公开(公告)号：US20070181466A1

公开(公告)日：2007-08-09

申请号：US11595818

申请日：2006-11-09

申请人： Shan Wang ,  Nader Pourmand ,  Robert White

发明人： Shan Wang ,  Nader Pourmand ,  Robert White

IPC分类号： B03C1/30 ,  B03C1/00

CPC分类号： B03C1/0335 ,  B03C2201/18 ,  B03C2201/22

摘要： The present invention provides a magnetic sifter that is small in scale, enables three-dimensional flow in a direction normal to the substrate, allows relatively higher capture rates and higher flow rates, and provides a relatively easy method of releasing captured biomolecules. The magnetic sifter includes at least one substrate. Each substrate contains a plurality of slits, each of which extends through the substrate. The sifter also includes a plurality of magnets attached to the bottom surface of the substrate. These magnets are located proximal to the openings of the slits. An electromagnetic source controls the magnitude and direction of magnetic field gradient generated by the magnets. Either one device may be used, or multiple devices may be used in series. In addition, the magnetic sifter may be used in connection with a detection chamber.

摘要翻译： 本发明提供一种规模小的磁选筛机，使得能够沿垂直于基底的方向进行三维流动，允许相对较高的捕获率和较高的流速，并且提供了一种相对容易的释放捕获的生物分子的方法。 磁选机包括至少一个衬底。 每个基板包含多个狭缝，每个狭缝延伸穿过基板。 筛子还包括附着到基底的底表面的多个磁体。 这些磁体位于缝隙的开口附近。 电磁源控制由磁体产生的磁场梯度的大小和方向。 可以使用一个设备，或者可以串联使用多个设备。 此外，磁选机可以与检测室结合使用。

公开(公告)号：US20070065835A1

公开(公告)日：2007-03-22

申请号：US11231657

申请日：2005-09-20

申请人： Nader Pourmand ,  Ronald Davis ,  Shan Wang

发明人： Nader Pourmand ,  Ronald Davis ,  Shan Wang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68 ,  C12Q1/6837 ,  C12Q2565/519 ,  C12Q2537/1373 ,  C12Q2525/204

摘要： A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with. Next, a second probe having a determined length is hybridized to these duplexes. If the length of the target is greater than the length of the first probe it is displaced during this hybridization step by the process of branch migration. In contrast, if the length of the target is less than or equal to the length of the first probe, it is not displaced. Thus, the length of the polynucleotide target can be determined.

公开(公告)号：US20070287156A1

公开(公告)日：2007-12-13

申请号：US11789559

申请日：2007-04-24

申请人： Nader Pourmand ,  Ronald Davis ,  Shan Wang

发明人： Nader Pourmand ,  Ronald Davis ,  Shan Wang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6837 ,  C12Q2565/519 ,  C12Q2537/1373 ,  C12Q2525/204

摘要： A method of determining the length of a polynucleotide target is provided. With this method, a target is first hybridized to an array of first probes having different, determined lengths, resulting in the formation of duplexes between the polynucleotide target and the first probes. These duplexes have a single stranded section of target if the target is longer than the first probe it is in a duplex with, and a single stranded section of probe if the target is shorter than the first probe it is in a duplex with. Next, a series of probes is hybridized to the duplexes, breaking apart duplexes in which the target and probe have unequal lengths through the process of branch migration. Thus, the target only remains bound in the duplex if the target and probe are of equal lengths. The length of the polynucleotide target can thereby be determined.

摘要翻译： 提供了确定多核苷酸靶标长度的方法。 利用该方法，首先将靶与具有不同的确定长度的第一探针阵列杂交，导致在多核苷酸靶和第一探针之间形成双链体。 如果目标比第一探针长两倍以上，那么这些双链体具有目标的单链段，如果目标短于其双链体中的第一探针，则其具有探针的单链段。 接下来，一系列探针与双链体杂交，分离双链体，其中靶和探针通过分支迁移过程具有不等长度。 因此，如果目标和探针具有相等的长度，则目标仅保持在双工中。 因此可以确定多核苷酸靶的长度。

