公开(公告)号：US20180135082A1

公开(公告)日：2018-05-17

申请号：US15564327

申请日：2016-04-07

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Nicholas Eakley ,  Kevin Gregory Hoff ,  Robin E. Osterhout ,  Priti Pharkya ,  Erik C. Ralph ,  Stephen Van Dien

IPC: C12P5/02 ,  C12P7/16 ,  C12N9/04 ,  C12N9/02

CPC classification number: C12P5/026 ,  C12N9/0006 ,  C12N9/001 ,  C12P7/04 ,  C12P7/16 ,  Y02E50/10

Abstract: Disclosed are methods and engineered microorganisms that enhance or improve the production of crotyl alcohol. The engineered microorganisms include genetic modifications in alcohol dehydrogenase, alkene reductase or both enzymatic activities. By such genetic modifications, a crotyl alcohol production pathway is provided or improved.

公开(公告)号：US09657316B2

公开(公告)日：2017-05-23

申请号：US13975678

申请日：2013-08-26

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/24 ,  C12P7/28 ,  C12P7/18 ,  C12N1/20 ,  C12N1/00 ,  C07H21/04 ,  C08G18/00 ,  C08G63/00 ,  C08G63/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07D207/12 ,  C08G18/32 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P19/02

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：US09556461B2

公开(公告)日：2017-01-31

申请号：US14226631

申请日：2014-03-26

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Priti Pharkya ,  Mark J. Burk

IPC: C12N1/00 ,  C12N1/26 ,  C12N9/10 ,  C12P7/00 ,  C12P7/62 ,  C12N15/52 ,  C12P5/00 ,  C12P5/02 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00 ,  A61K38/00

CPC classification number: C12P7/62 ,  C12N15/52 ,  C12P5/005 ,  C12P5/026 ,  C12P7/16 ,  C12P7/40 ,  C12P9/00 ,  Y02E50/10

Abstract: The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.

Abstract translation: 本发明提供了具有甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯途径。 本发明另外提供了使用这些生物体来生产甲苯，苯，对甲苯甲酸酯，对苯二甲酸酯，（2-羟基-3-甲基-4-氧代丁氧基）膦酸酯，（2-羟基-4-氧代丁氧基）膦酸酯，苯甲酸酯，苯乙烯 ，2,4-戊二烯酸酯，3-丁烯-1醇或1,3-丁二烯。

公开(公告)号：US09458480B2

公开(公告)日：2016-10-04

申请号：US13730612

申请日：2012-12-28

Applicant: GENOMATICA, INC.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C08G69/10 ,  C12P17/12 ,  C12P13/00 ,  C12N1/00 ,  C12N1/20 ,  C08G69/26 ,  C08G73/00 ,  C07C205/00 ,  C07C211/00 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/02 ,  C12P17/10

CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US09382556B2

公开(公告)日：2016-07-05

申请号：US14450150

申请日：2014-08-01

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

IPC: C12P7/46 ,  C12P7/44 ,  C12P13/00 ,  C12P13/02 ,  C12P17/10 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/80 ,  C12N9/88

CPC classification number: C12N15/52 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/80 ,  C12N9/88 ,  C12P7/44 ,  C12P7/46 ,  C12P7/62 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/10 ,  C12Y101/01035 ,  C12Y102/0101 ,  C12Y103/01031 ,  C12Y206/01 ,  C12Y402/01

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

公开(公告)号：US09321701B2

公开(公告)日：2016-04-26

申请号：US13365132

申请日：2012-02-02

Applicant: Mark J. Burk ,  Anthony P. Burgard ,  Jun Sun ,  Robin E. Osterhout ,  Priti Pharkya

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Jun Sun ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P5/02 ,  C12N1/20 ,  C12N1/00 ,  C07H21/04 ,  C07C1/24 ,  C12P7/04

CPC classification number: C12P5/02 ,  C07C1/24 ,  C07C2521/04 ,  C07C2521/12 ,  C07C2521/16 ,  C07C2523/10 ,  C07C2523/28 ,  C07C2523/31 ,  C07C2523/745 ,  C07C2527/167 ,  C07C2527/173 ,  C12N15/52 ,  C12P5/026 ,  C12P7/04 ,  Y02E50/343 ,  C07C11/167

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene pathway. The invention additionally provides methods of using such organisms to produce butadiene.

公开(公告)号：US09169486B2

公开(公告)日：2015-10-27

申请号：US13527440

申请日：2012-06-19

Applicant: Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12N1/20 ,  C12P5/02 ,  C12N15/52 ,  C12P7/04 ,  C12P7/16

CPC classification number: C12P7/04 ,  C12N15/52 ,  C12N15/70 ,  C12P5/026 ,  C12P7/16

Abstract: The invention provides non-naturally occurring microbial organisms having a butadiene or crotyl alcohol pathway. The invention additionally provides methods of using such organisms to produce butadiene or crotyl alcohol.

