摘要:
The invention provides seminal computational approaches utilizing data from non-rare cells to detect rare cells, such as circulating tumor cells (CTCs). The invention is applicable at two distinct stages of CTC detection; the first being to make decisions about data collection parameters and the second being to make decisions during data reduction and analysis. Additionally, the invention utilizes both one and multi-dimensional parameterized data in a decision making process.
摘要:
The present application provides methods for obtaining single cells from a sample. Methods for isolating and analyzing molecular features obtained from a single cell are also disclosed herein. For example, individual circulating tumor cells (CTCs) from a sample such as a patient's blood sample can be identified and obtained using methods disclosed herein, and picked for further analysis.
摘要:
A diaphragm air pump comprises a pump chamber, a diaphragm and one or more piezoelectric beams or bimorphs. Fluid flows into the pump chamber and then flows out of it, the diaphragm is provided within the pump chamber, and one or more central openings are formed in the diaphragm. One or more central check valves are provided in the central openings. The diaphragm is just bonded with piezoelectric beams, not fixed to the lower housing of the pump chamber in order to get large displacement. With the provided diaphragm air pump, it is possible to actively adjust the air quantity according to the requirement for fuel cell or a part-to-be-cooled, and it is also possible to reduce noise and power consumption compared with a conventional fan type cooler or air pumps.
摘要:
The present disclosure relates to methods of quantifying nucleic acid molecules immobilized on an electrode of an electrochemical assay chip. Such quantification can be used in testing and quality control of assay chips. Materials and instrumentation useful in performing such techniques are also disclosed.
摘要:
An anti stiction structure for cantilever formation technique. In one embodiment, the polymer cantilever is prevented from sticking to the substrate by at amortized stick layer on the substrate during formation that is later removed as a sacrificial layer. In another embodiment, the cantilever includes downwardly extending legs.
摘要:
A micromachined fluid handling device having improved properties. The valve is made of reinforced parylene. A heater heats a fluid to expand the fluid. The heater is formed on unsupported silicon nitride to reduce the power. The device can be used to form a valve or a pump. Another embodiment forms a composite silicone/parylene membrane. Another feature uses a valve seat that has concentric grooves for better sealing operation.
摘要:
The present disclosure provides methods and apparatuses of a highly sensitive and reliable IoT location tracking device in a form factor of a vehicle taillight. The tracking device replaces the existing taillight and comprises custom-designed GPS and Cellular antennae that are specially integrated with a novel dual-PCBA structure and an anti-theft tactile switch detection mechanism. The purposes of the new methods and apparatuses are to achieve the following: (1) improved reliability of signal reception and transmission with taillight integration; (2) improved electronic shielding of the processor board and PCB (printed circuit board) assembly; (3) high sensitivity of the new GPS and cellular antennae geometry and dimension design; (4) novel anti-theft security and better vehicle tracking management.
摘要:
A system including a plurality of coil elements is provided. Each coil element is arranged with a first switch and a second switch. In a first mode, the first switch and the second switch are turned off to split each coil element into a first coil portion and a second coil portion, to transmit first radio frequency signals. In a second mode, the first switch and second switch are turned on to transform each coil element into a loop coil to simultaneously transmit or receive multiple second radio frequency signals.
摘要:
Methods for conducting an assay to detect nucleic acid hybridization are disclosed. In particular, the fabrication of a carbon electrode suited to nucleic acid hybridization detection is described. In some preferred embodiments, a micro array of carbon electrodes is constructed using photolithography. The final step in the photolithography process involves developing a solder mask with an alkaline solution. The alkaline solution oxidizes the carbon surface producing surface carboxylic acid groups. The surface carboxylic acid groups are reacted with EDC or DCC and NHS to produce NHS esters. Immobilization of NEUTRAVIDIN® onto the electrode surface is effected by reaction with the surface NHS ester groups. A biotinylated probe DNA molecule is then attached to the electrode via binding between the biotin group and the immobilized NEUTRAVIDIN®. The resulting electrode can be used to detect hybridization between the probe DNA and complementary target DNA.