公开(公告)号：US20050100930A1

公开(公告)日：2005-05-12

申请号：US10829505

申请日：2004-04-22

申请人： Shan Wang ,  Robert White ,  Chris Webb ,  Guanxiong Li

发明人： Shan Wang ,  Robert White ,  Chris Webb ,  Guanxiong Li

IPC分类号： B01L3/00 ,  C12Q1/68 ,  C40B40/06 ,  C40B40/10 ,  C40B40/14 ,  C40B60/14 ,  C40B70/00 ,  G01N33/543 ,  G01N33/553 ,  H01F1/00 ,  H01F10/32

CPC分类号： H01F10/3272 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00563 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00655 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00734 ,  B01L3/5027 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y25/00 ,  B82Y30/00 ,  C12Q1/6834 ,  C40B40/06 ,  C40B40/10 ,  C40B40/14 ,  C40B60/14 ,  C40B70/00 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/553 ,  G01N2446/10 ,  H01F1/0054 ,  H01F10/3254 ,  Y10T428/2991 ,  C12Q2563/143

摘要： Magnetic nanoparticles and methods for their use in detecting biological molecules are disclosed. The magnetic nanoparticles can be attached to nucleic acid molecules, which are then captured by a complementary sequence attached to a detector, such as a spin valve detector or a magnetic tunnel junction detector. The detection of the bound magnetic nanoparticle can be achieved with high specificity and sensitivity.

摘要翻译： 公开了磁性纳米颗粒及其用于检测生物分子的方法。 磁性纳米颗粒可以连接到核酸分子，然后通过与检测器（例如自旋阀检测器或磁性隧道结检测器）连接的互补序列捕获。 可以以高特异性和灵敏度实现结合的磁性纳米颗粒的检测。

公开(公告)号：US20060008823A1

公开(公告)日：2006-01-12

申请号：US11125558

申请日：2005-05-10

申请人： Jennifer Kemp ,  Shan Wang ,  Chris Webb ,  Robert White

发明人： Jennifer Kemp ,  Shan Wang ,  Chris Webb ,  Robert White

IPC分类号： C12Q1/68

CPC分类号： C12Q1/683 ,  C12Q2525/151

摘要： The present invention provides methods for rapidly identifying and distinguishing between different DNA sequences utilizing short tandem repeat (STR) analysis and DNA microarrays. Specifically, these methods facilitate the deduction of a target molecule's identity, length, and number of STRs. In an embodiment, a labeled STR target sequence is hybridized to a DNA microarray carrying complementary probes. These probes vary in length to cover the range of possible STRs. The labeled single-stranded regions of the DNA hybrids are selectively removed from the microarray surface utilizing a post-hybridization enzymatic digestion. The number of repeats in the unknown target is deduced based on the pattern of target DNA that remains hybridized to the microarray. The DNA profiling techniques described herein are useful for performing forensic analysis to uniquely identify individual humans or other species.

摘要翻译： 本发明提供使用短串联重复（STR）分析和DNA微阵列快速鉴定和区分不同DNA序列的方法。 具体来说，这些方法有助于减少目标分子的身份，长度和STR的数量。 在一个实施方案中，标记的STR靶序列与携带互补探针的DNA微阵列杂交。 这些探头长度不同以覆盖可能的STR的范围。 利用后杂交酶消化，从微阵列表面选择性地除去DNA杂交体的标记的单链区域。 基于与微阵列杂交的目标DNA的模式，推导出未知目标中的重复数。 本文描述的DNA分析技术可用于执行法医分析以唯一地识别个体人或其他物种。

公开(公告)号：US20160310913A1

公开(公告)日：2016-10-27

申请号：US14849616

申请日：2015-09-10

申请人： Lixin Wu ,  Liang Yue ,  Shan Wang ,  Bao Li

发明人： Lixin Wu ,  Liang Yue ,  Shan Wang ,  Bao Li

IPC分类号： B01D71/80 ,  C08B37/16

CPC分类号： B01D71/80 ,  B01D2325/04 ,  C08B37/0015 ,  C08G83/007 ,  C08G83/008

摘要： The disclosure regards to the porous materials, concerning a flexible 2D single-layer supramolecular polymer and its application in precise nano size separation. It comprises the synthesis of a bolaform cationic molecule, preparation of a cationic bridging stick, a flexible 2D single-layer supramolecular polymer and supramolecular polymer membrane, and the application of the membrane in precise nano-size separation. A synergetic ionic self-assembly approach which is facile, convenient and based on the ionic bond without preferential direction is used to construct a flexible 2D single-layer supramolecular polymer. Furthermore, its distinctive properties such as uniform nanoporous structure and flexibility offer an unprecedented opportunity to fabricate ultrafiltration membrane towards precise nanosize separation.

