公开(公告)号：US20250033048A1

公开(公告)日：2025-01-30

申请号：US18713121

申请日：2021-12-15

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Raghuvir N. Sengupta ,  Viktor Shkolnikov ,  Steven Barcelo

IPC: B01L3/00

Abstract: An example microfluidic device comprises a plurality of fluid channels, an unblocking actuator, and a fluid ejection device fluidically coupled to the plurality of fluid channels. The plurality of fluid channels include a plurality of dehydrated reagents disposed within the plurality of fluid channels and a plurality of fluid blocking regions including blocking material disposed between the first fluid chamber and the plurality of dehydrated reagents. The unblocking actuator is coupled to the plurality of fluid channels proximal to the plurality of fluid blocking region.

公开(公告)号：US20240390907A1

公开(公告)日：2024-11-28

申请号：US18202827

申请日：2023-05-26

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: B01L3/00

Abstract: Microfluidic systems are provided for the detection and analysis of cell systems that can include a set of reservoirs, the set of reservoirs including a reagent reservoir and an immiscible fluid reservoir, an incubation region having an inlet and an outlet, a set of independent fluidic networks, the set of independent fluidic networks including a first fluidic network and a second fluidic network that is not fluidically connected to the first fluidic network, wherein the first fluidic network (1) is fed by the set of reservoirs, (2) includes a droplet generator, and (3) connects to the inlet of the incubation region, and wherein the second fluidic network is connected to the outlet of the incubation region.

公开(公告)号：US12115534B2

公开(公告)日：2024-10-15

申请号：US17047359

申请日：2018-07-26

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Daixi Xin ,  Ken Ward

IPC: B01L3/00 ,  G01N35/00

CPC classification number: B01L3/508 ,  B01L3/5085 ,  G01N35/0099 ,  B01L2200/0668 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/12 ,  B01L2400/0424

Abstract: An example system includes a well plate having at least one well, an array of electrodes positioned on a surface of a bottom floor of the well, and a controller to direct electrical voltage to the electrodes to generate a non-uniform electrical field. The electrical field is to direct an object in the electrical field to a target position in the well.

公开(公告)号：US12102998B2

公开(公告)日：2024-10-01

申请号：US17297219

申请日：2019-02-01

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Alexander Govyadinov ,  Viktor Shkolnikov

IPC: B01L3/00 ,  B01F23/00 ,  B01F23/41 ,  B01F101/23 ,  B01L7/00 ,  B23Q17/24 ,  C08J3/075 ,  C08K3/16 ,  C08K3/22 ,  C08K3/32 ,  C12Q1/18 ,  G01N15/01 ,  G01N15/14 ,  G01N15/149 ,  G01N21/64 ,  G01N27/411 ,  G01N33/50

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L7/52 ,  G01N15/1484 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/147 ,  B01L2300/0663 ,  B01L2300/1827 ,  B01L2400/0442 ,  G01N15/01 ,  G01N15/149 ,  G01N2015/1493

Abstract: In one example in accordance with the present disclosure, a cell sorting device is described. The cell sorting device includes a microfluidic channel to serially transport individual cells from a volume of cells along a flow path. A sensor disposed in the microfluidic channel distinguishes between a cell to be analyzed and waste fluid. The cell sorting device includes at least two fluid transport devices disposed within the microfluidic channel. The at least two fluid transport devices include a cell ejector to, responsive to detection of a cell to be analyzed, eject the cell to be analyzed from the cell sorting device and a waste transport device to direct the waste fluid to a waste reservoir.

公开(公告)号：US20240288368A1

公开(公告)日：2024-08-29

申请号：US18564526

申请日：2021-05-28

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Alexander Govyadinov

IPC: G01N21/64 ,  G01N21/03 ,  G01N21/05 ,  G01N33/50

CPC classification number: G01N21/6428 ,  G01N21/0332 ,  G01N21/05 ,  G01N33/5005 ,  G01N2021/0325 ,  G01N2021/052 ,  G01N2201/0636 ,  G01N2201/0638 ,  G01N2201/0806

Abstract: In one example in accordance with the present disclosure, a fluorescence detection system is described. The fluorescence detection system includes a microfluidic chamber to receive a sample containing a compound to be detected. The system also includes a light guide, a portion of which is adjacent the microfluidic chamber. The light guide refracts excitation light into the microfluidic chamber to excite fluorophores in the microfluidic chamber. The light guide has total internal reflection in those portions that are not adjacent the microfluidic chamber. The fluorescence detection system also includes a heating element to trigger a reaction in the microfluidic chamber, an illumination source to provide the excitation, and a detection system to detect fluorescence generated by the excitation of the fluorophores in the microfluidic chamber.

