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    Composition comprising various proteorhodopsins and/or bacteriorhodopsins and use thereof
    4.
    发明授权
    Composition comprising various proteorhodopsins and/or bacteriorhodopsins and use thereof 有权
    包含各种蛋白质视紫红质和/或细菌视紫红质的组合物及其用途

    公开(公告)号：US07982000B2

    公开(公告)日：2011-07-19

    申请号：US11569862

    申请日：2005-06-09

    申请人： Richard R. Bott ,  Rasmus B. Jensen ,  Bradley R. Kelemen ,  Donald E. Ward, II ,  Gregory M. Whited

    发明人： Richard R. Bott ,  Rasmus B. Jensen ,  Bradley R. Kelemen ,  Donald E. Ward, II ,  Gregory M. Whited

    IPC分类号： C07K17/00 ,  G11B11/00 ,  G11B7/085 ,  G11B7/00 ,  G11B5/84 ,  G11B7/24 ,  B32B3/02 ,  G03F7/00 ,  G02F1/09

    CPC分类号： G11C7/00 ,  C07K14/215 ,  C09D11/037 ,  C09D11/50 ,  G11B7/242 ,  Y10S430/146 ,  Y10T428/249921 ,  Y10T428/31623 ,  Y10T428/31685 ,  Y10T428/31725 ,  Y10T428/31783

    摘要： The present invention provides a solid material comprising an immobilized mixture of two or more proteorhodopsins, two or more bacteriorhodopsins, or one or more bacteriorhodopsin and one or more proteorhodopsins. The proteorhodopsins are selected from the group consisting of all-trans-retinal-containing proteorhodopsins and retinal analog-containing proteorhodopsins; all of which have absorption spectra that do not overlap. The bacteriorhodopsins are selected from the group consisting of all-trans-retinal-containing bacteriorhodopsins and retinal analog-containing bacteriorhodopsins; all of which have absorption spectra that do not overlap. The present invention also provides an optical information carrier, such as an optical data storage material and a fraud-proof optical data carrier, comprising the above-described solid material and a substrate selected from the group consisting of glass, paper, metal, fabric material, and plastic material, wherein said solid material is deposited on said substrate. The present invention further provides security ink comprising one or more hydrophilic polymers and a mixture of various photochromic materials.

    摘要翻译： 本发明提供了一种固体材料，其包含两种或多种蛋白质视紫红质，两种或更多种细菌视紫红质，或一种或多种细菌视紫红质和一种或多种蛋白质视紫质的固定化混合物。 蛋白质视紫红质选自全反式视黄醛的蛋白质视黄素和含视网膜类似物的蛋白质视紫红质; 所有这些都具有不重叠的吸收光谱。 细菌视紫红质选自全反式视网膜含细菌视紫红质和视网膜类似物的细菌视紫红质; 所有这些都具有不重叠的吸收光谱。 本发明还提供一种光学信息载体，例如光学数据存储材料和防欺诈光学数据载体，其包括上述固体材料和选自玻璃，纸，金属，织物材料的基材 和塑料材料，其中所述固体材料沉积在所述基底上。 本发明还提供了包含一种或多种亲水聚合物和各种光致变色材料的混合物的安全油墨。

    Subtilisin variants
    7.
    发明授权
    Subtilisin variants 失效
    枯草杆菌蛋白酶变体

    公开(公告)号：US07250281B2

    公开(公告)日：2007-07-31

    申请号：US10323324

    申请日：2002-12-18

    申请人： Thomas P. Graycar ,  Richard R. Bott ,  Lori J. Wilson

    发明人： Thomas P. Graycar ,  Richard R. Bott ,  Lori J. Wilson

    IPC分类号： C12N9/56 ,  C12N15/57 ,  C12D15/74 ,  C11D3/386

    CPC分类号： C11D3/38609 ,  C12N9/54

    摘要： Novel carbonyl hydrolase variants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The variant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such variant carbonyl hydrolases have properties which are different from those of the precursor hydrolase, such as altered proteolytic activity, altered stability, etc. The substituted amino acid residues correspond to positions +76 in combination with one or more of the following residues +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265 and/or +274 in Bacillus amyloliquefaciens subtilisin.

    摘要翻译： 公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶变体。 变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体羰基水解酶具有与前体水解酶不同的性质，例如改变的蛋白水解活性，改变的稳定性等。取代的氨基酸残基对应于与一个或多个以下残基组合的位置+76， +101，+103，+104，+107，+123，+27，+105，+ 109，+126，+128，+135，+ 156，+166，+ 195，+ 197，+ 204，+ 206 ，+210，+ 216，+ 217，+ 218，+ 222，+ 260，+ 265和/或+ 274的解淀粉芽孢杆菌枯草杆菌蛋白酶。

    Subtilisin modifications to enhance oxidative stability
    9.
    发明授权
    Subtilisin modifications to enhance oxidative stability 失效
    枯草杆菌蛋白酶修饰以增强氧化稳定性

    公开(公告)号：US5346823A

    公开(公告)日：1994-09-13

    申请号：US36592

    申请日：1993-03-24

    申请人： David A. Estell ,  James A. Wells ,  Richard R. Bott

    发明人： David A. Estell ,  James A. Wells ,  Richard R. Bott

    IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N15/10 ,  C12N15/75 ,  C12N15/00 ,  C12N15/11 ,  C12N15/57

    CPC分类号： C12R1/07 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54

    摘要： There are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.

    摘要翻译： 提供了制备具有改变的氧化稳定性的突变型枯草杆菌蛋白酶的方法，所述方法包括获得由编码枯草杆菌蛋白酶的区域组成的DNA片段，并将突变引入所述DNA片段，使得突变引入到编码 敏感的蛋氨酸，色氨酸，半胱氨酸或赖氨酸，使得突变型枯草杆菌蛋白酶表达后，编码甲硫氨酸，色氨酸，半胱氨酸或赖氨酸的一个或多个密码子区域被甲硫氨酸，色氨酸，半胱氨酸或赖氨酸以外的氨基酸替代， 优选丙氨酸或丝氨酸。