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104 条记录 (耗时 0.037 秒)

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    92.
    发明申请
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
    DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物

    公开(公告)号：US20070281340A1

    公开(公告)日：2007-12-06

    申请号：US11607900

    申请日：2006-12-04

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12P19/34

    CPC分类号： C12Q1/6869 ,  C12Q2535/125

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is G, while a base adjacent with one base spaced apart is T.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be T or G; the third base from the 3′ end to be G or C; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be completely complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当与目标SNP碱基的3'侧紧邻的碱基为G时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开。根据本发明，3'端基被设计为对应于SNP的基座; 从3'端的第二个基地是T或G; 从3'的第三个基地是G或C; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到碱基的碱基三碱基的序列完全互补。

    Discrimination method of target base in DNA, and allele specific primer used in the method of the same
    93.
    发明授权
    Discrimination method of target base in DNA, and allele specific primer used in the method of the same 有权
    DNA中靶基因的鉴别方法及其方法中使用的等位基因特异性引物

    公开(公告)号：US07297496B2

    公开(公告)日：2007-11-20

    申请号：US11333352

    申请日：2006-01-18

    申请人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    发明人： Hidenobu Yaku ,  Hiroaki Oka ,  Tetsuo Yukimasa

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6813

    摘要： An object of the present invention is to provide an allele specific primer which is accompanied by less possibility of the false positive and enables definite discrimination when a base immediately adjacent to on the 3′ side of a target SNP base is A, while a base adjacent with one base spaced apart is T.According to the present invention, the 3′ end base is designed to be the base corresponding to SNP; the second base from the 3′ end to be C; the third base from the 3′ end to be any one of T, C or G; and the base sequence of from the fourth from the 3′ end to the 5′ end base to be complementary to the sequence of from a base three bases away from the target SNP base on the 3′ side to a desired base.

    摘要翻译： 本发明的目的是提供一种等位基因特异性引物，当靶SNP碱基的3'侧邻近的碱基为A时，伴随着假阳性的可能性较小，并且能够进行确定的鉴别，而邻近的碱基 其中一个基座间隔开。根据本发明，3'端基被设计为对应于SNP的基座; 从3'的第二个底部到C; 从3'端的第三个基地是T，C或G的任何一个; 并且从3'末端到5'末端碱基的第四个碱基序列与从3'侧的目标SNP碱基到3碱基的碱基的序列互补到所需的碱基。

    Apparatus and method for measuring activity signals of biological samples
    94.
    发明申请
    Apparatus and method for measuring activity signals of biological samples 有权

    公开(公告)号：US20070134651A1

    公开(公告)日：2007-06-14

    申请号：US11652605

    申请日：2007-01-12

    申请人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

    发明人： Hiroaki Oka ,  Nobuhiko Ozaki ,  Hirokazu Sugihara

    IPC分类号： C12Q1/00 ,  G06F19/00

    CPC分类号： G01N33/48728 ,  G01N33/54373 ,  G01N33/5438

    摘要： The present invention provides an apparatus for measuring activity signals of a biological sample comprising: a measurement chamber (A) storing a target liquid containing a biological sample; a porous insulating substrate (5) provided with a measurement electrode (1) on at least one side; and a conveying device (8) which conveys the target liquid stored in the measurement chamber (A) and passes the target liquid through the porous insulating substrate (5) from the measurement electrode (1) side, wherein the conveying device (8) is operated to trap the biological sample contained in the target liquid onto the measurement electrode (1), so that the activity signals of the biological sample are measured through the measurement electrode (1). According to the apparatus, activity signals emitted from the biological sample can be detected easily, rapidly and accurately.

