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    siRNA based on RNA sequence of SARS-CoV-2 and use thereof
    1.
    发明申请
    siRNA based on RNA sequence of SARS-CoV-2 and use thereof 有权

    公开(公告)号：US20230047473A1

    公开(公告)日：2023-02-16

    申请号：US17811961

    申请日：2022-07-12

    申请人： TOAGOSEI CO., LTD. ,  KYOTO UNIVERSITY

    发明人： Tetsuhiko Yoshida ,  Nahoko Baileykobayashi ,  Kazuo Takayama ,  Rina Hashimoto ,  Yoshinori Yoshida

    IPC分类号： C12N15/113

    摘要： The present disclosure provides siRNA that suppresses proliferation of new coronaviruses (SARS-CoV-2). The siRNA disclosed herein includes: a sense strand; and an antisense strand. The sense strand includes a target sequence comprising 19 to 23 bases in which a base at a 5′ terminal is guanine (G) or cytosine (C), and an overhang comprising 2 to 4 bases added to a 3′ terminal side of the target sequence. The antisense strand includes a sequence complementary to the target sequence, and an overhang comprising 2 to 4 bases added to a 3′ terminal side of the complementary sequence. Here, at least a part of the target sequence contains at least a part of a base sequence encoding a signal peptide region of a spike protein (S protein) of SARS-CoV-2.

    Method for producing myocardial cells using synthetic peptide
    4.
    发明授权
    Method for producing myocardial cells using synthetic peptide 有权

    公开(公告)号：US11359180B2

    公开(公告)日：2022-06-14

    申请号：US15569887

    申请日：2016-04-28

    申请人： Toagosei Co., Ltd. ,  KYOTO UNIVERSITY

    发明人： Tetsuhiko Yoshida ,  Kenichi Tanaka ,  Nahoko Baileykobayashi ,  Yoshinori Yoshida

    IPC分类号： C12N5/16 ,  C07K14/00 ,  C07K14/47 ,  C07K14/475 ,  C07K14/575 ,  C12N5/0735 ,  C12N5/077 ,  C12N5/074

    摘要： Provided is a method for producing myocardial cells from pluripotent stem cells. The myocardial cell production method provided by the present invention includes supplying an artificially produced synthetic peptide to a cell culture that contains pluripotent stem cells. The synthetic peptide is a peptide that contains a myocardial cell differentiation-inducing peptide sequence that induces pluripotent stem cells into myocardial cells. The myocardial cell differentiation-inducing peptide sequence is an amino acid sequence selected from the group consisting of (i) an amino acid sequence constituting the signal peptide of any protein belonging to the amyloid precursor protein (APP) family, (ii) a partial amino acid sequence of the amino acid sequence according to (i), and (iii) a modified amino acid sequence from the amino acid sequence according to (i) or (ii).

    Method for sorting cardiomyocytes
    9.
    发明授权
    Method for sorting cardiomyocytes 有权

    公开(公告)号：US10538740B2

    公开(公告)日：2020-01-21

    申请号：US15127134

    申请日：2015-03-20

    申请人： KYOTO UNIVERSITY

    发明人： Yoshinori Yoshida ,  Hirohide Saito ,  Kenji Miki ,  Kei Endo ,  Seiya Takahashi

    IPC分类号： C12N5/077 ,  C12N5/00 ,  C12N5/0735 ,  A61K48/00

    摘要： An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.