公开(公告)号：US06369294B1

公开(公告)日：2002-04-09

申请号：US09819964

申请日：2001-03-28

申请人： Jorge A. Piedrahita ,  Fuller W. Bazer

发明人： Jorge A. Piedrahita ,  Fuller W. Bazer

IPC分类号： C12N1585

CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/075 ,  C12N5/0611 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/235 ,  C12N2502/13 ,  C12N2510/00 ,  C12N2517/04 ,  C12N2517/10

摘要： Disclosed are methods for the isolation of primordial germ cells, culturing these cells to produce primordial germ cell-derived cell lines, methods for transforming both the primordial germ cells and the cultured cell lines, and using these transformed cells and cell lines to generate transgenic animals. The efficiency at which transgenic animals are generated by the present invention is greatly increased, thereby allowing the use of homologous recombination in producing transgenic non-rodent animal species.

摘要翻译： 公开了分离原始生殖细胞，培养这些细胞以产生原始生殖细胞来源的细胞系的方法，用于转化原始生殖细胞和培养的细胞系的方法，以及使用这些转化的细胞和细胞系产生转基因动物 。 通过本发明产生转基因动物的效率大大增加，从而允许在产生转基因非啮齿类动物物种中使用同源重组。

公开(公告)号：US06245566B1

公开(公告)日：2001-06-12

申请号：US09052772

申请日：1998-03-31

申请人： John D. Gearhart ,  Michael Joseph Shamblott

发明人： John D. Gearhart ,  Michael Joseph Shamblott

IPC分类号： C12N502

CPC分类号： C12N5/0611 ,  C12N5/0603 ,  C12N2501/01 ,  C12N2501/115 ,  C12N2501/235 ,  C12N2502/1323 ,  G01N33/56966 ,  G01N33/689

摘要： Primordial germ cells isolated from human embryonic tissue, such as from the gonadal ridges of human embryo, are disclosed. The primordial germ cells are cultured resulting in cells that resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.

摘要翻译： 公开了从人胚胎组织分离的原始生殖细胞，例如来自人胚胎的性腺脊髓。 培养原始生殖细胞，产生类似胚胎干细胞或胚胎生殖细胞的形态和多能性的细胞。 细胞在培养物中保持数月，可以使用转基因技术进行遗传操作以插入异源遗传物质。

公开(公告)号：US20240318134A1

公开(公告)日：2024-09-26

申请号：US18579409

申请日：2022-07-22

申请人： INSTITUT PASTEUR ,  ECOLE POLYTECHNIQUE ,  INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE

发明人： Charles BAROUD ,  Jack Christophe COSSEC ,  Anne DEJEAN ,  Sebastien SART ,  Tatiana TRABOULSI

IPC分类号： C12N5/0735 ,  A61K35/54 ,  C12M1/12 ,  C12M3/06 ,  C12N5/00 ,  G01N33/50

CPC分类号： C12N5/0611 ,  A61K35/54 ,  C12M23/16 ,  C12M25/14 ,  C12N5/0018 ,  G01N33/5026 ,  G01N33/5082 ,  C12N2501/235 ,  C12N2501/72 ,  C12N2506/45 ,  C12N2513/00 ,  G01N2500/10

摘要： The invention relates to in vitro generation of organized 3D cell structures recapitulating various degrees of early organogenesis, including head-trunk embryo-like structures, using epigenetic remodeling factors. The invention relates in particular to methods of obtaining such organized 3D cell structures from mammalian cells, and to devices, in particular microfluidic platform, to perform such methods. The invention also concerns the use of the thus obtained 3D cell structures in applications of molecule screening, developmental testing, production of physiologically active substances and models for therapeutic investigation or use.

