公开(公告)号：US20160245774A1

公开(公告)日：2016-08-25

申请号：US15144591

申请日：2016-05-02

Applicant: Noureddine Tayebi ,  Xing Su ,  Handong Li

Inventor： Noureddine Tayebi ,  Xing Su ,  Handong Li

IPC: G01N27/327 ,  C23C16/00

CPC classification number: G01N27/3278 ,  C23C16/00

Abstract: Highly selective coated-electrode nanogap transducers for the detection of redox molecules are described. In an example, an analyte detection system includes one or more transducer electrodes having a surface for analyte detection. The surface includes a coating to inhibit direct contact of analyte with the surface of the one or more transducer electrodes.

Abstract translation: 描述了用于检测氧化还原分子的高选择性涂层电极纳米胶片转换器。 在一个示例中，分析物检测系统包括具有用于分析物检测的表面的一个或多个换能器电极。 表面包括抑制分析物与一个或多个换能器电极的表面的直接接触的涂层。

公开(公告)号：US20150184237A1

公开(公告)日：2015-07-02

申请号：US14142603

申请日：2013-12-27

Applicant: XING SU ,  Mark Oldham

Inventor： XING SU ,  Mark Oldham

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  G01N27/00 ,  C12Q2521/101 ,  C12Q2521/543 ,  C12Q2537/157 ,  C12Q2563/116 ,  C12Q2565/607

Abstract: Devices and methods for detecting, identifying, and sequencing, compounds, complexes, and molecules are described. Electronic detection is combined with optical excitation to determine the presence or identity of an analyte of interest. Embodiments of the invention additionally provide devices and methods that allow highly parallel nucleic acid sequence determination.

Abstract translation: 描述了用于检测，鉴定和测序化合物，复合物和分子的装置和方法。 电子检测与光激发组合以确定感兴趣的分析物的存在或身份。 本发明的实施方案另外提供允许高度平行核酸序列测定的装置和方法。

公开(公告)号：US08962279B2

公开(公告)日：2015-02-24

申请号：US12655459

申请日：2009-12-30

Applicant: David J. Liu ,  Xing Su ,  Kai Wu

Inventor： David J. Liu ,  Xing Su ,  Kai Wu

IPC: C12P19/34 ,  C12M1/00 ,  C12Q1/48 ,  C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/48 ,  C12Q1/6837 ,  C12Q1/6869 ,  G01N27/4145 ,  G01N2333/9125 ,  C12Q2565/607 ,  C12Q2565/507 ,  C12Q2565/301

Abstract: Methods for sequencing nucleic acids are presented. Sequencing is accomplished through the chemical amplification of the products of DNA synthesis and the detection of the chemically amplified products. In embodiments of the invention, a substrate is provided having a plurality of molecules of DNA to be sequenced attached and a plurality of molecules capable of chelating pyrophosphate ions attached, the DNA molecules to be sequenced are primed, and a next complementary nucleotide is incorporated and excised a plurality of times leading to the buildup of pyrophosphate ions locally around the DNA molecule to be sequenced. Pyrophosphate ions are captured by the substrate-attached chelators and electronically detected to determine the identity of the next complementary nucleic acid in the DNA molecule to be sequenced. Additionally, devices and methods are provided for detecting biomolecules through the detection of pyrophosphate ions.

Abstract translation: 介绍了核酸测序方法。 通过化学扩增DNA合成产物和化学扩增产物的检测来完成测序。 在本发明的实施方案中，提供了具有多个待测序的DNA分子的底物和能够螯合连接的焦磷酸根离子的多个分子，引入待测序的DNA分子，并且掺入下一个互补核苷酸， 切除多次导致待测序的DNA分子局部周围的焦磷酸盐离子的积聚。 焦磷酸根离子被底物连接的螯合剂捕获并电子检测以确定待测序的DNA分子中下一个互补核酸的同一性。 另外，提供了通过检测焦磷酸根离子来检测生物分子的装置和方法。