摘要翻译： 本发明涉及多孔材料，涉及柔性2D单层超分子聚合物及其在精确纳米尺寸分离中的应用。 它包括合成阳离子阳离子分子，制备阳离子桥接棒，柔性2D单层超分子聚合物和超分子聚合物膜，以及膜在精确纳米尺寸分离中的应用。 使用易于，方便，基于没有优先方向的离子键的协同离子自组装方法构建柔性2D单层超分子聚合物。 此外，其独特的性质如均匀的纳米孔结构和柔性提供了前所未有的机会，以制造超滤膜，以实现精确的纳米级分离。

公开(公告)号：US20060088971A1

公开(公告)日：2006-04-27

申请号：US10975552

申请日：2004-10-27

申请人： Ankur Crawford ,  Henning Braunisch ,  Rajendran Nair ,  Gilroy Vandentop ,  Shan Wang

发明人： Ankur Crawford ,  Henning Braunisch ,  Rajendran Nair ,  Gilroy Vandentop ,  Shan Wang

IPC分类号： H01L21/20

CPC分类号： H01L23/645 ,  H01F17/0033 ,  H01F41/046 ,  H01L23/49822 ,  H01L28/10 ,  H01L2224/16 ,  H01L2224/16235 ,  H01L2924/00011 ,  H01L2924/00014 ,  H01L2924/01077 ,  H01L2924/01078 ,  H01L2924/15311 ,  H01L2924/15312 ,  H01L2924/3011 ,  H05K1/0373 ,  H05K1/165 ,  H05K3/4644 ,  H05K2201/086 ,  H05K2201/09563 ,  H01L2224/0401

摘要： An inductor structure comprised of a magnetic section and a single turn solenoid. The single turn solenoid to contain within a portion of the magnetic section and circumscribed by the magnetic section.

摘要翻译： 一个电感结构由一个磁性部分和一个单一的旋转螺线管组成。 单转螺线管包含在磁性部分的一部分内并由磁性部分限定。

公开(公告)号：US20060053971A1

公开(公告)日：2006-03-16

申请号：US10938897

申请日：2004-09-10

申请人： Shouheng Sun ,  Heng Yu ,  Shan Wang

发明人： Shouheng Sun ,  Heng Yu ,  Shan Wang

IPC分类号： B22F9/00

CPC分类号： C01G1/00 ,  B22F1/0022 ,  B22F1/0096 ,  B22F2999/00 ,  B82Y25/00 ,  B82Y30/00 ,  C01B17/20 ,  C01B19/007 ,  C01P2002/72 ,  C01P2002/84 ,  C01P2004/04 ,  C01P2004/30 ,  C01P2004/64 ,  C01P2004/80 ,  C01P2006/42 ,  C09C3/063 ,  H01F1/0054 ,  Y10S977/835 ,  B22F1/0018

摘要： Dumbbell-shaped or flower-shaped nanoparticles and a process of forming the same, wherein the process comprises forming a mixture of a nanoparticle with a precursor in a first solvent, wherein the nanoparticle comprises a hydrophobic outer coating; heating the mixture; cooling the mixture to room temperature; modifying the hydrophobic outer coating into a hydrophilic outer coating; precipitating a solid product from the mixture, and dispersing the product in a second solvent. The nanoparticles comprise any of a semiconducting, magnetic, and noble metallic material, wherein the nanoparticles comprise a first portion comprising any of PbSe, PbS, CdSe, CdS, ZnS, Au, Ag, Pd, and Pt, and wherein the precursor comprises any of a cationic, neutral or particulate Au, Ag, Pd, Pt, or transition metal (Fe, Co, Ni) precursors of Fe(CO)5, Co(CO)8, Ni(CO)4 or their analogues. The first and second solvents comprise any of alkanes, arenes, ethers, nitrites, ketones, and chlorinated hydrocarbons.

