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    Enzymatic conversion of .alpha.-hydroxynitriles to the corresponding .alpha .
    2.
    发明授权
    Enzymatic conversion of .alpha.-hydroxynitriles to the corresponding .alpha . 失效
    α-羟基腈向相应的α-羟基酰胺，酸或酸式盐的酶转化

    公开(公告)号：US5866379A

    公开(公告)日：1999-02-02

    申请号：US790675

    申请日：1997-01-28

    申请人： Richard P. Burlingame ,  James R. Millis ,  Fernando Sanchez-Riera ,  Thomas F. Blackburn ,  Alan D. Grund

    发明人： Richard P. Burlingame ,  James R. Millis ,  Fernando Sanchez-Riera ,  Thomas F. Blackburn ,  Alan D. Grund

    IPC分类号： C12P7/42 ,  C12P11/00 ,  C12P13/02

    CPC分类号： C12R1/01 ,  C12P11/00 ,  C12P13/02 ,  C12P7/42 ,  Y10S435/822

    摘要： Enzymes derived from the isolated and substantially purified microorganisms of the present invention, designated herein as strains 52 and 56wt, are capable of hydrating nitriles such as 2-hydroxy-4-(methylthio)-butanenitrile (HMB-nitrile) to their corresponding amides, and further, of hydrolyzing amides such as 2-hydroxy-4-(methylthio)-butaneamide (HMB-amide) to their corresponding carboxylic acids. Advantageously, the nitrile hydratase of these strains is not substantially inhibited by the .alpha.-hydroxybutyramide product being formed; rather, this enzyme maintains the ability to hydrate an .alpha.-hydroxybutyronitrile to its corresponding amide even at high amide concentrations, including at saturating amide conditions. As such, enzymes derived from strains 52 and 56wt are particularly suited for commercial use in preparing agrichemical intermediates such as HMB-amide. HMB-amide may, in turn, be hydrolyzed through traditional or enzymatic conversion methods to form HMB-acid or salts thereof, which are useful as methionine substitutes in feeds for domestic animals.

    摘要翻译： 衍生自本发明的分离和基本纯化的微生物的酶，其在本文中称为菌株52和56wt，能够将腈如2-羟基-4-（甲硫基）丁腈（HMB-腈）水合至其相应的酰胺， 此外，将酰胺如2-羟基-4-（甲硫基） - 丁酰胺（HMB-酰胺）水解成其相应的羧酸。 有利地，这些菌株的腈水合酶基本上不被形成的α-羟基丁酰胺产物抑制; 相反，该酶保持即使在高酰胺浓度（包括在饱和酰胺条件下）将α-羟基丁腈水解成其相应的酰胺的能力。 因此，源自菌株52和56wt的酶特别适用于制备农药中间体如HMB-酰胺的商业用途。 HMB-酰胺又可以通过传统的或酶促转化方法水解形成HMB-酸或其盐，它们可用作家畜饲料中的甲硫氨酸替代物。

    PRODUCTION OF COMMERCIAL BIODIESEL FROM GENETICALLY MODIFIED MICROORGANISMS
    7.
    发明申请
    PRODUCTION OF COMMERCIAL BIODIESEL FROM GENETICALLY MODIFIED MICROORGANISMS 审中-公开
    从遗传改良的微生物生产商业生物系统

    公开(公告)号：US20100257777A1

    公开(公告)日：2010-10-14

    申请号：US12758000

    申请日：2010-04-11

    申请人： Fernando Sanchez-Riera ,  Wei Huang ,  Vineet Shastry

    发明人： Fernando Sanchez-Riera ,  Wei Huang ,  Vineet Shastry

    IPC分类号： C10L1/19

    CPC分类号： C10L1/026 ,  C10L2200/0476 ,  C10L2270/026 ,  C10L2290/26 ,  C12P7/649 ,  Y02E50/13 ,  Y02P30/20

    摘要： The invention provides a fermentation and recovery process for the production of biodiesel of commercial grade quality according to commercial and environmental standards (e.g., ASTM ANP, or EPA trace elements and emissions standards), by fermentation of carbohydrates using a genetically modified microorganism. The process provides a direct route for the production of fatty esters, without the need for producing oils which are later chemically transesterified with the concomitant production of large quantities of glycerin and other undesirable side-products.

