宿主细胞的分泌，以增加发酵过程中蛋白质的产物产率。 这通过包含a）启动子序列和b）编码蛋白质的核酸序列的表达载体来实现。 该蛋白质包含信号肽和另外的氨基酸序列，并且信号肽包含与SEQ ID NO 2中指定的氨基酸序列至少80％相同的氨基酸序列，与氨基酸至少80％相同 SEQ ID NO 4中指定的序列与SEQ ID NO 6中指定的氨基酸序列至少80％相同，或者信号肽包含与至少一个所述序列结构同源的氨基酸序列。

公开(公告)号：US20110201536A1

公开(公告)日：2011-08-18

申请号：US13026491

申请日：2011-02-14

申请人： Timothy O'Connell ,  Petra Siegert ,  Stefan Evers ,  Johannes Bongaerts ,  Thomas Weber ,  Karl-Heinz Maurer ,  Cornelius Bessler

发明人： Timothy O'Connell ,  Petra Siegert ,  Stefan Evers ,  Johannes Bongaerts ,  Thomas Weber ,  Karl-Heinz Maurer ,  Cornelius Bessler

IPC分类号： C11D3/60

CPC分类号： C11D3/3869 ,  C11D1/32 ,  C11D3/2003 ,  C11D3/201 ,  C11D3/2041 ,  C11D3/2044 ,  C11D3/2065 ,  C11D3/2072 ,  C11D3/2075 ,  C11D3/2079 ,  C11D3/2082 ,  C11D3/2086 ,  C11D3/221 ,  C11D3/222 ,  C11D3/33 ,  C11D3/3719 ,  C11D3/3723 ,  C11D3/3753 ,  C11D3/3757 ,  C11D3/3769 ,  C11D3/381 ,  C11D3/386 ,  C11D3/38627 ,  C11D3/38636 ,  C11D3/38645

摘要： The present invention relates to a method for increasing the cleaning performance of a washing or cleaning agent comprising at least one hydrolytic enzyme by addition of a component capable of synergistic interaction with the enzyme. Components capable of such positive synergistic interaction with hydrolytic enzymes include: (i) an amino acid, a polyamino acid, or derivatives thereof; (ii) a biosurfactant; (iii) a microbial metabolite, and a preparation of a microbial culture supernatant that contains at least 2.5 wt % of (i), (ii), or (iii). Additionally, the present invention comprises a method for washing textiles or hard surfaces with an enzymatic washing or cleaning agent that includes at least one of the components (i), (ii), or (iii) capable of increasing the cleaning performance of the washing or cleaning agent through synergistic interaction with the hydrolytic enzyme.

摘要翻译： 本发明涉及通过添加能够与酶协同相互作用的成分来提高包含至少一种水解酶的洗涤剂或清洁剂的清洗性能的方法。 能够与水解酶具有这种正相互作用的组分包括：（i）氨基酸，聚氨基酸或其衍生物; （ii）生物表面活性剂; （iii）微生物代谢物和含有至少2.5wt％（i），（ii）或（iii）的微生物培养物上清液的制备。 此外，本发明包括用酶洗涤剂或清洁剂洗涤纺织品或硬表面的方法，所述方法包括能够增加洗涤物的清洁性能的组分（i），（ii）或（iii）中的至少一种 或通过与水解酶的协同相互作用的清洁剂。

公开(公告)号：US20090215069A1

公开(公告)日：2009-08-27

申请号：US11658395

申请日：2005-07-19

申请人： Andreas Christ ,  Stefan Evers ,  Kurt Krapfenbauer ,  Elena Sebokova

发明人： Andreas Christ ,  Stefan Evers ,  Kurt Krapfenbauer ,  Elena Sebokova

IPC分类号： G01N33/53

CPC分类号： G01N33/6893 ,  G01N2333/575 ,  G01N2500/04 ,  G01N2800/042 ,  G01N2800/52

摘要： The present invention relates to the monitoring of disease progression and diagnosis of beta-cell failure in diabetes by measuring levels of pancreatic hormone in a liquid sample, and to screening for novel compounds for the prevention and/or treatment of diabetes.

摘要翻译： 本发明涉及通过测量液体样品中胰激素的水平以及筛选用于预防和/或治疗糖尿病的新化合物来监测糖尿病中的β-细胞衰竭的疾病进展和诊断。