公开(公告)号：US20140342470A1

公开(公告)日：2014-11-20

申请号：US14448141

申请日：2014-07-31

Applicant: Xing Su ,  David J. Liu ,  Kai Wu ,  Kenneth B. Swartz ,  Mineo Yamakawa

Inventor： Xing Su ,  David J. Liu ,  Kai Wu ,  Kenneth B. Swartz ,  Mineo Yamakawa

IPC: G01N1/40

CPC classification number: G01N1/4044 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L2400/0638 ,  B01L2400/086 ,  G01N35/0098 ,  G01N35/08 ,  G01N2015/0038 ,  G01N2015/0092 ,  H01F7/20 ,  H01F7/206 ,  H01F2007/068 ,  Y10S436/805 ,  Y10S436/806 ,  Y10T29/49117 ,  Y10T29/4913 ,  Y10T29/49155 ,  Y10T436/25

Abstract: An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

Abstract translation: 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性颗粒和/或信号颗粒。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

公开(公告)号：US20140299485A1

公开(公告)日：2014-10-09

申请号：US14267327

申请日：2014-05-01

Applicant: Jonathan S. Daniels ,  Oguz H. Elibol ,  Grace M. Credo ,  Xing Su

Inventor： Jonathan S. Daniels ,  Oguz H. Elibol ,  Grace M. Credo ,  Xing Su

IPC: G01N27/22

CPC classification number: G01N27/3277 ,  B82Y30/00 ,  C12Q1/68 ,  G01D5/24 ,  G01K7/34 ,  G01L1/14 ,  G01N27/22 ,  G01R27/26 ,  G06K7/08

Abstract: Described herein is a device comprising a plurality of first reaction electrodes arranged in an array, the plurality of first reaction electrodes configured to be exposed to a solution and having a capacitance; first circuitry configured to controllably connect the plurality of first reaction electrodes to a bias source and controllably disconnect the plurality of first reaction electrodes from the bias source; and second circuitry configured to measure a rate of charging or discharging of the capacitance. Also described herein is a method of using this device to sequence DNA.

Abstract translation: 这里描述的是一种装置，其包括排列成阵列的多个第一反应电极，多个第一反应电极构造为暴露于溶液并具有电容; 第一电路，被配置为将多个第一反应电极可控地连接到偏置源并且可控制地将多个第一反应电极与偏置源分离; 以及第二电路，被配置为测量电容的充电或放电速率。 本文还描述了使用该装置来对DNA进行序列化的方法。

公开(公告)号：US08715932B2

公开(公告)日：2014-05-06

申请号：US12860462

申请日：2010-08-20

Applicant: Xing Su ,  Kai Wu ,  Liming Wang ,  Jianquan Liu ,  Grace M. Credo

Inventor： Xing Su ,  Kai Wu ,  Liming Wang ,  Jianquan Liu ,  Grace M. Credo

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q2531/125 ,  C12Q2535/122 ,  C12Q2565/301 ,  C12Q2565/607

Abstract: Nucleic acid sequencing using concatemers of DNA is provided. Optionally, amplified reaction products from the repeated incorporation and excision of a nucleoside complementary to a nucleoside of the DNA to be sequenced onto primer molecules hybridized to the concatemers of DNA are detected. Nucleic acid sequencing using concatemers of DNA and non-natural oligonucleotides is also provided. Nucleic acid sequencing reactions are detected electronically and or optically using arrays of detectors.