公开(公告)号：US20240052304A1

公开(公告)日：2024-02-15

申请号：US18269131

申请日：2021-12-30

申请人： THE STATE OF ISRAEL MINISTRY OF AGRICULTURE & RURAL DEVELOPMENT, AGRICULTURAL RESEARCH ORGANIZATION

发明人： Yuval CINNAMON ,  Enbal BEN-TAL COHEN

IPC分类号： C12N5/0735 ,  A01K67/027

CPC分类号： C12N5/0611 ,  A01K67/0275 ,  C12N2510/00 ,  A01K2217/075 ,  A01K2227/30

摘要： The present disclosure relates to deoxyribonucleic acid (DNA) editing agents, and their use in preparing DNA-edited cells and birds. The present disclosure further relates to gene-edited or genetically modified avians and gene-edited or genetically modified avian primordial germ cells (PGCs) for producing gene-edited or genetically modified avians (birds) that can serve as surrogate hosts for donor PGCs. The present disclosure further relates to methods for producing avian strains that can produce viable embryos and offspring, in both sexes, and for their subsequent use as surrogate hosts for donor PGCs.

公开(公告)号：US20230332109A1

公开(公告)日：2023-10-19

申请号：US18028502

申请日：2021-09-24

申请人： Institute of Zoology, Chinese Academy of Sciences ,  Beijing Institute for Stem Cell and Regenerative Medicine

发明人： Lei LI ,  Xiaoxiao WANG ,  Qizhi WANG

IPC分类号： C12N5/074 ,  C12N5/0735 ,  C12N5/0797 ,  C12N5/077 ,  C12N5/071 ,  A61K35/545

CPC分类号： C12N5/0696 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0657 ,  C12N5/0672 ,  A61K35/545 ,  C12N2513/00 ,  C12N2501/115 ,  C12N2501/16 ,  C12N2501/415 ,  C12N2506/45

摘要： A pluripotent stem cell, a method for producing the pluripotent stem cell, and the use of the pluripotent stem cell for stem cell differentiation, cell transplantation, tissue repair, and/or tissue regeneration.

公开(公告)号：US09644178B2

公开(公告)日：2017-05-09

申请号：US14488185

申请日：2014-09-16

申请人： Crystal Bioscience Inc.

发明人： Marie-Cecile van de Lavoir ,  Robert Etches

IPC分类号： C12N5/00 ,  A01K67/027 ,  C12N5/076 ,  C12N5/0735 ,  C12N5/073 ,  C12N5/075 ,  C12N15/85 ,  A61K36/12

CPC分类号： C12N5/061 ,  A01K67/0271 ,  A01K2227/30 ,  A61K35/12 ,  A61K36/12 ,  C12N5/0604 ,  C12N5/0609 ,  C12N5/0611 ,  C12N15/85 ,  C12N2517/02

摘要： A sustained culture of isolated avian gonocytes is provided, as well as a method of making and using the same. A chimeric avian containing an isolated gonocyte and a transgenic avian produced using the chimeric avian are also provided. The cell and method may be employed to make, among other things, transgenic avian that produce a heterologous protein, e.g., a therapeutic protein.

公开(公告)号：US20170086431A1

公开(公告)日：2017-03-30

申请号：US15274201

申请日：2016-09-23

申请人： ALEXION PHARMA LLC

发明人： Marie-Cecile Van De Lavoir ,  Philip A. Leighton

IPC分类号： A01K67/027 ,  C12N15/85 ,  C07K16/00 ,  C12N5/0735

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/15 ,  A01K2227/30 ,  A01K2267/01 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/21 ,  C12N5/0611 ,  C12N5/0697 ,  C12N15/85 ,  C12N15/8509 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2502/11 ,  C12N2502/14 ,  C12N2506/11 ,  C12N2510/00 ,  C12N2517/02 ,  C12N2830/008 ,  C12N2830/40

摘要： The present invention is long-term cultures of avian PGCs and techniques to produce germline chimeric and transgenic birds derived from prolonged PGC cultures. In some embodiments, these PGCs can be transfected with genetic constructs to modify the DNA of the PGC, specifically to introduce a transgene encoding an exogenous protein. When combined with a host avian embryo by known procedures, those modified PGCs are transmitted through the germline to yield transgenic offspring. These germline chimeric birds do not have substantial contributions of PGC-derived phenotypes in somatic cells or tissues. This invention includes compositions comprising long-term cultures of PGCs that can be genetically modified by gene targeting, that can accept large amounts of foreign DNA and that contribute to the germline of recipient embryos.

