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    METHOD FOR FABRICATING A MICROARRAY OF SOFT MATERIALS
    5.
    发明申请
    METHOD FOR FABRICATING A MICROARRAY OF SOFT MATERIALS 有权
    微软材料制作方法

    公开(公告)号：US20130136863A1

    公开(公告)日：2013-05-30

    申请号：US13814588

    申请日：2012-03-27

    申请人： Satoshi Hiyama ,  Kaori Kuribayashi ,  Hiroaki Onoe ,  Shoji Takeuchi

    发明人： Satoshi Hiyama ,  Kaori Kuribayashi ,  Hiroaki Onoe ,  Shoji Takeuchi

    IPC分类号： B05D1/32

    CPC分类号： B05D1/325 ,  B01J2219/00317 ,  B01J2219/00529 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B81B2201/0214 ,  B81B2201/058 ,  B81C1/00206 ,  C12N15/09 ,  G01N33/543 ,  G01N37/00

    摘要： A method for fabricating a microarray of plural soft materials includes: vapor-depositing a first layer poly(para-xylylene) resin on a substrate, forming a first micro pattern in the poly(para-xylylene) resin; obtaining a substrate including a first microarray formed by pouring a first soft material solution, freeze-drying the first soft material to obtain a micro-arrayed substrate of the freeze-dried first soft material; vapor-depositing a second layer poly(para-xylylene) resin on the micro-arrayed substrate of the freeze-dried first soft material, forming a second micro pattern placed differently from the first micro pattern by penetrating the poly(para-xylylene) resin of the first and second layers, forming a second microarray on the substrate by pouring a second soft material solution; and forming a microarray of the first and second soft materials on the substrate by peeling off the poly(para-xylylene) resin of the first and second layers.

    摘要翻译： 一种制备多种软材料的微阵列的方法包括：在基材上蒸镀第一层聚（对二甲苯）树脂，在聚（对二甲苯）树脂中形成第一微图案; 获得包括通过倾倒第一软质材料溶液形成的第一微阵列的基板，冷冻干燥第一软质材料以获得冷冻干燥的第一软质材料的微阵列基板; 在冷冻干燥的第一软质材料的微阵列基底上气相沉积第二层聚（对二甲苯）树脂，通过穿透聚（对二甲苯）树脂形成不同于第一微图案的第二微图案 的第一和第二层，通过倾倒第二软材料溶液在基底上形成第二微阵列; 以及通过剥离第一层和第二层的聚（对二甲苯）树脂，在基材上形成第一和第二软材料的微阵列。

    Method for fabricating a microarray of soft materials
    6.
    发明授权
    Method for fabricating a microarray of soft materials 有权
    软材料微阵列的制造方法

    公开(公告)号：US08999443B2

    公开(公告)日：2015-04-07

    申请号：US13814588

    申请日：2012-03-27

    申请人： Satoshi Hiyama ,  Kaori Kuribayashi ,  Hiroaki Onoe ,  Shoji Takeuchi

    发明人： Satoshi Hiyama ,  Kaori Kuribayashi ,  Hiroaki Onoe ,  Shoji Takeuchi

    IPC分类号： C23C16/00 ,  B05D1/32 ,  C12N15/09 ,  G01N37/00 ,  G01N33/543 ,  B81C1/00

    CPC分类号： B05D1/325 ,  B01J2219/00317 ,  B01J2219/00529 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  B01J2219/00725 ,  B81B2201/0214 ,  B81B2201/058 ,  B81C1/00206 ,  C12N15/09 ,  G01N33/543 ,  G01N37/00

    摘要： A method for fabricating a microarray of plural soft materials includes: vapor-depositing a first layer poly(para-xylylene) resin on a substrate, forming a first micro pattern in the poly(para-xylylene) resin; obtaining a substrate including a first microarray formed by pouring a first soft material solution, freeze-drying the first soft material to obtain a micro-arrayed substrate of the freeze-dried first soft material; vapor-depositing a second layer poly(para-xylylene) resin on the micro-arrayed substrate of the freeze-dried first soft material, forming a second micro pattern placed differently from the first micro pattern by penetrating the poly(para-xylylene) resin of the first and second layers, forming a second microarray on the substrate by pouring a second soft material solution; and forming a microarray of the first and second soft materials on the substrate by peeling off the poly(para-xylylene) resin of the first and second layers.

    摘要翻译： 一种制备多种软材料的微阵列的方法包括：在基材上蒸镀第一层聚（对二甲苯）树脂，在聚（对二甲苯）树脂中形成第一微图案; 获得包括通过倾倒第一软质材料溶液形成的第一微阵列的基板，冷冻干燥第一软质材料以获得冷冻干燥的第一软质材料的微阵列基板; 在冷冻干燥的第一软质材料的微阵列基底上气相沉积第二层聚（对二甲苯）树脂，通过穿透聚（对二甲苯）树脂形成不同于第一微图案的第二微图案 的第一和第二层，通过倾倒第二软材料溶液在基底上形成第二微阵列; 以及通过剥离第一层和第二层的聚（对二甲苯）树脂，在基材上形成第一和第二软材料的微阵列。

    INSTRUMENT AND METHOD FOR TREATING MINOR AMOUNT OF BIOLOGICAL MATERIAL
    7.
    发明申请
    INSTRUMENT AND METHOD FOR TREATING MINOR AMOUNT OF BIOLOGICAL MATERIAL 审中-公开
    用于处理少量生物材料的仪器和方法

    公开(公告)号：US20100279398A1

    公开(公告)日：2010-11-04

    申请号：US12812332

    申请日：2009-01-09

    申请人： Shoji Takeuchi ,  Hiroaki Onoe

    发明人： Shoji Takeuchi ,  Hiroaki Onoe

    IPC分类号： C12N5/00 ,  C12M1/00

    CPC分类号： C12M25/08 ,  B01L3/502761 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2200/0668 ,  B01L2300/0819

    摘要： Provided is a technique for handling a minor amount of a biological material with the purpose of observing selectively the interaction between single cells by controlling cell number and two- or three-dimensional configurations of each of the different cell types. The present invention provides an instrument and a method for handling a minor amount of a biological material. The instrument of the present invention for handling a minor amount of a biological material comprises a construct and a base, wherein: the base holds the construct in a releasable manner; the construct is held on a surface of the base in such a manner that a part of the construct is exposed externally on the outer face of the instrument; and at least a part of a surface of the constructs is exposed externally on the outer face of the instrument being fabricated so as to allow the adhesion of the minor amount of the biological material thereto.

    摘要翻译： 提供了一种用于处理少量生物材料的技术，其目的是通过控制每种不同细胞类型的细胞数量和二维或三维构型来选择性地观察单细胞之间的相互作用。 本发明提供了一种用于处理少量生物材料的仪器和方法。 用于处理少量生物材料的本发明的仪器包括构建体和基底，其中：所述基座以可释放的方式保持构造; 构造体以使得构件的一部分在仪器的外表面外部暴露的方式被保持在基座的表面上; 并且所述构造体的表面的至少一部分在外部暴露在被制造的器械的外表面上，以便允许少量的生物材料粘附到其上。