Abstract translation: 本发明提供具有丁二烯或巴豆醇途径的非天然存在的微生物。 本发明另外提供了使用这种生物体来生产丁二烯或巴豆醇的方法。

公开(公告)号：US09133487B2

公开(公告)日：2015-09-15

申请号：US13436811

申请日：2012-03-30

Applicant: Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Jun Sun ,  Priti Pharkya

IPC: C12N1/12 ,  C12N1/20 ,  C12P7/40 ,  C12P19/32 ,  C12P7/42 ,  C12P7/62

CPC classification number: C12P7/40 ,  C12P7/42 ,  C12P7/62 ,  C12P19/32

Abstract: The invention provides a non-naturally occurring microbial organism having a methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a methacrylic acid pathway. The invention additionally provides a method for producing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate. The method can include culturing methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme in a sufficient amount to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate, under conditions and for a sufficient period of time to produce methacrylic acid, methacrylate ester, 3-hydroxyisobutyrate and/or 2-hydroxyisobutyrate.

Abstract translation: 本发明提供具有甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯途径的非天然存在的微生物生物。 微生物生物体含有编码甲基丙烯酸途径中的酶的至少一种外源核酸。 本发明另外提供了生产甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的方法。 该方法可以包括培养产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯的微生物，其中微生物生物体表达至少一种编码甲基丙烯酸途径酶的外源核酸，其量足以产生甲基丙烯酸， 甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯在条件下和足够的时间以产生甲基丙烯酸，甲基丙烯酸酯，3-羟基异丁酸酯和/或2-羟基异丁酸酯。

公开(公告)号：US09096873B2

公开(公告)日：2015-08-04

申请号：US12921547

申请日：2009-03-11

Applicant: Liang Wu ,  Axel Christoph Trefzer ,  Stefaan Marie Andre De Wildeman ,  Marco Alexander Van Den Berg

Inventor： Liang Wu ,  Axel Christoph Trefzer ,  Stefaan Marie Andre De Wildeman ,  Marco Alexander Van Den Berg

IPC: C12P7/44 ,  C12N15/70

CPC classification number: C12P7/44 ,  C12N15/70 ,  C12P7/62 ,  C12P17/10

Abstract: The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid (6-ACA), a method for preparing 5-formyl valeric acid (5-FVA), and a method for preparing caprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

Abstract translation: 本发明涉及一种制备己二酸酯或硫酯的方法。 本发明还涉及从所述酯或硫酯制备己二酸的方法。 此外，本发明提供了许多用于制备所述酯或硫酯的中间体的方法。 本发明还涉及制备6-氨基己酸（6-ACA）的方法，5-甲酰戊酸（5-FVA）的制备方法和己内酰胺的制备方法。 此外，本发明涉及用于根据本发明的方法的宿主细胞。

公开(公告)号：US08993285B2

公开(公告)日：2015-03-31

申请号：US12772094

申请日：2010-04-30

Applicant: Anthony P. Burgard

Inventor： Anthony P. Burgard

IPC: C12P7/16 ,  C12N1/21 ,  C12P17/16 ,  C12N9/04 ,  C12N9/10 ,  C12N9/16 ,  C12N9/88 ,  C12N9/00 ,  C12P7/04

CPC classification number: C12P17/16 ,  C12N9/0006 ,  C12N9/1025 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04

Abstract: A non-naturally occurring microbial organism having an isopropanol pathway includes at least one exogenous nucleic acid encoding an isopropanol pathway enzyme. The pathway includes an enzyme selected from a 4-hydroxybutyryl-CoA dehydratase, a crotonase, a 3-hydroxybutyryl-CoA dehydrogenase, an acetoacetyl-CoA synthetase, an acetyl-CoA:acetoacetate-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetate decarboxylase, and an acetone reductase. A non-naturally occurring microbial organism having an n-butanol pathway includes at least one exogenous nucleic acid encoding an n-butanol pathway enzyme. Other non-naturally occurring microbial organism have n-butanol or isobutanol pathways. The organisms are cultured to produce isopropanol, n-butanol, or isobutanol.

Abstract translation: 具有异丙醇途径的非天然存在的微生物生物体包括至少一种编码异丙醇途径酶的外源核酸。 该途径包括选自4-羟基丁酰辅酶A脱水酶，巴豆酸酶，3-羟基丁酰辅酶A脱氢酶，乙酰乙酰辅酶A合成酶，乙酰辅酶A：乙酰乙酸酯-CoA转移酶，乙酰乙酰辅酶A水解酶，乙酰乙酸酯 脱羧酶和丙酮还原酶。 具有正丁醇途径的非天然存在的微生物包括至少一种编码正丁醇途径酶的外源核酸。 其他非天然存在的微生物生物体具有正丁醇或异丁醇途径。 培养生物体以产生异丙醇，正丁醇或异丁醇。