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公开(公告)号：US6048975A
公开(公告)日：2000-04-11
申请号：US800123
申请日：1997-02-13
摘要： A process for the chemical synthesis of oligonucleotidesUse of a dansylethoxycarbonyl group as base-labile 5'-hydroxyl protective group in the chemical synthesis of DNA and RNA and suitable synthetic processes.The ease of detection of the dansyl protective group and the ease of elimination from the sugar residue of the nucleotide without side reactions make oligonucleotide synthesis possible with high yields in very small quantities.
摘要翻译： 寡核苷酸的化学合成方法在DNA和RNA的化学合成中使用丹参乙氧基羰基作为碱不稳定的5'-羟基保护基团和合适的合成方法。 丹磺酰基保护基的易于检测和不含副反应的核苷酸的糖残基的易于消除使寡核苷酸合成成为非常少的高产率。
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公开(公告)号：US5945524A
公开(公告)日：1999-08-31
申请号：US487229
申请日：1995-06-07
IPC分类号： C12N15/09 , C07B51/00 , C07C315/04 , C07C317/36 , C07H19/04 , C07H19/06 , C07H19/067 , C07H19/073 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/167 , C07H19/173 , C07H19/20 , C07H19/207 , C07H19/23 , C07H21/00 , C07H21/02 , C07H21/04 , C07H1/00
CPC分类号： C07H21/00 , C07C315/04 , C07C317/36 , C07H19/10 , C07H19/16 , C07H19/20 , Y02P20/55
摘要： Use of a dansylethoxycarbonyl group as base-labile 5'-hydroxyl protective group in the chemical synthesis of DNA and RNA and suitable synthetic processes.The ease of detection of the dansyl protective group and the ease of elimination from the sugar residue of the nucleotide without side reactions make oligonucleotide synthesis possible with high yields in very small quantities.
摘要翻译： 在DNA和RNA的化学合成中使用丹酰乙氧基羰基作为碱不稳定的5'-羟基保护基团和合适的合成方法。 丹磺酰基保护基的易于检测和不含副反应的核苷酸的糖残基的易于消除使寡核苷酸合成成为非常少的高产率。
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3.发明授权
公开(公告)号：US5631362A
公开(公告)日：1997-05-20
申请号：US437566
申请日：1995-05-09
IPC分类号： C12N15/09 , C07B51/00 , C07C315/04 , C07C317/36 , C07H19/04 , C07H19/06 , C07H19/067 , C07H19/073 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/167 , C07H19/173 , C07H19/20 , C07H19/207 , C07H19/23 , C07H21/00 , C07H21/02 , C07H21/04 , C07H1/00
CPC分类号： C07H21/00 , C07C315/04 , C07C317/36 , C07H19/10 , C07H19/16 , C07H19/20 , Y02P20/55
摘要： Dansylethoxycarbonyl (Dans-EOC) groups which can be employed as base-labile 5'hydroxyl protective groups in chemical oligonucleotide synthesis are disclosed. These groups allow the preparation of a compound of the formula (I) ##STR1## which comprises reacting the compound of the formula (II) ##STR2## with a chlorocarbonyl donor.
摘要翻译： 公开了可用作化学寡核苷酸合成中的碱不稳定的5'-羟基保护基的Dansylethoxycarbonyl（Dans-EOC）基团。 这些基团允许制备式（I）化合物（I），其包括使式（II）化合物与式
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公开(公告)号：US08350038B2
公开(公告)日：2013-01-08
申请号：US12898107
申请日：2010-10-05
申请人： Rainer Beckert , Frank Bergmann , Dieter Heindl , Rupert Herrmann , Hans-Peter Josel , Thomas Welzel
发明人： Rainer Beckert , Frank Bergmann , Dieter Heindl , Rupert Herrmann , Hans-Peter Josel , Thomas Welzel
IPC分类号： C07D217/22 , C07D217/00
CPC分类号： C07D401/04
摘要： Disclosed are pyridinyl-isoquinoline-dione derivatives, methods of producing these derivatives, conjugates comprising the pyridinyl-isoquinoline dione derivatives and (i) a solid support, or (ii) a biomolecule, methods of producing these conjugates as well as the use of these conjugates as quenchers in fluorescence resonance energy transfer (FRET).
