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公开(公告)号：US20130293246A1
公开(公告)日：2013-11-07
申请号：US13988190
申请日：2011-11-15
CPC分类号： G01N27/221 , B01L3/502784 , B01L3/502792 , B01L2200/14 , B01L2300/0645 , B01L2300/0816 , B01L2300/089 , B01L2400/0427 , B41J2/04541 , B41J2/04555 , B41J2/0458 , G01K7/34
摘要： The embodiments described herein provide methods of measuring capacitance, detecting a droplet at a position, determining a thickness of an oil film and determining temperature in a droplet actuator. Specifically, the capacitance detection method may be used as a real-time verification tool in order to detect the absence, presence, and/or partial presence of a droplet at an electrode, analyze droplet properties, measure droplet size or volume, optimize the speed of droplet operation and detect air bubbles.
摘要翻译： 本文所述的实施例提供了测量电容，检测位置处的液滴，确定油膜的厚度和确定液滴致动器中的温度的方法。 具体地，可以将电容检测方法用作实时验证工具，以便检测电极处的液滴的不存在，存在和/或部分存在，分析液滴属性，测量液滴尺寸或体积，优化速度 的液滴操作并检测气泡。
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2.发明授权公开(公告)号：US06911132B2
公开(公告)日：2005-06-28
申请号：US10253368
申请日：2002-09-24
IPC分类号： B01F11/00 , B01F13/00 , B01L3/00 , G01N1/00 , G01N1/10 , G01N15/06 , G01N21/00 , G01N35/00 , G01N27/26 , B01D57/00
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0084 , B01L3/00 , B01L3/5027 , B01L3/502784 , B01L3/502792 , B01L2200/06 , B01L2200/0605 , B01L2200/10 , B01L2300/089 , B01L2300/161 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , B41J2002/14395 , C25B9/00 , G01N27/44791 , G02B26/005
摘要： An apparatus is provided for manipulating droplets. The apparatus is a single-sided electrode design in which all conductive elements are contained on one surface on which droplets are manipulated. An additional surface can be provided parallel with the first surface for the purpose of containing the droplets to be manipulated. Droplets are manipulated by performing electrowetting-based techniques in which electrodes contained on or embedded in the first surface are sequentially energized and de-energized in a controlled manner. The apparatus enables a number of droplet manipulation processes, including merging and mixing two droplets together, splitting a droplet into two or more droplets, sampling a continuous liquid flow by forming from the flow individually controllable droplets, and iterative binary or digital mixing of droplets to obtain a desired mixing ratio.
摘要翻译： 提供了一种用于操纵液滴的装置。 该装置是单面电极设计，其中所有导电元件都包含在其上操作液滴的一个表面上。 为了容纳待操作的液滴，可以与第一表面平行地设置另外的表面。 通过执行基于电润湿的技术来操纵液滴，其中包含在或嵌入在第一表面中的电极被顺序地通电并以受控的方式断电。 该装置能够进行多个液滴操纵过程，包括将两个液滴合并和混合在一起，将液滴分成两个或更多个液滴，通过从流动单独控制的液滴形成连续的液体流，以及液滴的迭代二进制或数字混合到 获得所需的混合比。
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公开(公告)号：US07816121B2
公开(公告)日：2010-10-19
申请号：US11639990
申请日：2006-12-15
CPC分类号： B01L3/502761 , B01L3/5025 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0668 , B01L2200/0673 , B01L2200/147 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448 , B01L2400/0487 , G01N15/1484 , G01N2015/1006 , G01N2035/1046 , Y10T29/49002 , Y10T137/0318
摘要： A droplet actuation system is provided including a droplet actuation device including a substrate that includes electrodes for conducting droplet operations; temperature control means for heating and/or cooling a region of the droplet actuation device and arranged such that a droplet can be transported on the electrodes to a region for heating; and a means for effecting a magnetic field in proximity to one or more of the electrodes sufficient to immobilize magnetically responsive beads in a droplet on the substrate during droplet operations. The system further includes a processor for controlling the electrodes and temperature control means, wherein the processor is programmed, and electrodes and magnetic field are arranged, to cause the electrodes to split a droplet including the magnetically responsive beads yielding a first daughter droplet which includes the magnetically responsive beads and a second daughter droplet without a substantial amount of the magnetically responsive beads.
