摘要:
Disclosed is a liquid sending method using a sample processing chip having a flow path into which a plurality of liquids flow, and the method includes: sending, into the flow path, a first liquid held in a liquid holding portion provided in the sample processing chip by applying pressure to the liquid holding portion; sending, into the flow path, a second liquid in a storage portion provided in a liquid sending device connected to the sample processing chip, through an injection hole provided in the sample processing chip, by applying pressure to the storage portion; and forming, in the flow path, a fluid containing the first liquid having been sent from the liquid holding portion, and the second liquid having been sent through the injection hole.
摘要:
Disclosed are a novel means capable of accurately calculating the amount of in-vivo components as a test substance, and its uses. A sensor assembly 20 includes an electrochemical sensor 30 for measuring a test substance percutaneously extracted from a living body, and a processor 115a for calculating the intensity of the background signal at the measurement time point of the test substance after the elapse of the predetermined time based on the change over time of the intensity of the background signal measured within a predetermined time before measurement of the test substance by the electrochemical sensor 30. In this way, the amount of the in-vivo component as the test substance can be accurately calculated since the calculated value of the intensity of the background signal to be subtracted from the unprocessed signal including the signal attributable to the test substance can be obtained.
摘要:
In a hydrogen peroxide electrode 100 used as a working electrode 30 of an enzyme sensor 20, an intermediate layer 101 containing particles and a retentive substance for holding the particles inside the retentive substance are provided on a substrate 21, and an electrode layer 107 is provided on the intermediate layer 101. In this way, according to the hydrogen peroxide electrode 100, hydrogen peroxide can be detected with high sensitivity even when an applied voltage that is not susceptible to interference by interference substances present in the living body is used.
摘要:
Provided is a method for detecting a test substance. In this method, metal is deposited or a complex containing a test substance and a metal particle is immobilized on a working electrode on an electrode substrate including the working electrode and a counter electrode. An oxidation potential is applied to the working electrode to generate metal ions, then a reduction potential is applied to a portion having an area smaller than an area of the portion to which an oxidation potential is applied in the working electrode to deposit metal on the surface of the portion to which the reduction potential is applied, and current, voltage or charge caused by the metal deposited is measured to detect metal ions or a test substance.
摘要:
The present invention relates to a perfusion device and a perfusion method for culturing an undifferentiated cell using an organ or tissue extracted from a living body.
摘要:
Disclosed is a method for obtaining differentiated cells and/or differentiated cell products, including the steps of: introducing undifferentiated cells into a perfused organ or living tissue; subjecting the undifferentiated cells introduced to perfusion culture together with the organ or living tissue so as to allow the undifferentiated cells to differentiate, thereby obtaining differentiated cells and/or differentiated cell products; collecting a perfusion culture solution that contains the resulting differentiated cells and/or differentiated cell products; and obtaining the differentiated cells and/or differentiated cell products contained in the collected perfusion culture solution.
摘要:
A method for electrochemically detecting an analyte contained in a sample using a working electrode and a counter electrode, comprising: allowing an electrolyte solution which contains a solution prepared by dissolving an imidazolium iodide compound in a protic solvent to be into contact with the working electrode and the counter electrode; and electrochemically detecting the analyte contained in the sample in the presence of the electrolyte solution is disclosed. A kit to be used for the method for electrochemically detecting an analyte is also disclosed.
摘要:
A blood collection device including a flow path to flow blood by capillary action capable of collecting a large amount of blood is provided. The blood collection device includes an introduction inlet 23 for introducing the blood of the subject M, a substrate 20 connected to the introduction inlet 23 and including a flow path 25 to flow the blood by capillary action, and a cooling body 30 for cooling the flow path 25.
摘要:
Disclosed is an organ perfusion method including introducing a perfusion solution into an organ extracted from the living body and deriving the perfusion solution from the organ, wherein the organ and the perfusion solution are brought into contact with each other while applying oscillation to the organ.
摘要:
Disclosed is a method of detecting a test substance contained in a sample using a working electrode and a counter electrode. The method comprises (A) forming a complex on the working electrode, the complex comprising the test substance and a metal particle; (B) washing the working electrode; (C) adding a measurement solution to the working electrode and measuring a current, voltage or electric charge from the metal on the working electrode in the measurement solution by electrochemical measurement; and (D) detecting the test substance in the sample based on a measurement result obtained in the step (C). In the method, the step (B) comprises washing the working electrode using a measurement solution having the same composition as the composition of the measurement solution used in the step (C). In the method, pH of the measurement solution is set, based on zeta potentials of the metal particle and the working electrode, to pH in which no electrostatic interaction occurs between the metal particle and the working electrode or pH in which an electrostatic repulsive force occurs between the metal particle and the working electrode.