公开(公告)号：US5358639A

公开(公告)日：1994-10-25

申请号：US38396

申请日：1993-03-29

申请人： Kenji Yasuda ,  Yoshinori Takata ,  Harumi Tsuruta ,  Sadabumi Ohnuma ,  Junkichi Miura

发明人： Kenji Yasuda ,  Yoshinori Takata ,  Harumi Tsuruta ,  Sadabumi Ohnuma ,  Junkichi Miura

IPC分类号： G01N30/26 ,  G01N30/02 ,  G01N30/06 ,  G01N30/24 ,  G01N30/34 ,  G01N30/50 ,  G01N30/52 ,  G01N30/88 ,  G01N33/72 ,  B01D15/08

CPC分类号： G01N30/52 ,  G01N2030/525 ,  G01N2030/8813 ,  G01N2030/8822 ,  G01N30/02 ,  G01N30/06 ,  G01N30/24 ,  G01N30/34 ,  G01N33/72

摘要： In the analysis of hemoglobins in blood samples by chromatography, an increase in the pressure in a flow passage line is suppressed, a separation column is made durable against prolonged use by supplying phosphate-based buffer solutions as eluting solutions to a separation column to a separation column and a solution containing not more than 100 mM of S-(carboxyalkyl)-L-cysteine and phosphate-based buffer agent thereto in the course of a series of analyzing steps.

摘要翻译： 在通过色谱法对血液样品中的血红蛋白进行分析时，抑制流路线中的压力增加，通过将磷酸盐类缓冲溶液作为分离柱的洗脱溶液供给至分离液，使分离塔长期使用耐久性 柱和在一系列分析步骤中含有不超过100mM的S-（羧基烷基）-L-半胱氨酸和磷酸盐缓冲剂的溶液。

公开(公告)号：US5252533A

公开(公告)日：1993-10-12

申请号：US856796

申请日：1992-03-24

申请人： Kenji Yasuda ,  Toshihiro Minato ,  Masaru Kato ,  Toshikazu Nagura ,  Hiroshi Arakawa

发明人： Kenji Yasuda ,  Toshihiro Minato ,  Masaru Kato ,  Toshikazu Nagura ,  Hiroshi Arakawa

IPC分类号： B41M5/41 ,  B41M5/035 ,  B41M5/38

CPC分类号： B41M5/41 ,  Y10S428/913 ,  Y10S428/914 ,  Y10T428/24942 ,  Y10T428/2495 ,  Y10T428/24967 ,  Y10T428/24975 ,  Y10T428/24992 ,  Y10T428/31511 ,  Y10T428/31786 ,  Y10T428/31797 ,  Y10T428/31855

摘要： A thermal transfer dye image-receiving sheet capable of receiving clear, uniform colored images without a formation of curls and wrinkles therein, comprising (A) a substrate sheet composed of (a) core sheet having a Young's modulus E.sub.3, (b) a front coated thermoplastic film layer having a thickness T.sub.1 and a Young's modulus E.sub.1, and (c) a back coated thermoplastic film layer having a thickness T.sub.2 and a Young's modulus E.sub.2, the T.sub.1, T.sub.2, E.sub.1, E.sub.2 and E.sub.3 satisfying the following relationships (1) and (2):T.sub.2 .gtoreq.T.sub.1 (1)andE.sub.3 .gtoreq.2E.sub.1 .gtoreq.2E.sub.2 (2)wherein Y.sub.1, Y.sub.2 and Y.sub.3 are determined in accordance with ASTM D882-64T.

摘要翻译： 一种热转印染料影像接收片材，其能够接收清晰均匀的彩色图像而不形成卷曲和皱纹，其包括（A）由（a）具有杨氏模量E3的芯片组成的基片，（b） 具有厚度T1和杨氏模量E1的涂布热塑性薄膜层，（c）具有厚度T2和杨氏模量E2，T1，T2，E1，E2和E3的背涂热塑性薄膜层，满足以下关系式（1 ）和（2）：T2> / = T1（1）和E3> / = 2E1> / = 2E2（2）其中Y1，Y2和Y3根据ASTM D882-64T测定。

公开(公告)号：US5057438A

公开(公告)日：1991-10-15

申请号：US31665

申请日：1987-03-30

申请人： Kazumichi Imai ,  Daizo Tokinaga ,  Teruaki Kobayashi ,  Kenji Yasuda ,  Keiichi Nagai ,  Satoshi Takahashi

发明人： Kazumichi Imai ,  Daizo Tokinaga ,  Teruaki Kobayashi ,  Kenji Yasuda ,  Keiichi Nagai ,  Satoshi Takahashi

IPC分类号： G01N33/561

CPC分类号： G01N33/561 ,  Y10S436/809 ,  Y10S436/824

摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.

