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公开(公告)号：US6074827A
公开(公告)日：2000-06-13
申请号：US18918
申请日：1998-02-05
申请人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
发明人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
IPC分类号： G01N33/48 , B01L3/00 , B01L7/00 , C12M1/00 , C12Q1/68 , G01N27/447 , G01N33/50 , G01N33/53 , G01N33/569 , C07H21/00 , C12P19/34
CPC分类号： G01N27/44743 , B01L3/50273 , B01L3/502753 , B01L3/502761 , G01N27/44791 , G01N33/5002 , G01N33/569 , B01F13/0059 , B01L2200/0631 , B01L2200/0668 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0877 , B01L2400/0409 , B01L2400/0415 , B01L2400/0421 , B01L7/52
摘要： Integrated microfluidic devices comprising at least an enrichment channel and a main electrophoretic flowpath are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet. The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.
摘要翻译： 提供包括至少富集通道和主电泳流路的集成微流体装置。 在目标集成装置中，富集通道和主电泳流路被定位成使得废液通过排放口离开所述主电泳流路。 主题装置可用于各种电泳应用，包括临床测定，基因组学和药物应用的高通量筛选，点对点护理体外诊断，分子遗传分析和核酸诊断，细胞分离和生物研究。
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公开(公告)号：US06613525B2
公开(公告)日：2003-09-02
申请号：US10051847
申请日：2002-01-17
申请人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
发明人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
IPC分类号： C12Q168
CPC分类号： G01N27/44743 , B01F13/0059 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0877 , B01L2400/0409 , B01L2400/0415 , B01L2400/0421 , G01N27/44791 , G01N33/5002 , G01N33/569
摘要： Integrated microfluidic devices comprising at least an enrichment channel (10) and a main electrophoretic flowpath (12) are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet (6). The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.
摘要翻译： 提供包括至少富集通道（10）和主电泳流路（12）的集成微流体装置。 在主题集成装置中，富集通道和主电泳流路被定位成使得废液通过排出口（6）离开所述主电泳流路。 主题装置可用于各种电泳应用，包括临床测定，基因组学和药物应用的高通量筛选，点对点护理体外诊断，分子遗传分析和核酸诊断，细胞分离和生物研究。
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公开(公告)号：US06344326B1
公开(公告)日：2002-02-05
申请号：US09501706
申请日：2000-02-10
申请人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
发明人： Robert J. Nelson , Herbert H. Hooper , Alan K. Hauser , Sharat Singh , Stephen J. Williams , Alexander P. Sassi
IPC分类号： C07H2104
CPC分类号： G01N27/44743 , B01F13/0059 , B01L3/50273 , B01L3/502753 , B01L3/502761 , B01L7/52 , B01L2200/0631 , B01L2200/0668 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0877 , B01L2400/0409 , B01L2400/0415 , B01L2400/0421 , G01N27/44791 , G01N33/5002 , G01N33/569
摘要： Integrated microfluidic devices comprising at least an enrichment channel (10) and a main electrophoretic flowpath (12) are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet (6). The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.
摘要翻译： 提供包括至少富集通道（10）和主电泳流路（12）的集成微流体装置。 在主题集成装置中，富集通道和主电泳流路被定位成使得废液通过排出口（6）离开所述主电泳流路。 主题装置可用于各种电泳应用，包括临床测定，基因组学和药物应用的高通量筛选，点对点护理体外诊断，分子遗传分析和核酸诊断，细胞分离和生物研究。
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公开(公告)号：US6007690A
公开(公告)日：1999-12-28
申请号：US902855
申请日：1997-07-30
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44791 , G01N27/44743 , B01F13/0059
摘要： Integrated microfluidic devices comprising at least an enrichment channel and a main electrophoretic flowpath are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet. The subject devices find use in a variety of electrophoretic applications, including clinical assays, high throughput screening for genomics and pharmaceutical applications, point-or-care in vitro diagnostics, molecular genetic analysis and nucleic acid diagnostics, cell separations, and bioresearch generally.