摘要翻译： 提供了一种液滴致动系统，其包括液滴致动装置，其包括包括用于进行液滴操作的电极的基板; 温度控制装置，用于加热和/或冷却液滴致动装置的区域，并布置成使得液滴可以在电极上传送到加热区域; 以及用于在一些或多个电极处进行磁场的装置，其足以在液滴操作期间将磁响应珠固定在基板上的液滴中。 该系统还包括用于控制电极和温度控制装置的处理器，其中处理器被编程，并且布置电极和磁场，以使电极分裂包括磁响应珠的液滴，产生包含第一子液滴的第一子液滴 磁响应珠和第二子液滴，而没有大量的磁响应珠。
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公开(公告)号：US08980198B2
公开(公告)日：2015-03-17
申请号：US11639594
申请日：2006-12-15
IPC分类号： B01L3/00 , G01N27/447 , B01F13/00 , B01L7/00 , G01N35/10
CPC分类号： B01L3/502792 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , B03C5/02 , B81B1/00 , G01N27/447 , G01N2035/1046
摘要： The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.
摘要翻译： 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了液滴微致动器，其包括：（a）第一基板，其包括被配置用于在所述基板的表面上进行液滴操作的电极; （b）第二衬底，其与衬底的表面间隔足以限定第一衬底和第二衬底之间的内部空间的距离，其中所述距离足以容纳设置在第一衬底上的空间中的液滴; 和（c）布置在内部体积中并且以允许使用电极对液滴进行液滴操作的方式相对于电极布置的液滴。
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公开(公告)号：US20110100823A1
公开(公告)日：2011-05-05
申请号：US12960583
申请日：2010-12-06
IPC分类号： G01N21/76 , G01N27/447
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet-based nucleic acid amplification apparatus and system. According to one embodiment, a droplet microactuator is provided made using a first substrate including a fluorescing material and including a detection region for detecting a fluorescence signal from a droplet, which detection region is coated with a light absorbing, low fluorescence or non-fluorescing material.
摘要翻译： 本发明涉及一种基于液滴的核酸扩增装置和系统。 根据一个实施例，使用包括荧光材料的第一基底并且包括用于检测来自液滴的荧光信号的检测区域来提供液滴微致动器，该检测区域被光吸收，低荧光或非荧光材料涂覆 。
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公开(公告)号：US07329545B2
公开(公告)日：2008-02-12
申请号：US10253342
申请日：2002-09-24
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： In a method for sampling a continuous liquid flow, the liquid flow is supplied to a surface along an input flow path. The liquid flow is sampled by forming a sample droplet from a portion of the liquid flow. The sample droplet is moved along an analysis flow path to a processing area of the surface, where the sample droplet is processed. Discrete sample droplets are formed and moved using an electrowetting technique. A binary mixing apparatus and method are also provided. The apparatus comprises an array of electrodes, an electronic controller communicating with the electrodes, a sample droplet supply area communicating with the array, and an additive droplet supply area communicating with the array. The electronic controller alternately energizes and de-energizes selected electrodes to carry out droplet-to-droplet binary mixing operations to obtain one or more droplets having a target mixing ratio.
摘要翻译： 在对连续液体流进行取样的方法中，液体流被输送到沿输入流路的表面。 通过从液体流的一部分形成样品液滴来取样液体流。 样品液滴沿着分析流动路径移动到表面的处理区域，在该处理区域处理样品液滴。 使用电润湿技术形成并移动离散样品液滴。 还提供了二元混合装置和方法。 该装置包括电极阵列，与电极连通的电子控制器，与该阵列连通的样品液滴供应区域以及与该阵列连通的附加液滴供应区域。 电子控制器交替地激励和去激活所选择的电极以进行液滴到液滴的二进制混合操作以获得具有目标混合比的一个或多个液滴。
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公开(公告)号：US20070242105A1
公开(公告)日：2007-10-18
申请号：US11639594
申请日：2006-12-15
IPC分类号： B41J2/045
CPC分类号： B01L3/502792 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L7/54 , B01L2200/0647 , B01L2200/0673 , B01L2200/10 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/089 , B01L2300/1827 , B01L2400/0427 , B01L2400/0448 , B03C5/02 , B81B1/00 , G01N27/447 , G01N2035/1046
摘要： The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.
摘要翻译： 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了液滴微致动器，其包括：（a）第一基板，其包括被配置用于在所述基板的表面上进行液滴操作的电极; （b）第二衬底，其与衬底的表面间隔足以限定第一衬底和第二衬底之间的内部空间的距离，其中所述距离足以容纳设置在第一衬底上的空间中的液滴; 和（c）布置在内部体积中并且以允许使用电极对液滴进行液滴操作的方式相对于电极布置的液滴。
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公开(公告)号：US08872527B2
公开(公告)日：2014-10-28
申请号：US12527208
申请日：2008-02-15
CPC分类号： B01L3/50273 , B01L3/502715 , B01L3/502784 , B01L2200/0605 , B01L2300/0627 , B01L2300/0636 , B01L2300/0645 , B01L2300/0809 , B01L2400/0427 , G01N27/22 , G01N27/223 , G01N27/227 , G01N2203/00 , G01V3/02
摘要： A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.
