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129 条记录 (耗时 0.039 秒)

    Droplet-Based Pyrosequencing
    101.
    发明申请
    Droplet-Based Pyrosequencing 有权
    基于液滴的焦磷酸测序

    公开(公告)号：US20130164742A1

    公开(公告)日：2013-06-27

    申请号：US13470919

    申请日：2012-05-14

    申请人： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair ,  Peter B. Griffin

    发明人： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair ,  Peter B. Griffin

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

    摘要： The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.

    摘要翻译： 本发明涉及基于液滴的焦磷酸测序，包括鉴定样品核酸中目标位置的碱基的方法。 该方法包括：（a）提供包括包含固定在珠粒上的样品核酸的第一液滴的液滴微致动器; 和（b）在液滴微致动器上：（i）使第一液滴与一个或多个试剂液滴接触以产生第二液滴，其中所述一个或多个试剂液滴包括用于通过掺入来扩展样品核酸的双链部分的试剂 目标位置的核苷酸; （ii）分裂第二液滴以产生包含珠粒的第三液滴和不含珠粒的第四液滴; 和（iii）测定第三液滴以确定核苷酸是否并入目标位置。

    Droplet-based nucleic acid amplification method and apparatus
    105.
    发明申请
    Droplet-based nucleic acid amplification method and apparatus 有权
    基于液滴的核酸扩增方法和装置

    公开(公告)号：US20090291433A1

    公开(公告)日：2009-11-26

    申请号：US11639490

    申请日：2006-12-15

    申请人： Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

    发明人： Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

    IPC分类号： C12Q1/68 ,  G01N21/76 ,  C12P19/34

    CPC分类号： C12Q1/68 ,  B01L7/52 ,  B01L2300/0645 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L2400/0424 ,  B01L2400/0427 ,  C12Q1/6869 ,  G01N27/44791 ,  Y10T436/25 ,  C12Q2565/629 ,  C12Q2565/301 ,  C12Q2563/149

    摘要： The present invention relates to a droplet-based nucleic acid amplification method and apparatus. According to one embodiment, a method of amplifying a nucleic acid in a biological sample is provided, wherein the method includes: (a) providing a system comprising a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator comprising: (i) a sample potentially comprising a target nucleic acid; (ii) a substrate comprising electrodes for conducting droplet operations; and (iii) one or more temperature control means arranged in proximity with one or more of the electrodes for heating a region of the droplet microactuator such that a droplet can be transported into the region for heating; (b) using droplet operations to combine on the droplet microactuator one or more amplification reagent droplets and one or more sample droplets to yield an amplification-ready droplet; and (c) thermal cycling the amplification-ready droplet sufficient to result in amplification of a target nucleic acid when present in the amplification-ready droplet.

    摘要翻译： 本发明涉及一种基于液滴的核酸扩增方法和装置。 根据一个实施方案，提供了扩增生物样品中的核酸的方法，其中所述方法包括：（a）提供包括液滴微致动器的系统，所述液滴微致动器电子耦合到能够执行指令的处理器并由其控制，液滴微致动器 包括：（i）潜在地包含靶核酸的样品; （ii）包括用于进行液滴操作的电极的基板; 和（iii）设置在一个或多个电极附近的一个或多个温度控制装置，用于加热液滴微致动器的区域，使得液滴可以被运送到加热区域中; （b）使用液滴操作在液滴微致动器上组合一个或多个扩增试剂液滴和一个或多个样品液滴以产生扩增就绪液滴; 和（c）将放大准备好的液滴热循环到足以在存在于扩增即用液滴中时导致靶核酸的扩增。

    Integrated droplet actuator for gel; electrophoresis and molecular analysis
    109.
    发明授权
    Integrated droplet actuator for gel; electrophoresis and molecular analysis 有权
    凝胶液滴致动器 电泳和分子分析

    公开(公告)号：US09091649B2

    公开(公告)日：2015-07-28

    申请号：US13508300

    申请日：2010-11-08

    申请人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    发明人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    IPC分类号： G01N27/447 ,  B01L7/00 ,  B01L3/00

    CPC分类号： G01N27/44743 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/046 ,  G01N27/44791

    摘要： The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

    摘要翻译： 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术，液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶，捕获并随后处理电泳凝胶的产物，或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中，在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定，例如通过实时PCR的核酸（例如DNA）定量和一个或多个样品处理操作，例如样品稀释。 在一个实施方案中，电泳凝胶可以集成在液滴致动器的顶部基底上。

    Integrated Droplet Actuator for Gel; Electrophoresis and Molecular Analysis
    110.
    发明申请
    Integrated Droplet Actuator for Gel; Electrophoresis and Molecular Analysis 有权
    集成液滴执行器凝胶; 电泳和分子分析

    公开(公告)号：US20130059366A1

    公开(公告)日：2013-03-07

    申请号：US13508300

    申请日：2010-11-08

    申请人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    发明人： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

    IPC分类号： C12M1/40

    CPC分类号： G01N27/44743 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/046 ,  G01N27/44791

    摘要： The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

    摘要翻译： 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术，液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶，捕获并随后处理电泳凝胶的产物，或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中，在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定，例如通过实时PCR的核酸（例如DNA）定量和一个或多个样品处理操作，例如样品稀释。 在一个实施方案中，电泳凝胶可以集成在液滴致动器的顶部基底上。