摘要翻译： 公开了吡啶基 - 异喹啉二酮衍生物，制备这些衍生物的方法，包含吡啶基 - 异喹啉二酮衍生物的共轭物和（i）固体支持物，或（ii）生物分子，制备这些缀合物的方法以及这些 共轭物作为荧光共振能量转移（FRET）中的猝灭剂。
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公开(公告)号：US20090208930A1
公开(公告)日：2009-08-20
申请号：US11658569
申请日：2005-09-02
CPC分类号： C12Q1/6848 , C12Q1/6858 , C12Q2523/125 , C12Q2521/331
摘要： The invention is related to a method for amplifying a methylated target nucleic acid in a sample while avoiding amplification of a non-methylated target nucleic acid by inactivating it. This is accomplished by a restriction enzyme digest after bisulfite treatment of the target nucleic acid. The invention is further related to the use of a restriction enzyme to avoid amplification of a non-methylated target nucleic acid while amplifying a methylated target nucleic acid in a sample and kits for performing the methods according to the invention.
摘要翻译： 本发明涉及一种用于扩增样品中的甲基化靶核酸的方法，同时避免通过使其失活来扩增非甲基化靶核酸。 这是通过目标核酸亚硫酸氢盐处理后的限制酶消化完成的。 本发明进一步涉及使用限制酶避免扩增未甲基化的靶核酸同时扩增样品中的甲基化靶核酸和用于实施根据本发明的方法的试剂盒。
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公开(公告)号：US20060058518A1
公开(公告)日：2006-03-16
申请号：US10540406
申请日：2004-01-28
CPC分类号： C07H21/04 , C01D5/145 , C12Q1/6827 , C12Q2527/101 , C12Q2523/125
摘要： The present application is directed to a method for performing a bisulfite reaction to determine methylation positions in a nucleic acid, i.e. methylated and non-methylated cytosines, whereby the nucleic acid is incubated in a solution comprising the nucleic acid for a time period of 1.5 to 3.5 hours at a temperature between 70 and 90° C., whereby the concentration of bisulfite in the solution is between 3 M and 6.25 M and whereby the pH value of the solution is between 5.0 and 6.0 whereby the nucleic acid, i.e. the cytosine bases in the nucleic acid, are deaminated. Then the solution comprising the deaminated nucleic acid is desulfonated and preferably desalted. The application is further related to a solution comprising bisulfite with a certain pH and uses thereof as well as a kit comprising the solution.
摘要翻译： 本申请涉及用于进行亚硫酸氢盐反应以确定核酸（即甲基化和非甲基化胞嘧啶）中的甲基化位置的方法，由此将核酸在包含核酸的溶液中温育1.5天至 在70〜90℃的温度下3.5小时，由此在该溶液中的亚硫酸氢盐浓度在3〜6.25M之间，由此溶液的pH值在5.0和6.0之间，由此核酸，即胞嘧啶碱基 在核酸中进行脱氨。 然后将包含脱氨酸核酸的溶液脱磺化并优选脱盐。 该应用还涉及包含具有一定pH的亚硫酸氢盐和其用途的溶液以及包含该溶液的试剂盒。
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公开(公告)号：US06235476B1
公开(公告)日：2001-05-22
申请号：US09242536
申请日：1999-03-17
申请人： Frank Bergmann , Rupert Herrmann , Uwe Kobold
发明人： Frank Bergmann , Rupert Herrmann , Uwe Kobold
IPC分类号： C12Q168
CPC分类号： C12Q1/6872 , C12Q1/6827 , Y10T436/143333
摘要： A method for detecting nucleic acids by binding a probe P to a partial sequence S contained in the nucleic acid to produce a binding product B1, degrading the nucleic acid to produce a binding product B2 containing a partial nucleic acid F of a defined length and detecting the binding product B2 or the partial nucleic acid F a part based on its mass is particularly suitable for the parallel detection of nucleic acid of different sequences.
