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    WAFER LEVEL SEQUENCING FLOW CELL FABRICATION
    3.
    发明申请
    WAFER LEVEL SEQUENCING FLOW CELL FABRICATION 审中-公开

    公开(公告)号：US20190088463A1

    公开(公告)日：2019-03-21

    申请号：US16128120

    申请日：2018-09-11

    申请人： Complete Genomics, Inc.

    发明人： Shifeng Li ,  Jian Gong ,  Yan-You Lin ,  Cheng Frank Zhong

    IPC分类号： H01L21/02 ,  H01L21/28 ,  H01L29/20

    摘要： A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

    Apparatus for high throughput sequencing of nucleic acids
    4.
    发明授权
    Apparatus for high throughput sequencing of nucleic acids 有权
    用于核酸的高通量测序的装置

    公开(公告)号：US09382585B2

    公开(公告)日：2016-07-05

    申请号：US12261548

    申请日：2008-10-30

    申请人： Arnold Oliphant

    发明人： Arnold Oliphant

    IPC分类号： C12Q1/68 ,  G01N35/00 ,  G01N21/64

    CPC分类号： C12Q1/6874 ,  G01N21/6486 ,  G01N35/0099 ,  G01N2035/00158 ,  G01N2201/0461

    摘要： A scalable reaction and detection system for automated high throughput sequencing of nucleic acids involving a combination of chemical processes and observation processes independent of the chemistry processes. Discrete functional units may be configured in a manner that allows the system to interchangeably utilize different sequencing reaction components in conjunction with discrete apparatus components for optical image collection and/or analysis.

    摘要翻译： 一种可扩展的反应和检测系统，用于核酸的自动化高通量测序，涉及独立于化学过程的化学过程和观察过程的组合。 离散功能单元可以以允许系统与用于光学图像收集和/或分析的分立装置组件可互换地利用不同排序反应组分的方式来配置。

    Methods and compositions for large-scale analysis of nucleic acids using DNA deletions
    5.
    发明授权
    Methods and compositions for large-scale analysis of nucleic acids using DNA deletions 有权
    使用DNA缺失大规模分析核酸的方法和组合物

    公开(公告)号：US09334490B2

    公开(公告)日：2016-05-10

    申请号：US11938096

    申请日：2007-11-09

    申请人： Radoje T. Drmanac

    发明人： Radoje T. Drmanac

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12N15/66

    CPC分类号： C12N15/10 ,  C12N15/66

    摘要： The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are constructs that include pairs of target sequences which are separated by a known distance in the polynucleotide from which they are derived.

    摘要翻译： 本发明一般涉及多核苷酸的分析，特别是衍生自基因组DNA的多核苷酸。 本发明提供了用于这种分析的方法，组合物和系统。 本发明所涵盖的是构建物，其包括在衍生自它们的多核苷酸中被分离已知距离的靶序列对。

    cDNA library for nucleic acid sequencing
    8.
    发明授权
    cDNA library for nucleic acid sequencing 有权
    用于核酸测序的cDNA文库

    公开(公告)号：US09023769B2

    公开(公告)日：2015-05-05

    申请号：US12956802

    申请日：2010-11-30

    申请人： Radoje Drmanac ,  Fredrik Dahl ,  Evan Hurowitz ,  Fredrie Dahl

    发明人： Radoje Drmanac ,  Fredrik Dahl ,  Evan Hurowitz ,  Fredrie Dahl

    IPC分类号： C40B50/06 ,  C12Q1/68 ,  C12N15/10 ,  C40B20/00

    CPC分类号： C12Q1/6869 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12N15/10 ,  C12Q1/6853 ,  C40B20/00

    摘要： The present invention is directed to compositions and methods for nucleic acid identification and detection. Compositions and methods of the present invention include extracting and fragmenting target nucleic acids from a sample, using the fragmented target nucleic acids to produce target nucleic acid templates and subjecting those target nucleic acid templates to amplification methods to form nucleic acid nanoballs. The invention also includes methods of detecting and identifying sequences using various sequencing applications, including sequencing by ligation methods.

    摘要翻译： 本发明涉及用于核酸鉴定和检测的组合物和方法。 本发明的组合物和方法包括从样品中提取和分离靶核酸，使用片段化的靶核酸产生靶核酸模板，并使这些靶核酸模板进行扩增方法以形成核酸纳米棒。 本发明还包括使用各种测序应用检测和鉴定序列的方法，包括通过连接方法测序。

    METHODS FOR ESTIMATING GENOME-WIDE COPY NUMBER VARIATIONS
    10.
    发明申请
    METHODS FOR ESTIMATING GENOME-WIDE COPY NUMBER VARIATIONS 审中-公开
    估计基因型复制数变化的方法

    公开(公告)号：US20140229117A1

    公开(公告)日：2014-08-14

    申请号：US14253642

    申请日：2014-04-15

    申请人： COMPLETE GENOMICS, INC.

    发明人： Aaron Halpern ,  Krishna Pant

    IPC分类号： G06F19/18

    CPC分类号： G16B20/00 ,  C12Q1/6809 ,  C12Q1/6827 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  C12Q2537/16 ,  C12Q2537/165

    摘要： Methods for determining the copy number of a genomic region at a detection position of a target sequence in a sample are disclosed. Genomic regions of a target sequence in a sample are sequenced and measurement data for sequence coverage is obtained. Sequence coverage bias is corrected and may be normalized against a baseline sample. Hidden Markov Model (HMM) segmentation, scoring, and output are performed, and in some embodiments population-based no-calling and identification of low-confidence regions may also be performed. A total copy number value and region-specific copy number value for a plurality of regions are then estimated.

    摘要翻译： 公开了用于确定样品中靶序列的检测位置处的基因组区域的拷贝数的方法。 对样品中靶序列的基因组区域进行测序，并获得序列覆盖度的测量数据。 校正序列覆盖偏差并且可以相对于基线样本进行归一化。 执行隐马尔科夫模型（HMM）分割，评分和输出，并且在一些实施例中，也可以执行基于群体的无呼叫和低置信区域的识别。 然后估计多个区域的总拷贝数值和区域特定拷贝数值。