公开(公告)号：US20230273233A1

公开(公告)日：2023-08-31

申请号：US18015044

申请日：2020-07-20

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Roberto A. Pugliese ,  Jeffrey A. Nielsen ,  Michael J. Day ,  Diane R. Hammerstad

IPC: G01N35/10 ,  B01L3/02

CPC classification number: G01N35/1016 ,  B01L3/0268 ,  B01L2200/061

Abstract: In one example in accordance with the present disclosure, a particle dispensing system is described. The particle dispensing system includes a port to receive a number of fluid cartridges. Each fluid cartridge is to hold an amount of fluid to be ejected. The particle dispensing system also includes an optical verification system to determine, following ejection, a count of a number of particles ejected during an ejection event. The particle dispensing system also includes a controller to selectively activate a number of fluid ejectors to eject the amount of fluid.

公开(公告)号：US11725175B2

公开(公告)日：2023-08-15

申请号：US16605193

申请日：2017-09-23

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Diane R. Hammerstad ,  Michael W. Cumbie

IPC: C12M1/42 ,  C12M1/26 ,  C12M1/36 ,  C12N13/00 ,  C12N15/87 ,  C12M3/00 ,  A61N1/10 ,  C12M1/00

CPC classification number: C12M35/02 ,  C12M1/42 ,  C12M3/006 ,  C12M33/04 ,  C12M41/48 ,  C12N13/00 ,  C12N15/87 ,  A61N1/10 ,  C12M45/07

Abstract: An electroporation system may include a well plate, a dispenser and a dispenser-well positioning system. The well plate may include wells, each of the wells including an interior, a first electrode adjacent the interior and a second electrode adjacent the interior and spaced from the first electrode. The first electrode and the second electrode are to apply an electrostatic field across the well. The dispenser is to dispense a cell having a diameter into each of the wells. The dispenser-well positioning system is to align each well and the dispenser such that the dispenser dispenses the cell into each well at a location spaced from the first electrode and the second electrode by a distance of at least 5 times the diameter of the cell.

公开(公告)号：US20230234052A1

公开(公告)日：2023-07-27

申请号：US18012219

申请日：2020-07-07

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Fausto D'Apuzzo ,  Viktor Shkolnikov ,  Alexander N. Govyadinov

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/168 ,  B01L2300/0636

Abstract: A microfluidic sensing assembly may include a first structure supporting a sensor array, a second structure joined to the first structure and forming a microfluidic passage and a flat lens to focus light, following reflection of the light back and forth across the microfluidic passage, from the microfluidic passage onto the sensor array.

公开(公告)号：US20230050391A1

公开(公告)日：2023-02-16

申请号：US17974743

申请日：2022-10-27

Applicant: Hewlett-Packard Development Company, L.P.

Inventor： Viktor Shkolnikov ,  Chien-Hua CHEN

IPC: B01L7/00 ,  B01L3/00 ,  G01N33/543

Abstract: An example system includes an array of retaining features in a microfluidic cavity, an array of thermally controlled releasing features, and a controller coupled to each releasing feature in the array of releasing feature. Each retaining feature in the array of retaining features is to position capsules at a predetermined location, the capsules having a thermally degradable shell enclosing a biological reagent therein. Each releasing feature in the array of releasing features corresponds to a retaining feature and is to selectively cause degradation of the shell of a capsule. Each releasing feature is to generate thermal energy to facilitate degradation of the shell. The controller is to selectively activate at least one releasing feature in the array of thermally controlled releasing features to release the biological reagent in the capsules positioned at the retaining feature corresponding to the activated releasing feature.

公开(公告)号：US11577252B2

公开(公告)日：2023-02-14

申请号：US16605780

申请日：2018-04-27

Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

Inventor： Viktor Shkolnikov ,  Chien-Hua Chen

IPC: B01L3/00 ,  B01L7/00 ,  G01N33/543

Abstract: An example system includes an array of retaining features in a microfluidic cavity, an array of thermally controlled releasing features, and a controller coupled to each releasing feature in the array of releasing feature. Each retaining feature in the array of retaining features is to position capsules at a predetermined location, the capsules having a thermally degradable shell enclosing a biological reagent therein. Each releasing feature in the array of releasing features corresponds to a retaining feature and is to selectively cause degradation of the shell of a capsule. Each releasing feature is to generate thermal energy to facilitate degradation of the shell. The controller is to selectively activate at least one releasing feature in the array of thermally controlled releasing features to release the biological reagent in the capsules positioned at the retaining feature corresponding to the activated releasing feature.