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公开(公告)号：US12044675B2
公开(公告)日：2024-07-23
申请号：US17483800
申请日：2021-09-23
发明人： Loren L. Looger , Yan Zhang , Eric R. Schreiter , Jeremy P Hasseman , Ilya Kolb
CPC分类号： G01N33/5041 , A61B5/0071 , C12N15/62 , C12N15/85 , A61B2503/40 , C12N2800/107
摘要： Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided.
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公开(公告)号：US11958976B2
公开(公告)日：2024-04-16
申请号：US17975310
申请日：2022-10-27
发明人： Luke D. Lavis , Jonathan B. Grimm
IPC分类号： C07D405/12 , C07D311/80 , C07D313/14 , C07D473/32 , C07D493/10 , C07F7/08 , C09B11/24 , C09B11/28 , G01N21/64
CPC分类号： C09B11/24 , C07D311/80 , C07D313/14 , C07D405/12 , C07D473/32 , C07D493/10 , C07F7/0816 , C09B11/28 , G01N21/6458 , G01N2021/6439
摘要： Provided are a photoactive fluorophore, a photoactive ligand, and a photoactive complex. The photoactive fluorophore includes a photoactivatable derivative of an azetidine-containing Janelia-Fluor dye. The photoactive ligand includes a photoactive fluorophore and a protein tag. The photoactive complex includes a photoactive ligand conjugated to a protein. Also provided are methods of in vivo labeling with and photoactivation of the photoactive fluorophore, ligand, and complex.
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公开(公告)号：US11939355B2
公开(公告)日：2024-03-26
申请号：US17209336
申请日：2021-03-23
发明人： Joshua Dudman , Adam Hantman , Bum-Yeol Hwang , Alla Karpova , Loren Looger , Kimberly Ritola , David Schaffer , Dougal Gowanlock Robinson Tervo , Sarada Viswanathan
IPC分类号： C12N7/00 , C07K14/005 , C07K14/015 , C12N15/86 , C40B30/06 , C40B40/08 , A61K48/00
CPC分类号： C07K14/015 , C07K14/005 , C12N15/86 , C40B30/06 , C40B40/08 , A61K48/00 , C12N2750/14122 , C12N2750/14145 , C12N2810/6027
摘要： The present disclosure provides AAV variants that exhibit a preference for retrograde movement in neurons and methods of using such variants.
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公开(公告)号：US20240052169A1
公开(公告)日：2024-02-15
申请号：US18445510
申请日：2023-09-11
发明人： Luke Lavis , Jonathan B. Grimm
CPC分类号： C09B11/24 , C07B59/002 , C09B1/00 , C09B57/001 , C09B6/00 , C09B47/04 , C09B3/14
摘要： The present invention is generally directed to the synthesis and use of fluorophores. It is more specifically directed to the synthesis and use of deuterated fluorophores. In one case, the present invention provides a compound of the structure shown in FIG. 44.
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公开(公告)号：US11708397B2
公开(公告)日：2023-07-25
申请号：US16768153
申请日：2018-12-31
CPC分类号： C07K14/4728 , A61K49/0004 , A61K49/0041 , A61K49/0056 , G01N21/77 , G01N33/582 , G01N33/84 , C07K2319/20 , C07K2319/60 , G01N2021/7786 , G01N2333/4727
摘要： A chemigenetic calcium indicator and a method of measuring calcium are provided. The chemigenetic calcium indicator includes a calcium-binding protein domain attached to a ligand binding protein domain. The method of measuring calcium includes administering a chemigenetic calcium indicator to a subject and determining changes in fluorescence, the chemigenetic calcium indicator including a ligand binding protein domain having a calcium-binding protein domain and a dye-ligand conjugate attached thereto.
