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    High throughput multi-antigen microfluidic fluorescence immunoassays
    2.
    发明申请
    High throughput multi-antigen microfluidic fluorescence immunoassays 审中-公开
    高通量多抗原微流体荧光免疫测定

    公开(公告)号：US20060263818A1

    公开(公告)日：2006-11-23

    申请号：US11439288

    申请日：2006-05-22

    申请人： Axel Scherer ,  Emil Kartalov ,  W. Anderson ,  Clive Taylor

    发明人： Axel Scherer ,  Emil Kartalov ,  W. Anderson ,  Clive Taylor

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/34

    CPC分类号： G01N33/582 ,  B01L3/502707 ,  B01L3/50273 ,  B01L3/502738 ,  B01L2200/0605 ,  B01L2200/0621 ,  B01L2300/0636 ,  B01L2300/0861 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655

    摘要： The development of a high-throughput multi-antigen microfluidic fluorescence immunoassay system is illustrated in a 100-chamber PDMS (polydimethylsiloxane) chip which performs up to 5 tests for each of 10 samples. Specificity of detection is demonstrated and calibration curves produced for C-Reactive Protein (CRP), Prostate Specific Antigen (PSA), ferritin, and Vascular Endothelial Growth Factor (VEGF). The measurements show sensitivity at and below levels that are significant in current clinical laboratory practice (with SIN>8 at as low as 10 pM antigen concentration). The chip uses 100 nL per sample for all four tests and provides an improved instrument for use in scientific research and “point-of-care” testing in medicine.

    摘要翻译： 高通量多抗原微流体荧光免疫测定系统的开发在100个腔室PDMS（聚二甲基硅氧烷）芯片中进行了说明，该芯片对10个样品中的每一个进行多达5次测试。 证明了检测的特异性，并为C-反应蛋白（CRP），前列腺特异性抗原（PSA），铁蛋白和血管内皮生长因子（VEGF）产生了校准曲线。 测量显示当前临床实验室实践中显着的敏感度和水平以下（SIN> 8，低至10 pM抗原浓度）。 该芯片用于所有四个测试，每个样品使用100nL，并提供用于医学科学研究和“点护理”测试的改进仪器。

    Online service switching and customizations
    4.
    发明申请
    Online service switching and customizations 审中-公开
    在线服务切换和定制

    公开(公告)号：US20050262449A1

    公开(公告)日：2005-11-24

    申请号：US10838645

    申请日：2004-05-03

    申请人： W. Anderson ,  Mark Radcliffe

    发明人： W. Anderson ,  Mark Radcliffe

    IPC分类号： H04L29/06 ,  H04L29/08 ,  G06F3/00

    CPC分类号： G06F3/0484 ,  G06F16/9566 ,  H04L29/06027 ,  H04L65/4084 ,  H04L65/604 ,  H04L67/02 ,  H04L67/306 ,  H04L67/36 ,  H04L69/329

    摘要： A system and methods enable switching between a plurality of online media services from within an application, such as a media player application. A user can switch to any one of a number of online services made available in a services menu and thereby make that service the active service. The active service has customization opportunities that permit the service to customize parts of the media player or other application. Advantages include providing online media services with an ability to control the way in which customers discover and purchase media from within the feature rich environment of various user applications such as a PC-based media player application. From a user's perspective, the advantages include an uninterrupted media experience that can involve the entire process of discovering, purchasing and using of a wide variety of media content all from within the feature rich environment of a media player application.

    摘要翻译： 系统和方法能够在诸如媒体播放器应用之类的应用内从多个在线媒体服务之间进行切换。 用户可以切换到在服务菜单中可用的多个在线服务中的任何一个，从而使该服务成为主动服务。 主动服务具有定制机会，允许服务定制媒体播放器或其他应用程序的部分。 优点包括提供在线媒体服务，能够控制客户在诸如基于PC的媒体播放器应用的各种用户应用的特征丰富的环境内发现和购买媒体的方式。 从用户的角度来看，优点包括不间断的媒体体验，其可以涉及从媒体播放器应用的特征丰富的环境内发现，购买和使用各种媒体内容的整个过程。

    Retroviral vectors including modified envelope escort protein
    5.
    发明申请
    Retroviral vectors including modified envelope escort protein 审中-公开
    逆转录病毒载体包括修饰的包膜保护蛋白

    公开(公告)号：US20060292121A1

    公开(公告)日：2006-12-28

    申请号：US11452083

    申请日：2006-06-12

    申请人： Frederick Hall ,  Erlinda Gordon ,  W. Anderson

    发明人： Frederick Hall ,  Erlinda Gordon ,  W. Anderson

    IPC分类号： A61K48/00 ,  C12N15/867

    CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N2740/13043 ,  C12N2740/13045

    摘要： A retroviral vector comprising a first retroviral envelope protein and at least one modified retroviral envelope protein, wherein said first retroviral envelope protein includes a surface protein comprising (i) a receptor binding region; (ii) a hypervariable polyproline region; and (iii) a body portion, and said modified retroviral envelope protein, prior to modification, includes a surface protein which includes (i) a receptor binding region; (ii) a hypervariable polyproline region; and (iii) a body portion, characterized in that said modified retroviral envelope protein has been modified such that at least 90% of the amino acid residues of the receptor binding region of said surface protein of said modified retroviral envelope protein have been removed and replaced with a non-retroviral protein or peptide.