公开(公告)号：US20160272937A1

公开(公告)日：2016-09-22

申请号：US15030315

申请日：2014-04-14

申请人： THE BRIGHAM AND WOMEN'S HOSPITAL, INC.

发明人： Albert LAM ,  Benjamin FREEDMAN ,  Ryuji MORIZANE ,  Joseph BONVENTRE

IPC分类号： C12N5/0735 ,  A61K35/54

CPC分类号： C12N5/0611 ,  A61K35/54 ,  C12N5/0687 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/724 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2506/45

摘要： Described herein are methods related to generating intermediate mesoderm (IM) cells, including using sequential treatment of small molecules and growth factors, and composition produced by the described methods. Using small molecules such as CHIR99021 in combination with FGF2 and RA, efficient differentiation of human pluripotent stem cells (hPSCs) into intermediate mesoderm, such as PAX2+LHX1+ cells, is achieved. The method is extensible different hPSC cell lines and does not require flow sorting. Importantly, resulting PAX2+LHX1+ cells, are capable of WT1 expression and addition of FGF9 and activin, PAX2+LHX1+ cells specifically differentiates cells into SIX2, SALL1, and WT1 expressing cells representative of cap mesenchyme nephron progenitor cells. The described methods and compositions facilitate and improve the directed differentiation of hPSCs into cells of the kidney lineage for the purposes of bioengineering kidney tissue and iPS cell disease modeling.

摘要翻译： 本文描述了与产生中间胚层（IM）细胞相关的方法，包括使用小分子和生长因子的顺序处理以及由所述方法产生的组合物。 使用小分子如CHIR99021与FGF2和RA组合，实现人多能干细胞（hPSC）到中间胚层如PAX2 + LHX1 +细胞的有效分化。 该方法是可扩展的不同的hPSC细胞系，不需要流分选。 重要的是，所得到的PAX2 + LHX1 +细胞能够进行WT1表达，并加入FGF9和激活素，PAX2 + LHX1 +细胞将细胞特异性分化为表达帽间质肾单核祖细胞的SIX2，SALL1和WT1表达细胞。 所描述的方法和组合物促进和改善hPSC到肾脏谱系细胞的定向分化，用于生物工程肾组织和iPS细胞疾病建模。

公开(公告)号：US20150353887A1

公开(公告)日：2015-12-10

申请号：US14823481

申请日：2015-08-11

申请人： The General Hospital Corporation

发明人： Jonathan Lee Tilly ,  Dori C. Woods

IPC分类号： C12N5/075

CPC分类号： C12N5/0609 ,  C12N5/0611 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/13 ,  C12N2501/235 ,  C12N2502/13 ,  C12N2517/10

摘要： Oogonial stem cell (OSC)-derived compositions, such as nuclear free cytoplasm or isolated mitochondria, and uses of OSC-derived compositions in autologous fertility-enhancing procedures are described.

摘要翻译： 描述了由卵巢干细胞（OSC）衍生的组合物，例如无核细胞质或分离的线粒体，以及在自体生育力增强程序中使用OSC衍生的组合物。

公开(公告)号：US20150104871A1

公开(公告)日：2015-04-16

申请号：US14578443

申请日：2014-12-21

申请人： Technion Research & Development Foundation Limited

发明人： Michal AMIT ,  Joseph Itskovitz-Eldor

IPC分类号： C12N5/074 ,  C12N5/0735

CPC分类号： C12N5/0606 ,  C12N5/0031 ,  C12N5/0037 ,  C12N5/0043 ,  C12N5/0056 ,  C12N5/0607 ,  C12N5/0611 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/235 ,  C12N2501/33 ,  C12N2501/998 ,  C12N2533/52

摘要： The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.

摘要翻译： 本发明是使用无饲养细胞，不含异种植物的培养系统和干细胞来建立和繁殖人类胚胎干细胞系的方法，所述培养系统和干细胞能够在不含异种的培养物中保持未分化，多能和增殖状态 污染物和饲养细胞。