摘要:
A method is provided for forming crystallization experiments for a molecule, the method comprising forming a plurality of crystallization experiments that comprise a molecule to be crystallized and a composition that varies among the plurality of crystallization experiments, the crystallization experiments having a volume less than 1 microliter, the volumes of the plurality of crystallization experiments varying among the experiments by less than 25 nL.
摘要:
A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.
摘要:
A method is provided for performing crystallization experiments for a molecule, the method comprising performing a plurality of crystallization experiments where the crystallization experiments have volumes of less than 1 microliter, the crystallization experiments comprising a molecule to be crystallized and a composition that varies among the plurality of crystallization experiments detecting crystal formation in the crystallization experiments.
摘要:
A system for locking a door can: (1) lock the door in dead bolt fashion, (2) allow the door to open a limited distance, or (3) allow the door to open freely. The system has a door-mounted latch assembly that includes a base plate, an intermediate link pivoted to the base plate, and a mode selection and latching assembly that is pivoted to the intermediate link. The latch assembly also includes a high strength, "T"-shaped latch member, and camming means to slide the latch member back and forth as a control knob is rotated. A latch plate is mounted on the door frame in juxtaposition to the door-mounted latch assembly. The latch plate has a cylindrical portion with a vertical slot for receiving the "T"-shaped latch member. A cylindrical sleeve fits inside the cylindrical portion of the latch plate. Like the cylindrical portion of the latch plate, the sleeve has a vertical slot for receiving the latch member. The sleeve also has a horizontal slot running perpendicular to and intersecting with the vertical slot. When the latch member is engaged in the cylindrical portion of the latch plate, the cylindrical sleeve can be rotated so that the latch member is engaged with the horizontal slot in the cylindrical sleeve. The latch member then cannot be directly withdrawn from the latch plate.
摘要:
A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.
摘要:
A method and apparatus for the transportation, remote and unattended mounting, and visual alignment and monitoring of protein crystals for synchrotron generated x-ray diffraction analysis. The protein samples are maintained at liquid nitrogen temperatures at all times: during shipment, before mounting, mounting, alignment, data acquisition and following removal. The samples must additionally be stably aligned to within a few microns at a point in space. The ability to accurately perform these tasks remotely and automatically leads to a significant increase in sample throughput and reliability for high-volume protein characterization efforts. Since the protein samples are placed in a shipping-compatible layered stack of sample cassettes each holding many samples, a large number of samples can be shipped in a single cryogenic shipping container.
摘要:
A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.
摘要:
A portable, fold-up sports chair includes fabric seat and back portions, attached to and extending between a lightweight, collapsible tubular frame. The transverse frame members are hinged to allow the chair to be folded into a compact bundle for transportation or storage. Straps attached to the underside of the legs help prevent the chair legs from penetrating or sinking when used on a soft surface such as sand. The angle of the seat back is adjustable by means of straps which also serve as arm rests when the chair is in use.
摘要:
A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of E. coli proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.
摘要:
A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule, the method comprising: forming an array of microcrystallizations, each microcrystallization comprising a drop comprising a mother liquor solution whose composition varies within the array and a molecule to be crystallized, the drop having a volume of less than 1 microliter; storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops by taking images of the drops.