    摘要翻译： 包含第一逆转录病毒包膜蛋白和至少一种修饰的逆转录病毒包膜蛋白的逆转录病毒载体，其中所述第一逆转录病毒包膜蛋白包括表面蛋白，其包含（i）受体结合区; （ii）高变脯氨酸区域; 和（iii）身体部分，并且所述修饰的逆转录病毒包膜蛋白在修饰之前包括表面蛋白，其包括（i）受体结合区; （ii）高变脯氨酸区域; 其特征在于所述修饰的逆转录病毒包膜蛋白已被修饰，使得所述修饰的逆转录病毒包膜蛋白的所述表面蛋白的受体结合区的至少90％的氨基酸残基被去除和替换 与非逆转录病毒蛋白或肽。

    Microfluidic large scale integration
    6.
    发明申请
    Microfluidic large scale integration 有权
    微流控大规模集成

    公开(公告)号：US20050072946A1

    公开(公告)日：2005-04-07

    申请号：US10915960

    申请日：2004-08-10

    申请人： Vincent Studer ,  Stephen Quake ,  W. Anderson ,  Sebastian Maerkl

    发明人： Vincent Studer ,  Stephen Quake ,  W. Anderson ,  Sebastian Maerkl

    IPC分类号： F16K31/00

    CPC分类号： F15C1/06 ,  G06F17/5018 ,  G06F2217/16 ,  Y10T137/2224

    摘要： Using basic physical arguments, a design and method for the fabrication of microfluidic valves using multilayer soft lithography is presented. Embodiments of valves in accordance with the present invention feature elastomer membrane portions of substantially constant thickness, allowing the membranes to experience similar resistance to an applied pressure across their entire width. Such on-off valves fabricated with upwardly- or downwardly-deflectable membranes can have extremely low actuation pressures, and can be used to implement active functions such as pumps and mixers in integrated microfluidic chips. Valve performance was characterized by measuring both the actuation pressure and flow resistance over a wide range of design parameters, and comparing them to both finite element simulations and alternative valve geometries.

    摘要翻译： 使用基本的物理参数，提出了使用多层软光刻制造微流体阀的设计和方法。 根据本发明的阀的实施例具有基本上恒定厚度的弹性体膜部分，允许膜在其整个宽度上经受类似的施加压力的阻力。 用向上或向下可偏转的膜制造的这种开关阀可以具有非常低的致动压力，并且可以用于实现积分功能，例如集成微流体芯片中的泵和混合器。 阀性能的特征在于在宽范围的设计参数下测量致动压力和流动阻力，并将其与有限元模拟和替代阀几何形状进行比较。

    Microfluidic nucleic acid analysis
    7.
    发明申请
    Microfluidic nucleic acid analysis 有权
    微流控核酸分析

    公开(公告)号：US20050053952A1

    公开(公告)日：2005-03-10

    申请号：US10678946

    申请日：2003-10-02

    申请人： Jong Hong ,  Vincent Studer ,  W. Anderson ,  Stephen Quake ,  Jared Leadbetter

    发明人： Jong Hong ,  Vincent Studer ,  W. Anderson ,  Stephen Quake ,  Jared Leadbetter

    IPC分类号： B01L3/00 ,  C12M1/34 ,  C12Q1/68

    CPC分类号： B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2565/629

    摘要： Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    摘要翻译： 来自从各种环境取样的细胞和病毒的核酸可以利用微流体技术纯化和表达。 根据本发明的一个实施方案，可通过稀释，分选和/或分割在微流体室中分离单个或小组的细胞或病毒。 分离的细胞或病毒可以直接在微流体室中裂解，并且通过暴露于亲和珠来纯化得到的核酸。 随后洗脱纯化的核酸之后可以连接和细胞转化，全部在相同的微流体芯片内。 在一个具体应用中，细胞分离，裂解和核酸纯化可以使用高度并行的微流体结构来构建gDNA和cDNA文库。

    Expression Of Cyclin G1 In Tumors
    9.
    发明申请
    Expression Of Cyclin G1 In Tumors 失效
    细胞周期蛋白G1在肿瘤中的表达