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公开(公告)号：US20230203369A1
公开(公告)日：2023-06-29
申请号：US17797181
申请日：2021-02-12
申请人： NORTHWESTERN UNIVERSITY , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , HOWARD HUGHES MEDICAL INSTITUTE
发明人： Nathan C. GIANNESCHI , Nanzhi ZANG , Massimo SCANZIANI , Dante S. BORTONE , Treffly B. DITRI , Anthony M. RUSH
CPC分类号： C09K11/06 , G01N33/587 , B82Y5/00 , B82Y30/00 , C09K2211/1466 , C09K2211/145
摘要： Disclosed is composition comprising: a first fluorophore moiety, and nanoparticles, wherein the nanoparticles comprise: a first plurality of a first nanoparticle, the first nanoparticle comprising: a first outer surface, a first interior bulk, and a first polymer, wherein the first polymer is covalently bonded to the first fluorophore moiety within the first interior bulk of the first nanoparticle. Also disclosed is a composition comprising: a chelate moiety, and nanoparticles, wherein the nanoparticles comprise: a plurality of a chelate nanoparticle, the chelate nanoparticle comprising: an outer surface, an interior bulk, and a polymer, wherein the polymer is covalently bonded to the chelate moiety within the interior bulk of the chelate nanoparticle. Also disclosed are methods of making such compositions and using such composition for brain mapping and tracing of axonal projections.
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公开(公告)号：US20230095090A1
公开(公告)日：2023-03-30
申请号：US17975310
申请日：2022-10-27
发明人： Luke D. Lavis , Jonathan B. Grimm
IPC分类号： C09B11/24 , G01N21/64 , C07D311/80 , C07F7/08 , C07D493/10 , C07D313/14 , C09B11/28 , C07D405/12 , C07D473/32
摘要： Provided are a photoactive fluorophore, a photoactive ligand, and a photoactive complex. The photoactive fluorophore includes a photoactivatable derivative of an azetidine-containing Janelia-Fluor dye. The photoactive ligand includes a photoactive fluorophore and a protein tag. The photoactive complex includes a photoactive ligand conjugated to a protein. Also provided are methods of in vivo labeling with and photoactivation of the photoactive fluorophore, ligand, and complex.
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公开(公告)号：US20230038702A1
公开(公告)日：2023-02-09
申请号：US17940199
申请日：2022-09-08
发明人： Luke D. LAVIS , Claire DEO
IPC分类号： C07F7/08 , G01N21/78 , C07D405/14 , C07D405/04
摘要： The presently-disclosed subject matter includes fluorescent indicators, including bright and targetable red Ca2+ indicators. The presently-disclosed subject matter also includes kits comprising the same as well as methods for using the same to detect a target substance.
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公开(公告)号：US20220236550A1
公开(公告)日：2022-07-28
申请号：US17711128
申请日：2022-04-01
摘要： A sample is imaged using light-sheet imaging. The light-sheet imaging includes generating light, forming one or more light sheets from the light at one or more positions within the sample along respective illumination directions that are parallel with an illumination axis, and recording images of fluorescence emitted along a detection direction from the sample due to the optical interaction between the one or more light sheets and the sample. One or more properties relating to the light-sheet imaging are measured; the one or more measured properties are analyzed; and one or more operating parameters associated with the light-sheet imaging are adjusted based on the analysis of the one or more measured properties.
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公开(公告)号：US11385236B2
公开(公告)日：2022-07-12
申请号：US16347765
申请日：2017-11-30
IPC分类号： G01N33/58 , C07K14/195 , C07K14/215 , C07K14/435 , C07K14/46 , C07K14/465 , C12N9/14 , G01N21/64 , G01N33/566
摘要： Provided herein are a voltage indicator and a method of measuring voltage. The voltage indicator includes a membrane-localized voltage-sensitive protein coupled to a capture protein. The method of measuring voltage includes administering a voltage indicator including a membrane-localized voltage-sensitive protein coupled to a capture protein, and determining changes in fluorescence of a small-molecule fluorescent dye captured by the capture protein.
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