摘要翻译： 提供包括至少富集通道和主电泳流路的集成微流体装置。 在目标集成装置中，富集通道和主电泳流路被定位成使得废液通过排放口离开所述主电泳流路。 主题装置可用于各种电泳应用，包括临床测定，基因组学和药物应用的高通量筛选，点对点护理体外诊断，分子遗传分析和核酸诊断，细胞分离和生物研究。
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公开(公告)号：US5885432A
公开(公告)日：1999-03-23
申请号：US589150
申请日：1996-01-22
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44747
摘要： Electrophoretic media are provided comprising at least un-crosslinked polymers which have a temperature reversible transition from low viscosity to high viscosity, so as to be pourable at one temperature, while providing sieving properties at another temperature. Optionally included with the un-crosslinked polymers having a reversible temperature responsive viscosity change are gelling agents and polymers which do not have the reversible temperature responsive viscosity change. The subject compositions have excellent clarity, provide excellent separation and resolution, handling properties, and mechanical strength. The compositions are compatible with transfer of separated components from the gel to an accepting membrane.
摘要翻译： 提供的电泳介质包含至少未交联的聚合物，其具有从低粘度到高粘度的温度可逆转变，以便在一个温度下可倾倒，同时在另一个温度下提供筛分特性。 任选地与具有可逆温度响应粘度变化的未交联聚合物一起包括不具有可逆温度响应粘度变化的胶凝剂和聚合物。 主题组合物具有优异的透明度，提供优异的分离和分辨率，处理性能和机械强度。 组合物与分离的组分从凝胶转移到接受膜是相容的。
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6.发明授权Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis 失效包含线性共聚物的热可逆水凝胶及其在电泳中的应用
公开(公告)号：US5883211A
公开(公告)日：1999-03-16
申请号：US636599
申请日：1996-04-23
IPC分类号： B03C5/00 , B01D57/02 , C08F220/60 , C08F226/00 , C08F251/00 , C08F271/02 , C25B7/00 , G01N27/447 , C08F220/56 , C08F222/38
CPC分类号： G01N27/44747 , C08F220/60 , C08F251/00 , C08F271/02 , Y10S524/916
摘要： Thermoreversible hydrogels comprising non-ionic, uncrosslinked copolymers, and methods of their use in electrophoresis, are provided. The subject copolymers comprise polyacrylamide backbones, where a portion of the acrylamide monomeric units comprise hydrogen bonding groups as N-substituents. Combination of the subject copolymers with an aqueous phase provides thermoreversible hydrogels which find use as separation media in electrophoretic applications.
摘要翻译： 提供了包含非离子，未交联的共聚物的热可逆水凝胶及其在电泳中的应用。 主题共聚物包括聚丙烯酰胺主链，其中丙烯酰胺单体单元的一部分包含作为N-取代基的氢键基团。 本发明共聚物与水相的组合提供热可逆水凝胶，其可用作电泳应用中的分离介质。
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7.发明授权Thermoreversible hydrogels comprising linear copolymers and their use in electrophoresis 失效包含线性共聚物的热可逆水凝胶及其在电泳中的应用
公开(公告)号：US5631337A
公开(公告)日：1997-05-20
申请号：US589026
申请日：1996-01-19
IPC分类号： B03C5/00 , B01D57/02 , C08F220/60 , C08F226/00 , C08F251/00 , C08F271/02 , C25B7/00 , G01N27/447 , C08F220/56 , C08F222/38
CPC分类号： G01N27/44747 , C08F220/60 , C08F251/00 , C08F271/02
摘要： Thermoreversible hydrogels comprsing non-ionic, linear copolymers, and methods of their use in electrophoresis, are provided. The subject copolymers comprise polyacrylamide backbones, where a portion of the acrylamide monomeric units compose hydrogen bonding groups as N-substituents. Combination of the subject copolymers with an aqueous phase provides thermoreversible hydrodgels which find use as separation media in electrophoretic applications.
摘要翻译： 提供了包含非离子型线型共聚物的热可逆水凝胶及其在电泳中的应用。 主题共聚物包括聚丙烯酰胺主链，其中一部分丙烯酰胺单体单元组成氢键基团作为N-取代基。 本发明共聚物与水相的组合提供热可逆水凝胶，其可用作电泳应用中的分离介质。
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公开(公告)号：US5770029A
公开(公告)日：1998-06-23
申请号：US690307
申请日：1996-07-30
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44791 , G01N27/44743 , B01F13/0059
摘要： Integrated electrophoretic microdevices comprising at least an enrichment channel and a main electrophoretic flowpath are provided. In the subject integrated devices, the enrichment channel and the main electrophoretic flowpath are positioned so that waste fluid flows away from said main electrophoretic flowpath through a discharge outlet. The subject devices find use in a variety of electrophoretic applications, including clinical assays.