Abstract translation: 提供使用DNA连接子的核酸测序。 任选地，检测来自重复掺入和切除与待测序的DNA的核苷互补的核苷酸的扩增反应产物与DNA连接体杂交的引物分子上。 还提供了使用DNA和非天然寡核苷酸的并联体的核酸测序。 使用检测器阵列电子和/或光学检测核酸测序反应。

公开(公告)号：US08674086B2

公开(公告)日：2014-03-18

申请号：US12823995

申请日：2010-06-25

Applicant: Jianquan Liu ,  Xing Su ,  Kai Wu

Inventor： Jianquan Liu ,  Xing Su ,  Kai Wu

IPC: C07H19/20 ,  C07H19/00 ,  C07H19/04

CPC classification number: C07H19/10 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2521/319 ,  C12Q2521/525 ,  C12Q2525/186 ,  C12Q2537/157 ,  C12Q2565/537 ,  C12Q2565/607

Abstract: Embodiments of the invention provide non-natural bifunctional nucleotides having both nuclease resistance and nucleic acid synthesis blocking properties and methods of sequencing nucleic acids that employ non-natural bifunctional nucleic acids. Additional embodiments provide non-natural oligonucleotides and methods for sequencing nucleic acids using the non-natural oligonucleotides. Methods according to embodiments of the invention employ electronic detection and fluorescent detection of nucleic acid sequencing reactions.

Abstract translation: 本发明的实施方案提供具有核酸酶抗性和核酸合成阻断性质的非天然双功能核苷酸，以及使用非天然双功能核酸测序核酸的方法。 另外的实施方案提供非天然寡核苷酸和使用非天然寡核苷酸测序核酸的方法。 根据本发明的实施方案的方法使用核酸测序反应的电子检测和荧光检测。

公开(公告)号：US20140001055A1

公开(公告)日：2014-01-02

申请号：US13538346

申请日：2012-06-29

Applicant: Oguz H. Elibol ,  Grace M. Credo ,  Xing Su ,  Madoo Varma ,  Jonathan S. Daniels ,  Drew Hall ,  Handong Li ,  Noureddine Tayebi ,  Kai Wu

Inventor： Oguz H. Elibol ,  Grace M. Credo ,  Xing Su ,  Madoo Varma ,  Jonathan S. Daniels ,  Drew Hall ,  Handong Li ,  Noureddine Tayebi ,  Kai Wu

IPC: G01N27/26 ,  B82Y5/00 ,  B82Y30/00

CPC classification number: G01N27/3277 ,  B01J19/0046 ,  B01J2219/00608 ,  B01J2219/00653 ,  B01J2219/00722 ,  B81B7/04 ,  B81B2201/0214 ,  B81B2201/058 ,  B82Y15/00 ,  C40B20/04 ,  C40B50/16 ,  G01N27/3278 ,  G01N27/4145 ,  G01N27/4146 ,  G01N27/4148

Abstract: Various embodiments provide devices, methods, and systems for high throughput biomolecule detection using transducer arrays. In one embodiment, a transducer array made up of transducer elements may be used to detect byproducts from chemical reactions that involve redox genic tags. Each transducer element may include at least a reaction chamber and a fingerprinting region, configured to flow a fluid from the reaction chamber through the fingerprinting region. The reaction chamber can include a molecule attachment region and the fingerprinting region can include at least one set of electrodes separated by a nanogap for conducting redox cycling reactions. In embodiments, by flowing the chamber content obtained from a reaction of a latent redox tagged probe molecule, a catalyst, and a target molecule in the reaction chamber through the fingerprinting region, the redox cycling reactions can be detected to identify redox-tagged biomolecules.

Abstract translation: 各种实施方案提供使用换能器阵列进行高通量生物分子检测的装置，方法和系统。 在一个实施例中，由换能器元件组成的换能器阵列可用于检测涉及氧化还原基因标签的化学反应的副产物。 每个换能器元件可以至少包括反应室和指纹区域，其被配置为使来自反应室的流体通过指纹区域流动。 反应室可以包括分子附着区域，并且指纹区域可以包括由纳米间隙分开的至少一组电极，用于进行氧化还原循环反应。 在实施方案中，通过使反应室中潜氧氧化还原标记的探针分子，催化剂和靶分子的反应通过指纹区域流动所述腔室内容物，可以检测氧化还原循环反应以鉴定氧化还原标记的生物分子。