摘要翻译： 哑铃形或花形纳米颗粒及其形成方法，其中所述方法包括在第一溶剂中形成纳米颗粒与前体的混合物，其中所述纳米颗粒包含疏水外涂层; 加热混合物; 将混合物冷却至室温; 将疏水性外涂层改性为亲水性外涂层; 从混合物中沉淀出固体产物，并将产物分散在第二溶剂中。 纳米颗粒包括半导体，磁性和贵金属材料中的任何一种，其中纳米颗粒包括包含PbSe，PbS，CdSe，CdS，ZnS，Au，Ag，Pd和Pt中的任何一种的第一部分，并且其中前体包含任何 的阳离子，中性或微粒Au，Ag，Pd，Pt或过渡金属（Fe，Co，Ni）前体Fe（CO）5，Co（CO） （CO）4或它们的类似物。 第一和第二溶剂包括任何烷烃，芳烃，醚，亚硝酸盐，酮和氯代烃。

公开(公告)号：US20050258821A1

公开(公告)日：2005-11-24

申请号：US11128105

申请日：2005-05-11

申请人： Shan Wang ,  Shu-Jen Han

发明人： Shan Wang ,  Shu-Jen Han

IPC分类号： G01N33/543 ,  G01R33/00 ,  G01R33/09 ,  G01R33/12

CPC分类号： G01R33/09 ,  B82Y25/00 ,  G01N33/5438 ,  G01R33/093 ,  G01R33/12 ,  G01R33/1269 ,  Y10T436/13

摘要： A magnetic sensor array including magnetoresistive sensor elements having outputs combined by frequency division multiplexing (FDM) is provided. Each sensor element provides an input to a mixer which provides a distinct frequency shift. Preferably, time division multiplexing is also used to combine sensor element outputs. Each sensor element is typically in proximity to a corresponding sample. The sensor elements are preferably subarrays having row and column addressable sensor element pixels. This arrangement provides multiple sensor pixels for each sample under test. Multiplexing of sensor element outputs advantageously reduces readout time. A modulated external magnetic field is preferably applied during operation, to reduce the effect of 1/f noise on the sensor element signals. The effect of electromagnetic interference (EMI) induced by the magnetic field on sensor element signals is advantageously reduced by the mixing required for FDM.

摘要翻译： 提供了包括具有通过频分复用（FDM）组合的输出的磁阻传感器元件的磁传感器阵列。 每个传感器元件向混合器提供输入，其提供不同的频移。 优选地，时分复用也用于组合传感器元件输出。 每个传感器元件通常接近相应的样品。 传感器元件优选地具有行和列可寻址传感器元件像素的子阵列。 这种布置为每个待测样品提供了多个传感器像素。 传感器元件输出的多路复用有利于减少读出时间。 优选在操作期间施加调制的外部磁场，以减少1 / f噪声对传感器元件信号的影响。 通过FDM所需的混合有利地减小了由磁场引起的电磁干扰（EMI）对传感器元件信号的影响。

公开(公告)号：US20130275649A1

公开(公告)日：2013-10-17

申请号：US13514291

申请日：2012-05-16

申请人： Yan-Song Zhang ,  Shan Wang ,  Xuan Zhou ,  Min Jiao ,  Zhan-Wei Wang

发明人： Yan-Song Zhang ,  Shan Wang ,  Xuan Zhou ,  Min Jiao ,  Zhan-Wei Wang

IPC分类号： G06F12/10

CPC分类号： G06F12/1045 ,  G06F2212/653

摘要： An access optimization method for a main memory database based on page-coloring is described. An access sequence of all data pages of a weak locality dataset is ordered by page-color, and all the data pages are grouped by page-color, and then all the data pages of the weak locality dataset are scanned in a sequence of page-color grouping. Further, a number of memory pages having the same page-color are preset as a page-color queue, in which the page-color queue serves as a memory cache before a memory page is loaded into a CPU cache; the data page of the weak locality dataset first enters the page-color queue in an asynchronous mode, and is then loaded into the CPU cache to complete data processing. Accordingly, cache conflicts between datasets with different data locality strengths can be effectively reduced.

摘要翻译： 描述了基于页面着色的主存储数据库的访问优化方法。 弱位置数据集的所有数据页的访问序列按页面颜色排序，所有数据页按页面颜色分组，然后按照页面颜色顺序扫描弱位置数据集的所有数据页面， 颜色分组。 此外，将具有相同页面颜色的多个存储器页面预设为页面颜色队列，其中在将存储器页面加载到CPU高速缓存之前，页面颜色队列用作存储器高速缓存; 弱位置数据集的数据页首先以异步模式进入页面颜色队列，然后加载到CPU缓存中以完成数据处理。 因此，可以有效地减少具有不同数据局部性强度的数据集之间的缓存冲突。