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公开(公告)号：US09316331B2
公开(公告)日：2016-04-19
申请号：US14091342
申请日：2013-11-27
Applicant: Fluidigm Corporation
Inventor： Geoffrey Facer , Brian Fowler , Emerson Cheung Quan , Marc Unger
IPC: B01L3/00 , G05D11/02 , F16K99/00 , B01F5/02 , B01F13/00 , C12Q1/68 , G01N21/75 , F16K3/00 , G01N21/03
CPC classification number: F16K99/0015 , B01F5/02 , B01F13/0059 , B01L3/502707 , B01L3/502738 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , B33Y80/00 , C12Q1/686 , F16K99/0026 , F16K99/0059 , F16K2099/008 , F16K2099/0084 , G01N2021/0346 , Y10T137/0329 , Y10T137/2164 , Y10T137/2169 , Y10T137/2559 , Y10T137/2655 , Y10T137/2688 , Y10T137/87249
Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.
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公开(公告)号：US20140357513A1
公开(公告)日：2014-12-04
申请号：US14331112
申请日：2014-07-14
Applicant: Fluidigm Corporation
Inventor： Michael Lucero , Marc Unger
CPC classification number: C12Q1/686 , C12Q1/6804 , C12Q1/6813 , C12Q1/6876 , C12Q2600/118 , G01N33/53 , G01N33/6845 , G01N2333/435 , G01N2458/10
Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.
Abstract translation: 本发明提供了用于检测和/或定量样品中多个核酸或蛋白质靶标的测定方法。 在该方法中，探针用于将可检测标签序列与存在于样品中的每个选定靶标相关联。 使用足以鉴定至少25个，优选至少500个不同靶标的探针或引物。 该方法包括将样品的等分试样彼此分离并检测每个等分试样中的标签序列。
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公开(公告)号：US08616227B1
公开(公告)日：2013-12-31
申请号：US13909000
申请日：2013-06-03
Applicant: Fluidigm Corporation
Inventor： Geoffrey Facer , Brian Fowler , Emerson Cheung Quan , Marc Unger
CPC classification number: F16K99/0015 , B01F5/02 , B01F13/0059 , B01L3/502707 , B01L3/502738 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , B33Y80/00 , C12Q1/686 , F16K99/0026 , F16K99/0059 , F16K2099/008 , F16K2099/0084 , G01N2021/0346 , Y10T137/0329 , Y10T137/2164 , Y10T137/2169 , Y10T137/2559 , Y10T137/2655 , Y10T137/2688 , Y10T137/87249
Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.
Abstract translation: 多级微流体装置包括一个控制管线，可同时为样品和试剂管线起动阀门。 微流体装置被配置为在第二室中的第一室和第二试剂中含有第一试剂，其中第一试剂和第二试剂中的任一种或两者以所需或选择的压力包含。 微流体装置的操作包括将第二试剂从第二室传输到第一室，用于与第一试剂混合或接触。 诸如通道，阀，腔室的微流体装置特征可以至少部分地包含，嵌入或由弹性体块的一个或多个层或层形成或形成。
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公开(公告)号：US20130337457A1
公开(公告)日：2013-12-19
申请号：US13909000
申请日：2013-06-03
Applicant: Fluidigm Corporation
Inventor： Geoffrey Facer , Brian Fowler , Emerson Cheung Quan , Marc Unger
IPC: C12Q1/68
CPC classification number: F16K99/0015 , B01F5/02 , B01F13/0059 , B01L3/502707 , B01L3/502738 , B01L2200/10 , B01L2200/12 , B01L2200/16 , B01L2300/0627 , B01L2300/0816 , B01L2300/0874 , B01L2300/0887 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , B33Y80/00 , C12Q1/686 , F16K99/0026 , F16K99/0059 , F16K2099/008 , F16K2099/0084 , G01N2021/0346 , Y10T137/0329 , Y10T137/2164 , Y10T137/2169 , Y10T137/2559 , Y10T137/2655 , Y10T137/2688 , Y10T137/87249
Abstract: Multilevel microfluidic devices include a control line that can simultaneously actuate valves for both sample and reagent lines. Microfluidic devices are configured to contain a first reagent in a first chamber and a second reagent in a second chamber, where either or both of the first and second reagents are contained at a desired or selected pressure. Operation of a microfluidic device includes transmitting second reagent from the second chamber to the first chamber, for mixing or contact with the first reagent. Microfluidic device features such as channels, valves, chambers, can be at least partially contained, embedded, or formed by or within one or more layers or levels of an elastomeric block.