摘要翻译： 通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定 反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体 并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。

公开(公告)号：US4971950A

公开(公告)日：1990-11-20

申请号：US367075

申请日：1989-06-16

申请人： Masaru Kato ,  Toshihiro Minato ,  Toshikazu Nagura ,  Susumu Hasegawa ,  Kenji Yasuda

发明人： Masaru Kato ,  Toshihiro Minato ,  Toshikazu Nagura ,  Susumu Hasegawa ,  Kenji Yasuda

IPC分类号： B41M5/26 ,  B41M5/382 ,  B41M5/41 ,  B41M5/42 ,  H02G5/06

CPC分类号： B41M5/42 ,  B41M5/38214 ,  B41M5/41 ,  Y10S428/91 ,  Y10S428/913 ,  Y10S428/914 ,  Y10T428/31913 ,  Y10T428/31938

摘要： A support sheet useful for a thermal transfer image receiving sheet having a high resistance to thermal curling and capable of recording clear and uniform images or pictures thereon, comprises a sheet substrate with a thickness of 20 to 300 .mu.m, and multilayer front and back surface coating plastic films each comprising a pigment and a polyolefin resin, each having at least one bi-axially oriented base layer, and bonded to the sheet substrate, and satisfies the following relationships:S.sub.1 /S.sub.2 .ltoreq.1/2S.sub.3

公开(公告)号：US4824778A

公开(公告)日：1989-04-25

申请号：US867554

申请日：1986-05-28

申请人： Keiichi Nagai ,  Daizo Tokinaga ,  Kazumichi Imai ,  Kenji Yasuda ,  Satoshi Takahashi ,  Teruaki Kobayashi

发明人： Keiichi Nagai ,  Daizo Tokinaga ,  Kazumichi Imai ,  Kenji Yasuda ,  Satoshi Takahashi ,  Teruaki Kobayashi

IPC分类号： G01N33/543 ,  G01N33/561 ,  G01N33/53

CPC分类号： G01N33/561 ,  Y10S435/968

摘要： An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluorescent substance in the electrolyte solution.

摘要翻译： 免疫测定法，其包括将抗体固定在用于电泳的基质中; 通过使抗原通过电泳移动的方法，将抗原固定在测量样品中，使其与上述固定化抗体进行抗原抗体反应; 通过电泳将标记抗体移动到上述固定化抗原上，使其与固定化抗原反应，或通过电泳将标记的抗原移动到上述固定化抗体的未反应部分，使其与未反应部分反应; 测定样品中的抗原浓度，其特征在于，将标记抗体或标记抗原的标记作为能够将底物覆盖成荧光物质的酶，通过电泳将所述酶转化为荧光物质， 使底物与标记酶反应，将其转化为荧光物质，并测量电解质溶液中荧光物质的浓度。

公开(公告)号：US4541717A

公开(公告)日：1985-09-17

申请号：US559366

申请日：1983-12-08

申请人： Shigeru Itamoto ,  Kenji Yasuda ,  Senji Mikami

发明人： Shigeru Itamoto ,  Kenji Yasuda ,  Senji Mikami

IPC分类号： B25B11/00 ,  G01B5/00 ,  G01B9/02

CPC分类号： B25B11/005 ,  G01B5/0007 ,  G01B9/02

摘要： A device for holding a measurement object e.g. in interferometers comprises a base plate and an attraction plate mounted on the base plate so as to attract the object placed on an upper surface of the attraction plate to the base plate together with the attraction plate by a vacuum applied via attraction grooves formed on the base plate and attraction passages perforated through the attraction plate. The upper surface of the base plate is formed as a reflection plane, and the attraction plate is made of a transparent material, so that Newton's rings generated by light reflected from the upper surface of the base plate and light reflected from the lower surface of the attraction plate can be observed from above the attraction plate.

摘要翻译： 一种用于保持测量对象的设备，例如 在干涉仪中包括基板和安装在基板上的吸引板，以便通过在底座上形成的吸引槽将吸引板的上表面的物体与吸引板一起吸引到基板上 板和吸引通道穿过吸引板。 基板的上表面形成为反射面，吸引板由透明材料制成，从而从基板的上表面反射的光产生的牛顿环和从底板的下表面反射的光 吸引板上方可观察到吸引板。

公开(公告)号：US08642306B2

公开(公告)日：2014-02-04

申请号：US12663373

申请日：2008-06-11

申请人： Shigeyuki Nakada ,  Noriaki Kimura ,  Akihide Ito ,  Kenji Yasuda

发明人： Shigeyuki Nakada ,  Noriaki Kimura ,  Akihide Ito ,  Kenji Yasuda

IPC分类号： C12N13/00

CPC分类号： G01N15/147 ,  C12M47/04 ,  G01N2015/149

摘要： A liquid sample flow containing living cells is irradiated with measurement laser light and the photo data of at least either scattering light or fluorescence that is generated by each of the living cells in the liquid sample flow due to the irradiation with the measurement laser light is acquired. Based on the photo data thus acquired, it is determined whether each of the cells assignable to the respective photo data is an unnecessary living cell or a target living cell. Based on the determination results, a pulse voltage is then applied exclusively to the living cells having been determined as unnecessary living cells so that the unnecessary living cells are damaged and killed.