摘要翻译： 一种用于检测液滴致动器上电容的方法，电路和装置，特别是用于通过以下步骤确定液滴致动器上的电极处的液滴的存在，部分存在或不存在：（a）提供液滴致动器，其包括：（i） 基板，其包括布置在所述基板上的电极，用于在所述基板的表面上进行液滴操作; （ii）电容检测电路，用于检测一个或多个电极处的液滴操作表面处的电容; （b）在一个或多个电极处检测液滴操作表面处的电容; 和（c）根据电容确定在电极处的液滴操作表面处的液滴的存在，部分存在或不存在。
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公开(公告)号：US07815871B2
公开(公告)日：2010-10-19
申请号：US11639664
申请日：2006-12-15
CPC分类号： B41J2/125 , B01L3/502792 , B01L2200/143 , B01L2200/147 , B01L2300/0645 , B01L2300/0654 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0487 , B41J2/17509 , G01N15/1484 , G01N2015/1006
摘要： The present invention relates to a droplet microactuator system. According to one embodiment, the droplet microactuator system includes: (a) a droplet microactuator configured to conduct droplet operations; (b) a magnetic field source arranged to immobilize magnetically responsive beads in a droplet during droplet operations; (c) a sensor configured in a sensing relationship with the droplet microactuator, such that the sensor is capable of sensing a signal from and/or a property of one or more droplets on the droplet microactuator; and (d) one or more processors electronically coupled to the droplet microactuator and programmed to control electrowetting-mediated droplet operations on the droplet actuator and process electronic signals from the sensor.
摘要翻译： 液滴微致动器系统技术领域本发明涉及液滴微致动器系统。 根据一个实施例，液滴微致动器系统包括：（a）构造成进行液滴操作的液滴微致动器; （b）磁场源，布置成在液滴操作期间将磁响应珠固定在液滴中; （c）配置成与液滴微致动器的感测关系的传感器，使得传感器能够感测来自液滴微致动器上的一个或多个液滴的信号和/或特性; 和（d）电子耦合到液滴微致动器的一个或多个处理器，并被编程为控制液滴致动器上的电润湿介导的液滴操作并处理来自传感器的电子信号。
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公开(公告)号：US20090291433A1
公开(公告)日：2009-11-26
申请号：US11639490
申请日：2006-12-15
CPC分类号： C12Q1/68 , B01L7/52 , B01L2300/0645 , B01L2300/1822 , B01L2300/1894 , B01L2400/0424 , B01L2400/0427 , C12Q1/6869 , G01N27/44791 , Y10T436/25 , C12Q2565/629 , C12Q2565/301 , C12Q2563/149
摘要： The present invention relates to a droplet-based nucleic acid amplification method and apparatus. According to one embodiment, a method of amplifying a nucleic acid in a biological sample is provided, wherein the method includes: (a) providing a system comprising a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator comprising: (i) a sample potentially comprising a target nucleic acid; (ii) a substrate comprising electrodes for conducting droplet operations; and (iii) one or more temperature control means arranged in proximity with one or more of the electrodes for heating a region of the droplet microactuator such that a droplet can be transported into the region for heating; (b) using droplet operations to combine on the droplet microactuator one or more amplification reagent droplets and one or more sample droplets to yield an amplification-ready droplet; and (c) thermal cycling the amplification-ready droplet sufficient to result in amplification of a target nucleic acid when present in the amplification-ready droplet.
摘要翻译： 本发明涉及一种基于液滴的核酸扩增方法和装置。 根据一个实施方案，提供了扩增生物样品中的核酸的方法，其中所述方法包括：（a）提供包括液滴微致动器的系统，所述液滴微致动器电子耦合到能够执行指令的处理器并由其控制，液滴微致动器 包括：（i）潜在地包含靶核酸的样品; （ii）包括用于进行液滴操作的电极的基板; 和（iii）设置在一个或多个电极附近的一个或多个温度控制装置，用于加热液滴微致动器的区域，使得液滴可以被运送到加热区域中; （b）使用液滴操作在液滴微致动器上组合一个或多个扩增试剂液滴和一个或多个样品液滴以产生扩增就绪液滴; 和（c）将放大准备好的液滴热循环到足以在存在于扩增即用液滴中时导致靶核酸的扩增。
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