公开(公告)号：US08501245B2

公开(公告)日：2013-08-06

申请号：US10808872

申请日：2004-03-24

Applicant: Mian Ying Wang ,  Chen Xing Su ,  Afa Kehaati Palu ,  Bing-Nan Zhou ,  Brett Justin West ,  Johannes Joseph Westendorf ,  Claude Jarakae Jensen ,  Stephen Paul Story

Inventor： Mian Ying Wang ,  Chen Xing Su ,  Afa Kehaati Palu ,  Bing-Nan Zhou ,  Brett Justin West ,  Johannes Joseph Westendorf ,  Claude Jarakae Jensen ,  Stephen Paul Story

IPC: A61K36/00

CPC classification number: A61K36/746 ,  A61K31/353

Abstract: Implementation is directed to selectively inhibiting estrogen production and providing estrogenic effects in a human body. A dietary supplement includes a processed Morinda citrifolia product that is used to inhibit aromatase or aromatase enzymes that function to convert androgens to estrogens, inhibit receptors from binding with estrogen, and reduce and/or regulate estrogen production, as well as reduce the amount of estrogen produced within the body and regulating such production. The dietary supplement further provides estrogenic effects. The present invention methods and compositions effectively function to treat estrogen-dependent cancers, and particularly inhibit, destroy, and reverse the effects of of estrogen-dependent cancerous tumors through the introduction into the body (e.g. ingesting) a safe, pre-determined dosage of a naturaceutical composition formulated with or comprising one or more processed Morinda citrifolia products for a safe, pre-determined duration.

Abstract translation: 实施的目的是选择性地抑制雌激素产生并在人体内提供雌激素作用。 膳食补充剂包括加工的Morinda citrifolia产品，其用于抑制将雄激素转化成雌激素，抑制受体与雌激素结合的芳香酶或芳香酶，以及减少和/或调节雌激素的产生以及减少雌激素的量 在体内生产并调节这种生产。 膳食补充剂进一步提供雌激素作用。 本发明的方法和组合物有效地用于治疗雌激素依赖性癌症，特别是抑制，破坏和逆转雌激素依赖性癌性肿瘤的作用，通过引入体内（例如摄取）安全的预定剂量 配制或包含一种或多种加工的Morinda citrifolia产品的保健组合物用于安全的预定持续时间。

公开(公告)号：US08438099B2

公开(公告)日：2013-05-07

申请号：US12914639

申请日：2010-10-28

Applicant: Richard Co ,  TuenTuen Wang ,  Xing Su ,  John Labuszewski

Inventor： Richard Co ,  TuenTuen Wang ,  Xing Su ,  John Labuszewski

IPC: G06Q40/00

CPC classification number: G06Q40/04 ,  G06Q40/06

Abstract: A periodic reset total return index may be based on a standard index, such as an equity index. The value of the periodic reset total return index may be the sum of the standard index plus the income flow generated by the index, such as dividends generated by stocks. The periodic reset total return index valuation may be deployed as the basis for a futures contract. On a periodic basis, the income flow accrued for the preceding period are passed from the short to the long position holder, with a corresponding adjustment of the settlement price of the contract. The expiration of the contract may be settled at the sum of the underlying index quotation plus the income flow accrual for the previous period. A buyer of a futures contract based on a periodic reset total return index receives the performance of the index plus the intervening income flow accrual.

Abstract translation: 定期复位总回报指数可以基于标准指数，如权益指数。 定期复位总回报指数的价值可以是标准指数加上指数产生的收入流量之和，如股票产生的股息。 周期性复位总回报指数估值可以部署为期货合约的基础。 定期按照上期计入当期损益，由短仓转为长期持有人，并对合同结算价格进行相应调整。 合同到期可以按照相关指数报价加上一个期间的应计利润总额来结算。 根据定期重置总回报指数的期货合约的买方收到指数的表现加上间接收入流动应计利润。