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    Universal Probe Assay Methods
    3.
    发明申请
    Universal Probe Assay Methods 有权
    通用探针测定方法

    公开(公告)号：US20120115143A1

    公开(公告)日：2012-05-10

    申请号：US13280214

    申请日：2011-10-24

    申请人： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    发明人： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    摘要： Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, said first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    摘要翻译： 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并将第一检测探针与扩增产物杂交，所述第一检测探针包括与第一探针结合序列杂交的第一片段和与第二探针结合序列杂交的第二片段，从而产生标记的扩增产物 。

    Universal probe assay methods
    4.
    发明授权
    Universal probe assay methods 有权
    通用探针测定方法

    公开(公告)号：US09353406B2

    公开(公告)日：2016-05-31

    申请号：US13280214

    申请日：2011-10-24

    申请人： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    发明人： Kenneth J. Livak ,  Jason A. A. West ,  Robert C. Jones

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/682 ,  C12Q1/6876 ,  C12Q2600/178 ,  C12Q2525/155 ,  C12Q2525/307 ,  C12Q2537/161

    摘要： Reagents and methods are provided for detecting the presence of a target polynucleotide in a sample are disclosed. In one aspect, a method for producing a labeled amplification product by amplifying a target nucleic acid sequence to produce an amplification product comprising the target sequence, a first probe-binding sequence 5′ to the target sequence, and a second probe-binding sequence 3′ to the target sequence, thereby producing an amplification product; and hybridizing a first detection probe to the amplification product, the first detection probe comprising a first segment that hybridizes to the first probe-binding sequence and a second segment that hybridizes to the second probe-binding sequence, thereby producing a labeled amplification product is disclosed.

    摘要翻译： 提供了用于检测样品中靶多核苷酸的存在的试剂和方法。 一方面，通过扩增靶核酸序列以产生包含靶序列的扩增产物，与靶序列的第一探针结合序列5'和第二探针结合序列3来产生标记的扩增产物的方法 '到靶序列，从而产生扩增产物; 并且将第一检测探针与扩增产物杂交，所述第一检测探针包含与第一探针结合序列杂交的第一区段和与第二探针结合序列杂交的第二区段，从而产生标记的扩增产物 。

    Multiplex Amplification for the Detection of Nucleic Acid Variations
    6.
    发明申请
    Multiplex Amplification for the Detection of Nucleic Acid Variations 审中-公开
    用于检测核酸变异的多重扩增

    公开(公告)号：US20130022973A1

    公开(公告)日：2013-01-24

    申请号：US13521400

    申请日：2011-01-14

    申请人： Carl L. G. Hansen ,  Oleh Petriv ,  Kevin Heyries ,  Kenneth J. Livak

    发明人： Carl L. G. Hansen ,  Oleh Petriv ,  Kevin Heyries ,  Kenneth J. Livak

    IPC分类号： C12Q1/68 ,  G01N21/64

    CPC分类号： C12Q1/6851 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2563/159

    摘要： Kits, primers, and methods are provided herein for detecting relative target source to reference source ratios in a biological sample, by distributing the biological sample into discrete subsamples, wherein the biological sample includes, a plurality of target molecules on a target source; and a plurality of reference molecules on a reference source; providing target primers directed to one or more of the plurality of target molecules and reference primers directed to one or more of the plurality of reference molecules; performing digital amplification with the target primers and the reference primers; and detecting the presence or absence of amplified target products with target probes and detecting the presence or absence of amplified reference products with reference probes, wherein the ratio of amplified target products to amplified reference products is indicative of a relative amount of target source to reference source in a biological sample.

    摘要翻译： 本文提供了试剂盒，引物和方法，用于通过将生物样品分配到离散的子样品中来检测生物样品中的相对目标来源参考源比例，其中生物样品包括目标源上的多个靶分子; 和参考源上的多个参考分子; 提供指向所述多个靶分子中的一个或多个的靶引物和指向所述多个参考分子中的一个或多个的参考引物; 用目标引物和参照引物进行数字扩增; 并用靶探针检测扩增的靶产物的存在或不存在，并用参考探针检测扩增的参考产物的存在或不存在，其中扩增的靶产物与扩增的参考产物的比率指示靶源与参考源的相对量 在生物样品中。

    Multiplexed Amplification of Short Nucleic Acids
    7.
    发明申请
    Multiplexed Amplification of Short Nucleic Acids 审中-公开
    短核酸的多重扩增

    公开(公告)号：US20110251083A1

    公开(公告)日：2011-10-13

    申请号：US12762272

    申请日：2010-04-16

    申请人： Kai Qin LAO ,  Kenneth J. Livak ,  Neil A. Straus

    发明人： Kai Qin LAO ,  Kenneth J. Livak ,  Neil A. Straus

    IPC分类号： C40B30/04 ,  C12P19/34 ,  C40B40/06 ,  C12Q1/68

    CPC分类号： C12N15/1065 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2525/301 ,  C12Q2525/161 ,  C12Q2563/179 ,  C12Q2537/143 ,  C12Q2525/207

    摘要： The present teachings provide methods, compositions, and kits for reverse transcribing and amplifying small nucleic acids such as micro RNAs. High levels of multiplexing are provided by the use of a zip-coded stem-loop reverse transcription primer, along with a PCR-based pre-amplification reaction that comprises a zip-coded forward primer. Detector probes in downstream decoding PCRs can take advantage of the zip-code introduced by the stem-loop reverse transcription primer. In some embodiments, further amplification is achieved by cycling the reverse transcription reaction. The present teachings also provide compositions and kits useful for performing the reverse transcription and amplification reactions described herein.

    摘要翻译： 本教导提供用于逆转录和扩增小核酸如微RNA的方法，组合物和试剂盒。 通过使用zip编码的茎 - 环逆转录引物以及包含经翻译的正向引物的基于PCR的预扩增反应来提供高水平的复用。 下游解码PCR中的检测器探针可以利用茎环逆转录引物引入的zip码。 在一些实施方案中，通过循环逆转录反应来实现进一步的扩增。 本教导还提供可用于进行本文所述的逆转录和扩增反应的组合物和试剂盒。

    Systems and Methods for Isolating Nucleic Acids
    10.
    发明申请
    Systems and Methods for Isolating Nucleic Acids 失效
    用于分离核酸的系统和方法

    公开(公告)号：US20090071830A1

    公开(公告)日：2009-03-19

    申请号：US12179455

    申请日：2008-07-24

    申请人： Charles S. Vann ,  Maxim G. Brevnov ,  David W. Ruff ,  Kenneth J. Livak

    发明人： Charles S. Vann ,  Maxim G. Brevnov ,  David W. Ruff ,  Kenneth J. Livak

    IPC分类号： B01D57/02 ,  B01D61/42 ,  C25B9/08

    CPC分类号： C12N15/101 ,  G01N27/44782

    摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.

    摘要翻译： 用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标 与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间 所述至少一个样品室。 该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场， 其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。