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    METHOD AND SYSTEM FOR DETERMINING A BIOLOGICAL RESPONSE OF A TARGET TO A SOLUBLE CANDIDATE SUBSTANCE
    81.
    发明申请
    METHOD AND SYSTEM FOR DETERMINING A BIOLOGICAL RESPONSE OF A TARGET TO A SOLUBLE CANDIDATE SUBSTANCE 审中-公开
    用于确定目标到可溶性候选物质的生物反应的方法和系统

    公开(公告)号：WO2014135512A1

    公开(公告)日：2014-09-12

    申请号：PCT/EP2014/054117

    申请日：2014-03-04

    Applicant: F. HOFFMANN-LA ROCHE AG

    Inventor： WERNER, Michael ,  MARTIN, Rainer E. ,  HOCHSTRASSER, Remo

    IPC: G01N33/536 ,  G01N33/558 ,  B01L3/00

    CPC classification number: G01N33/536 ,  B01L3/502776 ,  B01L2200/0694 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0472 ,  B01L2400/0487 ,  C12Q1/25 ,  G01N21/6428 ,  G01N21/6486 ,  G01N33/558 ,  G01N2021/6439 ,  Y10T436/25625 ,  Y10T436/2575

    Abstract: A method for determining a biological response of a target (41, 42) to a soluble candidate substance comprises the steps: introducing a soluble candidate substance into a laminar flow of a buffer liquid (2) to form a candidate substance solute (3) having an initial concentration profile (31); dispersing the initial concentration profile (31) to form a dispersed concentration profile (32); directing the dispersed concentration profile (32) into a detection channel (12) to form a final symmetrical concentration profile (33) therein; introducing a target into the detection channel (12) to obtain a combined concentration profile comprising a constant target concentration profile overlying the final symmetrical concentration profile (33); holding in the detection channel (12) at least one half of the combined concentration profile; and optically scanning the combined concentration profile to detect an optical signal representative of the biological response of the target to the soluble candidate substance.

    Abstract translation: 用于确定靶（41,42）对可溶性候选物质的生物反应的方法包括以下步骤：将可溶性候选物质引入缓冲液体（2）的层流中以形成候选物质溶质（3），其具有 初始浓度分布（31）; 分散初始浓度分布（31）以形成分散浓度分布（32）; 将分散的浓度分布（32）引导到检测通道（12）中以在其中形成最终对称的浓度分布（33）; 将目标引入检测通道（12）以获得组合浓度分布，其包括覆盖最终对称浓度分布（33）的恒定目标浓度分布; 在所述检测通道（12）中保持所述组合浓度分布的至少一半; 并且光学扫描组合的浓度分布，以检测表示靶向可溶性候选物质的生物反应的光学信号。

    COMPOSITIONS, METHODS AND MICROFLUIDICS DEVICE FOR TELOMERASE BASED IN VITRO DIAGNOSTIC ASSAYS FOR DETECTING CIRCULATING TUMOR CELLS (CTC)
    84.
    发明申请
    COMPOSITIONS, METHODS AND MICROFLUIDICS DEVICE FOR TELOMERASE BASED IN VITRO DIAGNOSTIC ASSAYS FOR DETECTING CIRCULATING TUMOR CELLS (CTC) 审中-公开
    用于检测循环肿瘤细胞（CTC）的体外诊断测定的TELOMERASE的组合物，方法和微流感装置

    公开(公告)号：WO2014065861A1

    公开(公告)日：2014-05-01

    申请号：PCT/US2013/031698

    申请日：2013-03-14

    Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA

    Inventor： HAHN, Stephen, M. ,  DORSEY, Jay, F. ,  KAO, Gary, D. ,  REYES, Emigdio

    IPC: A61K35/76

    CPC classification number: G01N33/574 ,  B01L3/502753 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/086 ,  C12Q1/6897 ,  G01N33/54366 ,  G01N2030/524 ,  G01N30/6095 ,  G01N30/6069

    Abstract: A repeatable method for detecting circulating tumor cells in vitro is provided. The method involves combining a test sample from a patient suspected of having circulating tumor cells, and a non-lytic adenoviral system, and culture media for the cells. The adenoviral system utilizes (i) a first replication-defective adenoviral particle in which an expression cassette is packaged, said expression cassette comprising an adenoviral 5' and 3' ITRs and a tumor-specific promoter; and (ii) a coding sequence for a Teporter protein which is expressed in the presence of circulating tumor cells, and an adenoviral 3' ITR. The test sample and the non-lytic adenoviral system are incubated for a sufficient time to permit expression of the reporter protein, and measuring reporter protein expression in the test samples, whereby presence of reporter expression indicates the presence of circulating tumor cells in the sample.

    Abstract translation: 提供了一种可重复检测体外循环肿瘤细胞的方法。 该方法包括将来自怀疑患有循环肿瘤细胞的患者的测试样品和非溶解性腺病毒系统以及用于细胞的培养基组合。 腺病毒系统利用（i）其中包装表达盒的第一复制缺陷型腺病毒颗粒，所述表达盒包含腺病毒5'和3'ITR和肿瘤特异性启动子; 和（ii）在循环肿瘤细胞存在下表达的Teporter蛋白的编码序列和腺病毒3'ITR。 将测试样品和非溶解性腺病毒系统孵育足够的时间以允许报告蛋白的表达，并测量测试样品中的报道蛋白表达，由此报告基因表达的存在表明样品中存在循环肿瘤细胞。

    DROPLET GENERATION SYSTEM WITH FEATURES FOR SAMPLE POSITIONING
    87.
    发明申请
    DROPLET GENERATION SYSTEM WITH FEATURES FOR SAMPLE POSITIONING 审中-公开
    具有样本定位特征的DROPLET生成系统

    公开(公告)号：WO2014018562A1

    公开(公告)日：2014-01-30

    申请号：PCT/US2013/051735

    申请日：2013-07-23

    Applicant: BIO-RAD LABORATORIES, INC.

    Inventor： CAULEY, Thomas, H., III ,  BRACKBILL, Timothy ,  BOUSSE, Luc ,  PETERSEN, Jon

    IPC: B01F3/08 ,  B01L3/00 ,  B01J19/00

    CPC classification number: G01N1/28 ,  B01F3/0807 ,  B01F13/0062 ,  B01L3/502784 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/084 ,  Y10T436/25

    Abstract: System, including methods and apparatus, for forming droplets of an emulsion. The system may include a channel junction at which a stream of sample fluid is divided into droplets by a dividing flow of carrier fluid. The system also may include one or more features configured to position sample fluid for reduced contact between the sample fluid and one or more surface regions of the channel junction, which may improve the consistency of droplet formation. In exemplary embodiments, sample fluid may be positioned by a step member produced by an increase in channel depth, and/or by directing flow of carrier fluid to form a barrier layer between sample fluid and a wall region, such as a ceiling region or floor region, of a channel network.

    Abstract translation: 用于形成乳液液滴的系统，包括方法和装置。 该系统可以包括通道结，在该通道连接处，样品流体流通过载体流体的分流被分成液滴。 该系统还可以包括被配置为定位样品流体以减少样品流体和通道结的一个或多个表面区域之间的接触的一个或多个特征，这可以提高液滴形成的一致性。 在示例性实施例中，样品流体可以由通过增加通道深度产生的台阶构件定位，和/或通过引导载体流体的流动来形成样品流体和壁区域之间的阻挡层，例如天花板区域或地板 区域，通道网络。

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