摘要翻译： 提供了至少包括富集通道和主电泳流路的集成电泳微型器件。 在目标集成装置中，富集通道和主电泳流路被定位成使得废液通过排放口离开所述主电泳流路。 主题装置可用于各种电泳应用，包括临床测定。
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公开(公告)号：US06176962B1
公开(公告)日：2001-01-23
申请号：US08878437
申请日：1997-06-18
IPC分类号： B32B3100
CPC分类号： B01D57/02 , B01F13/0059 , B01F13/0076 , B01J19/0093 , B01L3/502707 , B01L2200/0689 , B01L2200/12 , B01L2300/0816 , B01L2300/0887 , B01L2400/0415 , B29C65/02 , B29C65/1403 , B29C65/1406 , B29C65/1412 , B29C65/1425 , B29C65/48 , B29C65/4815 , B29C65/483 , B29C65/4835 , B29C65/4845 , B29C65/485 , B29C65/4865 , B29C65/524 , B29C66/20 , B29C66/5346 , B29C66/54 , B29C66/71 , B29C66/73117 , B29L2031/756 , B29L2031/757 , B82Y10/00 , B82Y40/00 , C07K1/26 , G01N27/44704 , G01N27/44743 , G01N27/44752 , G01N27/44773 , G01N27/44791 , G03F7/0002 , B29K2083/00 , B29K2067/003 , B29K2033/12 , B29K2033/08 , B29K2023/06
摘要： Methods are provided for the fabrication of polymeric microchannel structures having enclosed microchannels of capillary dimension. The microchannel structures are constructed of a base plate and a cover, sealed together. Microchannel structures having walls of a plastic material are formed in a generally planar surface of at least the base plate. The cover has at least one generally planar surface, and the microchannel structures are enclosed by bonding the planar surfaces of the cover and the base plate together. In some embodiments the surfaces of the cover and base plate are both of plastic material, and are directly thermally bonded. In some embodiments a bonding material is applied to one of the surface prior to bringing the surfaces together. Suitable bonding materials are disclosed.
摘要翻译： 提供了用于制造具有毛细管尺寸的封闭微通道的聚合物微通道结构的方法。 微通道结构由基板和盖构成，密封在一起。 具有塑料材料壁的微通道结构形成在至少基板的大致平坦的表面上。 盖具有至少一个大致平坦的表面，并且通过将盖和基板的平面表面粘合在一起来封闭微通道结构。 在一些实施例中，盖和基板的表面都是塑料材料，并且是直接热粘结的。 在一些实施例中，在使表面在一起之前，将粘合材料施加到该表面之一。 公开了合适的粘合材料。
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公开(公告)号：US6054034A
公开(公告)日：2000-04-25
申请号：US853661
申请日：1997-05-09
IPC分类号： B01D57/02 , B01F13/00 , B01J19/00 , B01L3/00 , B29C65/02 , B29C65/14 , C07K1/26 , G01N27/447 , G03F7/00 , G01N27/26
CPC分类号： B82Y10/00 , B01D57/02 , B01F13/0076 , B01J19/0093 , B01L3/502753 , B29C65/02 , B29C65/483 , B29C66/54 , B29C66/542 , B82Y40/00 , C07K1/26 , G01N27/44752 , G01N27/44773 , G01N27/44791 , G03F7/0002 , B01F13/0059 , B01L2200/0689 , B01L2200/12 , B01L2300/0816 , B01L2400/0415 , B29C65/1425 , B29C65/4835 , B29C65/4845 , B29C65/4865 , B29C65/524 , B29C66/71 , B29L2031/756 , B29L2031/757
摘要： Microchannels having at least an acrylic inner surface and methods of their use in electrophoretic applications are provided. The subject microchannels may be in the form of a variety of configurations suitable for holding an electrophoretic medium. The subject microchannels give rise to substantially reduced EOF and/or adsorption as compared to fused silica under conditions of electrophoresis and find use in a variety of electrophoretic applications in which charged entities are moved through a medium under the influence of the an applied electric field.
摘要翻译： 提供至少具有丙烯酸内表面的微通道及其在电泳应用中的应用。 受试者微通道可以是适于保持电泳介质的各种构型的形式。 与电泳条件下的熔融二氧化硅相比，本发明微通道导致显着降低的EOF和/或吸附，并且可用于各种电泳应用，其中带电实体在施加的电场的影响下移动通过介质。
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