Abstract:
Provided are GH10 xylanases or a fragment thereof including modified xlyanase enzymes, all having xylanase activity, wherein the enzyme or fragment thereof provides increased oil recovery from a grain-based material compared with a control or parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at at least one or more of the following positions 7, 25, 33, 64, 79, 89, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). Methods of using the GH10 xylanases, for example, for improved oil recovery are also provided.
Abstract translation:提供的GH10木聚糖酶或其片段包括全部具有木聚糖酶活性的改性的xlyanase酶,其中与对照或亲本GH10木聚糖酶相比,其酶或片段提供了从谷物基材料提高的油回收率,母本GH10木聚糖酶已被 在以下至少一个或多个位置7,25,33,64,79,89,217和298处修饰,其中所述编号基于FveXyn4(SEQ ID No.1)的氨基酸编号。 还提供了使用GH10木聚糖酶的方法,例如用于改进的油回收。
Abstract:
The present invention relates to reducing the viscosity of grain slurry and reduction in foaming during liquefaction and/or fermentation using proteases. The invention also relates to increasing the rate and/or yield of fermentation products, including ethanol from grains.
Abstract:
The present compositions and methods relate to a beta-glucosidase from Melanocarpus albomyces, polynucleotides encoding the beta-glucosidase, and methods of making and/or using the same. Formulations containing the beta-glucosidase are suitable for use in numerous applications, including hydrolyzing lignocellulosic biomass substrates.
Abstract:
The present invention relates to methods for improving fermentation processes, including increasing product yield, reduced viscosity, and/or reduced foaming.
Abstract:
The present teachings provide a method for coating particles containing thermally sensitive biologically active materials encapsulated by uniform thin protective moisture barrier coatings, and processes for applying such coatings efficiently, at ambient temperatures and with minimal agglomeration. The coating formulations can comprise dispersants, adhesion promoters, polymers, and plasticizers dissolved or dispersed in a non-flammable, low boiling point solvent such as methylene chloride that is delivered with an aerosol process to target particles. The present teachings also include the resulting particles.
Abstract:
Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
Abstract:
Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
Abstract:
This present invention relates to cultured recombinant cells comprising a heterologous phosphoketolase (PKL) polypeptide that are capable of increased production of acetyl coenzyme A-derived metabolites, as well as methods for producing and using the same. In some embodiments, the recombinant cells further comprise one or more mevalonate (MVA) pathway polypeptides for the production of isoprenoid precursors, isoprene and isoprenoids.
Abstract:
A fungal alpha-amylase is provided from Aspergillus terreus (AtAmy1). AtAmy1 has an optimal pH of 4.5 and is operable at 30 75 degrees C, allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.