Raman detection based flow cytometer
    5.
    发明授权
    Raman detection based flow cytometer 有权
    基于拉曼检测的流式细胞仪

    公开(公告)号:US07333197B2

    公开(公告)日:2008-02-19

    申请号:US10991001

    申请日:2004-11-17

    IPC分类号: G01N21/00

    CPC分类号: G01N21/658 G01N15/147

    摘要: A method and apparatus for enabling chemical identification of individual particles, cells of molecules by obtaining a Raman spectrum of a particle, cell or molecule as it flows past a sensing point in a flow cytometer. The particles, which may be cells or molecules, are associated with a suitable noble metal colloid or colloidal aggregate. Cellular particles may be associated with gold or silver colloidal particles by ultra-sonic sonification while in a sample preparation reservoir containing the gold or silver colloidal suspension. The colloid associated particles are then hydrodynamically focused into a single file by a fluid control module. The surface-enhance Raman Spectrum of individual particles are obtained by illuminating the particle with a laser as the particle flows past a sensing point and gathering the light that is non-elastically scattered (Raman scattered) by the particle. The surface-enhanced Raman spectrum is then analyzed to identify the particle.

    摘要翻译: 一种方法和装置,用于通过在流式细胞计数器中流过感测点时获得粒子,细胞或分子的拉曼光谱,从而能够化学鉴定单个颗粒,分子细胞。 可以是细胞或分子的颗粒与合适的贵金属胶体或胶体聚集体相关联。 在含有金或银胶体悬浮液的样品制备储存器中,细胞颗粒可以通过超声波超声处理与金或银胶体颗粒结合。 然后通过流体控制模块将胶体相关颗粒流体动力聚焦成单个文件。 当颗粒流过感测点并通过颗粒收集非弹性散射(拉曼散射)的光时,通过用激光照射颗粒来获得单个颗粒的表面增强拉曼光谱。 然后分析表面增强拉曼光谱以鉴定颗粒。