Abstract translation: 多级微流体装置包括一个控制管线，可同时为样品和试剂管线起动阀门。 微流体装置被配置为在第二室中的第一室和第二试剂中含有第一试剂，其中第一试剂和第二试剂中的任一种或两者以所需或选择的压力包含。 微流体装置的操作包括将第二试剂从第二室传输到第一室，用于与第一试剂混合或接触。 诸如通道，阀，腔室的微流体装置特征可以至少部分地包含，嵌入或由弹性体块的一个或多个层或层形成或形成。
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公开(公告)号：US20190169676A1
公开(公告)日：2019-06-06
申请号：US16150096
申请日：2018-10-02
Applicant: Fluidigm Corporation
Inventor： Marc Unger , Ian D. Manger , Michael Lucero , Yong Yi , Emily Miyashita-Lin , Anja Wienecke , Geoffrey Facer
IPC: C12Q1/68 , B01L3/00 , C12Q1/686 , G01N27/453
Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
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Patent Agency Ranking
公开(公告)号：US20160129441A1
公开(公告)日：2016-05-12
申请号：US14873958
申请日：2015-10-02
Applicant: Fluidigm Corporation
Inventor： Lincoln McBride , Geoffrey Facer , Marc Unger , Michael Lucero , Hany Ramez Nassef
IPC: B01L3/00
CPC classification number: C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
Abstract: An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
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公开(公告)号：US20160091479A1
公开(公告)日：2016-03-31
申请号：US14809972
申请日：2015-07-27
Applicant: Fluidigm Corporation
Inventor： Hany Nassef , Geoffrey Facer , Marc Unger
IPC: G01N33/487 , G01N15/12 , G01N15/10
CPC classification number: C03C25/68 , B01L3/502707 , B01L2300/0645 , B01L2300/0816 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , G01N15/1031 , G01N15/12 , G01N27/00 , G01N27/60 , G01N33/48707 , G01N33/48721 , G01N2015/1087 , Y10T436/2575
Abstract: The presence of a detectable entity within a detection volume of a microfabricated elastomeric structure is sensed through a change in the electrical or magnetic environment of the detection volume. In embodiments utilizing electronic detection, an electric field is applied to the detection volume and a change in impedance, current, or combined impedance and current due to the presence of the detectable entity is measured. In embodiments utilizing magnetic detection, the magnetic properties of a magnetized detected entity alter the magnetic field of the detection volume. This changed magnetic field induces a current which can reveal the detectable entity. The change in resistance of a magnetoresistive element may also reveal the passage of a magnetized detectable entity.
Abstract translation: 通过检测体积的电气或磁环境的变化来检测在微制造弹性体结构的检测体积内的可检测实体的存在。 在利用电子检测的实施例中，对检测体积施加电场，并且测量由于可检测实体的存在引起的阻抗，电流或组合阻抗和电流的变化。 在利用磁检测的实施例中，磁化检测实体的磁性改变了检测体积的磁场。 这种改变的磁场诱发可以揭示可检测实体的电流。 磁阻元件的电阻变化也可以揭示磁化的可检测实体的通过。
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公开(公告)号：US20150159132A1
公开(公告)日：2015-06-11
申请号：US14568597
申请日：2014-12-12
Applicant: Fluidigm Corporation
Inventor： NIANZHEN LI , Marc Unger
CPC classification number: C12N5/0618 , B01L3/5027 , B01L2200/0647 , B01L2300/0816 , B01L2300/0829 , B01L2300/0864 , C12M23/16 , C12N5/0619 , C12N15/111 , C12N2310/141 , C12N2320/12 , C12N2501/65 , C12N2503/02 , C12N2506/02 , C12N2506/1307 , C12Q1/6881 , C12Q2600/158 , G01N33/5026 , G01N33/57438
Abstract: This invention provides technology for transdifferentiating cells from one cell type to another. The cells are cultured with one or more vector-free gene regulator oligonucleotides concurrently or in succession, and then harvested when cell markers or the morphology of the culture shows that transdifferentiation is complete. Suitable gene regular oligonucleotides include microRNAs and messenger RNAs that encode a differentiation factor. Conditions for transdifferentiation can be optimized by dividing cells into different culture chambers of a microfluidic device. Cells are cultured with different additives in each chamber, and then compared. Transdifferentiated cells produced according to this invention can provide a consistent source of tissue for use in regenerative medicine.
Abstract translation: 本发明提供了将细胞从一种细胞类型转移到另一种细胞类型的技术。 细胞与一种或多种无载体的基因调节寡核苷酸同时或相继培养，然后当细胞标记物或培养物的形态显示转分化完成时收获细胞。 合适的基因正常寡核苷酸包括编码分化因子的微小RNA和信使RNA。 可以通过将细胞分成微流体装置的不同培养室来优化用于转分化的条件。 每个室中用不同的添加剂培养细胞，然后进行比较。 根据本发明生产的转分化细胞可提供用于再生医学的一致的组织来源。
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公开(公告)号：US20140296090A1
公开(公告)日：2014-10-02
申请号：US14184499
申请日：2014-02-19
Applicant: FLUIDIGM CORPORATION
Inventor： Alain Mir , Ramesh Ramakrishnan , Marc Unger , Bernhard G. Zimmermann
Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay.
Abstract translation: 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。
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公开(公告)号：US09644235B2
公开(公告)日：2017-05-09
申请号：US14810388
申请日：2015-07-27
Applicant: Fluidigm Corporation
Inventor： Michael Lucero , Marc Unger
CPC classification number: C12Q1/686 , C12Q1/6804 , C12Q1/6813 , C12Q1/6876 , C12Q2600/118 , G01N33/53 , G01N33/6845 , G01N2333/435 , G01N2458/10
Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.
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