公开(公告)号：US11919971B2

公开(公告)日：2024-03-05

申请号：US16302023

申请日：2017-05-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Daniel Christopher Leslie ,  Thomas Doyle ,  Anna Waterhouse ,  Melissa Rodas ,  Alexander L. Watters ,  Michael Super ,  Donald E. Ingber

IPC: C07K17/08 ,  C08L25/06 ,  C08L83/04 ,  G01N33/543 ,  G01N33/545 ,  C08K5/05

CPC classification number: C07K17/08 ,  C08L25/06 ,  C08L83/04 ,  G01N33/54393 ,  G01N33/545 ,  C08K5/05 ,  C08L2203/02

Abstract: This disclosure provides, e.g., methods for coupling Formation of Surface Carboxylates on PES an entity to a solid substrate. The method can comprise treating the with Exposure Time substrate with a plasma, e.g., a CO2 plasma, to increase its reactivity. The entity can be, e.g., a biological polymer that binds a microbe. Substrates produced by these methods can be used in a variety of applications, including hemodialysis and diagnostic assays.

公开(公告)号：US20240002808A1

公开(公告)日：2024-01-04

申请号：US18226184

申请日：2023-07-25

Applicant: President and Fellows of Harvard College

Inventor： Richard Novak ,  Sasan Jalili-Firoozinezhad ,  Francesca S. Gazzaniga ,  Elizabeth Calamari ,  Diogo M. Camacho ,  Bret Andrew Nestor ,  Cicely Fadel ,  Michael J. Cronce ,  Dennis Kasper ,  Donald E. Ingber ,  Amir Bein

IPC: C12N5/071 ,  C12M3/00 ,  C12M3/06 ,  C12N1/20

CPC classification number: C12N5/0679 ,  C12N1/20 ,  C12M23/16 ,  C12M21/08

Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.

公开(公告)号：US11807677B2

公开(公告)日：2023-11-07

申请号：US17552662

申请日：2021-12-16

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander Watters ,  Brendon Dusel ,  Michael Super ,  Mark Cartwright ,  Donald E. Ingber

IPC: C07K14/78 ,  G01N33/569 ,  C07K14/00 ,  C07K14/47 ,  C07K19/00 ,  A61K38/00

CPC classification number: C07K14/78 ,  C07K14/00 ,  C07K14/4726 ,  C07K19/00 ,  G01N33/569 ,  A61K38/00 ,  C07K2319/30 ,  G01N2333/78 ,  G01N2400/00

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e g, enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.

公开(公告)号：US20230348831A1

公开(公告)日：2023-11-02

申请号：US18346165

申请日：2023-06-30

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Anthony M. Bahinski ,  Robert Cunningham ,  Josue A. Goss ,  Geraldine A. Hamilton ,  Christopher David Hinojosa ,  Daniel Levner ,  Kevin Kit Parker

IPC: C12M3/06 ,  A01N1/02 ,  C12M1/34 ,  G01N33/50 ,  B01L3/00 ,  B01L9/00 ,  C12M3/00 ,  C12M1/00 ,  C12M1/42

CPC classification number: C12M23/16 ,  A01N1/02 ,  C12M41/00 ,  G01N33/5082 ,  B01L3/502715 ,  B01L9/52 ,  C12M21/08 ,  C12M23/42 ,  C12M29/10 ,  C12M35/04 ,  C12M35/08 ,  G01N33/5088 ,  A01N1/0247 ,  G01N33/5005 ,  A01N1/0273 ,  A01N1/021 ,  B01L3/5027 ,  C12M41/46 ,  C12M41/12 ,  B01L2300/0877 ,  B01L2300/0867 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/123 ,  B01L2300/18 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/0644

Abstract: The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.

公开(公告)号：US11654399B2

公开(公告)日：2023-05-23

申请号：US15558191

申请日：2016-03-16

Applicant: President and Fellows of Harvard College

Inventor： David James Coon ,  Tiama Hamkins-Indik ,  Donald E. Ingber ,  Miles Ingram ,  Daniel Levner ,  Richard Novak ,  Jefferson Puerta ,  Daniel E. Shea ,  Josiah Sliz ,  Norman Wen

IPC: B01D67/00 ,  B29C41/38 ,  B29C33/42 ,  B29C33/68 ,  B29C39/00 ,  B29C39/26 ,  B29C41/12 ,  B01L3/00 ,  B29C41/36 ,  B01D71/70 ,  C12M1/12 ,  B29L31/00 ,  B29C43/02

