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公开(公告)号：US20230357711A1
公开(公告)日：2023-11-09
申请号：US18026474
申请日：2021-03-23
申请人： Konica Minolta, Inc.
发明人： TEMMEI ITO , AKIHIRO MAEZAWA , YASUHIKO TABATA , YUKI MURATA
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12N2533/30 , C12N2529/00 , C12N2533/54
摘要： The present invention relates to a cell support including a base material containing a biocompatible substance or a base material having a surface to which the biocompatible substance is imparted, and gelatin particles held by the biocompatible substance of the base material. The cell support can be produced by a method of causing the base material containing a biocompatible substance to hold the gelatin particles. By seeding cells on the cell support and culturing the cells, a cell structure in which a reagent or a drug has been uniformly taken into more cells can be obtained.
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公开(公告)号：US11746328B2
公开(公告)日：2023-09-05
申请号：US16490302
申请日：2018-03-03
发明人： Xiaoyu Zhang , Yama A. Abassi , Nan Li , Xiaobo Wang
CPC分类号： C12N5/0657 , C12N5/0018 , C12N5/0696 , C12N13/00 , G01N27/028 , C12N2506/45 , C12N2529/00
摘要： Methods of inducing functional maturation of immature cardiomyocytes derived from induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) by electrically pacing the immature cardiomyocytes according to a pulse profile that induces maturation until the immature cardiomyocytes mature into functionally adult cardiomyocytes.
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公开(公告)号：US11717539B2
公开(公告)日：2023-08-08
申请号：US16576676
申请日：2019-09-19
发明人： Shai Novik , Dror Mevorach
IPC分类号： A61K35/17 , A61K45/06 , A61K39/00 , A61K39/395 , C07K16/28 , C12N5/0783
CPC分类号： A61K35/17 , A61K39/0011 , A61K39/39541 , A61K39/39558 , A61K45/06 , C07K16/2887 , C12N5/0636 , A61K2039/505 , A61K2039/5158 , C12N2501/04 , C12N2501/91 , C12N2523/00 , C12N2529/00 , A61K39/39558 , A61K2300/00
摘要： Compositions disclosed herein, and methods of use thereof included those for inhibiting or reducing the incidence of cytokine release syndrome or cytokine storm in a subject undergoing CAR T-cell therapy, methods of treating a cancer or tumor, methods of reducing tumor load, methods of reducing the size or growth rate of a cancer or a tumor, and methods of extending of the survival of a subject suffering from a cancer or tumor, wherein the subjects are administered compositions comprising apoptotic cells or apoptotic cell supernatants. Compositions and methods of use thereof may increase the efficacy of a CAR T-cell cancer therapy. Disclosed herein are also compositions and methods of use thereof for decreasing or inhibiting cytokine production in a subject experiencing cytokine release syndrome or cytokine storm. In certain instances compositions may include additional chemotherapeutic or immunomodulatory agents.
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公开(公告)号：US20180265858A1
公开(公告)日：2018-09-20
申请号：US15912165
申请日：2018-03-05
发明人： Glauco R. SOUZA , Renata Pasqualini , Wadih ARAP , Thomas Charles KILLIAN , Robert M. RAPHAEL , Daniel Joshua STARK
CPC分类号： C12N13/00 , B82Y5/00 , C12M35/06 , C12N5/0062 , C12N2529/00 , C12N2533/10
摘要： Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.
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公开(公告)号：US10034738B2
公开(公告)日：2018-07-31
申请号：US14650227
申请日：2013-12-06
CPC分类号： A61F2/02 , A61F2230/0002 , A61F2240/002 , A61L27/24 , A61L27/3886 , A61L2430/20 , C12M21/08 , C12M25/00 , C12M35/04 , C12N5/0657 , C12N5/0697 , C12N2506/02 , C12N2513/00 , C12N2529/00 , C12N2533/54
摘要： Methods and devices are provided for the formation of cardiac tissue constructs. In some embodiments, methods are provided for forming cardiac tissue constructs that including cardiomyocytes, non-myocytes, and extracellular matrix, and which exhibit properties associated with healthy cardiac tissue. In some embodiments, microfabrication platforms are provided to support the transmission of dynamic electromechanical forces, such that the cardiac microtissue constructs may be formed mimicking the basic microenvironment found in the heart. The microfabrication platform may include retaining features for stabilizing the position of the microtissue construct during its formation, and the microfabrication platform may include a ramped support configured to produce tissue constructs having a ring geometry. In some embodiments, the microfabrication platform may be configured to for the application of point electrical stimulation, and/or to amplify the transduction of force into a visible displacement.