    公开(公告)号：US20070172486A1

    公开(公告)日：2007-07-26

    申请号：US11553416

    申请日：2006-10-26

    申请人： Erlinda Gordon ,  Frederick Hall ,  W. Anderson

    发明人： Erlinda Gordon ,  Frederick Hall ,  W. Anderson

    IPC分类号： A61K48/00 ,  A61K39/395 ,  C12N15/861 ,  C12Q1/68 ,  G01N33/574

    CPC分类号： C12N15/113 ,  A01K67/0271 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/058 ,  A01K2227/105 ,  A01K2267/0331 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4738 ,  C07K14/4746 ,  C07K16/18 ,  C12N2799/027 ,  G01N2333/4739

    摘要： A method of treating a tumor (in particular osteosarcoma or Ewing's sarcoma) in a host by administering to a host or to the tumor cells an agent which inhibits cyclin G1 protein in an amount effective to inhibit cyclin G1 protein in tumor cells of the host. The agent may be an antisense polynucleotide which is complementary to at least a portion of a polynucleotide encoding cyclin G1 protein, or an antibody or fragment or derivative thereof which recognizes cyclin G1 protein. Also contemplated within the scope of the present invention are (i) the immortalization of cell lines by transducing cells with a polynucleotide encoding cyclin G1 protein; (ii) increasing the receptiveness of cells to retroviral infection by transducing cells with a polynucleotide encoding cyclin G1 protein; and (iii) the detection of cancer by detecting cyclin G1 protein or a polynucleotide encoding cyclin G1 protein in cells. In addition, the present invention provides expression vehicles, such as, for example, retroviral vectors and adenoviral vectors, which include polynucleotides which encode agents which inhibit cyclin G1 protein, and expression vehicles which include a polynucleotide encoding cyclin G1 protein.

    摘要翻译： 通过向宿主或肿瘤细胞施用以有效抑制宿主肿瘤细胞中的细胞周期蛋白G1蛋白的量的细胞周期蛋白G1蛋白质的药物来治疗宿主中的肿瘤（特别是骨肉瘤或尤因氏肉瘤）的方法。 该试剂可以是与编码细胞周期蛋白G1蛋白的多核苷酸的至少一部分互补的反义多核苷酸，或其识别细胞周期蛋白G1蛋白的抗体或其片段或衍生物。 在本发明的范围内也考虑到：（i）通过用编码细胞周期蛋白G1蛋白的多核苷酸转导细胞来使细胞系永生化; （ii）通过用编码细胞周期蛋白G1蛋白的多核苷酸转导细胞来增加细胞对逆转录病毒感染的接受性; 和（iii）通过检测细胞周期蛋白G1蛋白或编码细胞周期蛋白G1蛋白的多核苷酸来检测癌症。 此外，本发明提供表达载体，例如逆转录病毒载体和腺病毒载体，其包括编码抑制细胞周期蛋白G1蛋白的试剂的多核苷酸，以及包含编码细胞周期蛋白G1蛋白的多核苷酸的表达载体。

    Microfluidic autoregulator devices and arrays for operation with newtonian fluids
    10.
    发明申请
    Microfluidic autoregulator devices and arrays for operation with newtonian fluids 有权
    用于牛顿流体操作的微流控自动调节器和阵列

    公开(公告)号：US20070119510A1

    公开(公告)日：2007-05-31

    申请号：US11606312

    申请日：2006-11-28

    申请人： Emil Kartalov ,  Axel Scherer ,  W. Anderson

    发明人： Emil Kartalov ,  Axel Scherer ,  W. Anderson

    IPC分类号： F15C1/04

    CPC分类号： G05D7/0694 ,  B01L3/5027 ,  F16K99/0001 ,  F16K99/0015 ,  F16K99/0026 ,  F16K99/0057 ,  F16K2099/008 ,  F16K2099/0084 ,  Y10T137/218 ,  Y10T137/2202 ,  Y10T137/7796

    摘要： By use of the vias a microfluidic autoregulator is fabricated comprising an origin of a fluid, a sink for the fluid, a main flow channel coupling the origin and the sink, a valve communicated to the main flow channel to selectively control flow of fluid therethrough, and means dependent on flow through the main flow channel for creating a pressure differential across the valve to at least partially activate the valve to control flow of fluid through the main flow channel. The means for dependent on flow for creating a pressure differential comprises either a dead-end detour channel from the flow channel to the valve, or a loop channel fed back to the control chamber of the valve.

    摘要翻译： 通过使用通孔，制造微流体自动调节器，该微流控自动调节器包括流体的来源，用于流体的水槽，连接原点和水槽的主流动通道，连通到主流动通道的阀，以选择性地控制流体的流动， 并且取决于通过主流动通道的流动的装置，用于在阀门两端产生压差，至少部分地启动阀门，以控制通过主流动通道的流体流动。 用于产生压力差的用于依赖于流动的装置包括从流动通道到阀门的死端迂回通道或反馈到阀的控制室的回路通道。