    摘要翻译： 本发明通过使用遗传修饰的微生物发酵碳水化合物，根据商业和环境标准（例如，ASTM ANP或EPA微量元素和排放标准）提供用于生产商业级质量生物柴油的发​​酵和回收方法。 该方法提供了用于生产脂肪酯的直接途径，而不需要生产随后通过大量甘油和其它不期望的副产物的伴随产生化学酯交换的油。

    Methods And Systems For Increasing Production of Equilibrium Reactions
    8.
    发明申请
    Methods And Systems For Increasing Production of Equilibrium Reactions 失效
    增加平衡反应生产的方法和系统

    公开(公告)号：US20080015361A1

    公开(公告)日：2008-01-17

    申请号：US11752492

    申请日：2007-05-23

    申请人： Anil Khare ,  Brent Hilbert ,  Christopher Solheid ,  Sara McFarlan ,  Fernando Sanchez-Riera ,  Kent Floy ,  Paula Hicks

    发明人： Anil Khare ,  Brent Hilbert ,  Christopher Solheid ,  Sara McFarlan ,  Fernando Sanchez-Riera ,  Kent Floy ,  Paula Hicks

    IPC分类号： C07D209/04 ,  B01J8/00

    CPC分类号： C07D209/20 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3804 ,  C12P17/10

    摘要： Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

    摘要翻译： 描述了用于增加平衡反应生成的方法和系统。 在一些实施方案中，一种方法包括一旦达到平衡即从反应混合物中除去酶，选择性地重新加入用于将产物形成反应驱动到产物中的酶和反应物，以及任选地再循环稳定的中间体，副产物和/或副产物 回到反应混合物中。 在一些实施方案中，方法包括从反应混合物中纯化所需产物，并根据需要选择性地将反应混合物中的一种或多种组分（例如原始反应物，中间体，副产物或副产物）循环回到反应混合物中，以改善 滴度。 还提供了实施方法的系统。 在一些实施方案中，实施方法和系统用于增加在多步平衡途径中产生的莫纳甜和莫纳甜衍生物的产生。

    Production of GGL with low environmental impact
    9.
    发明申请
    Production of GGL with low environmental impact 有权
    GGL生产环境影响低

    公开(公告)号：US20060134742A1

    公开(公告)日：2006-06-22

    申请号：US11015563

    申请日：2004-12-17

    申请人： Brian Brazeau ,  Jean-Claude De Troostembergh ,  Paula Hicks ,  Holly Jessen ,  Sara McFarlan ,  Willy Obyn ,  Fernando Sanchez-Riera ,  William Schroeder

    发明人： Brian Brazeau ,  Jean-Claude De Troostembergh ,  Paula Hicks ,  Holly Jessen ,  Sara McFarlan ,  Willy Obyn ,  Fernando Sanchez-Riera ,  William Schroeder

    IPC分类号： C12P21/06 ,  C12N9/02 ,  C12N1/21

    CPC分类号： C12P7/58 ,  C12P17/181 ,  C12P19/02

    摘要： A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono-γ-lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono-γ-lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.

    摘要翻译： 公开了一种增加肌醇加氧酶比活性的方法。 该方法包括在有效提高肌醇加氧酶比活性的条件下，将包含肌醇加氧酶和不含硫的还原剂的混合物进行温育。 还公开了生产D-葡萄糖醛酸和葡糖苷酸-γ-内酯的方法，包括在有效地将每升混合物形成5克D-葡萄糖醛酸的条件下孵育包括肌醇，肌醇加氧酶和氧气的混合物至400 克D-葡萄糖醛酸每升混合物。 葡糖醛酸-γ-内酯可以由D-葡萄糖醛酸产物制备。 还公开了适用于这种方法的生物体和核酸。