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116.发明申请公开(公告)号：US20180112205A1
公开(公告)日：2018-04-26
申请号：US15572424
申请日：2015-05-08
申请人： INDIBA, S.A.
IPC分类号： C12N13/00 , C12N5/0775
CPC分类号： C12N13/00 , C12N5/0667 , C12N2529/00
摘要： In vitro method for stem cell proliferation and use of a device for increasing the proliferation of stem cells in vitroThe invention relates to an in vitro method for the proliferation of stem cells, comprising a step in which the stem cell culture is treated with an alternating current with a frequency in the 0.4 MHz to 0.6 MHz range, and to the use of a device for generating alternating current with a frequency in the 0.4 MHz to 0.6 MHz range in order to increase the proliferation of stem cells in vitro.
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117.发明授权公开(公告)号：US09868938B2
公开(公告)日：2018-01-16
申请号：US14995997
申请日：2016-01-14
发明人： Yi-Gang Wang , Ha Won Kim , Ruilian Ma
CPC分类号： C12N5/0657 , A61K35/34 , C12N5/0696 , C12N2506/45 , C12N2529/00
摘要： Methods of generating cardiomyocytes from induced pluripotent stem cells (IPSCs) are provided. More specifically, the present disclosure relates to methods of generating cardiomyocytes from iPSCs using electrical stimulation. In some aspects, uses of such cells for therapeutics and in methods of treatment are provided.
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公开(公告)号：US20170362571A1
公开(公告)日：2017-12-21
申请号：US15694635
申请日：2017-09-01
发明人： Vasile Bogdan Neculaes , Juan Jose Becerra , Christine Angela Morton , Andrew Soliz Torres , Antonio Caiafa
CPC分类号： C12N5/0644 , C12M35/02 , C12N2529/00
摘要： Methods and systems for generating a tunable or customizable activated product composition are related. In certain embodiments, one or more of electric pulse parameters, flow rate, or sample container size are varied so as to generate the activated product composition. The activated product composition may be customized or optimized based for a particular patient or procedure.
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公开(公告)号：US20170360836A1
公开(公告)日：2017-12-21
申请号：US15685086
申请日：2017-08-24
发明人： Shai NOVIK , Dror MEVORACH
IPC分类号： A61K35/17 , C12N5/0783 , A61K45/06 , A61K39/00 , C07K16/28 , A61K39/395
CPC分类号： A61K35/17 , A61K39/0011 , A61K39/39541 , A61K39/39558 , A61K45/06 , A61K2039/505 , A61K2039/5158 , C07K16/2887 , C12N5/0636 , C12N2501/04 , C12N2501/91 , C12N2523/00 , C12N2529/00 , A61K2300/00
摘要： Compositions disclosed herein, and methods of use thereof included those for inhibiting or reducing the incidence of cytokine release syndrome or cytokine storm in a subject undergoing CAR T-cell therapy, methods of treating a cancer or tumor, methods of reducing tumor load, methods of reducing the size or growth rate of a cancer or a tumor, and methods of extending of the survival of a subject suffering from a cancer or tumor, wherein the subjects are administered compositions comprising apoptotic cells or apoptotic cell supernatants. Compositions and methods of use thereof may increase the efficacy of a CAR T-cell cancer therapy. Disclosed herein are also compositions and methods of use thereof for decreasing or inhibiting cytokine production in a subject experiencing cytokine release syndrome or cytokine storm. In certain instances compositions may include additional chemotherapeutic or immunomodulatory agents.
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公开(公告)号：US20170355954A1
公开(公告)日：2017-12-14
申请号：US15668256
申请日：2017-08-03
CPC分类号： C12N5/0068 , A61K35/12 , A61K35/28 , A61L27/16 , A61L27/3834 , C12N11/08 , C12N13/00 , C12N2529/00 , C12N2533/30 , C08L27/16
摘要： A method of manufacturing and/or using a scaffold assembly for stem cell culture and tissue engineering applications is disclosed. The scaffold at least partially mimics a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation that uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface; electrospinning a first substantially aligned polymer fiber layer onto the first surface; providing a second pre-electroded substrate surface; electrospinning a second substantially aligned polymer fiber layer onto the second surface; and, retaining together the layered surfaces with a clamp and/or an adhesive compound.
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