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    Focused acoustic ejection cell sorting system and method
    3.
    发明申请
    Focused acoustic ejection cell sorting system and method 有权
    聚焦声弹出细胞分选系统及方法

    公开(公告)号：US20050130257A1

    公开(公告)日：2005-06-16

    申请号：US11049206

    申请日：2005-02-01

    申请人： Mitchell Mutz ,  Richard Ellson ,  David Lee

    发明人： Mitchell Mutz ,  Richard Ellson ,  David Lee

    IPC分类号： B41J2/14 ,  C12M1/26 ,  C12M3/00 ,  C12N5/02 ,  G01N15/10 ,  G01N15/14 ,  G01N30/02 ,  C12P33/02 ,  B07C5/02

    CPC分类号： G01N35/1074 ,  B41J2/14008 ,  C12M47/04 ,  G01N15/1056 ,  G01N29/0681 ,  G01N30/02 ,  G01N2015/1081 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2035/1039 ,  B01D2015/389

    摘要： A method is provided for acoustically ejecting from a channel or other container a plurality of fluid droplets, each of which contains one or more particles or other localized volumes. The localized volumes, which can be living cells, are ejected towards sites on a substrate surface, a container, or a channel. An integrated cell sorting and arraying system is also provided that is capable of sorting based upon cellular properties by the selective ejection of cells from a carrier fluid. The cells can be ejected with adjustable velocity and trajectory. The ejected cells can be directed to form an array, wherein each site of the array can contain a single cell. Additionally provided is a method of forming arrays of single live cells more efficiently, rapidly, flexibly, and economically than by other cell array approaches. This method permits efficient, continuous, and simultaneous sorting of cells based upon the quantitative or semiquantitative measurement of cellular properties, and also permits non-binary or severally-branched decision-making. An integrated system, which includes a processor, and methods are also provided for the detection, selection, and ejection of selected particles or circumscribed volumes, such as live cells, from a continuous stream of fluid-suspended particles or other circumscribed volumes flowing in channels.

    摘要翻译： 提供了一种用于从通道或其它容器声学喷射多个流体液滴的液滴，每个流体液滴包含一个或多个颗粒或其它局部体积。 可以是活细胞的局部体积被排出到基底表面，容器或通道上的位置。 还提供了一种综合的细胞分选和排列系统，其能够通过从载体液体中选择性地排出细胞而基于细胞特性进行分选。 可以以可调节的速度和轨迹弹出细胞。 排出的细胞可以被引导以形成阵列，其中阵列的每个位点可以包含单个细胞。 另外提供了一种形成单个活细胞阵列的方法，其比单体阵列方法更有效，快速，灵活和经济地形成。 该方法允许基于细胞性质的定量或半定量测量来对细胞进行有效，连续和同时的分选，并且还允许非二元或分支分支的决策。 还提供了一种包括处理器和方法的集成系统，用于从流体悬浮颗粒的连续流或在通道中流动的其它外接体积检测，选择和排出所选择的颗粒或外切体积（例如活细胞） 。

    Acoustic ejection into small openings
    5.
    发明授权
    Acoustic ejection into small openings 有权
    声音喷射成小开口

    公开(公告)号：US07405395B2

    公开(公告)日：2008-07-29

    申请号：US11041999

    申请日：2005-01-24

    申请人： Richard N. Ellson ,  Mitchell W. Mutz

    发明人： Richard N. Ellson ,  Mitchell W. Mutz

    IPC分类号： H01J49/04 ,  G01N1/10 ,  G01N35/10

    CPC分类号： H01J49/0418 ,  G01N33/54393 ,  G01N35/1011 ,  H01J49/0004 ,  H01J49/0454 ,  Y10T436/2575

    摘要： Provided is a method of transporting fluid which involves acoustic ejection into a small opening. The opening may be, for example, the inlet opening of a sample vessel. Alternatively, it may be an opening in a microfluidic device. The ejection is typically, but not necessarily carried out through the application of focused acoustic energy. The fluid being transported typically comprises a moiety of interest for analysis or further processing, which may be a biomolecule. The volumes of transported fluid may be in the nanoliter or picoliter range. Ejection may occur from very small volumes and the ejected droplet may carry away a significant fraction of the volume from which ejection occurs.