    Method of measuring pyrophosphate and method of detecting primer extension reaction, and device for performing the same
    98.
    发明申请
    Method of measuring pyrophosphate and method of detecting primer extension reaction, and device for performing the same 审中-公开
    测定焦磷酸盐的方法和检测引物延伸反应的方法及其执行方法

    公开(公告)号：US20060211005A1

    公开(公告)日：2006-09-21

    申请号：US11180881

    申请日：2005-07-14

    申请人： Hidenobu Yaku ,  Masayoshi Maeshima ,  Yoichi Nakanishi ,  Megumi Hirono ,  Tetsuo Yukimasa ,  Hiroaki Oka

    发明人： Hidenobu Yaku ,  Masayoshi Maeshima ,  Yoichi Nakanishi ,  Megumi Hirono ,  Tetsuo Yukimasa ,  Hiroaki Oka

    IPC分类号： C12Q1/68 ,  C12Q1/34

    CPC分类号： B82Y15/00 ,  B82Y30/00 ,  C12Q1/42 ,  C12Q1/6846 ,  G01N33/56911 ,  C12Q2565/301

    摘要： An object of the present invention is to provide a method of quantitative determination of pyrophosphate (PPi) and a method of detecting a primer extension reaction that require fewer kinds of enzymes and that do not necessitate strict temperature regulation, and a kit and a device for performing these methods. In the method of measuring PPi of the present invention, a PPi sample having an unknown concentration is allowed to act on mycobacterial H+-pyrophosphatase (H+-PPase) intrinsically included in membrane, thereby measuring the PPi concentration in the sample by analyzing thus resulting H+transport. Furthermore, in the method of detecting an extension reaction of a primer of the present invention, an unknown nucleic acid sample to which examination of occurrence of the primer extension reaction is intended is allowed to act on mycobacterial H+-PPase intrinsically included in a membrane, thereby determining occurrence of the primer extension reaction on the unknown nucleic acid sample by analyzing thus resulting H+transport.

    摘要翻译： 本发明的目的是提供一种定量测定焦磷酸盐（PPi）的方法和检测需要更少种类的酶并且不需要严格的温度调节的引物延伸反应的方法，以及用于 执行这些方法。 在本发明的PPi测定方法中，使具有未知浓度的PPi试样作用于本质上包含的分枝杆菌H + + - 磷酸酶（H + + - 磷酸酶） 从而通过分析由此得到的H +运输来测量样品中的PPi浓度。 此外，在检测本发明的引物的延伸反应的方法中，允许对引物延伸反应的出现进行检查的未知核酸样品作用于分枝杆菌H + - 本质上包含在膜中，从而通过分析由此产生的H +运输来确定未知核酸样品上的引物延伸反应的发生。

    Extracellular recording electrode
    99.
    发明授权
    Extracellular recording electrode 有权
    细胞外记录电极

    公开(公告)号：US07041492B2

    公开(公告)日：2006-05-09

    申请号：US10048662

    申请日：2001-06-20

    申请人： Hiroaki Oka ,  Tetsuo Yukimasa ,  Ryuta Ogawa ,  Hirokazu Sugihara ,  Katsuyuki Tsuji ,  Yukifumi Yoshimoto

    发明人： Hiroaki Oka ,  Tetsuo Yukimasa ,  Ryuta Ogawa ,  Hirokazu Sugihara ,  Katsuyuki Tsuji ,  Yukifumi Yoshimoto

    IPC分类号： C12M1/42

    CPC分类号： A61N1/0492 ,  A61N1/0476 ,  A61N1/0551 ,  G01N33/4836

    摘要： A multiple electrode includes a plurality of micro-electrodes provided on a substrate, and a wiring portion for providing an electrical signal to the micro-electrodes or extracting an electrical signal from the micro-electrodes. Each micro-electrode has porous conductive material on its surface, and the impedance of the micro-electrode is 50 kΩ or less. Preferably, the porous conductive material is gold, and formed by the passage of current at a current density of 1.0 to 5.0 A/dm2 for 10 to 360 sec. The multiple electrode may include micro-electrodes provided on a substrate in the form of a matrix, a lead line connected to the micro-electrodes, and an electrical junction connected to an end of the lead line.

    摘要翻译： 多个电极包括设置在基板上的多个微电极和用于向微电极提供电信号或从微电极提取电信号的布线部分。 每个微电极在其表面上具有多孔导电材料，微电极的阻抗为50kΩ以下。 优选地，多孔导电材料是金，并且通过电流以1.0至5.0A / dm 2的电流通过10至360秒形成。 多个电极可以包括以矩阵形式设置在基板上的微电极，连接到微电极的引线和连接到引线的端部的电连接。