摘要翻译： 用测量激光照射含有活细胞的液体样品流，并且获取由于测量激光的照射而在液体样品流中的每个活细胞产生的至少一种散射光或荧光的照片数据 。 基于这样获得的照片数据，确定分配给各个照片数据的每个细胞是否是不必要的活细胞或目标活细胞。 基于测定结果，脉冲电压被专门用于被确定为不必要的活细胞的活细胞，使得不必要的活细胞被损坏和死亡。

公开(公告)号：US20130314526A1

公开(公告)日：2013-11-28

申请号：US13882257

申请日：2011-09-16

申请人： Kenji Yasuda ,  Hideyuki Terazono ,  Hyonchol Kim ,  Masahito Hayashi ,  Akihiro Hattori ,  Kazuto Nishio ,  Tokuzo Arao ,  Yohei Miyagi ,  Takashi Ohtsu

发明人： Kenji Yasuda ,  Hideyuki Terazono ,  Hyonchol Kim ,  Masahito Hayashi ,  Akihiro Hattori ,  Kazuto Nishio ,  Tokuzo Arao ,  Yohei Miyagi ,  Takashi Ohtsu

IPC分类号： G06T7/00

CPC分类号： G06T7/0016 ,  G01N15/1475 ,  G01N15/1484 ,  G01N27/447 ,  G01N33/487 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1497

摘要： Provided is a cell concentration/purification device having a function of successively locating cells in a specific area of a microchannel, and a function of sequentially capturing cell images by use of light from a plurality of monochromatic light sources on an image basis, and performing comparative analysis of the cell images to recognize individual cells based on information on the shape of the cells and an absorption spectral distribution of the cells or inside of the cells, thereby selectively separating/purifying the cells.

摘要翻译： 本发明提供一种细胞浓度/净化装置，其具有将细胞连续定位在微通道的特定区域中的功能，以及通过使用来自多个单色光源的光以图像为基础顺序捕获细胞图像的功能，并进行比较 基于关于细胞形状的信息和细胞的吸收光谱分布或细胞内部的信息来分析细胞图像以识别个体细胞，从而选择性地分离/纯化细胞。

公开(公告)号：US20130230881A1

公开(公告)日：2013-09-05

申请号：US13876992

申请日：2011-09-30

申请人： Kenji Yasuda ,  Tomoyuki Kaneko ,  Fumimasa Nomura

发明人： Kenji Yasuda ,  Tomoyuki Kaneko ,  Fumimasa Nomura

IPC分类号： C12Q1/02

CPC分类号： C12Q1/025 ,  C12M35/02 ,  C12M41/46 ,  C12M41/48 ,  G01N33/48728 ,  G01N33/5061 ,  G01N33/5088 ,  G01N2800/32

摘要： In the present invention, a cardiomyocyte cluster is disposed on a transparent substrate, and the quality of the cardiomyocytes is evaluated from the response of the cells to a forced pulsation stimulus applied to the cardiomyocytes. The cardiomyocyte cluster is disposed on the transparent substrate, and is exposed to the flow of a liquid containing an agent in a manner so that the agent acts on the cells, which configure a network. The extent of cardiac toxicity resulting from the agent is evaluated from measuring the fluctuations obtained from a comparison of adjacent cardiomyocytes of the network.

摘要翻译： 在本发明中，将心肌细胞簇设置在透明基质上，根据细胞对施加于心肌细胞的强制脉动刺激的反应来评价心肌细胞的质量。 将心肌细胞簇设置在透明基板上，并且以使得试剂作用于构成网络的细胞的方式暴露于含有试剂的液体的流动。 通过测量从网络的相邻心肌细胞的比较获得的波动来评估由该试剂产生的心脏毒性的程度。

公开(公告)号：US20130029407A1

公开(公告)日：2013-01-31

申请号：US13261410

申请日：2011-02-24

申请人： Hideyuki Terazono ,  Kenji Yasuda ,  Masahito Hayashi ,  Hiroyuki Takei ,  Akihiro Hattori

发明人： Hideyuki Terazono ,  Kenji Yasuda ,  Masahito Hayashi ,  Hiroyuki Takei ,  Akihiro Hattori

IPC分类号： C12M1/42

CPC分类号： G01N1/31 ,  C12N1/066 ,  G01N1/34 ,  G01N1/4077 ,  G01N15/1459 ,  G01N15/1463 ,  G01N21/6408 ,  G01N21/6428 ,  G01N2001/4094 ,  G01N2015/149 ,  G01N2021/6482 ,  Y10T436/11

摘要： Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof.

摘要翻译： 提供一种细胞浓缩和净化装置，其具有：连续浓缩细胞的功能; 随后随后将细胞连续地放置在通道的特定区域中的功能; 基于图像同时识别每个单个细胞的形状和荧光发射的功能; 以及基于与其形状和荧光发射有关的数据识别细胞，然后分离和纯化细胞的功能。