摘要翻译： 通过将探针P与核酸中所含的部分序列S结合以产生结合产物B1来检测核酸的方法，使核酸降解以产生含有限定长度的部分核酸F的结合产物B2并检测 结合产物B2或部分核酸F基于其质量的部分特别适用于不同序列的核酸的平行检测。
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公开(公告)号：US5962540A
公开(公告)日：1999-10-05
申请号：US776728
申请日：1997-03-03
申请人： Carsten Friese , Frank Bergmann , Thomas Huver
发明人： Carsten Friese , Frank Bergmann , Thomas Huver
IPC分类号： C09J9/00 , C08G18/67 , C08J9/10 , C08L75/16 , C09J4/00 , C09J4/02 , C09J5/08 , C09J11/06 , C09J133/04 , C09J175/00 , C09J175/16 , C09J201/00 , H01F27/32 , C08J9/02
CPC分类号： C08J9/102 , C08G18/672 , C09J175/16 , C09J4/00 , C09J5/08 , C08J2207/02 , C08J2375/16 , C08L75/16 , C09J2475/00
摘要： A reaction adhesive becomes porous after hardening. This allows both small and larger gaps to be sealed between the bonded substrates, as the liquid adhesive has a low to medium viscosity and the hardened glue shows a marked volume increase. Reaction adhesives based on (meth)acrylates and that contain azo-bis-isobutyronitrile as initiator and expanding agent are preferably used. The disclosed reaction adhesive is preferably used for gluing reactive coils.
摘要翻译： PCT No.PCT / EP95 / 02961 Sec。 371日期1997年3月3日 102（e）1997年3月3日PCT PCT 1995年7月25日PCT公布。 公开号WO96 / 04347 日期1996年2月15日硬化后反应粘合剂变多孔。 这允许在粘合的基底之间密封小的和较大的间隙，因为液体粘合剂具有低至中等的粘度，并且硬化的胶显示出显着的体积增加。 优选使用基于（甲基）丙烯酸酯并含有偶氮双异丁腈作为引发剂和发泡剂的反应粘合剂。 所公开的反应粘合剂优选用于胶合反应性线圈。
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公开(公告)号：US08877464B2
公开(公告)日：2014-11-04
申请号：US13191700
申请日：2011-07-27
CPC分类号： C12Q1/686 , C12P19/34 , C12Q1/68 , C12Q1/6851 , G01N21/6428 , G01N2021/6432 , G01N2021/6441
摘要： The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction. Further provided are kits comprising components for performing said methods and uses.
摘要翻译： 本发明涉及使用至少第一对照核酸或不同浓度的第一和第二对照核酸定性和定量检测微生物核酸的新方法和用途。 该方法基于核酸的扩增，例如聚合酶链反应。 还提供了包括用于执行所述方法和用途的组件的套件。
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公开(公告)号：US08445206B2
公开(公告)日：2013-05-21
申请号：US13361549
申请日：2012-01-30
CPC分类号： C12Q1/6841 , C12Q1/6876 , C12Q1/6886 , C12Q2565/1025 , C12Q2563/107
摘要： The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.
摘要翻译： 本公开内容涉及针对感兴趣的基因组靶序列的（或互补）部分的一组至少100个单链寡核苷酸探针。 本公开还涉及使用该组寡核苷酸探针检测感兴趣的基因组靶序列的方法和产生寡核苷酸探针组的方法。 此外，本公开涉及包含该组寡核苷酸探针和至少一种另外的组分的试剂盒。
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