CPC classification number: B01D67/0034 ,  B01D71/70 ,  B01L3/502707 ,  B29C33/424 ,  B29C33/68 ,  B29C39/006 ,  B29C39/26 ,  B29C41/12 ,  B29C41/36 ,  B29C41/38 ,  C12M25/02 ,  B01L2300/123 ,  B01L2400/086 ,  B29C2043/025 ,  B29L2031/755 ,  B29L2031/756

Abstract: A method for micro-molding a polymeric membrane and including pouring a predetermined volume of curable polymer unto a micro-fabricated mold having a post array with pillars, and overlaying the polymer with a support substrate. A spacer, such as a rubber spacer, is placed in contact with the support substrate and a force is applied to an exposed side of the spacer to compress the support substrate and the polymer together. While applying the force, the polymer is cured on the mold for a predetermined time period and at a predetermined temperature to form a polymeric membrane having a pore array with a plurality of pores corresponding to the plurality of pillars of the post array. The polymeric membrane is removed from the support substrate.

公开(公告)号：US20220372423A1

公开(公告)日：2022-11-24

申请号：US17871054

申请日：2022-07-22

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Daniel LEVNER ,  Guy THOMPSON, III ,  Jose FERNANDEZ-ALCON ,  Christopher David HINOJOSA

IPC: C12M1/00 ,  C12M3/06 ,  B01L3/00 ,  C12M3/00 ,  C12M1/12 ,  C12M1/36

Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.

公开(公告)号：US11434458B2

公开(公告)日：2022-09-06

申请号：US15403942

申请日：2017-01-11

Applicant: President and Fellows of Harvard College

Inventor： Christopher David Hinojosa ,  Josiah Sliz ,  Daniel Levner ,  Guy Thompson ,  Hubert Geisler ,  Jose Fernandez-Alcon ,  Donald E. Ingber

IPC: C12M3/06 ,  B01L3/00 ,  B82Y15/00 ,  C12M1/34 ,  G01N33/50 ,  C12M3/00 ,  C12M1/26

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

公开(公告)号：US20220195017A1

公开(公告)日：2022-06-23

申请号：US17565783

申请日：2021-12-30

Applicant: President and Fellows of Harvard College

Inventor： Donald E. Ingber ,  Michael Super ,  Alexander L. Watters

IPC: C07K16/12 ,  G01N33/487 ,  A61P31/04 ,  G01N33/543 ,  G01N33/569 ,  C07K16/14 ,  C07K14/705

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. The microbe-targeting or microbe-binding molecules can comprise a microbe surface-binding domain linked to a portion of an Fc region. Further, the microbe-targeting molecules can be conjugated to substrate (e.g., a magnetic particle) to form a microbe-targeting substrate. Such microbe-targeting molecules and/or substrates and the kits comprising the same can be used in various applications, such as diagnosis and/or treatment of an infection caused by microbes. Moreover, the microbe-targeting molecules and/or substrates can be easily regenerated after use.

公开(公告)号：US20220089989A1

公开(公告)日：2022-03-24

申请号：US17540619

申请日：2021-12-02

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Donald E. Ingber ,  Andries Van der Meer ,  Anna Herland

IPC: C12M3/06 ,  C12M1/12 ,  C12M1/00 ,  C12M1/42 ,  C12M1/34 ,  C12N5/079 ,  G01N33/50

Abstract: Systems and methods for producing and using a body having a first structure defining a first chamber, a second structure defining a second chamber, a membrane located at an interface region between the first chamber and the second chamber to separate the first chamber from the second chamber. The first chamber comprises a first permeable matrix disposed therein and the first permeable matrix comprises at least one or a plurality of lumens each extending therethrough, which is optionally lined with at least one layer of cells. The second chamber can comprise cells cultured therein. The systems and methods described herein can be used for various applications, including, e.g., growth and/or differentiation of primary cells, and/or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents). The systems and methods can also permit co-cultures of two or more different cell types.

公开(公告)号：US11034926B2

公开(公告)日：2021-06-15

申请号：US15403949

申请日：2017-01-11

Applicant: President and Fellows of Harvard College

Inventor： Christopher David Hinojosa ,  Josiah Sliz ,  Daniel Levner ,  Guy Thompson ,  Hubert Geisler ,  Jose Fernandez-Alcon ,  Donald E. Ingber

IPC: C12M3/06 ,  B01L3/00 ,  B82Y15/00 ,  C12M1/34 ,  G01N33/50 ,  C12M3/00 ,  C12M1/26

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.