    摘要翻译： 提供了将声弹出的液体输送到小开口中的方法。 开口可以是例如样品容器的入口。 或者，其可以是微流体装置中的开口。 喷射通常但不一定通过应用聚焦声能来进行。 正在运输的流体通常包含用于分析或进一步加工的部分，其可以是生物分子。 运输流体的体积可以在纳升或皮升范围内。 喷射可以从非常小的体积发生，并且喷射的液滴可能携带排出发生的体积的很大一部分。

    Methods, devices, and systems using acoustic ejection for depositing fluid droplets on a sample surface for analysis
    8.
    发明授权
    Methods, devices, and systems using acoustic ejection for depositing fluid droplets on a sample surface for analysis 失效
    用于在样品表面上沉积流体液滴进行分析的声喷射的方法，装置和系统

    公开(公告)号：US06855925B2

    公开(公告)日：2005-02-15

    申请号：US10379379

    申请日：2003-03-03

    申请人： Richard N. Ellson ,  Mitchell W. Mutz ,  Richard Michael Caprioli

    发明人： Richard N. Ellson ,  Mitchell W. Mutz ,  Richard Michael Caprioli

    IPC分类号： G01N33/543 ,  G01N35/10 ,  H01J49/04 ,  G01N1/10

    CPC分类号： H01J49/0418 ,  G01N33/54393 ,  G01N35/1011 ,  H01J49/0004 ,  H01J49/0454 ,  Y10T436/2575

    摘要： Provided is a method for preparing a sample surface for analysis that involves placing a sample surface in droplet-receiving relationship to a reservoir containing an analysis-enhancing fluid. Typically, the analysis-enhancing fluid is comprised of a mass spectrometry matrix material and a carrier fluid, and the carrier fluid is comprised of a low volatility solvent. A droplet of the analysis-enhancing fluid from the reservoir such that the droplet is deposited on the sample surface at a designated site. Such ejection is typically, but not necessarily carried out through the application of focused acoustic energy. Then, the sample is subjected to conditions sufficient to allow the analysis-enhancing fluid to interact with the sample surface to render the sample surface suitable for analysis. Optionally, the sample is analyzed at the selected site. Also provided are systems and devices for preparing a sample surface for analysis.

    摘要翻译： 提供了一种用于制备用于分析的样品表面的方法，其涉及将样品表面与包含分析增强流体的储液器的液滴接收关系放置。 通常，分析增强流体由质谱法基质材料和载体流体组成，并且载体流体由低挥发性溶剂组成。 来自储存器的分析增强流体的液滴使得液滴在指定位置沉积在样品表面上。 这种喷射通常但不一定通过应用聚焦声能来进行。 然后，样品经受足以使分析增强流体与样品表面相互作用以使样品表面适合分析的条件。 任选地，在所选择的位点分析样品。 还提供了用于制备用于分析的样品表面的系统和装置。

    Droplet dispensation from a reservoir with reduction in uncontrolled electrostatic charge
    9.
    发明申请
    Droplet dispensation from a reservoir with reduction in uncontrolled electrostatic charge 有权
    来自储存器的液滴分配，减少不受控制的静电电荷

    公开(公告)号：US20070153049A1

    公开(公告)日：2007-07-05

    申请号：US11714391

    申请日：2007-03-05

    申请人： Mitchell Mutz ,  David Lee ,  George McLendon

    发明人： Mitchell Mutz ,  David Lee ,  George McLendon

    IPC分类号： B41J2/015

    CPC分类号： B41J2/04511 ,  B41J2/04526 ,  B41J2/04575 ,  B41J2/14008

    摘要： Devices and methods are provided for reducing the uncontrolled electrostatic charges that can alter the volume and/or trajectory of a droplet, which is typically ejected through the application of focused acoustic radiation. Also provided are reservoirs and substrates, e.g., well plates formed from a material that is at least partially nonmetallic or polymeric and either has an electrical resistivity of no more than about 1011 ohm-cm, has a surface electrical resistivity of no more than about 1012 ohm/sq, or both.

    摘要翻译： 提供了用于减少不受控制的静电电荷的装置和方法，所述静电电荷可以改变液滴的体积和/或轨迹，所述液滴和/或轨迹通常通过聚焦声辐射的应用而被喷射。 还提供了储存器和基底，例如，由至少部分非金属或聚合物材料形成的并且具有不大于约10欧姆 - 厘米的电阻率的材料形成的孔板具有表面 电阻率不超过约10欧姆/平方或两者。